Mid1 (NM_022927) Rat Untagged Clone

CAT#: RN215383

Mid1 (untagged ORF) - Rat midline 1 (Mid1), (10 ug)



  "NM_022927" in other vectors (3)

CNY 8,650.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Fxy; Midline1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN215383 representing NM_022927
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC
CCTGTGCACATAGCCTCTGTTTCAACTGTGCCCACCGCATCCTGGTCTCTCACTGTGCCACCAACGAGCC
TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA
GACGGGCTCAAGCGCAACGTCACCTTACAAAACATCATTGACAGGTTTCAGAAAGCATCCGTGAGTGGGC
CCAATTCTCCCAGTGAGACCCGCAGGGAGCGGGCCTTTGACGCCAACACCATGTCTTCTGCCGAGAAAGT
TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACCTGTGTTACTTGCGAAGTGTCC
TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
CAATCCCGGACTCGCACATCCGGGGGCTAATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG
TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA
GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAATTTGGAGAGTAATCTCACCAATCTTATTAAGAGAA
ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAGGTCAATGCATCCCG
TCAAGAAGCCAAGTTGACAGAGGAATGTGATCTTCTCATTGAAATTATTCAGGAAAGAAGACAAATTATT
GGAACAAAGATTAAAGAAGGCAAGGTGATGAGGCTCCGCAAATTAGCTCAGCAGATTGCAAACTGTAAAC
AATGCATTGAAAGGTCTGCATCACTCATTTCCCAAGCAGAACACTCACTGAAGGAGAATGACCATGCCCG
TTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTCCTAATT
CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGGGAGAAGAAACTGCTAG
AATGTTTGGATTACCTAACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACGGCTTCCTATGA
CACCATCACTGTCCACTGGACTTCGGATGATGAGTTCAGTGTGGTCTCCTATGAACTCCAGTACACCATA
TTCACTGGACAAGCCAATGTCGTTAGTCTTTGTAACTCGGCAGATAGCTGGATGATTGTGCCCAACATCA
AGCAGAACCACTATACTGTGCATGGCCTACAAAGTGGCACCAAGTATATCTTCATGGTCAAGGCCATCAA
CCAGGCAGGCAGCCGCAGCAGTGAGCCTGGGAAGCTGAAGACAAACAGTCAGCCATTTAAACTGGATCCC
AAATCTGCTCATCGAAAACTCAAGGTGTCCCATGACAACCTGACTGTTGAACGTGATGAGTCATCATCCA
AAAAGAGTCACACACCTGAACGCTTCACTAGCCAAGGGAGCTATGGCGTGGCTGGAAATGTGTTTATTGA
CAGTGGCCGTCATTACTGGGAAGTGGTCATAAGCGGAAGCACATGGTATGCCATTGGCCTTGCGTACAAA
TCAGCTCCAAAACATGAATGGATTGGGAAGAATTCTGCTTCCTGGGCCCTTTGCCGCTGCAACAATAACT
GGGTGGTGAGACATAACAGCAAGGAAATACCCATTGAACCAGCCCCCCACCCCCGGCGTGTAGGCATCCT
GCTGGACTATGATAATGGCTCAATCGCTTTTTATGATGCTTTGAACTCCATCCACCTCTATACCTTCGAT
GTGGCGCTTGCGCAGCCTGTGTGCCCCACCTTTACTGTGTGGAACAAGTGTTTGACGATTATAACTGGTC
TTCCCATCCCAGACCATTTGGACTGTACAGAACAGCTACCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_022927
Insert Size 2004 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022927.1, NP_075216.1
RefSeq Size 2262 bp
RefSeq ORF 2004 bp
Locus ID 54252
UniProt ID P82458
Gene Summary human homolog forms homodimers which associate with microtubules in the cytoplasm and mutations in gene are associated with Opitz syndrome [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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