Dapk1 (NM_001107335) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215317 representing NM_001107335 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGTGTTCAGGCAGGAAAACGTGGACGACTACTACGACACCGGCGAGGAACTGGGCAGCGGACAGT TCGCCGTTGTGAAGAAATGTCGTGAGAAAAGTACCGGTCTTCAGTATGCGGCCAAGTTCATCAAGAAAAG GAGGACCAAGTCCAGCCGGCGGGGCGTGAGCCGAGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG ATCCGGCACCCAAACGTCATCACCCTGCATGAGGTCTACGAGAACAAGACAGACGTCATTCTGATCCTGG AGCTTGTTGCAGGAGGCGAGCTGTTTGACTTCCTGGCTGAGAAGGAATCTCTCACTGAAGAAGAAGCAAC CGAGTTCCTCAAGCAGATCCTCAGCGGCGTTTACTACCTGCACTCGCTTCAAATCGCCCACTTTGACCTG AAGCCGGAAAACATAATGCTTCTGGATAGAAATGTGCCCAAACCTCGGATCAAGATCATCGACTTCGGCC TGGCCCATAAAATTGACTTTGGAAATGAATTCAAAAACATATTTGGGACACCAGAGTTTGTGGCTCCGGA GATCGTCAACTATGAGCCCCTTGGTCTTGAGGCAGACATGTGGAGCATTGGGGTAATAACCTATATCCTC CTAAGCGGGGCCTCCCCTTTTCTTGGAGACACCAAGCAAGAAACATTAGCGAATGTATCTGCTGTCAACT ATGACTTTGAGGAGGAATTCTTCCGCAACACCAGTACCCTTGCCAAAGATTTCATCAGAAGGCTGCTGGT CAAGGATCCAAAGAAGAGGATGACAATCCAGGACAGTTTGCAGCACCCCTGGATCAAGCCTAAAGACACC CAACAAGCACTTAGTCGAAAAGCCTCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCTCGGAAAA AATGGAAACAATCTGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTTTTGTCCAGAAGTAA CATGAGCGTTGCCAGGAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATTCAT GCCATCAATGATGACAACGTCCCCGGCCTGCAGCACCTCCTGGGCTCATTGTCCAGCTATGATGTCAACC AGCCCAACAAGCACGGGACACCTCCATTACTGATTGCTGCAGGCTGTGGGAATATTCAGATGTTACAGTT ACTCATAAAACGGGGCTCAAGGATCGACGTCCAGGATAAGGGAGGATCCAATGCTATCTACTGGGCCTCT CGGCATGGCCATGTGGATACTTTGAAGTTTCTCAATGAGAACAAATGCCCTTTGGATGTTAAAGACAAGT CTGGAGAGACAGCTCTTCACGTGGCAGCCCGCTATGGCCATGCAGATGTGGTTCAACTCTTGTGCAGTTT TGGCTCTAATCCTGATTTCCAGGACAAGGAAGAGGAAACCCCCCTGCACTGTGCTGCCTGGCACGGCTAT TACTCCGTGGCTAGAGCTCTTTGTGAAGTTGGCTGTAACGTGAATATCAAGAACCGGGAGGGAGAGACCC CACTGCTGACAGCTTCTGCCAGGGGCTACCATGACATTGTGGAGTGTCTGGCTGAACATGGAGCTGACCT GAATGCTTCTGACAAGGATGGGCACATCGCCCTTCATCTTGCTGTGAGGCGCTGTCAGATGGAGGTCATC AAGACCCTCCTTGGCCATGGGTGCCTTGTGGATTTCCAGGATCGGCATGGCAACACCCCCCTGCATGTGG CCTGCAAAGATGGGAGCGCGCCCATCGTGGTGGCCCTCTGTGAAGCCAGCTGCAACCTGGACATCTCAAA CAAGTATGGTCGGACTCCTCTCCACCTTGCAGCCAACAACGGGATCCTAGATGTGGTCCGCTACCTCTGC CTGATGGGCGCCAACGTGGAGGCTCTGACCTTGGATGGCAAGACGGCCGAGGACCTCGCCAAGGCAGAGC AGCACGAGCACGTGGCAGGGCTCCTGGCAAGACTGCGGAAGGACACACACCGAGGGCTCTTCATCCAGCA GCTCCGACCCACGCAGAATCTCCAGCCCAGAATCAAGCTCAAACTGTTTGGCCACTCGGGATCGGGGAAA TCCACACTGGTGGAATCTCTCAAGTGTGGGCTGTTAAGGAGTTTCTTCAGAAGGCGCCGGCCCAGACTGT CCTCTACCAACTCCACCCGCTTCCCACCGTCGCCCCTGGCTACTAAGCCAACAGTCTCAGTGAGCATTAA CAACCTGTACCCCGGCTGTGAGAATGTGAGCGTGAGGAGCCGAAGCATGATGTTTGAGCCAGGCCTCACC AAAGGGATGCTGGAAGTGTTCGTGGCTCCGTCTCACCACCTACACTGCTCGACCGATGACCAGTCCACCA AAGCCATCGACATCCAGAATGCTTACTTGAACGGAGTTGGTGATTTCAGCGTATGGGAGTTCTCTGGAAA CCCTGTGTACTTCTGTTGCTATGACTACTTCGCCGCCAACGATCCCACATCCATCCACATCATCGTTTTC AGTCTCGAAGAACCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTTAGTTTCTTGAAGTCTCTGG TCTCAGTTGAAGAACCCATAGCATTTGGAGGCAAGCTGAAGAACCCTCTCCGAGTTGTCCTGGTGGCCAC ACATGCTGACATCATGAACATCCCTCGGCCTGCTGGGGGCGAGTTTGGATATGATAAAGATACATCCTTG CTGAAAGAGATCAGGAACAGGTTTGGGAATGACCTTCATGTCTCAAATAAGCTGTTTGTGCTGGATGCAG GAGCATCTGGGTCTAAGGACATCAAGGTTCTCCGGAATCACCTGCAAGAAATACGGAGTCAGATTGTCTC GGGGTGCCCTCCCATGACTCATCTGTGTGAGAAGATCATCTCCACTCTGCCCTCCTGGCGGAAGCTCAAC GGGCCAAACCAGCTGATGTCGCTCCAGCAGTTTGTGTATGACGTGCAGGACCAGCTGAACCCCCTGGCCT CCGAGGATGACCTCAGGCGCATTGCACAGCAGCTGCACAGCACGGGAGAGATCAACATCATGCAGAGTGA GACAGTGCAGGATGTGTTGCTGCTGGATCCTCGATGGCTCTGCACCAACGTCCTGGGGAAGCTGCTGTCT GTGGAGACTCCCCGAGCCCTGCACCATTATCGGGGCCGCTACACCATGGAAGATATCCAGCGTCTGGTCC CCGACAGTGACGTGGAGGAGCTGCTGCAGATCCTGGATGCCATGGATATCTGCGCCCGAGACCTGAGTAG CGGGACTATGGTGGATATCCCTGCTCTAATCAAAACAGACAGCCTGCAGCGCTCCTGGGCCGATGAGGAA GACGAGGTGATGGTGTACGGAGGTGTGCGCATCGTCCCTGTAGAGCACCTCACTCCCTTCCCCTGTGGCA TCTTTCACAAAGTTCAGGTCAACCTGTGCCGGTGGATCCACCAGCAGAGCACTGAGGGGGACGCGGACAT CCGTCTGTGGGTGAGCGGCTGCAGGATCGCCAACCGTGGGGCTGAGTTGCTGGTGCTGCTGGTCAATCAC GGTCAGGGCATTGAGGTACAGGTGCGTGGACTGGAGACCGAGAAGATTAAGTGCTGCCTGCTGCTGGACT CGGTGTGCAGCACCATCGAGACTGTCATGGCCACCACCTTGCCAGGGCTGTTGACGGTGAAGCACTACCT GAGCCCCCAGCAGCTAAGGGAGCATCACGAACCAGTCATGGTCTACCAGCCCCGGGACTTCTTCCGTGCG CAGACCCTGAAAGAGAGCTCCCTCACCAACACCATGGGAGGGTACAAGGAGAGCTTCAGCAGCATCACGT GCTTTGGGTGTCACGATGTCTACTCACAGGCCAGTCTTGGCATGGACATCCATGCATCAGACCTGAGTCT CCTGACCCGGAGGAAACTGAGTCGTCTTCTTGACCCACCGGACCCCATGGGGAAGGACTGGTGCCTTCTG GCCATGAACTTGGGCCTCCCGGACATGGTGGCCAAACACAACGTCAATACCAGGGCTTCTAGGGATTTCC TCCCCAGCCCAGTGCATGCCTTGCTACAGGAATGGACCTCCTACCCTGAGAGCACGGTGGGCATCCTTAT ATCCAAACTTCGGGAGCTGGGGCGCCGGGATGCTGCGGACTTTTTACTGAAGGCCTCCTCTGTGTTCAAG ATCAACCTTGACGGCAATGGCCAGGAGGCCTATGCCTCAAGCTGTAACAGTGGCACATCCTACAATTCCA TAAGCTCAGTGGTGTCCCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107335
Insert Size	4293 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107335.2, NP_001100805.2
RefSeq Size	5878 bp
RefSeq ORF	4293 bp
Locus ID	306722

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