Edf1 (NM_001106557) Rat Untagged Clone

CAT#: RN215309

Edf1 (untagged ORF) - Rat endothelial differentiation-related factor 1 (Edf1), (10 ug)



  "NM_001106557" in other vectors (3)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms CAP-19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN215309 representing NM_001106557
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAGTGACTGGGACACCGTGACGGTGCTGCGCAAGAAGGGCCCCACGGCCGCACAGGCCAAGT
CTAAGCAGGCCATCTTAGCAGCTCAGCGACGAGGAGAAGATGTGGAGACATCCAAAAAATGGGCTGCTGG
CCAGAACAAGCAGCATTCCATCACTAAAAACACAGCCAAGCTAGACCGGGAGACAGAGGAGCTGCACCAC
GACAGGGTGACCCTGGAGGTGGGCAAAGTGATCCAGCGGGGCCGGCAGAGCAAAGGCCTGACGCAGAAGG
ACCTGGCCACGAAAATCAATGAAAAGCCGCAAGTCATCGCAGACTATGAAAGTGGACGGGCCATTCCGAA
TAACCAGGTTTTGGGCAAAATTGAGAGAGCCATCGGCCTCAAGCTCCGGGGGAAGGACATCGGGAAGCCT
ATCGAGAAGGGGCCGAAGGCGAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001106557
Insert Size 447 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001106557.1, NP_001100027.1
RefSeq Size 685 bp
RefSeq ORF 447 bp
Locus ID 296570
UniProt ID P69736
Gene Summary Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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