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Fto (NM_001039713) Rat Untagged Clone
CAT＃: RN215259
Fto (untagged ORF) - Rat fat mass and obesity associated (Fto), (10 ug)
CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1305121 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN215259 representing NM_001039713
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGCGTCCAGACCGCGGAGGAACGGGAGCGGGAAGCTAAGAAACTGAGGCTCCTCGAGGAGCTTG AAGACACTTGGCTTCCTTACCTGACCCCCAAAGATGACGAGTTCTATCAGCAGTGGCAGCTGAAATATCC TAAACTGGTTTTCCGAGAGGCTGGCAGCATACCCGAGGAGCTGCACAAAGAGGTCCCCGAGGCCTTTCTC ACACTGCACAAGCATGGCTGCTTGTTTCGGGACCTGGTGAGGATCCAAGGCAAAGACGTGCTCACCCCGG TGTCTCGCATCCTCATTGGGGACCCCGGCTGCACCTACAAGTACTTGAACACCAGGCTCTTCACCGTGCC CTGGCCAGTGAAGGGCTGCACCATCAATTACACAGAGGCCGAGATTGCCGCCGCATGTCAGACCTTCCTC AAGCTCAATGACTACCTACAGGTCGAGACCATCCAGGCCTTGGAAGAACTGGCTATCAAAGAGAAGGCCA ATGAAGACGCTGTGCCGTTGTGCATGGCAGAGTTCCCCAGGGCTGGCGTGGGACCGTCCTGCGATGATGA AGTGGACCTTAAGAGCAGAGCAGCCTACAACGTGACTTTGCTAAACTTCATGGATCCTCAGAAAATGCCG TACTTGAAAGAGGAGCCCTATTTCGGCATGGGGAAGATGGCGGTGAGCTGGCACCATGACGAGAACTTGG TGGACAGGTCAGCCGTGGCGGTGTACAGCTATAGCTGTGAAGGCTCCGAGGATGAAAGCGATGACGAGTC CAGCTTCGAAGGCAGAGATCCCGATACGTGGCATGTTGGTTTTAAGATCTCATGGGACATCGAGACGCCA GGCTTGACAATTCCTCTTCACCAGGGAGACTGCTATTTCATGCTGGATGACCTCAATGCCACCCACCAGC ACTGTGTTTTGGCTGGCTCACAGCCTCGGTTTAGCTCCACCCACCGCGTGGCAGAGTGCTCAACAGGCAC CTTGGATTATATCTTACAACGCTGCCAGTTGGCACTGCAGAATGTTCTCAATGACTCGGACAATGGCGAC GTCTCGCTGAAGTCCTTCGAGCCTGCAGTTCTGAAACAAGGAGAAGAGATCCACAACGAGGTCGAGTTTG AGTGGCTGAGGCAGTTCTGGTTTCAAGGAAATCGATACAAAATTTGCACTGATTGGTGGTGTGAGCCCAT GACTCAGCTGGAGGGGCTGTGGAAGAAGATGGAGAGTGTGACAAATGCCGTGCTTCGTGAAGTTAAGAGA GAGGGGCTCTCCGTGGAACAAAGGAGTGAAATTCTGTCTGCCGTCCTGATCCCACTCACCATGCGCCAGA ATCTGAGGAAAGAATGGCACGCCAGGTGCCAGGCCCGAGTTGTCCGAACTCTACCAGCACAGCAGAAACC AGACTGCCGGCCATATTGGGAGAAGGATGACCCTTCTATGCCTCTGCCCTTTGACCTCACAGATGTGGTC TCTGAGATCAGAAGCCAGCTTCTGGAAGCAAGATCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001039713 |
| Insert Size | 1509 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039713.1, NP_001034802.1 |
| RefSeq Size | 1571 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 291905 |
| UniProt ID | Q2A121 |
| Gene Summary | RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (By similarity). Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR215259 | Fto (Myc-DDK-tagged ORF) - Rat fat mass and obesity associated (Fto), (10 ug) |
CNY 3,736.00 |
|
| RR215259L3 | Lenti ORF clone of Fto (Myc-DDK-tagged ORF) - Rat fat mass and obesity associated (Fto), (10 ug) |
CNY 8,360.00 |
|
| RR215259L4 | Lenti ORF clone of Fto (mGFP-tagged ORF) - Rat fat mass and obesity associated (Fto), (10 ug) |
CNY 9,120.00 |
