Notch4 (NM_001002827) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215069 representing NM_001002827 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCGCCAGTGGCTGCTGCTTCTGCTCTTGCCGCTCAGCTTCCCAGTCATCCTGACCAGAGAGCTTC TGTGTGGAGGATCCCCAGAGCCCTGTGCCAACGGAGGCACCTGCCTGAGGCTATCTCAGGGACAAGGGAC CTGCCAGTGTGCCCCTGGATTTCTGGGTGAGACTTGCCAGTTCCCTGACCCCTGCTGGGACACCCAACTC TGCGAGAACGGTGGCAGCTGTCAAGCCTTGCTCCCCACAGCCCCAAGCTCCCACAGCCCCACTTCTCCAC TGACCCCCCACTTCTCCTGCACCTGCCCCTCTGGCTTCACTGGTGATCGATGCCAAAGCCCTCTGGAAGA GCTCTGTCCGCCTTCTTTCTGTTCCAACGGGGGTCACTGCTCTGTCCAGGTCTCGGGCCGCCCACAGTGT TCCTGCGAGCCCGGGTGGACAGGTGAGCAGTGCCAGCTCCGAGACTTCTGCTCAGCCAACCCCTGTGCCA ACGGAGGTGTGTGCTTGGCCACATACCCCCAGATCCAGTGTCGTTGTCCAACCGGCTTCGAGGGTCACAT CTGTGAACGCGACGTCAATGAATGCTTCCTGGAGCCCGGACCCTGCCCTCGGGGCACCTCCTGTCACAAC ACCTTGGGCTCCTTCCAGTGCCTCTGCCCTGTGGGGCAGGAAGGTCCCCAGTGCAAGCTCAGGAAGGGAG CCTGCCTTCCTGGAACCTGTCTCAATGGAGGCACCTGCCAGCTGGTGCCAGAGGGAGACACTACCTTTCA TCTCTGCCTCTGTCCCCCAGGTTTCACAGGGCTGAACTGTGAGATGAATCCAGATGACTGTGTCAGGAAC CAGTGTCAGAACGGGGCCACCTGCCAGGATGGGCTGGGCACCTACACCTGCCTCTGCCCCAAGACGTGGA AGGGCTGGGACTGCTCCGAAGACATAGATGAATGTGAAGCCCAGGGTCCGCCTCGATGCAGAAACGGTGG CACCTGCCAGAACTCGGCTGGTGGCTTTCACTGTGTGTGTGTGAGTGGCTGGGGAGGCGAGGGCTGTGAC GAGAACCTGGATGACTGTGCGGCCGCCACCTGTGCCCTGGGATCCACCTGCATCGACCGTGTGGGCTCCT TCTCCTGCCTCTGCCCACCTGGACGCACAGGCCTCCTGTGCCACCTGGAAGACATGTGTTTGAGGCAGCC GTGCCATGTGAATGCCCAGTGCAGCACCAACCCCCTGACGGGGTCTACCCTCTGCATATGCCAGCCGGGC TACTCGGGACCCACCTGCCACCAAGATCTGGATGAGTGTCAAATGGCCCAGCAAGGACCCAGTCCCTGTG AACACGGCGGCTCCTGCATCAACACCCCTGGCTCCTTCAACTGCCTCTGCCTGCCTGGCTACACGGGCTC CCGCTGCGAGGCTGACCACAATGAGTGCCTGTCGCAGCCCTGTCACCCGGGCAGCACCTGCCTGGACCTG CTTGCCACCTTCCAGTGCCTCTGCCCACCAGGCCTGGAAGGGAGGCTCTGTGAAGTGGAGATCAATGAGT GTGCCTCTAACCCCTGCCTCAACCAAGCTGCCTGTCACGACCAGCTCAACGGCTTCCTGTGCCTCTGCCT TCCTGGATTCACTGGTGCCAGATGCGAGAAAGACATGGACGAGTGCAGCAGCGCCCCCTGTGCCAATGGG GGGCACTGCCAAGACCAGCCTGGAGCCTTCCACTGCGAGTGTCTCCCAGGCTTTGAAGGGCCACGCTGTG AGACTGAAGCGGATGAATGTCGGAGTGACCCCTGCCCCGTGGGAGCCAGCTGCCTTGATCTTCCCGGGGC GTTCCTCTGCCTCTGCCGGCCTGGTTTCACAGGTCAACTTTGTGAGGTTCCCTTGTGTAGCCCCATCCTG TGCCAACCTGGACAGCAATGCCAAGACCAGGAACACAGAGCCCCCTGCCTCTGCCCTGACGGAAGTCCTG GCTGTGTTCCTGCTGAGGACGACTGCCCCTGCCACCATGGCCATTGCCAGAGATCCTTGTGTGTGTGTAA CGAGGGCTGGACTGGACCAGAGTGTGAGACAGAGCTGGGCGGCTGCCTCTCCACACCCTGTGCCCATGGG GGGACCTGCCACCCACAGCCCTCTGGCTACAACTGTAGCTGCCTTGCAGGCTACACGGGGCTGACCTGTA GTGAGGAGATAACAGCTTGTCACTCAGGGCCCTGTCTCAATGGTGGCTCCTGCAGCATCCATCCTGAGGG CTACTCTTGCACCTGCCCTCCAAGTCACACAGGTCCCCACTGCCAGACTGCCGTGGACCACTGTGCGTCT GCCTCGTGTCTCAACGGGGGTACCTGCATGAGCAAACCTGGCACTTTCTTCTGCCACTGTGCCACTGGCT TCCAGGGGCTGCACTGTGAGAAGAAGATTCACCCCAGCTGTGCAGACAACCCCTGCAGGAACAAGGCAAC CTGCCAAGACACACCTCGAGGGGCCCGCTGCCTCTGCAGCCCTGGCTACACAGGAAGCAGCTGCCAGACT CTGATAGACTTGTGTGCCCGGAAGCCCTGTCCACACACTGCCCGATGCCTCCAGAGTGGGCCCTCCTTCC ACTGCCTGTGCCACCAGGGATGGACAGGGTCTCTCTGTGACCTCCCACTGTCCTGCCAGGCGGCCGCGAT GAGCCAAGGTGTAGAGATCTCCAACCTGTGCCAGAATGGGGGCCTCTGTATTGACACGGGCTCCTCCTAT TTCTGTCGCTGCCCTCCTGGATTCGAGGGCAAGTTATGCCAGGATACTGTGAACCCCTGCACGTCCAAGC CTTGCCTTCACGGGGCTACCTGTGTGCCCCAGCCCAACGGCTATGTCTGCCAGTGTGCCCCAGGCTATGA AGGACAGAACTGCTCAAAAGTACACGACGCTTGCCAGTCCGGGCCGTGCCACAACCATGGAACCTGTACC CCCAGGCCTGGAGGCTTCCACTGTGCCTGCCCTCCAGGTTTTGTGGGGCTGCGCTGTGAGGGGGATGTGG ACGAGTGTCTCGACCGGCCCTGCCACCCCTCGGGCACTGCGTCCTGCCATTCTTTAGCCAACGCCTTCTA TTGCCAGTGTCTGCCTGGGCACACAGGCCAGCGGTGTGAAGTGGAGATGGACCTCTGCCAGAGCCAGCCC TGCTCCAATGGAGGGTCCTGTGAGGTCACAACAGGACCGCCCCCTGGCTTCACCTGCCGCTGCCCCGAGG GTTTTGAAGGCCCCACCTGCAGCCGCAAAGCCCCTGCCTGCGGCAACCATCATTGCCACAACGGAGGCCT ATGTCTGCCCTCCCCTAAGCCAGGCTCCCCACCCCTCTGTGCCTGCCTCAGTGGCTTCGGGGGCCCTGAC TGTCTGACACCTCCAGCTCCACCAGGGTGCGGTCCTCCCTCCCCCTGCATGCACAATGGTAGCTGCACTG AGACCCCCGGGTTGGGCAATCCTGGCTTTCAATGCACCTGCCCTCCTGACTCTCCGGGGCCCCGGTGTCA AAGGCCAGGGGCGAATGGATGTGAGGGCCGAGGTGGCGATGGAGCCTGTGATGCTGGCTGCAGTGGCCCA GGAGGGGACTGGGATGGAGGGGACTGTTCCCTGGGGGTCCCAGACCCCTGGAAGGGCTGTCCCCCGCATT CCCAGTGCTGGCTTCTCTTCCGGGATGGGCGGTGCCACCCACAGTGCGACTCTGAGGAGTGTCTGTTTGA TGGCTATGACTGTGAGATCCCTCCAACTTGCACCCCAGCCTACGACCAGTACTGCCGAGATCACTTCCAC AACGGGCACTGTGAGAAAGGCTGCAACAACGCTCAGTGTGGCTGGGATGGGGGTGACTGCAGGCCAGAAG GGGATGACTCAGAGGGGGGCCCCTCCCTGGCCCTGCTGGTGGTGCTGAGTCCCCCAGCCTTGGATCAGCA GCTGCTTGCCCTGGCTCGAGTACTGTCCCTGACTCTGAGGGTCGGTCTCTGGGTGAGGAAGGACAGTGAA GGCAGGAACATGGTGTTCCCCTATCCTGGGACCCGGGCCAAAGAGGAGCTGAGTGGAACTAGAGATTCCT CTTCGTGGGAAAGACAAGCCCCTCACACTCAGACCCCGGGCAAAGAGACAGAGTCCCTTGGTGCAGGGTT TGTGGTAGTGATGGGAGTGGATTTGTCCCGCTGTGGTCCAGAGCATCCTGCGTCCCGCTGTCCCCGGGAC TCTGGGCTCCTGCTGCGTTTCCTTGCAGCAATGGCCGCAGTGGGAGCTCTGGAGCCCCTGCTGCCTGGAC CCTTGCTGGCTGCTCACCCTCAAGCAGGGACCGGGTCCCCCGCCACCCGGCTTCCCTGGCCCATTCTGTG TTCGCCAGTGGTCGGGGTGCTCCTCCTGGCCCTTGGGGCTCTTCTCGTCCTCCAGCTCATCCGGCGCCGG CGGCGGGAACACGGGGCCCTGTGGCTGCCCCCTGGTTTCATTCGAAGGCCACAGACACAGCAGGCACCCC ACCGGCGGAGGCCCCCACTGGGCGAGGACAACATTGGTCTCAAGGCGCTGAAGCCAGAGGCAGAAGTGGA TGAGGACGGAGTGGTCATGTGCTCGGGCCCTGAAGAGGGCGAGGCAGAAAAAACAGCCTCAGCCTCCAGG TGCCAGCTCTGGCCACTGCGCAGCGGCTGTGAAGGGCTCCCACAGGCAGTCATGCTGACCCCTCCTCAGG AGTGTGAATCAGAACTTCCAGATTCGGACACCTGTGGACCTGATGGGGTGACACCCCTGATGTCTGCCGT CTTCTGTGGGGGAGTGCAGTCCACCACTGTACAGAGACTGGGACTAGGAAATCCCGAACCCTGGGAACCA CTGCTGGATAGAGGGGCCTGCCCCCAGGCTCACACTGTGGGCACTGGAGAGACGCCCCTGCACCTAGCTG CCCGATTCTCTCGGCCAACTGCTGCCCGACGCCTCCTGGAGGCTGGAGCCAACCCCAACCAGCCAGACCG GGCTGGGCGTACCCCACTTCACACTGCAGTAGCTGCTGATGCTCGGGAGGTTTGCCAGCTCCTATTGGCC AGCAGACAGACCGCTGTAGACGCGCGCACAGACGACGGGACTACACCTTTGATGCTGGCTGCCAGGTTGG CCGTGGAGGACCTGGTTGAAGAATTGATCGCAGCCCGAGCAGATGTAGGAGCCAGAGATAAAAGGGGAAA AACTGCACTGCACTGGGCCGCTGCAGTGAACAACGCCCGAGCTGCCCGCTGTCTCCTCCAGACTGGAGCG GATAAAGATGCCCAGGACAGTAGGGAGCAGACGCCGCTGTTCCTGGCTGCGCGCGAAGGCGCCGTGGAGG TGGCACAGCTGCTGCTGGAGATCGGGGCAGCGCGGGGGCTGCGGGACCAGGCCGGGCTGGCCCCAGCAGA TGTGGCCCGCCAGCGTAGTCATTGGGACCTGCTGACGCTGCTGGAGGGGGCAGGACCGATCACGCAGGAG GCCCGTTCGCACGCACGCAACACGCCGGGGGGCGGGGCCGCGCCGCGCTGCCGGACGCTGTCTGCCGGAG CGCGCCCGCGCGGGGGCGGAGCTTGTCTGCAAGCTCGCACTTGGTCGGTGGACTTGGGAGCGCGCCGAGG GGCGGTATACGCGCGCTGCCGGAGCCGATCAGGAGGCTCTGGAGGCCCCTCCATGCGCGGCCGCAGGCTT TCCGCGGACTCCCGTGGACGACGCGGGGCTAGAGTATCACAGGATGACTGGCCTCGGGACTGGGTGGCCC TGGAAGCCTGCGGCTCTGCCTGCAGTGCGCCGATCCCGCCTCCCAGCCTTACCCCGTCCCCAGAACGCGG ATCCCCTCAAGTTTCCTGGGGTCTTCCAGTCCACCAAGAGGTCCCCTTAAACTCGGGTGGAAGGAATCAG AACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001002827
Insert Size	5886 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001002827.1, NP_001002827.1
RefSeq Size	6483 bp
RefSeq ORF	5886 bp
Locus ID	406162

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