Scaf8 (NM_139094) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rbm16
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214813 representing NM_139094 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGTGAAGACCTTCAACAGCGAGTTGTATTCGCTGAATGACTATAAGCCACCCATTTCTAAAG CAAAAATGACCCAGATTACAAAGGCAGCCATCAAAGCTATTAAGTTCTATAAACATGTGGTACAGAGCGT TGAGAAATTCATTCAGAAATGTAAACCAGAATACAAGGTACCCGGACTGTATGTTATTGACTCCATTGTG CGACAATCCCGGCATCAGGTTGGTCAAGAAAAGGATGTGTGTGCACCCAGATTTAGTAATAACATCATTA GCACTTTCCAGAATTTATATCGGTGCCCGGGGGATGACAAGAGTAAAATAGTGAGAGTATTAAACTTATG GCAGAAGAATAATGTTTTTAAGAGCGAGATTATCCAGCCTCTCCTGGATATGGCGGCAGGGATTCCTCCC CCAGTTGTCACCCCGGTTTTGGCCAGCACCACTGTAGCCATGAGCAACACTCCAGGAACACCTGTGACAC CTGTTACTCCAGCCAATGTGGTTCAAGGCCTACCTGATCCATGGGTATCGCAGATAACAAACACAGACAC ACTGGCAGCTGTTGCCCAGATCCTACAAAGTCCTCAAGGTCAACAGCTTCAGCAACTAATACAGACCTTA CAGATACAACAGCAGAAGCCCCAGCCCTCTATCCTTCAGGCTTTGGATGCTGGCCTTGTGGTTCAGTTGC AGGCACTCACTGCACAGCTTACAGCTGCAGCTGCTGCAGCCAACACACTTACTCCCTTGGACCAGGGAGT CTCTTTCAACAAGAAGTTGATGGATAGATTTGATTTTGGGGAAGATTCTGAGCATAGTGAAGAATCCAAA AAGGAAATTCCTACTCCTCAACTCTCTCACGTTTCTGAGTCTGTGAACAACTCCATCTTCCATCAGATAG CAGAACAGCTGCAGCAGCAGAACCTGGAGCAGTTGCGGCAGCAGCTCCTGGAGCAGCAGCAGCCTCAGAA GGTAACTCCTCAGGACAGCCAGGAGGGAGCATTTGGCTCTGAGCACTCAGCTTCACCCTCACAAGGGAGT AGCCAACAGCATTTTCTGGAACCTGAAGCAAATTTAGATGATTCCATAGACATTCAGCAACAGGATATGG ATATTGATGAAGGGCAAGATGTTGTTGAAGAGGAGATCTTTGAACCAGAAGCTAAGAAAGTAGCTGTTCG CTCAAGATCAAGAACTCATTCACGATCTCGATCAAGGTCACCAAGAAAACGGAGGTCCAGGTCACGCTCT GGGTCCCGAAAGCGAAAGCATAGGAAGAGGTCTCGCTCCCGCTCCAGAGAGAGAAAGAGAAAATCCTCAC GTTCCTATTCAAGTGAACGCAGAGCCAGGGAAAGGGAGAAAGAACGCCAAAAAAAGGGCTTGCCTCCTGT CAGATCGAAAACGCTAAGTGTATGCAGCACTACTCTCTGGGTTGGCCAAGTGGACAAGAAGGCTACACAG CAAGATTTAACCAACCTTTTTGAGGAGTTTGGGCAAATCGAATCCATTAATATGATTCCACCCAGGGGCT GTGCATATGTCTGTATGGTTCATCGACAAGATTCATTTCGGGCTCTCCAGAAACTGAGTTCAGGATCCTA TAAAATTGGTTCCAAGGTCATTAAGATTGCCTGGGCTTTGAACAAAGGTGTAAAGACAGAATACAAACAG TTCTGGGACGTGGATCTTGGAGTCACATATATACCCTGGGAAAAAGTTAAAGTAGATGACTTGGATGGCT TTGCAGAGGGAGGCATGATTGATCAGGAAACTGTAAATGCTGAATGGGAAACTGTGAAAACCTCAGAACC CGTTAAAGAGACTGTCCAGACGACACAGAGTCCAGCTGCAGTTGAGAAGGAGACAGTGGTCACAACGCAG TCAGAGGTTTTCCCTCCTCCTGTCACCATGCTGCAGATTCCTGTAGCACCAACCGTTCCTGCAGTTAGCT TAGTTCCACCAGCGTTTCCTGTGTCGATGCCTGTCCCCCCTCCTGGATTCAGCCCCATCCCTCCACCTCC TTTTCTACGAGCAAGTTTTAACCCTTCACAACCACCTCCTGGTTTTATGCCACCTCCAGTTCCCCCACCT GTGGTACCTCCCCCTGCAATCCCACCCGTAGTACCAACATCTTTAGTGCAACCACCATTGTCTATGACCC CAGAAACTGTGAAAGATGTTGGATTTGGCAGCCTTGTTTTACCAGGTGGTTCTGTTGCTGGCAATCTTGC TCCTTCAACTCTACCAGCTGGAAATGTTTTTAATCCTCCCAGTAAAGCAGAGCCTGAAGAAAAAGTACCT CACCTTACAGAACACCAGATTCCTTCTGGTGAGAATACCAGACCAGTGATTCCAAGTGATATTCCAAGCA GTGCTCCAATGTTAGCACAGCCGCCTGGGGCCTCAAACACCTCTGGGATCCTGTGCGTGCAGAGACCAAA CGTATCAAGCAATTCTGAAATTCTGGGGGTTCGTCCAGCCAATGTTTCCAACAGTGCTGCGATTATGGGA GCCCAGCCACCAAATATGCTCAATAACTCTGGAATTTTGGGCATACAGCCACCAAATGTGTCCAGTGGCT CTGGACTTCTTGGGGTACTGCCTCCAAACTTGCCTAACAACTCTGGACTTGTAGGACTACAGCCACCAAA TGTTACAAATCCTGCTGGACTTTTGGGAACACAGCCACCAATTGGACCTCAAAACTTACCCCCTTTAACC ATCCCTGCTCAAAGGATGCCTGCGTTGCCAATGTTAGACATTCGTCCGGGACTGATAGCACAGGCTCCTG GGCCAAGATTCCCTTTACTACAGCCTGGAATTCCACCACAACGGGGTATCCCTCCCCCATCGGTACTTGA CGCAGCTCTTCATCCACCACCCCGTGGACCTTTTCCTCCAGGGGATCTTTTTAGTCAGCCAGAAAGACCT TTTCTGGCTCCTGGAAGACCAAATATAGACAGTGTTCCTAACCCAGATAAAAGAATACCACTTGGGAATG ACAATATCCAGCAGGAAGGGGATAGAGATTACCGCTTTCCTCCTATAGAAACCAGGGAGGGCATTAATAG ACCTCCTCCAGTGGATGTTAGGGATGTGGTTGGACGACCTATAGATCCCAGAGAAGGCCCTGGTAGGCCT CCGTTAGATGGTAGGGATCATTTTGGAAGACCTCCAGTGGACATGAGAGAGACTCTTGTGAGGCCAGGTC TAGACCACCTTGGCCGAAGAGACCACTTTGGCTTTCCCCCAGAAAAGCCTTGGGGGCCTAGAGATTTCGA TGAGAGAGAGCACCGAGTTCTGCCTGTCTTTGGTGGTCCAAAGGGCTTACATGAAGAAAGAGGCAGATTT CGGGCTGGAAATTATCGATTTGATCCTAGAAGTGGTCCTTGGAACAGAGGATTTGGACAAGAAGTTCACA GAGATTTTGATGACCGCAGAAGACCCTGGGAGAGGCAGAGGGATAGGGATGACAGAGATTTTGATTTCTG CAGAGAAATTAATGGGAATCGTCTTGGACGAGATAGAATTCAAAACACTTGGGTTCCCCCTCCTCATGCT CGGGTTTTTGATTATTTCGAAGGGGCCACTTCTCAACGGAAAGGTGAGAATGTGCCTCAAGTTAATGGTG GAAATACAGAGAGACATGCTCCGCCACCGCCTTTGCCAGTGCAGAAGGACCCTGAACTCTATGAGAAGCT GGCGTCTTCAGGAGACGCAGACAAGGAGGAGAGCGGCACAGCTGCTGGTGTAGAGAGTGAAGCGGTGGTA GAAAGCACAGAGACTGAGGGGACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_139094
Insert Size	3807 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_139094.1, NP_620794.1
RefSeq Size	4794 bp
RefSeq ORF	3807 bp
Locus ID	245926
UniProt ID	Q63623
Gene Summary	binds the repetitive C-terminal domain (CTD) of RNA polymerase II; may act with other CTD binding proteins to link transcription and pre-mRNA processing [RGD, Feb 2006]

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