Ttyh1 (NM_001106225) Rat Untagged Clone

CAT#: RN214787

Ttyh1 (untagged ORF) - Rat tweety homolog 1 (Drosophila) (Ttyh1), (10 ug)



  "NM_001106225" in other vectors (3)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214787 representing NM_001106225
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCACCCCCGGGCTACCGACCCTCTGCCTGGGTGCATCTCCTCCACCAGCTGCCCCGTGCCGACT
TCCAGCTCCGCCCGGTGCCCAGCGGCTTTGCTCCCCGCGACCAAGAGTACCAGCAGGCCCTGCTGCTGGT
GGCAGCCTTGGCTGGGCTGGGTTTGGGCCTGAGCCTCATCTTCATCGCTGTCTACCTCATCCGCTTCTGC
TGCTGCAGGCCTCCAGAACCCCCTGGGGCCAAGAGTCCCCCACCCGGAGGAGGCTGTGTCACCTGGAGCT
GCATTGCTGCCCTTCTCGTGGGCTGTGCAGGCATTGGCATTGGTTTCTATGGTAACAGCGAGACCAGTGA
TGGGGTGTCCCAGCTCAGCTCAGCACTGCAGCATGCCAACCACACGCTCAGCACCATTGATGACTTGGTG
CTGGAAACGGTAGAGAGGCTGGGTGAGGCTGTGAGGACAGAGCTGACCACCCTGGAGGAAGTGTTGTCAG
AACGCGTGGAGCTGGTGGCTGCCACCCGGGGAGCCAGAAGGCAGGCTGAGGCAGCAGCTCAGCACCTGCA
AGGCCTAGCCTTCTGGCAGGGAGTGTCCCTGAGCCCAGTACAGGTGGCTGAGGATGTGACTTTCGTGGAG
GAGTACAGGTGGCTGGCCTATGTCCTCCTGCTGCTCTTGGTGCTTCTGGTTTGTCTCTTCACCCTCCTGG
GCCTGGCCAAGCAGAGCAAGTGGCTGGTGGTTGTGATGACCGCCATGAGTCTCCTGGTGCTTGTCCTGAG
CTGGGGTTCTATGGGCCTAGAGGCTGCCACAGCTGTGGGTCTCAGTGACTTCTGCTCCAACCCAGACACC
TATGTGCTGAACTTGACCCAGGAGGAGACAGGAATCAGTTCAGACATCCTCAACTATTATTTCCTGTGCA
ACCAGGCGGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGTGCTCTAGCCAGCATCCACTC
TCAGCTGCAAGGCCTGGAGCGGGAAGCTAGCCCTCAATTTCCTGCTGCACAGAAGCCCTTGTTATCCTTG
GAGGAGACGCTGAATGTGACAGAAAGAAGCTTCCACCAGTTGGTGGCACTGCTGCACTGCCGCAGTCTGC
ACAAGGACTATGGCTCAGCACTCCGAGGCCTGTGTGAAGATGCCCTAGAGGGCCTGCTGTTCCTGATGCT
CTTTTCTCTCCTGTCTGCCGGGGCCCTAGCCACCACTCTTTGCAGCCTGCCCCGTGCCTGGGCTCTCTTC
CCACCCAGTGATGACTACGATGACACAGACGATGACGACCCTTTCAACCCTCAGGAATCCAAGCGCTTTG
TGCAGTGGCAGTCTTCCATCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII     
ACCN NM_001106225
Insert Size 1353 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001106225.1, NP_001099695.1
RefSeq Size 2957 bp
RefSeq ORF 1353 bp
Locus ID 292597
UniProt ID P0C5X8
Gene Summary Probable chloride channel. May be involved in cell adhesion.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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