Rnf146 (NM_001012060) Rat Untagged Clone

CAT#: RN214781

Rnf146 (untagged ORF) - Rat ring finger protein 146 (Rnf146), (10 ug)



  "NM_001012060" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214781 representing NM_001012060
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATGATGGCCGGCTGTGGTGAAATTGATCACTCACTAAATATGCTTCCTACCAATAAGAAGGCGA
GTGAGACCTGTTCTAACACTGCACCTTCTCTAACAGTTCCCGAGTGTGCCATTTGTCTACAAACATGTGT
TCATCCAGTCAGTCTGCCCTGTAAGCATGTTTTCTGTTATCTGTGTGTAAAGGGCGCTTCATGGCTCGGG
AAGCGATGTGCTCTTTGTCGGCAAGAGATTCCTGAGGATTTTCTTGACAAGCCAACCTTGTTGTCACCAG
AAGAACTTAAGGCTGCAAGCAGAGGAAATGGTGAATATGTGTGGTATTATGAAGGAAGAAATGGATGGTG
GCAGTATGATGAGCGCACCAGTCGGGAGCTAGAAGATGCTTTTTCCAAAGGTAAAAAGAACACGGAAATG
TTAATTGCTGGATTTCTGTACGTTGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGAC
GTCGCAGAAAGATTAAAAGAGATATAATAGATATACCAAAGAAGGGAGTGGCTGGACTTCGGCTGGACTG
TGACAGCAACACTGTAAATCTAGCCAGAGAGAGTTCTGCCGATGGTGCGGACAGTGGGTCAGCACACACT
GGAGCTTCTGTGCAGCTTCCAGTGCCATCTTCTACAAGGCCTCTAACATCAGTCGATGGTCAGTTAACCA
GCCCTGTAACACCGTCCCCTGATGCAGGCGCTTCTTTGGAAGACTCTTTTGCTCATTTACAACTCAGTGG
AGACAGCATAGCTGAAAGGAGTCATAGAGGTGAAGGAGAAGAAGATCACGAATCACCATCTTCTGGTAGA
GTACCAGATACGTCCACTGAAGAAACAGAGTCGGATGCCAGTAGTGATATTGAGGATGCCCCTGTGGTGG
TTGCACAGCACTCCTTGACCCAACAGAGACTTTTGGTTTCAAGTGCAAATCAGACAGTAGCTGAACGGTC
TGACCGACCAGTTGCAGGGGGTGGGACCATGAGTGTCAATGTCAGATCCAGAAGGCCTGACGGACAGTGC
ACAGTGACAGAGGTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001012060
Insert Size 1068 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001012060.2, NP_001012060.2
RefSeq Size 1889 bp
RefSeq ORF 1068 bp
Locus ID 308051
UniProt ID Q5XIK5
Gene Summary E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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