Trpm4 (NM_001136229) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	LTrpC-4; Mls2s
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214731 representing NM_001136229 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGCAGGAGAAAGAGCAGAGTTGGATCCCTAAGATCTTCAGGAAGAAGGTGTGCACGACGTTCA TAGTGGACCTCCACGATGATGCGGGAGGGACCTTGTGCCAGTGTGGGCAACCTCGGGATGCCCACCCTTC TGTGGCTGTGGAGGATGCCTTTGGGGCAGCCGTAGTTACCGAGTGGAACAGTGATGAGCACACCACGGAG AAGCCCACAGATGCCTACGGGGACCTGGACTTCACATACTCTGGCCGAAAGTCCAGCAACTTCCTCCGGC TGTCTGATCGCACGGATCCGGCCACTGTGTACAGTCTCGTCACCCGCTCTTGGGGCTTTCGTGCTCCAAA CTTGGTGGTGTCAGTGCTGGGGGGGTCGGAGGGCCCCGTGCTACAGACCTGGCTGCAGGACCTTCTGCGT CGTGGGCTGGTGCGAGCAGCCCAGAGCACAGGGGCCTGGATCGTCACTGGGGGACTGCACACAGGCATTG GTCGGCATGTGGGTGTGGCTGTGAGGGACCACCAGACGGCCAGCACCGGGGGCAGCAAGGTGGTGGCCAT GGGTGTGGCCCCCTGGGGAGTGGTCCGGAACAGAGACATGCTGATCAACCCCAAGGGCTCATTCCCTGCA AGATACCGGTGGCGTGGTGACCCTGAGGACGGAGTTGAGTTCCCCCTGGACTATAACTATTCTGCTTTCT TCTTGGTGGATGATGGTACCTATGGCCGCATGGGTGGTGAGAACCGCTTCCGCCTTCGGTTTGAGTCCTA TGTGGCTCAGCAGAAGACCGGAGTGGGAGGGACTGGAATTGACATCCCTGTACTCCTTCTTCTCATCGAA GGCGATGAGAAGATGTTAAAGCGGATAGAGGATGCCACCCAGGCTCAGCTCCCCTGCCTCTTGGTGGCTG GCTCTGGGGGTGCTGCAGACTGCCTGGTGGAGACCCTGGAAGATACTCTGGCCCCAGGGAGTGGGGGACT CAGGCGAGGTGAAGCCCGGGATCGAATTAGACGTTACTTCCCTAAAGGAGACCCCGAGGTCCTGCAGGCC CAGGTAGAGAGGATCATGACCCGAAAGGAGCTGCTGACAGTCTATTCATCAGAGGACGGCTCCGAGGAGT TTGAGACTATCGTTTTGAGGGCTCTTGTGAAAGCCTGTGGGAGCTCCGAGGCCTCGGCTTACCTGGATGA ACTGCGTTTGGCTGTGGCTTGGAACCGCGTGGACATCGCCCAAAGTGAACTTTTCCGTGGGGACATCCAG TGGCGGTCCTTCCACCTGGAGGCCTCTCTCATGGACGCTCTGCTGAATGACCGGCCTGAGTTTGTGCGCT TGCTCATCTCCCATGGCCTTAGTCTGGGACACTTCCTGACCCCCGTGCGCCTGGCCCAGCTGTACAGCGC AGTGTCCCCCAACTCGCTGATCCGCAATCTTCTGGACCAGGCGTCCCACGCTAGTAGCAGCAAATCTCCA CCTGCAAATGGAGCTGCAGAGCTCCGGCCTCCTAATGTGGGGCAAGTTCTGAGGACTCTGTTGGGAGAAA CGTGTGCACCGCGGTACCCCGCCAGGAACACCCGACACTCCCTGCTGGGCCAGGACCACAGAGAGAATGA CTCTCTGCTCATGGACTGGGCCAACATGCAACAGGATGCAAGCTTTGAACAAGCCCCCTGGAGTGACCTG CTTATCTGGGCTCTGTTGTTGAACCGAGCCCAGATGGCCATTTATTTCTGGGAGAAGGGCTCCAACTCAG TGGCCTCTGCTCTCGGGGCTTGTCTCTTACTCCGAGTGATGGCTCGCCTTGAGTGGGAGGCTGAGGAAGC TGCACGGCGGAAGGACCTTGCTGCCAAGTTTGAAAGCATGAGCGTGGACCTCTTTGGAGAGTGTTACCAC AACAGTGAATATCGAGCAGCCCGCCTTCTTCTGCGTCGCTGTCCCCTCTGGGGAGAGGCCACCTGTCTCC AGCTTGCCATGCAGGCTGACGCCCGTGCGTTCTTTGCCCAGGATGGAGTACAGTCTCTGCTGACACAGAA GTGGTGGGGGGAGATGGACAGCACGAACCCTATCTGGGCCCTGCTTCTCACCTTCTTCTGCCCGCCTCTC ATCTACACCAACCTCATCCTCTTCAGGAAGTCAGAGGAGGAGCCCACACAGAAGGATCTTGACTTCGATA TGGACAGCAGCATGAATGGAGCAGGTCCTCTTGGGCCTGCGGAGCCCTCAGCAAAGGTGGCCCTTGAGAG GCGGCGGCGGCGGCGGCCAGGACATACCCTCTGCTGTGGCGGGTGCTCCAAGCGCTGGTCGTACTTTTGG GGCGCCCCAGTGACCGCCTTCCTGGGTAACGTGGTCAGTTACCTGCTGTTCCTGCTGCTGTTTGCACACG TGTTGCTGGTGGATTTCCAGCCTACAAAGCCGGGCGTCTTCGAGCTGCTGCTGTACTTCTGGGCCTTCAC GCTGCTATGCGAGGAGCTGCGCCAGGGCCTGGGCGGTGGCTGGGGTACCCTGGCCAACGGGGGACCTGGT CCTGGCAAAGCTCCCCTGCGCCATCGTCTGCACCTCTACCTCTTGGATACTTGGAACCAGTGCGACTTGC TGGCACTCACCTGCTTCCTGCTGGGCGTTGGCTGCAGGCTGACTCCTGGCCTGTTTGACTTGGGACGCAC AGTCCTCTGTCTTGACTTCATGATCTTCACACTGCGCCTGCTGCACATCTTCACGGTGAACAAGCAGCTG GGGCCCAAGATTGTCATCGTGAGCAAGATGATGAAGGATGTATTCTTTTTCCTCTTCTTCCTCTGCGTGT GGCTTGTAGCTTATGGGGTGGCCACAGAGGGGATCCTGAGGCCCCAGGACCGCAGTCTACCGAGCATCCT GCGCAGGGTCTTCTACCGTCCGTATCTGCAGATCTTCGGACAAATTCCCCAGGAGGAAATGGATGTGGCC CTCATGAATCCCAGTAACTGCTCTGCCGAGCGGGGCTCCTGGGCTCACCCGGAGGGGCCGGTCGCAGGCT CCTGTGTGTCCCAGTATGCCAACTGGCTGGTGGTGTTGCTCCTCATCGTCTTCTTGCTGGTGGCCAATAT CCTGCTGCTCAATCTGCTCATCGCCATGTTCAGCTACACCTTCAACAAAGTGCATGGCAACAGCGACCTC TACTGGAAGGCGCAGCGCTACAGCCTCATCCGGGAATTCCATTCGCGGCCTGCCCTGGCCCCACCCCTCA TCATCATCTCTCACCTGCGTCTCCTCTTCAAGTGGCTGCGCAGGTGTCATAGGACTAACTTGCCCGCCTC CCCAGTCTTCGAGCACTTCCGTGTCTGTCTCTCTAAGGAAGCAGAGAGGACGCTGCTGACTTGGGAGTCT GTGCACAAAGAGAACTTCCTATTGGCACAAGCTCGTGACAAGCGAGACAGCGACTCGGAGCGTCTGAAAC GCACGTCCCAGAAGGTGGACACTGCACTGAAGCAGCTGGGACAGATCAGAGAGTATGACCGGCGTCTGAG GGGGCTGGAGAGAGAGGTCCAACACTGTTCTCGAGTCCTGACTTGGATGGCTGAGGCCCTTAGCCACTCT GCCTTACTGCCTCCAGGGGGGCCACCCCCTCCAAGCCCCACTGGGTCCAAAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001136229
Insert Size	3627 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001136229.1, NP_001129701.1
RefSeq Size	4203 bp
RefSeq ORF	3627 bp
Locus ID	171143
UniProt ID	Q9ESQ5
Gene Summary	member of a family of calcium permeable channels [RGD, Feb 2006]

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