Trpm4 (NM_001136229) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LTrpC-4; Mls2s
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214731 representing NM_001136229 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGCAGGAGAAAGAGCAGAGTTGGATCCCTAAGATCTTCAGGAAGAAGGTGTGCACGACGTTCA TAGTGGACCTCCACGATGATGCGGGAGGGACCTTGTGCCAGTGTGGGCAACCTCGGGATGCCCACCCTTC TGTGGCTGTGGAGGATGCCTTTGGGGCAGCCGTAGTTACCGAGTGGAACAGTGATGAGCACACCACGGAG AAGCCCACAGATGCCTACGGGGACCTGGACTTCACATACTCTGGCCGAAAGTCCAGCAACTTCCTCCGGC TGTCTGATCGCACGGATCCGGCCACTGTGTACAGTCTCGTCACCCGCTCTTGGGGCTTTCGTGCTCCAAA CTTGGTGGTGTCAGTGCTGGGGGGGTCGGAGGGCCCCGTGCTACAGACCTGGCTGCAGGACCTTCTGCGT CGTGGGCTGGTGCGAGCAGCCCAGAGCACAGGGGCCTGGATCGTCACTGGGGGACTGCACACAGGCATTG GTCGGCATGTGGGTGTGGCTGTGAGGGACCACCAGACGGCCAGCACCGGGGGCAGCAAGGTGGTGGCCAT GGGTGTGGCCCCCTGGGGAGTGGTCCGGAACAGAGACATGCTGATCAACCCCAAGGGCTCATTCCCTGCA AGATACCGGTGGCGTGGTGACCCTGAGGACGGAGTTGAGTTCCCCCTGGACTATAACTATTCTGCTTTCT TCTTGGTGGATGATGGTACCTATGGCCGCATGGGTGGTGAGAACCGCTTCCGCCTTCGGTTTGAGTCCTA TGTGGCTCAGCAGAAGACCGGAGTGGGAGGGACTGGAATTGACATCCCTGTACTCCTTCTTCTCATCGAA GGCGATGAGAAGATGTTAAAGCGGATAGAGGATGCCACCCAGGCTCAGCTCCCCTGCCTCTTGGTGGCTG GCTCTGGGGGTGCTGCAGACTGCCTGGTGGAGACCCTGGAAGATACTCTGGCCCCAGGGAGTGGGGGACT CAGGCGAGGTGAAGCCCGGGATCGAATTAGACGTTACTTCCCTAAAGGAGACCCCGAGGTCCTGCAGGCC CAGGTAGAGAGGATCATGACCCGAAAGGAGCTGCTGACAGTCTATTCATCAGAGGACGGCTCCGAGGAGT TTGAGACTATCGTTTTGAGGGCTCTTGTGAAAGCCTGTGGGAGCTCCGAGGCCTCGGCTTACCTGGATGA ACTGCGTTTGGCTGTGGCTTGGAACCGCGTGGACATCGCCCAAAGTGAACTTTTCCGTGGGGACATCCAG TGGCGGTCCTTCCACCTGGAGGCCTCTCTCATGGACGCTCTGCTGAATGACCGGCCTGAGTTTGTGCGCT TGCTCATCTCCCATGGCCTTAGTCTGGGACACTTCCTGACCCCCGTGCGCCTGGCCCAGCTGTACAGCGC AGTGTCCCCCAACTCGCTGATCCGCAATCTTCTGGACCAGGCGTCCCACGCTAGTAGCAGCAAATCTCCA CCTGCAAATGGAGCTGCAGAGCTCCGGCCTCCTAATGTGGGGCAAGTTCTGAGGACTCTGTTGGGAGAAA CGTGTGCACCGCGGTACCCCGCCAGGAACACCCGACACTCCCTGCTGGGCCAGGACCACAGAGAGAATGA CTCTCTGCTCATGGACTGGGCCAACATGCAACAGGATGCAAGCTTTGAACAAGCCCCCTGGAGTGACCTG CTTATCTGGGCTCTGTTGTTGAACCGAGCCCAGATGGCCATTTATTTCTGGGAGAAGGGCTCCAACTCAG TGGCCTCTGCTCTCGGGGCTTGTCTCTTACTCCGAGTGATGGCTCGCCTTGAGTGGGAGGCTGAGGAAGC TGCACGGCGGAAGGACCTTGCTGCCAAGTTTGAAAGCATGAGCGTGGACCTCTTTGGAGAGTGTTACCAC AACAGTGAATATCGAGCAGCCCGCCTTCTTCTGCGTCGCTGTCCCCTCTGGGGAGAGGCCACCTGTCTCC AGCTTGCCATGCAGGCTGACGCCCGTGCGTTCTTTGCCCAGGATGGAGTACAGTCTCTGCTGACACAGAA GTGGTGGGGGGAGATGGACAGCACGAACCCTATCTGGGCCCTGCTTCTCACCTTCTTCTGCCCGCCTCTC ATCTACACCAACCTCATCCTCTTCAGGAAGTCAGAGGAGGAGCCCACACAGAAGGATCTTGACTTCGATA TGGACAGCAGCATGAATGGAGCAGGTCCTCTTGGGCCTGCGGAGCCCTCAGCAAAGGTGGCCCTTGAGAG GCGGCGGCGGCGGCGGCCAGGACATACCCTCTGCTGTGGCGGGTGCTCCAAGCGCTGGTCGTACTTTTGG GGCGCCCCAGTGACCGCCTTCCTGGGTAACGTGGTCAGTTACCTGCTGTTCCTGCTGCTGTTTGCACACG TGTTGCTGGTGGATTTCCAGCCTACAAAGCCGGGCGTCTTCGAGCTGCTGCTGTACTTCTGGGCCTTCAC GCTGCTATGCGAGGAGCTGCGCCAGGGCCTGGGCGGTGGCTGGGGTACCCTGGCCAACGGGGGACCTGGT CCTGGCAAAGCTCCCCTGCGCCATCGTCTGCACCTCTACCTCTTGGATACTTGGAACCAGTGCGACTTGC TGGCACTCACCTGCTTCCTGCTGGGCGTTGGCTGCAGGCTGACTCCTGGCCTGTTTGACTTGGGACGCAC AGTCCTCTGTCTTGACTTCATGATCTTCACACTGCGCCTGCTGCACATCTTCACGGTGAACAAGCAGCTG GGGCCCAAGATTGTCATCGTGAGCAAGATGATGAAGGATGTATTCTTTTTCCTCTTCTTCCTCTGCGTGT GGCTTGTAGCTTATGGGGTGGCCACAGAGGGGATCCTGAGGCCCCAGGACCGCAGTCTACCGAGCATCCT GCGCAGGGTCTTCTACCGTCCGTATCTGCAGATCTTCGGACAAATTCCCCAGGAGGAAATGGATGTGGCC CTCATGAATCCCAGTAACTGCTCTGCCGAGCGGGGCTCCTGGGCTCACCCGGAGGGGCCGGTCGCAGGCT CCTGTGTGTCCCAGTATGCCAACTGGCTGGTGGTGTTGCTCCTCATCGTCTTCTTGCTGGTGGCCAATAT CCTGCTGCTCAATCTGCTCATCGCCATGTTCAGCTACACCTTCAACAAAGTGCATGGCAACAGCGACCTC TACTGGAAGGCGCAGCGCTACAGCCTCATCCGGGAATTCCATTCGCGGCCTGCCCTGGCCCCACCCCTCA TCATCATCTCTCACCTGCGTCTCCTCTTCAAGTGGCTGCGCAGGTGTCATAGGACTAACTTGCCCGCCTC CCCAGTCTTCGAGCACTTCCGTGTCTGTCTCTCTAAGGAAGCAGAGAGGACGCTGCTGACTTGGGAGTCT GTGCACAAAGAGAACTTCCTATTGGCACAAGCTCGTGACAAGCGAGACAGCGACTCGGAGCGTCTGAAAC GCACGTCCCAGAAGGTGGACACTGCACTGAAGCAGCTGGGACAGATCAGAGAGTATGACCGGCGTCTGAG GGGGCTGGAGAGAGAGGTCCAACACTGTTCTCGAGTCCTGACTTGGATGGCTGAGGCCCTTAGCCACTCT GCCTTACTGCCTCCAGGGGGGCCACCCCCTCCAAGCCCCACTGGGTCCAAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214731 representing NM_001136229 Red=Cloning site Green=Tags(s) MVGQEKEQSWIPKIFRKKVCTTFIVDLHDDAGGTLCQCGQPRDAHPSVAVEDAFGAAVVTEWNSDEHTTE KPTDAYGDLDFTYSGRKSSNFLRLSDRTDPATVYSLVTRSWGFRAPNLVVSVLGGSEGPVLQTWLQDLLR RGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQTASTGGSKVVAMGVAPWGVVRNRDMLINPKGSFPA RYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRMGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLIE GDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQA QVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQ WRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSP PANGAAELRPPNVGQVLRTLLGETCAPRYPARNTRHSLLGQDHRENDSLLMDWANMQQDASFEQAPWSDL LIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLEWEAEEAARRKDLAAKFESMSVDLFGECYH NSEYRAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTNPIWALLLTFFCPPL IYTNLILFRKSEEEPTQKDLDFDMDSSMNGAGPLGPAEPSAKVALERRRRRRPGHTLCCGGCSKRWSYFW GAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPGVFELLLYFWAFTLLCEELRQGLGGGWGTLANGGPG PGKAPLRHRLHLYLLDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNKQL GPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMDVA LMNPSNCSAERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFNKVHGNSDL YWKAQRYSLIREFHSRPALAPPLIIISHLRLLFKWLRRCHRTNLPASPVFEHFRVCLSKEAERTLLTWES VHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAEALSHS ALLPPGGPPPPSPTGSKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001136229
ORF Size	3624 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001136229.1, NP_001129701.1
RefSeq Size	4203 bp
RefSeq ORF	3627 bp
Locus ID	171143
UniProt ID	Q9ESQ5
MW	135.3 kDa
Gene Summary	member of a family of calcium permeable channels [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.