Abcc4 (NM_133411) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mrp4; RGD1565953
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214716 representing NM_133411 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCGGTGCACACCGAGGTGAAACCCAACCCGCTGCAGGACGCCAACCTCTGCTCGCGCTTGTTCT TCTGGTGGCTCAACCCGTTGTTTAAAGCTGGACATAAGCGGAGATTGGAAGAAGATGACATGTTTTCAGT GCTTCCAGAAGATCGCTCAAAGCACCTTGGAGAGGAGTTGCAAGGGTACTGGGATAAAGAAGTTCTGCGA GCCAAGAAGGACGCTCGGAAGCCTTCCTTAACGAAGGCAATCGTGAAGTGTTACTGGAAATCTTACCTGA TTTTGGGAATTTTTACGTTAATTGAGGAGACCACCCGAGTAGTTCAGCCCATATTTTTAGGGAAAATTAT TGATTATTTTGAGAAGTATGACTCTGACGACTCGGCCGCTTTGCACACAGCTTACGGCTACGCGGCGGTG CTGTCGCTGTGCACGCTCATCCTGGCCATACTACATCACTTGTATTTCTACCACGTGCAGTGCGCCGGGA TGAGGATACGGGTCGCCATGTGCCACATGATTTACCGGAAGGCTCTCCGGCTAAGTAACTCGGCCATGGG GAAGACAACCACGGGCCAGATAGTCAACCTGCTGTCCAACGACGTGAACAAGTTTGACCAGGTGACCATC TTCTTGCACTTCCTGTGGGCGGGGCCGCTGCAAGCCATAGGGGTAACCATCCTTCTCTGGGTGGAAATCG GAATCTCCTGTCTGGCGGGCCTGGCCATTCTGGTTATTCTGCTGCCTCTGCAAAGCTGCATCGGGAAACT GTTCTCGTCACTGCGGAGTAAAACAGCGGCTTTCACGGATGCCAGGTTCCGGACCATGAATGAAGTGATA ACGGGCATGAGGATAATAAAGATGTACGCGTGGGAGAAGTCATTCGCCGACCTCATAACCAATCTGAGAA AGAAGGAAATTTCTAAGATTCTGGGCAGCTCCTACCTCAGAGGGATGAACATGGCGTCGTTTTTCATTGC AAACAAAGTCATCCTGTTCGTGACCTTCACCACCTATGTGCTGCTTGGCAATAAGATTACATCTAGCCAC GTGTTCGTGGCCATGACTCTGTATGGTGCTGTCCGGTTGACAGTTACCCTCTTCTTCCCATCAGCCATTG AGAGAGTGTCAGAGGCCGTCGTCAGCGTTCGGAGGATCAAGAACTTTCTGTTACTCGATGAACTGCCAGA GCGCAAAGCCCAGGAACCATCTGATGGCAAAGCCATCGTTCACGTGCAAGATTTTACCGCTTTCTGGGAC AAGGCATTAGACACTCCTACCCTGCAAGGTCTTTCCTTCACTGCCAGACCTGGTGAGTTGTTAGCTGTGG TCGGCCCAGTCGGAGCAGGCAAGTCGTCCCTGTTGAGCGCGGTGCTCGGTGAGCTGCCTCCCACCAGTGG GCTGGTCAGCGTGCACGGGAGGATCGCCTACGTTTCCCAGCAGCCCTGGGTGTTCTCAGGAACCGTGAGG AGCAATATTTTATTTGGGAGGAAATACGAGAAAGAACGCTACGAGAAAGTCATCAAGGCCTGTGCTCTGA AGAAGGACTTGCAACTTCTGGAGGACGGGGATCTGACTGTCATAGGCGACCGGGGAGCCACGCTGAGCGG AGGCCAGAAAGCACGGGTGAACTTGGCACGGGCAGTCTACCAGGATGCCGACATCTACCTCCTCGATGAT CCGCTCAGTGCTGTTGATGCAGAAGTGGGCAAGCACCTGTTCCAGCTGTGTATCTGCCAGACTTTGCACG AGAAGATCACCATTTTAGTGACTCACCAGTTACAGTACCTCAAAGCTGCAAGCCACATCCTCATACTAAA AGATGGCGAAATGGTGCAGAAGGGGACTTACACCGAGTTTCTGAAGTCTGGTGTAGATTTCGGTTCCCTG CTAAAGAAGGAAAATGAGGAAGCAGAGCCCTCCCCTGTCCCAGGAACACCGACACTCAGGAACCGAACCT TCTCCGAGGCCTCGATTTGGTCTCAACAGTCATCCAGACCCTCGTTGAAAGACGGGGTCCCAGATGCCCA AGATGCAGAGAATACTCAGGCAGCTCAACCCGAGGAGAGCCGTTCAGAAGGGAGAATCGGCTTCAAGGCC TACAAGAACTACTTCTCTGCAGGCGCATCCTGGTTTTTCATTATTTTCCTCGTTCTGCTTAACCTGATGG GTCAGGTTTTCTACGTTCTTCAGGACTGGTGGCTTTCCCACTGGGCGAATAGGCAAGGTGCCCTGAATGA CACCAAAAACGCGAACGGGAATGTAACCGGGACCCTCGATCTCAGCTGGTACTTAGGGATTTACACAGGT TTAACTGCGGTCACAGTCCTCTTTGGCATAGCGAGATCCCTGCTGGTGTTCTATGTCCTTGTGAATGCTT CCCAGACTCTGCACAACAGGATGTTCGAGTCGATCCTGAAGGCACCGGTGTTGTTCTTCGATAGAAATCC AATAGGAAGGATTTTAAATCGTTTCTCCAAGGACATCGGACACATGGACGACTTGCTTCCCCTAACGTTT CTAGACTTCATCCAGACCTTGCTCCTTGTTGTAAGTGTGATCGCCGTGGCGGCGGCCGTGATCCCTTGGA TCCTCATACCCCTGGTTCCACTCTCCATCATCTTTGTGGTCCTTCGGAGATATTTCTTAGAAACGTCGCG GGACGTCAAGCGCCTGGAGTCCACAACACGGAGCCCGGTATTCTCCCATTTATCATCCTCCCTCCAGGGA CTCTGGACCATCCGTGCCTATAAGGCTGAAGAGAGGTGTCAGGAGCTGTTTGATGCACACCAGGACTTGC ATTCAGAGGCTTGGTTCTTGTTCCTGACGACATCAAGATGGTTCGCCGTGCGTCTGGACGCCATCTGCGC CGTCTTTGTCATCGTCGTTGCCTTCGGGTCCCTGGTTCTGGCAAAGACCTTGGATGCTGGGCAGGTTGGC TTGGCCTTGTCCTACTCCCTCACGCTCATGGGGATGTTCCAGTGGTCTGTGAGACAGAGCGCCGAAGTAG AGAATATGATGATTTCTGTGGAGAGGGTGATTGAATACACGGACCTAGAGAAGGAGGCGCCTTGGGAATG CAGGAAGCGCCCACCCCCAGGCTGGCCCCACGAAGGAGTAATTGTCTTCGACAATGTGAACTTCACCTAC AGCTTGGACGGGCCCCTTGTTCTGAAGCACCTGACCGCACTCATCAAGTCACGAGAAAAGGTTGGAATTG TGGGAAGAACTGGAGCTGGAAAAAGTTCCCTCATCTCAGCCCTCTTCAGGTTGTCAGAACCTGAGGGGAA AATCTGGATTGATAAGATCTTGACGACTGAAATTGGACTTCACGATTTAAGGAAGAAAATGTCAATCATA CCTCAGGAACCTGTCCTGTTCACTGGAACCATGAGGAAGAACCTGGACCCCTTTAATGAGCACTCGGACG AGGAGCTGTGGAAAGCCCTAGAGGAGGTACAACTTAAAGAGGCCATAGAAGATCTTCCTGGTAAAATGGA CACTGAACTAGCAGAATCTGGATCCAATTTCAGTGTTGGACAGAGACAGTTAGTGTGCCTTGCGAGGGCA ATTCTGAAGAAAAACCGGATACTGATCATTGATGAAGCAACTGCAAATGTGGATCCGAGAACTGACGAGT TAATACAACAGAAAATCCGGGAGAAGTTTGCCCAGTGCACTGTTCTCACCATTGCTCACAGACTGAACAC CATCATTGACAGCGACAAGATAATGGTTTTGGATTCGGGAAGACTGAGAGAATATGATGAGCCGTATGTT TTGCTGCAGAATCCAGAGAGCCTCTTTTACAAGATGGTTCAGCAGCTGGGTAAGGGCGAAGCCGCTGCCC TCACCGAAACAGCAAAACAGGTGTACTTCAGACGGAATTATCCGGATATTGCATTCAGCAGCCCTGCGGT TATGAGCACCTCCAACGGACAGCCCTCCGCCTTAACGATATTTGAAACAGCATTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_133411
Insert Size	3978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133411.1, NP_596902.1
RefSeq Size	4526 bp
RefSeq ORF	3978 bp
Locus ID	170924
UniProt ID	Q6QMG6
Gene Summary	ATP-binding cassette (ABC) multiple drug resistance protein (MRP) that mediates reduced glutatione release from hepatocytes into blood by cotransport with monoanionic bile salts [RGD, Feb 2006]

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