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Ptbp1 (NM_022516) Rat Untagged Clone
CAT＃: RN214589
Ptbp1 (untagged ORF) - Rat polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, (10 ug)
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ptb; PTB1; Pybp; Pybp1; Pybp2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN214589 representing NM_022516
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCATCGTCCCAGACATAGCAGTCGGTACAAAGCGGGGATCCGACGAGCTCTTCTCCACGTGTG TCAGCAACGGCCCCTTCATCATGAGCAGCTCGGCCTCAGCAGCCAACGGAAATGATAGCAAGAAGTTCAA AGGTGATAACAGGAGCACAGGAGTTCCTTCCAGAGTCATCCATGTCCGAAAGCTACCTAGTGATGTCACT GAGGGCGAGGTCATCTCCCTGGGGCTGCCCTTTGGGAAAGTTACCAACCTCCTTATGCTGAAGGGGAAGA ACCAGGCCTTCATTGAAATGAGCACAGAGGAGGCTGCCAACACTATGGTTAACTACTACACATCGGTGGC GCCAGTGCTTCGTGGACAGCCCATCTACATCCAGTTCTCCAACCACAAGGAGCTCAAAACCGACAGCTCG CCCAACCAGGCACGTGCCCAGGCAGCCCTGCAGGCTGTGAACTCCGTACAGTCAGGAAACCTGGCCTTGG CAGCCTCTGCTGCTGCCGTGGATGCAGGAATGGCAATGGCAGGCCAGAGCCCAGTGCTCAGGATCATTGT GGAGAACCTTTTCTACCCAGTGACACTGGACGTGCTGCACCAGATCTTCTCGAAGTTTGGCACAGTCCTG AAGATCATCACGTTCACCAAGAACAACCAGTTCCAGGCGCTGCTGCAGTATGCTGACCCTGTGAGCGCCC AGCATGCCAAGCTGTCCCTGGATGGCCAGAACATCTACAATGCCTGCTGCACGCTGCGCATCGACTTCTC CAAGCTCACCAGTCTCAACGTCAAATACAACAACGACAAGAGCCGAGACTACACACGCCCTGACCTGCCC TCTGGAGACAGCCAGCCATCACTGGATCAGACCATGGCAGCAGCCTTTGGCCTCTCTGTTCCTAATGTCC ATGGAGCCCTGGCACCCCTGGCCATACCCTCTGCTGCTGCTGCTGCTGCTGCAGCCGGCCGCATTGCCAT CCCAGGGCTGGCAGGCGCCGGGAATTCCGTCCTTTTGGTCAGCAACTTGAACCCTGAGAGAGTCACACCC CAAAGCCTCTTTATTCTCTTCGGCGTCTACGGTGATGTGCAGCGGGTGAAGATCCTGTTCAACAAGAAGG AGAACGCACTTGTGCAGATGGCAGACGGCAGCCAGGCCCAGCTGGCCATGAGCCACCTGAACGGGCACAA GCTGCATGGGAAGTCAGTGCGCATCACCCTGTCAAAGCACCAGAGTGTGCAGCTGCCTCGTGAGGGACAG GAGGACCAGGGCCTGACCAAGGACTATGGCAGCTCACCGCTGCACCGCTTCAAGAAACCTGGCTCCAAGA ACTTTCAGAACATCTTTCCTCCCTCAGCTACCCTGCACCTCTCCAACATCCCGCCCTCTGTGTCAGAGGA TGACCTCAAGAGCCTCTTCTCCAGCAATGGCGGTGTGGTCAAAGGCTTCAAGTTCTTCCAGAAGGACCGC AAGATGGCACTGATCCAGATGGGCTCTGTGGAGGAGGCAGTGCAGGCACTAATTGAGCTGCACAACCATG ACCTGGGCGAGAACCACCACCTGCGAGTGTCCTTTTCCAAGTCCACCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022516 |
| Insert Size | 1593 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022516.5, NP_071961.2 |
| RefSeq Size | 2827 bp |
| RefSeq ORF | 1593 bp |
| Locus ID | 29497 |
| Gene Summary | DNA binding protein; involved in the negative regulation of alternative splicing [RGD, Feb 2006] Transcript Variant: This variant (2) lacks a coding exon compared to variant 1. The resulting protein (isoform b) is shorter but has the same N- and C-termini compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR214589 | Ptbp1 (Myc-DDK-tagged ORF) - Rat polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, (10 ug) |
CNY 5,920.00 |
|
| RR214589L3 | Lenti ORF clone of Ptbp1 (Myc-DDK-tagged ORF) - Rat polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, (10 ug) |
CNY 8,740.00 |
|
| RR214589L4 | Lenti ORF clone of Ptbp1 (mGFP-tagged ORF) - Rat polypyrimidine tract binding protein 1 (Ptbp1), transcript variant 2, (10 ug) |
CNY 9,600.00 |
