Col5a3 (NM_021760) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214167 representing NM_021760 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGCTGCCGGAGACTGGACCAGCTTCAGGCCGGCCTCTGCCTGCTCCTGGCCTCCCTACAACTCG TGTCCTGGACGCTGGCTGCAGAACCTGTGGATGTATTGGAAACCTTGGGTGTGCATAGGGACAAGGCTGG GGTGACCAAAGGGCCTGGCTTCTGCCCCCAGAGGATTCCACAGGGTGACCGAGCATTCAGGGTGGGCAAG TCCAGCCTTCTCAGCGTCCCCACATGGCAGCTCTTTCCAGATGGACATTTTCCTGAGAACTTTTCTGTGC TGCTCACTCTGCGGGCCCAGCCAGCCAATCAATCTGTCCTTCTGTCTATATATGACGAGAATGGTGTCCG GCAGATGGGGCTGGCACTGGGGCCAGCTCTGGGTCTCCTTGGTGACTCTTTCAGGTCTCTTCCCAAGCAG ATCAACCTTATGGATGGCAGGTGGCACCGGGTGGCGGTCAGCATCAGCGGTGACAAGGTGACCCTGGTGG TTGACTGTGAACCTCAGCCCCCCATGTTTGGTCAGGGGCCTCGGGTTATAAGTACAGCTGGACTCACGGT GATGGGAACCCAGGAGCTCGGGGAGGAGTCTTTTGAGGGAGACATCCAGGAGCTGCTGTTAATTCCAGAC CCTCAGGCTGCTCTCCAGGCCTGTGAGCTCTACCTGCCTGACTGTGAGACCCCCGATTCCACAACCACAG GGGTCACTAAAGAAGAACCAGAAACACCTGCCCCTCGTCGACGAAAGGGCAAAGGAAAGAAAAAAGATCG GGGTCGCAAGGGCAAGGGAAGAAAGAAAAACAAGACCTCAAGACTGAGTCCAACCCCAGGTGCCCCTGAG AACCAGACCTCCCTCCACACCCCTGAGACAGAGAAGCCATCGCCCCATCTGCCTCCAACTCCCACACCTT TGGCCATCACCACCACTGTCACGATGGGAGGAAATGCCACAGTTTTGCAGGGGTTGGACTCCAGTACTGA AACTGAGCAGATGACTCCAGAGATGGACGCTACTGAAGAGAGTGAAGGAGGTGGCCCGACCATGGGCCCC GAGTTCCGGGCAGCAGAACAGTCTTTACAGACTGAGTTCCAGATCTTTCCTGGTGCTGGAGAAAAGGGAG CGAAAGGAGAACCTGCGACTATAGAGCAGGGACAGCAGTTTGAGGGACCTGCAGGAGCTCCAGGACCCCG GGGAATATCTGGTCCTTCAGGCCCTCCTGGGCCTCCGGGCTTCCCTGGGGACCGTGGCCTACCGGGCCCT GCTGGCCTCCCAGGAATCCCAGGCATTGATGGCGTCCGGGGCCTGCCAGGCACAGTGATCATGATGCCGT TCCATTTTGCAAGCGGCTCGATGAAAGGACCCCCAGTCTCCTTTCAACAGGCACAGGCCCAGGCAGTACT GCAACAGGCTCAGCTGTCCATGAAAGGGCCCCCTGGTCCAGTAGGGCTCACTGGGCGCCCAGGCCCTGTG GGCCTCCCTGGATATCCAGGTCTGAAAGGTGAATTGGGAGAAGCAGGGCCTCAGGGCCCCCGAGGATTGC AGGGCCCTCCTGGGCCTCCTGGACGGGAAGGCAAGACAGGCCGAGCTGGAGCAGATGGGGCTCGGGGTCT TCCGGGAGACACAGGACCTAAGGGTGACCGGGGCTTTGATGGCCTGCCTGGACTGCCTGGTGAGAAGGGC CAGAGGGGTGACTTTGGACCTGTAGGACAACCTGGTCCCCCAGGAGAAGATGGTATAAAGGGCCTGCAGG GACCTCCAGGCCCCACTGGCCAGGCTGGAGAGCCGGGTCCCCGAGGTCTGATTGGCCCCAGAGGCCCCCC TGGTCCTTTAGGACGCCCGGGTGTGACAGGGAGTGATGGCGCACCGGGTGCCAAAGGCAACGTGGGTCCT CCCGGAGAACCAGGCCCCCCAGGACAGCAAGGAAACCACGGCTCCCAGGGAATCCCAGGCCCCCAGGGGC CCATTGGCACACCCGGGGAGAAGGGTCCTCCTGGAAACCCCGGAATTCCAGGTGTCCCAGGATCTGAGGG CCCCCCGGGCCACCCAGGCCATGAGGGTCCCACCGGAGAAAAGGGGGCTCAGGGCCCACCAGGGTCAGCA GGCCCTCAGGGCTATCCTGGACCTCGTGGTGTGAAGGGTACCTCTGGTAACCGGGGCCTCCAAGGAGAGA AAGGAGAAAGGGGAGAGGATGGTTTTCCAGGCTTCAAGGGTGATGAGGGACCAAAAGGCGACCGGGGAAA CCCGGGACTCCCAGGTCCCAGAGGAGAGGATGGCCCAGAAGGACAAAAGGGGCCTGAGGGACTGCCTGGT GATGAGGGCCCCCCAGGAGCAGCAGGGGAGAAGGGCAAGCTTGGGGTGCCAGGTCTCCCAGGTTATCCAG GACGCCCAGGACCTAAGGGATCTATTGGATTTCCTGGGCCCCTTGGACCACTGGGGGAGAAAGGCAAGCG GGGCAAAGCAGGGCAGCCAGGGGAGGAAGGAGAACGTGGCACACCGGGCACCAGAGGAGACAGAGGACAG CCAGGGGCCACAGGCCAGCCTGGCCCTAAGGGAGATGTGGGCCAGAACGGGTCTCCTGGGGCCCCCGGAG AAAAGGGTCTACCCGGTCTGCAAGGTCCCCCAGGATTCCCTGGGCCAAAAGGCCCCCCAGGTCCTCAGGG GAAAGATGGGATACCCGGACACCCTGGACAAAGAGGAGAATTGGGCTTCCAAGGTCAGACAGGCCCCCCT GGACCAGCTGGTGTCCTTGGTCCTCAGGGAAAGGTAGGGGATGTGGGTCCTCTAGGCGAGAGAGGCCCCC CAGGGCCTCCTGGACCGCCTGGTGAACCAGGTCTGCCAGGCATAGAAGGCAGAGAAGGGGCCAAGGGTGA GCTAGGACCCCTGGGGTCCCTCGGAAAGGAGGGGCCACCTGGGCCCATGGGCTTCCCTGGCCCACAAGGA GCCCCCGGAGACCCAGGACCCATTGGTTTGAAGGGTGACAAGGGTCCCCCGGGCCCTCTTGGGGCAAATG GCTCCCCAGGTGAGCGTGGTCCTGTGGGCCCCTCTGGAGGCATTGGGCTTCCTGGCCAGAGTGGTGGGCA AGGCCCTATTGGTCCTGCTGGTGAGAAGGGGTCCCCGGGAGAACGGGGTGTGCCTGGTCCTACTGGCAAA GATGGTATTCCAGGGCCCCCGGGACTTCAGGGGCCCCCTGGAGCTGCAGGGCCTTCTGGTGAGGAAGGAG ACAAGGGGGAAGTAGGGATGCCTGGACACAAGGGAAGCAAAGGGGATAAAGGAGATGCGGGCCCACCTGG ACCAACAGGAATAAGGGGTCCAGCGGGCCATCCAGGCCCCCCGGGTGCTGATGGTGCTCAGGGTCGCCGG GGACCCCCTGGCCTCTTTGGGCAGAAAGGTGATGATGGAGTCCGAGGCTTTGTAGGTGTAATTGGTCCTC CAGGCCTGCAGGGGCTGCCGGGCCCTCCTGGGGAGAAAGGCGAGGTTGGAGACGTGGGATCCATGGGTCC CCACGGAGCTCCAGGCCCTCGGGGTCCCCCTGGGCCCAGTGGATCAGCGGGTTCCCCAGGGCTGCCTGGA GGAGTAGGGCAGCCTGGCGCTGTGGGCGAGAAGGGTGAGCCAGGGAATGCTGGAGACGCTGGACCCCCAG GAGTTCCAGGCATCCCTGGGCCCAAAGGTGAAATTGGTGAAAAGGGGGATTCGGGTCCATCAGGGGCTGC TGGTCCTCCAGGCAAAAAGGGACCTCCTGGAGAGGACGGAGCTAAGGGGAACATGGGTCCCACAGGACTC CCTGGAGATCTAGGACCCCCAGGAGACCCTGGAGTTCCGGGTACGGATGGCATCCCAGGGGAGAAGGGGA ATGCTGGTGATATTGGGGGACCGGGGCCACCTGGAGCTTCAGGGGAACCTGGTGCCCGTGGGCTCCCTGG CAAGAGGGGTTCCCCTGGCCGCATGGGTCCAGAAGGAAAAGAGGGAGAGAAAGGCGCCAAGGGAGATGCT GGTCCCGATGGCCCCCCAGGCAGAACAGGCCCCATTGGGGCTCGGGGGCCCCCTGGACGGGTTGGGCCTG ATGGTCTTCCAGGGATCCCTGGTCCTGTGGGTGAACCAGGTCTCCTGGGACCTCCTGGGCTAATCGGCCC TCCAGGGCCCCTGGGCCCACCTGGCCTCCCTGGCCTGAAGGGAGACGCTGGCCTCAAGGGGGAAAAGGGC CACATTGGGCTAATCGGCCTCATTGGTCCCCCAGGGGAGGCTGGGGAAAAAGGCGATCAGGGGTTGCCAG GTGTGCAGGGCCCTCCAGGCCTTCAGGGAGACCCTGGTCTTCCTGGTCCTGTTGGCTCCTTAGGTCACCC TGGGCCCCCAGGTGTGGTGGGCCCTCTGGGACAGAAGGGTTCCAAGGGGTCCCCGGGATCTCTTGGACCT CGTGGAGACCCTGGACCAGCAGGTCCTCCTGGTCCCCCGGGTTCCCCTGCTGAGGTGCACGGCCTGCGTA GGCGCCGCTCTGTGTTGGACACCCCAGAAGGTGGCCTGGAGGAAGTGATGGCCTCACTCAATTCACTGAG CTTGGAGCTGGAGCAGTTGCAGCGACCCCCAGGCACAGCCGAGAGCCCAGGCCTCATATGCCACGAGCTT CATCGAAACCACCCACACCTGCCGGACGGAGAGTACTGGATTGACCCCAATCAGGGCTGTGCACGGGACG CCTTCAAGGTCTTCTGCAACTTCACTGCCGGAGGTGAGACCTGTCTCTACCCAGACAAGAAGTTTGAGAC GGTGAAACTGGCCTCCTGGTCCCGAGAAAAGCCTGGAGGTTGGTACAGCACCTTCCGCCGAGGGAAGAAG TTCTCCTATGTGGATGCTGACGGCTCCCCGGTGAATGTGGTCCAGTTGACCTTCCTGAAGTTGTTGAGCG CTGCAGCCCATCAGAGGTTCACTTACACCTGCCAGAACTCCGCAGCATGGCTGGACGAAGCTTCAGGTGA CCACAGGCACTCTATCCGCTTCCAAGGGACCAACTGGGAAGAGTTGTCCTTCAACCAGACAACAGCAGCC ACCATCAAGGTCCCCCATGATGGCTGCCGGGTCCGGAAGGGACAGGCGAAGACCCTCTTTGAATTCAGCT CTTCTGTGGGCTTTCTGCCTCTGTGGGATGTGGCCGCCGTTGACTTTGGCCAGACGAACCAGAAGTTTGG GTTTGAACTTGGCTCCGTCTGCTTTAGCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021760
Insert Size	5214 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021760.1, NP_068528.1
RefSeq Size	6076 bp
RefSeq ORF	5214 bp
Locus ID	60379
Gene Summary	alpha chain of type V collagen [RGD, Feb 2006]

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