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Mief1 (NM_001007709) Rat Untagged Clone
CAT＃: RN213849
Smcr7l (untagged ORF) - Rat Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l), (10 ug)
CN¥ 5,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Smcr7l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213849 representing NM_001007709
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCGCTGGTGAGCGCAAAGGCAAGAAGGATGACAATGGCATTGGAACGGCCATTGATTTTGTGC TCTCCAATGCCCGGCTGGTGCTGGGCGTGGGTGGAGCAGCCATGCTGGGCATCGCCACATTGGCAGTTAA GCGGATGTACGACCGGGCAATTAGTGCCCCTACCAGCCCCACCCGCCTAAGCCATTCAGGAAAGAGGAGC TGGGAGGAACCAAACTGGATGGGCTCTCCTCGACTATTGAACAGGGACATGAAGACAGGTCTGAGCCGGT CCCTGCAGACCCTTCCCACAGACTCTTCTGCCTTTGACACAGATACATTCTGCCCACCCCGGCCCAAACC TTTGGCCAGGAGGGGCCAGGTAGACTTGAAGAAGTCACGACTCCGCATGTCCCTGCAGGAGAAACTTCTT TCTTACTACCGGAACCGTGCAGCCATCCCTGCTGGAGAGCAGGCTCGAGCCAAGCAAGCTGCGGTGGACA TATGTGCTGAGCTGCGGAGCTTCCTGCGGGCCAAGCTGCCTGACATGCCACTCCGGGACATGTACTTGAG TGGCAGCCTCTATGACGATCTGCAGGTGGTGACAGCTGACCATATCCAACTCATTGTCCCCCTCGTGCTG GAGCAGAACCTGTGGTCGTGCATACCTGGGGAGGACACCATCATGAATATCCCTGGTTTCTTCCTGGTCC GTCGTGAGAACCCAGAGTACTTTCCTCGTGGTAGCAGTTATTGGGACCGCTGTGTTGTAGGTGGCTACCT CTCCCCAAAGACAGTGGCAGACACCTTTGAGAAGGTGGTGGCAGGCTCCATCAACTGGCCAGCCATAGGG TCCCTCTTAGACTATGTGATCCGACCAGCACCACCCCCAGAGGCCCTGACACTAGAAGTGCAGTATGAGA GGGACAAACATCTTGTCATTGACTTCCTGCCATCAGTGACCCTTGGTGACACTGTCTTGGTGGCCAGACC ACACCGGTTAGCTCAGTATGACAACCTGTGGCGGCTGAGCCTTCGTCCTGCTGAGACGGCACGCTTACGG GCTTTGGACCAGGCGGACTCCGGCTGCCGGTCTCTGTGCCTCAAGATCCTCAAGGCCATATGCAAGTCCA CTCCAGCTCTGGGCCACCTCACTGCCAGCCAGCTAACCAATGTCATCCTCCACTTGTCCCAGGAGGAGGC TGACTGGTCTCCAGACATGTTGGCCGACCGCTTTCTACAGGCCCTGAGGGGACTCATCAGCTACTTGGAG GCTGGAGTCTTGCCCAGTGCCCTGAACCCCAAGGTGAACCTATTTGCAGAGCTCACCCCTCACGAGATAG ATGAATTGGGGTACACCCTCTACTGCTCGTTGTCTGAGCCGGAGGTGCTGCTGCAGACGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001007709 |
| Insert Size | 1392 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001007709.1, NP_001007710.1 |
| RefSeq Size | 2150 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 315141 |
| UniProt ID | Q5XIS8 |
| Gene Summary | Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213849 | Smcr7l (Myc-DDK-tagged ORF) - Rat Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l), (10 ug) |
CN¥ 5,990.00 |
|
| RR213849L3 | Lenti ORF clone of Smcr7l (Myc-DDK-tagged ORF) - Rat Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l), (10 ug) |
CN¥ 7,790.00 |
|
| RR213849L4 | Lenti ORF clone of Smcr7l (mGFP-tagged ORF) - Rat Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l), (10 ug) |
CN¥ 8,650.00 |
