Nfatc4 (NM_001107264) Rat Untagged Clone

CAT#: RN213834

Nfatc4 (untagged ORF) - Rat nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), (10 ug)



  "NM_001107264" in other vectors (3)

CNY 11,590.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN213834 representing NM_001107264
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCGGCAAGCTGCGAGGATGAGGAGCTGGAATTTAAGCTGGTGTTCGGGGAGGAAAAGGAGGCCC
CCCCGCTGGGCCCTGGGGGCCCTGGGGAAGAGCTGGACTCGGAGGATGCCCCTCCGTGCTGTCGTTTGGC
CCTGGGGGAACCCCTTCCTTATGGTGCTGCCCCTATTGGTATTCCCCGACCCCCACCCCCTCGGCCAGGG
ATGCACTCACCACCACCCCGTCCTGCCCCTTCACCTGGCACCTGGGAGAGCCAGCCACCTCGGTCGGTGA
GGCTGGGGGGCCCAGGAGGCACTGCGGGGGGTACTGGGGGCGGTCGTGTTCTTGAGTGTCCCAGCATCCG
GATCACCTCTATCTCTCCCACGCCTGACCCACCGACCTCACTAGAGGATGCTCCTGAAACTTGGGGGGAC
GGCTCTCCTAGAGATTACCCTCCACCAGAAGGCTTTGGGGGCTACCGAGAGGCTGGAGGTCAGGGCGGAG
GAGCCTTCTTCAGCCCGAGCCCTGGCAGCAGCAGCCTGTCTTCGTGGAGCTTCTTCTCTGATGCCTCAGA
CGAGGCAGCCCTGTATGCAGCATGCGACGAAGTGGAGTCTGAACTTAATGAAGCAGCCTCTCGTTTCGGC
TTGAGCTCTCCACTGCCCTCGCCCCGGGCTTCTCCTAGGCCATGGACCCCGGAGGATCCGTGGAGCCTAT
ATGGTCCAAGCTCCGGAGGCCGAGCGCCAGAGGATAGCTGGCTACTTCTCAGCGCTCCTGGGCCCATCCC
AGCCTCCCCTCGGCCTGCTTCTCCCTGCGGCAAGCGGCGCTATTCCAGTTCCGGAACCCCATCTTCGGCC
TCCCCAGCTTTGTCCCGCCGGGGTAGCCTTGGGGAAGAGGGTCCAGAGCCACCTCCACCACCCCCATTGC
CTCTGGTCCGGGACCCTAGTTCCTCTGGCCCTTTTGACTATGTGGGTGCTCCACCAACCGAGAGCGTCCC
TCAGAAAACCAGGAGGACTTCTAGCGAGCAAGCAGTGGCCTTGCCTCGGTCTGAGGAGCCTGCCTCGTGC
AATGGGAAGCTGCCCTCTGGTACAGAGGACTCCGTGGCTGCTCCCGGGGCTCTGCGGAAAGAGATGGCTG
GCATGGATTACTTAGCAGTTCCTTCTCCCCTCGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCCAT
CTTCAGGACCTCTGCCCTACCTCCCCTGGACTGGCCTTTACCCAGCCAGTACGAGCAGCTGGAGCTGAGG
ATTGAGGTGCAGCCCAGAGCTCACCACCGAGCTCATTATGAGACTGAGGGCAGCCGGGGAGCTGTCAAAG
CTGCTCCTGGAGGTCACCCTGTGGTCAAGCTCCTAGGCTACAATGAGAAGCCATTGACTCTTCAGATGTT
CATTGGCACTGCAGACGAGAGGAGCCTGCGGCCCCATGCCTTCTACCAGGTGCACCGGATCACTGGCAAG
ATGGTGGCTACAGCCAGCTATGAAGCTGTAGTCAGTGGTACTAAAGTGTTGGAGATGACTTTGCTCCCAG
AGAACAACATGGCTGCCAACATTGACTGTGCTGGAATCCTGAAGCTTCGGAATTCAGACATTGAGCTACG
GAAGGGCGAGACAGACATCGGGCGCAAAAACACACGTGTGCGACTGGTGTTCCGTGTGCATGTGCCTCAG
GGCGGCGGGAAGGTCGTCTCTGTGCAGGCAGCATCGGTGCCCATCGAGTGCTCCCAGCGCTCAGCCCAGG
AGCTGCCCCAAGTGGAGGCCTACAGCCCCAGTGCCTGCTCCGTGAGAGGAGGGGAGGAGCTAGTGTTGAC
TGGATCCAACTTCCTGCCAGACTCTAAAGTGGTGTTCATTGAGAGGGGCCCTGATGGAAAACTGCAGTGG
GAGGAAGAGGCCGCAGTGAACCGGCTGCAGAGCAGTGAGGTGACACTGACTCTGACCATCCCTGAGTACA
GCAACAAGCGGGTGTCCCGGCCAGTCCAGGTCTACTTTTATGTCTCCAATGGGCGGAGGAAGCGCAGTCC
CACCCAAAGTTTCAAGTTCCTGCCTGTGATCTTCAAGGAGGAGCCCCTACCAGACTCATCTCTCCGGGGC
TTCCCTTCCACATCGGGTCCCCCCTTTGGCCCCGACATGGACTTCTCACCGCCCAGGCCCCCTTATCCCT
CCTATCCCCATGAAGACCCTGCTTATGAGACTCCTTATCTGTCAGAAGGCTTTGGTTATAGCACACCCGC
TCTGTACCCCCAGACAGGGCCCCCACCGTCTTATAGATCTGGCCTACGGATGTTCCCTGAGACTGGGGGT
ACCACAGGTTGTGCCCGCCTACCTTCAGTCTCCTTTCTTCCGCGCCCCTTTCCTGGTGATCAGTATGGAG
GACAGGGCTCCTCTTTTCCCCTGGGGCTTCCATTCTCCCCTCCGGCCCCCTTTAGGCCTCCATTACCTTC
CTCCCCACCACTCGAAGACCCCTTTAACCCCCAGAGTGCTGTCCACCCCTTACCTGCTGAGGGCTACAAT
GAGGTGGGGCCAGGCTATACCCCTGGGGAGGGGGCTTCGGAGCAGGAGAAATCCAGGGGTGGCTACGGCA
GCGGCTTCAGAGACAATGTGCCTATCCAGGGTATCACCCTGGAGGAAGTGAGTGAGATCATTGGCCGAGA
CCTGAGTGGCTTCCCTGCACGTCCTGGAGAAGAGCCTCCTGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107264
Insert Size 2706 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107264.1, NP_001100734.1
RefSeq Size 3170 bp
RefSeq ORF 2706 bp
Locus ID 305897
UniProt ID D3Z9H7
Gene Summary Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed:12370307). Along with NFATC3, involved in embryonic heart development. Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function. Transactivates many genes involved in heart physiology. Along with GATA4, binds to and activates NPPB/BNP promoter (PubMed:9568714). Activates NPPA/ANP/ANF and MYH7/beta-MHC transcription (PubMed:19026640). Binds to and transactivates AGTR2 gene promoter. Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation. In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during a developmental critical period when auditory neurons depend on afferent input for survival (By similarity). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP). Plays a role in adipocyte differentiation. May be involved in myoblast differentiation into myotubes (By similarity). Binds the consensus DNA sequence 5'-GGAAAAT-3' (By similarity). In the presence of CREBBP, activates TNF transcription. Binds to PPARG gene promoter and regulates its activity (By similarity). Binds to PPARG and REG3G gene promoters (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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