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Fads2 (NM_031344) Rat Untagged Clone
CAT＃: RN213635
Fads2 (untagged ORF) - Rat fatty acid desaturase 2 (Fads2), (10 ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Fadsd6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213635 representing NM_031344
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGGAGGTAACCAGGGAGAGGGGAGCACCGAGCTCCAGGCTCCGATGCCCACCTTCCGCTGGG AGGAGATTCAGAAGCACAACCTGCGCACCGACCGGTGGCTCGTCATCGACCGCAAGGTCTACAACGTTAC CAAATGGTCCCAGCGGCACCCAGGGGGGCACCGTGTCATCGGACACTATTCGGGAGAAGATGCTACGGAT GCCTTCCGTGCCTTCCACCTGGACCTGGATTTCGTGGGCAAGTTCTTGAAGCCCCTGCTGATTGGTGAGC TGGCCCCAGAGGAGCCCAGCCTGGACCGGGGCAAAAGCTCTCAGATCACCGAGGACTTCAGGGCCCTGAA GAAGACTGCTGAGGACATGAACCTTTTCAAAACCAACCACCTGTTCTTCTTTCTCCTCCTGTCCCACATC ATCGTCATGGAAAGCATCGCCTGGTTCATCCTCTCGTACTTCGGCAATGGCTGGATTCCCACCGTCATCA CGGCCTTTGTCCTTGCTACCTCCCAGGCCCAAGCTGGATGGCTACAACATGATTATGGCCACCTTTCTGT CTATAAGAAATCCATATGGAACCACATTGTCCACAAGTTTGTCATTGGCCACTTAAAGGGTGCCTCCGCC AACTGGTGGAACCATCGACATTTCCAGCACCATGCGAAGCCCAACATCTTCCACAAGGACCCCGACATAA AGAGCCTGCACGTGTTTGTCCTTGGAGAGTGGCAGCCCCTCGAGTATGGCAAGAAGAAGCTGAAATATCT GCCCTACAACCACCAGCATGAATACTTCTTCCTGATCGGACCACCGCTGCTCATCCCTATGTACTTCCAG TACCAGATCATCATGACCATGATCAGACGCAGAGACTGGGTGGACTTGGCCTGGGCCATCAGCTACTATG CACGTTTCTTCTACACCTATATCCCTTTCTATGGCATCTTGGGAGCCCTGGTTTTCCTCAACTTTATCAG GTTCCTGGAGAGCCACTGGTTTGTGTGGGTCACACAGATGAACCACATTGTCATGGAGATTGATCTTGAT CACTACCGGGACTGGTTCAGCAGCCAGCTGGCAGCCACCTGCAATGTGGAGCAGTCCTTCTTCAATGACT GGTTCAGCGGGCACCTCAATTTCCAGATTGAGCACCACCTCTTCCCCACTATGCCAAGACACAACTTGCA CAAGATTGCCCCACTGGTGAAGTCTCTCTGCGCCAAGCATGGCATTGAATACCAAGAGAAGCCGCTGCTG AGGGCCCTGCTCGACATTGTGAGTTCACTGAAGAAGTCTGGGGAGCTGTGGCTGGATGCCTACCTCCACA AATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_031344 |
| Insert Size | 1335 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031344.2, NP_112634.1 |
| RefSeq Size | 1706 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 83512 |
| UniProt ID | Q9Z122 |
| Gene Summary | Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:10049752, PubMed:14563830, PubMed:22216341, PubMed:11988075). Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (PubMed:11988075). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (PubMed:24070791).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213635 | Fads2 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 2 (Fads2), (10 ug) |
CNY 5,700.00 |
|
| RR213635L3 | Lenti ORF clone of Fads2 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 2 (Fads2), (10 ug) |
CNY 7,600.00 |
|
| RR213635L4 | Lenti ORF clone of Fads2 (mGFP-tagged ORF) - Rat fatty acid desaturase 2 (Fads2), (10 ug) |
CNY 8,360.00 |
