Acp2 (NM_016988) Rat Untagged Clone
CAT#: RN213595
Acp2 (untagged ORF) - Rat acid phosphatase 2, lysosomal (Acp2), (10 ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | LAP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN213595 representing NM_016988
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCAGACAGTCTGGTTGGAGCCAGGCGGCTCTTCTCCAGTTCCTTCTTGGCATGTGCCTAATGG TGATGCCACCCATACAAGCCCGGAGTCTGCGCTTTGTTACCTTGCTGTATCGACACGGAGATCGGTCACC AGTGAAGGCATATCCTAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAACCAAG GAAGGGATGCTACAGCATTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAACGCCT CTTACCACAGGCAAGAGGTTTACGTGCGAAGCACAGACTTTGACCGTACTCTCATGAGTGCAGAGGCCAA CCTGGCCGGACTCTTCCCTCCCACTGAAGTTCAGCACTTCAACCCGAACATTTCATGGCAGCCTATCCCT GTCCACACCGTGCCCATTACTGAAGACAGGTTGCTGAAGTTTCCTTTGGGTCCATGTCCCCGTTATGAGC AGTTGCAGAACGAGACTCGGCAGACACCAGAGTATCAGAACATGAGTATTCAGAATGCACAATTTCTGGA CATGGTGGCCAATGAGACAGGGCTTATGAACTTGACCCTAGAGACCATCTGGAATGTGTATGACACACTC TTTTGTGAGCAAACACATGGGCTGCTCCTGCCACCCTGGGCCTCTCCCCAAACCGTGCAGCGTCTGAGCC AGCTAAAGGACTTCAGCTTCCTCTTCCTCTTCGGGATCCACGATCAAGTACAGAAGGCCCGGCTTCAGGG GGGAGTTCTGCTGGCTCAAATATTGAAGAATCTGACCCTAATGGCAACTACCTCTCAATTCCCTAAGCTT CTGGTTTATTCTGCGCATGACACTACCCTGGTTGCTCTGCAAATGGCACTGAATGTCTACAATGGTAAAC AAGCCCCCTATGCTTCCTGCCACATATTTGAACTGTACCAGGAAGATAATGGGAATTTCTCAGTCGAGAT GTACTTTCGGAATGACAGTAAGAAGGCACCCTGGCCACTGACCCTGCCTGGCTGTCCTCACCGTTGCCCA CTGCAGGACTTCCTTCGCCTCACAGAACCTGTCATACCCAAGGACTGGCAGAAGGAGTGCCAGCTAGCAA GCGATACTGCAGACACAGAGGTGATTGTGGCACTGGCTGTCTGTGGCTCCATCCTCTTCCTTCTAATAGT GTTGCTCCTCACTGTCCTCTTCCGGATGCAGGCCCAGCCTCCTGGCTACCACCATGTTGCAGACAGGGAA GACCATGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_016988 |
Insert Size | 1272 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016988.2, NP_058684.2 |
RefSeq Size | 2067 bp |
RefSeq ORF | 1272 bp |
Locus ID | 24162 |
Gene Summary | The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017] Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR213595 | Acp2 (Myc-DDK-tagged ORF) - Rat acid phosphatase 2, lysosomal (Acp2), (10 ug) |
CNY 3,990.00 |
|
RR213595L3 | Lenti ORF clone of Acp2 (Myc-DDK-tagged ORF) - Rat acid phosphatase 2, lysosomal (Acp2), (10 ug) |
CNY 6,080.00 |
|
RR213595L4 | Lenti ORF clone of Acp2 (mGFP-tagged ORF) - Rat acid phosphatase 2, lysosomal (Acp2), (10 ug) |
CNY 6,650.00 |