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Usp7 (NM_001024790) Rat Untagged Clone
CAT＃: RN213504
Usp7 (untagged ORF) - Rat ubiquitin specific peptidase 7 (herpes virus-associated) (Usp7), (10 ug)
CNY 17,670.00
货期*
7周
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Hausp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213504 representing NM_001024790
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCACCAACAGCAGCAGCAGCAGCAGCAGAAAGCGGGCGAGCAGCAGCTGAGCGAGCCCGAGGACA TGGAGATGGAAGCTGGAGATACAGATGACCCACCAAGAATTACTCAGAACCCCGTCATCAATGGAAATGT AGCCCTGAGTGATGGGCACAGCAACGCAGAGGAGGACATGGAGGACGACACCAGTTGGCGCTCCGAGGCA ACCTTTCAGTTCACTGTCGAGCGCTTCAGCAGACTGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGC GAAATCTGCCATGGAAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCGCACCAAAAAAGCGTAGG ATTCTTTCTCCAGTGCAATGCTGAATCTGACTCCACGTCGTGGTCCTGCCATGCACAAGCAGTGCTGAAG ATAATAAATTACAGGGATGACGACAAGTCATTCAGCCGGCGAATCAGCCACTTGTTCTTCCACAAGGAAA ACGACTGGGGATTCTCTAATTTCATGGCCTGGAGTGAAGTGACTGATCCTGAGAAAGGATTTATAGATGA TGACAAAGTCACCTTTGAAGTTTTTGTACAGGCGGATGCTCCCCATGGAGTTGCGTGGGACTCAAAGAAG CATACTGGCTATGTCGGCTTAAAGAATCAGGGAGCGACGTGTTACATGAATAGCTTGCTGCAGACCTTGT TTTTCACAAATCAGTTACGAAAGGCTGTGTACATGATGCCAACAGAGGGTGATGATTCATCTAAAAGCGT CCCTTTAGCATTGCAGAGAGTGTTCTATGAGTTACAGCACAGCGATAAGCCTGTAGGAACAAAAAAGCTC ACAAAGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACACGATGTTCAAGAACTTTGTCGAGTGT TGCTTGATAATGTGGAAAATAAAATGAAAGGCACATGTGTAGAAGGCACCATTCCCAAGTTATTCCGAGG CAAAATGGTGTCTTATATTCAGTGCAAAGAAGTAGACTACCGTTCTGATAGGAGAGAAGATTATTATGAT ATCCAGCTAAGTATAAAAGGAAAGAAAAACATATTTGAATCATTTGTGGATTATGTGGCAGTAGAACAAC TAGACGGTGACAATAAATACGACGCTGGGGAGCATGGCCTGCAGGAAGCAGAGAAGGGCGTGAAGTTCCT GACCTTGCCCCCAGTCTTACACCTGCAGCTCATGCGGTTCATGTACGACCCTCAGACGGACCAAAACATC AAGATCAATGACAGGTTTGAGTTTCCAGAACAGTTACCACTTGATGAATTTTTGCAAAAAACGGACCCTA AGGACCCTGCAAATTATATTCTCCATGCAGTCTTGGTTCACAGTGGAGATAATCATGGTGGACATTACGT GGTTTACCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATGATGATGTGGTATCCAGGTGTACT AAAGAAGAAGCAATCGAGCACAATTATGGGGGTCATGATGACGATCTATCTGTTCGACACTGCACAAATG CCTATATGTTAGTGTACATCAGGGAATCAAAGCTGAGTGAGGTGTTGCAAGCTGTCACCGACCATGATAT TCCTCAGCAGTTGGTGGAACGTTTGCAAGAAGAGAAAAGGATCGAGGCTCAGAAGCGGAAGGAGCGGCAG GAAGCCCATCTCTACATGCAAGTGCAGATAGTTGCAGAGGACCAGTTTTGTGGCCACCAAGGAAATGACA TGTACGACGAGGAGAAAGTGAGGTACACCGTGTTCAAGGTTCTGAAGAACTCCTCCCTGGCCGAGTTTGT TCAGAGCCTCTCGCAGACCATGGGGTTTCCACAAGATCAGATTCGATTATGGCCTATGCAAGCAAGGAGT AACGGGACAAAGCGGCCTGCAATGTTAGATAATGAAGCCGACGGCAGCAAAACAATGATTGAACTGAGTG ATAATGAAAACCCTTGGACAATATTCCTGGAAACAGTTGACCCTGAGCTGGCTGCTAGTGGAGCAACACT GCCCAAGTTTGATAAAGATCATGATGTAATGTTATTTTTGAAGATGTATGATCCTAAAACACGGAGCTTG AATTACTGTGGACACATCTACACACCCATATCCTGTAAAATACGTGACTTGCTCCCGGTTATGTGTGACA GAGCAGGATTTATCCAGGATACTAGCCTTATCCTCTATGAGGAAGTTAAGCCGAATTTAACAGAGAGAAT TCAGGACTATGATGTGTCTCTTGATAAAGCCCTCGATGAGCTGATGGATGGTGACATCATCGTGTTTCAG AAGGATGACCCGGAAAACGATAACAGTGAGCTACCCACTGCTAAGGAGTACTTCAGAGACCTCTACCATC GCGTCGACGTCATCTTCTGTGACAAAACGATCCCTAATGACCCTGGGTTTGTGGTCACCTTATCAAATAG GATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACACAGACCCCATGTTGCTTCAGTTT TTCAAGTCTCAAGGTTATAGGGACGGCCCCGGTAATCCCCTTAGGCATAATTACGAAGGTACTTTAAGAG ATCTTCTACAATTCTTCAAGCCAAGACAACCTAAGAAACTTTACTATCAGCAGCTTAAGATGAAAATCAC GGACTTTGAGAACAGGAGAAGTTTTAAGTGTATATGGTTAAACAGCCAATTTAGGGAAGAGGAAATAACA CTATACCCAGACAAGCATGGCTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAAGCCGTGGAGCTTGGTG ACGAAGCATCAGGGAGACTTAGGCTGCTAGAAATTGTGAGCTACAAAATTATTGGTGTGCACCAAGAAGA TGAACTATTAGAGTGCTTATCTCCTGCAACGAGTCGGACTTTTAGAATAGAGGAAATACCTTTGGACCAG GTAAACATCGATAAGGAAAACGAGATGCTAATCACGGTGGCGCATTTCCACAAAGAAGTGTTTGGGACAT TCGGAATTCCGTTTTTGCTGAGGATACACCAGGGTGAGCATTTCAGAGAAGTGATGAAGCGGATTCAGAG CCTGCTGGATATCCAGGAAAAGGAGTTTGAAAAGTTTAAATTCGCAATTGTCATGATGGGCCGGCACCAG TACATCAATGAAGATGAGTATGAAGTGAATTTGAAGGACTTTGAGCCACAACCTGGTAACATGTCTCATC CTCGGCCTTGGCTAGGGCTTGACCACTTCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTAGAAAA GGCAATTAAAATCCACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024790 |
| Insert Size | 3312 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024790.1, NP_001019961.1 |
| RefSeq Size | 3312 bp |
| RefSeq ORF | 3312 bp |
| Locus ID | 360471 |
| UniProt ID | Q4VSI4 |
| Gene Summary | Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX (PubMed:16111684, PubMed:16328052). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation (By similarity). Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (By similarity). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis (By similarity). Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML (By similarity). Deubiquitinates KMT2E preventing KMT2E proteasomal-mediated degradation (By similarity). Involved in cell proliferation during early embryonic development (By similarity). Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6 (By similarity). Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (By similarity). Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions (By similarity). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (By similarity). Able to mediate deubiquitination of histone H2B; it is however unsure whether this activity takes place in vivo (By similarity). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing transcription factor FOXP3 which is crucial for Treg cell function (By similarity). Plays a role in the maintenance of the circadian clock periodicity via deubiquitination and stabilization of the CRY1 and CRY2 proteins (By similarity). Deubiquitinates REST, thereby stabilizing REST and promoting the maintenance of neural progenitor cells (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213504 | Usp7 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 7 (herpes virus-associated) (Usp7), (10 ug) |
CNY 17,670.00 |
|
| RR213504L3 | Lenti ORF clone of Usp7 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 7 (herpes virus-associated) (Usp7), (10 ug) |
CNY 19,290.00 |
|
| RR213504L4 | Lenti ORF clone of Usp7 (mGFP-tagged ORF) - Rat ubiquitin specific peptidase 7 (herpes virus-associated) (Usp7), (10 ug) |
CNY 21,280.00 |
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