Tg (NM_030988) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Tgn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213478 representing NM_030988 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACCTTGGTCTTGTGGGTCTCGACTTTGTTGAGCTCAGTCTGCCTGGTAGCAGCCAACATCTTTG AGTACCAAGTGGATGCACAGCCACTCCGCCCCTGTGAGCTGCAAAGGGAGAAGGCCTTTCTGAAGCAGGA TGAATATGTTCCACAGTGCTCTGAAGATGGAAGTTTCCAGACAGTTCAGTGCCAAAATGATGGCCAATCT TGCTGGTGTGTGGATTCTGATGGCACGGAAGTACCTGGCAGCAGGCAGCTGGGAAGGCCAACAGCATGTC TGTCCTTCTGCCAGCTACACAAGCAACGAATCCTGCTGAGCAGCTACATTAACAGCACAGATGCTCTCTA CCTTCCTCAGTGCCAGGATTCAGGGAACTATGCACCAGTGCAGTGCGACCTGCAGCAAGTGCAATGCTGG TGTGTAGACACAGAGGGGATGGAGGTGTATGGCACCCGCCAGCAGGGGAGGCCAACACGGTGTCCAAGGA GCTGTGAGATAAGAAGCCGCCGTCTCCTCCATGGAGTAGGAGACAAATCGCCACCCCAGTGCGATGCAGA TGGGGAGTTTATGCCTGTCCAGTGCAAGTTTGTCAACACCACAGACATGATGATTTTTGATCTGATTCAC AACTACAACAGGTTTCCAGATGCATTTGTGACCTTCAGTGCCTTCCGGAACAGGTTCCCTGAAGTGTCTG GGTACTGTTACTGTGCTGACAGCCAGGGCCGAGAGCTGGCTGAGACAGGACTGGAGTTGTTACTGGATGA AATTTATGACACCATTTTTGCTGGCTTGGACCAGGCCTCCACCTTCACTCAAAGCACCATGTACCGGATA TTGCAGAGACGATTCCTGGCCATTCAATTAGTCATCTCTGGCAGATTCCGATGTCCCACCAAGTGTGAAG TGGAACAGTTTACTGCCACCAGCTTTGGTCATCCATATATCCCAAGCTGCCACAGAGATGGGCACTACCA AACAGTGCAGTGCCAGATGGAAAGGATGTGCTGGTGTGTGGATGCTCAGGGAATAGAGATCCCTGGCACT CGGCAGCAAGGGCAGCCATTGTTTTGCGCTAAAGATCAATCGTGTGCCTCAGAAAGGCAGCAGGCTTTGT CTAGGCTCTACTTTGAAACCCCAGGCTACTTCAGTCCTCAGGACCTGTTGTCTTCTGAGGACAGGCTGGT CCCTGTGAGTGGTGCCAGATTGGATATATCCTGCCCACCCAGAATCAAGGAACTCTTTGTCGACTCTGGA CTCCTACGTTCTATTGCTGTGGAGCGCTACCAACAACTCTCTGAATCCAGAAGTCTACTTAGAGAAGCCA TCAGAGCAATTTTCCCCTCCCGAGAGCTGGCAGGGCTTGCCCTTCAGTTCACCACCAACCCCAAGCGACT CCAGCAAAACCTCTTTGGAGGGACATTTTTGGTGAATGCTGCCCAGCTAAACTTGTCAGGAGCCCTTGGT ACAAGAAGCACATTTAACTTCAGTCAGTTTTTCCAGCAGTTTGGACTCCCAGGCTTCCTGGTTAGAGACA GAGCTACTGATCTAGCCAAACTACTCCCTGTGAGTTTGGACTCCAGTCCTACCCCAGTACCTCTCCGTGT GCCTGAGAAGAGAGTTGCTATGAATAAGTCAGTTGTGGGTACCTTTGGCTTCAAAGTCAACCTACAAGAG AACCAAGATGCCTTAAAATTCCTTGTTTCTCTCATGGAACTTCCAGAATTTCTTGTCTTCTTGCAGCGTG CTGTTTCTGTACCTGAAGATAGAGCAAGAGATTTAGGAGATGTGATGGAAATGGTGTTCAGTGCTCAGGC CTGTAAACAGACATCTGGGAGGTTCTTTGTCCCATCATGCACTGCTGAAGGAAGCTATGAAGATATCCAG TGCTATGCTGGAGAATGCTGGTGTGTGAATTCCCAAGGCAAAGAAGTTGAAGGCTCAAGAGTCAGTGGGG GACACCCAAGATGTCCCACCAAGTGTGAGAAGCAAAGAGCTCAGATGCAAAACCTTGCAGGCGCTCAGCC TGCTGGCTCCAGTTTTTTTGTTCCTACGTGTACTAGTGAGGGATATTTTCTGCCCGTCCAGTGCTTCAAC TCAGAATGTTACTGTGTTGATGCTGAAGGCCAGGTTATTCCTGGAACTCAAAGTACAATTGGTGAACCCA AGCTGTGTCCTAGTGTCTGCCAGTTACAGGCTGAACAAGCTTTCCTTGGGGTAGTAGGGGTTCTGCTCTC CAACTCCAGTATGGTGCCTCCTATCTCCAGTGTCTACATTCCCCAATGCAGTACCAGTGGCCAGTGGATG CCTGTGCAGTGTGATGGACCCCATGAGCAGGTCTTTGAGTGGTATGAACGATGGAACACTCAGAACAGTG ATGGCCAGGAACTGACTACAGCCACACTGCTGATGAAGCTCATGAGCTACAGAGAAGTAGCTTCCACAAA CTTCAGCCTTTTTCTTCAAAGTCTCTATGATGCTGGCCAGCAAAGCATCTTCCCAGTGCTAGCACAGTAC CCATCCCTTCAGGATGTTCCACAGGTAGTGTTGGAGGGAGCCACAATTCAGCCTGGAGAGAACATTTTCC TGGATCCATACATCTTTTGGCAGATCCTAAATGGCCAGCTCAGCCAATACCCAGGACCCTACTCAGACTT TAGCATGCCTTTGGAACACTTCAACCTCAGGAGCTGCTGGTGTGTGGATGAGGCTGGTCAGGAATTGGAT GGAACTCGGACTAGGGCTGGCGAGATACCAGCATGCCCTGGCCCCTGTGAGGAAGTGAAATTTCGTGTCC TGAAATTTATCAAGGAAACAGAAGAGATCGTCTCTGCTTCCAATGCTTCTTCATTTCCACTTGGAGAGAG TTTCTTAGTAGCCAAGGGAATCCAGCTGACCAGTGAGGAGCTTGGCCTTCCTCCACTCTACCCATCTCGT GAGGCTTTCTCTGAGAAGTTTCTACGTGGGAGTGAATATGCCATTCGTTTGGCTGCTCAGTCCACCTTGA CCTTCTATCAGAAACTCCGTGCTTCCCTGGGAGAGTCCAATGGAACAGCCAGTCTTCTATGGTCAGGACC CTATATGCCACAATGCAATACTATTGGAGGCTGGGAGCCTGTGCAGTGCCATCCTGGGACAGGACAGTGT TGGTGTGTGGATGGGTGGGGAGAGCTCATCCCAGGCTCACTGATGGCACGCTCCTCCCAGATGCCCCAGT GTCCCACAAGCTGTGAGCTATCCCGAGCCAATGGGTTGATTTCTGCATGGAAACAGGCTGGACACCAAAG AAACCCAGGTCCAGGAGATCTGTTTACACCAGTCTGCCTACAGACAGGAGAGTATGTCAGACAACAGACA TCAGGAACAGGAGCTTGGTGTGTGGATCCATCTTCAGGAGAAGGGGTGCCAACAAACACAAACAGCAGTG CTCAGTGTCCTGGCCTCTGTGATGCCCTCAAGAGTAGGGTCCTGTCCAGGAAAGTCGGTCTTGGCTACAC TCCAGTCTGTGAGGCATTGGATGGGGGCTTTTCCCCAGTGCAGTGTGACTTGGCCCAGGGCAGTTGCTGG TGTGTCCTGGCAAGTGGAGAGGAAGTGCCTGGGACTCGTGTGGTAGGCACCCAGCCTGCCTGTGAAAGCC CACAGTGTCCACTGCCATTCTCTGGGTCAGATGTGACTGATGGAGTGGTCTTCTGTGAGACAGCCTCTAG CTCAGGAGTGACCACTGTTCAACAGTGCCAGCTGTTCTGCCGCCAGGGCCTCTGGAACGTCTTTTCACCA GGGCCACTGATTTGTAACCTGGAGAGTCAGCGCTGGGTGACACTGCCACTACCCAGAGCCTGTCAGAGGC CTCAGCTGTGGCAGACCATGCAAACTCAAGCACACTTCCAGCTCCTGCTCCCACCAGGCAAGATGTGCAG TATTGACTACTCTGGCTTGCTGCAGGCCTTCCAGGTCTTCATACTGGATGAGCTGATAACCCGTGGCTTC TGCCAGATCCAGGTAAAGACATTTGGGACCCTGGTTTCCAGGACTGTCTGTGACAACTCCTCAATACAGG TGGGGTGTCTGACTGCAGAGCGTTTAGGAGTAAATGCTACATGGAAGTTACAGCTTGAGGACATCTCAGT GGGCTCACTTCCAAATTTGCACAGCATTGAAAGGGCATTAATGGGCCAGGATCTCCTTGGGCGCTTTGCA AATCTGATACAAAGTGGCAAGTTCCAGCTTCATCTGGACTCTAAGACATTCTCAGCTGATACCATCTTGT ACTTTCTCAATGGGGACAGATTTGTCACCTCTCCCATGACTCAGCTTGGATGCTTGGAAGGATTTTACAG AGTTTCAACCACCAGTCAGGACCCTCTGGGCTGTGTGAAATGCCCTGAAGGAAGCTTCTCTCAGGATGGA AAATGCACTCCCTGTCCTGCTGGCACCTACCAAGGGCAGGCAGGCAGCTCAGCTTGCATCCCATGCCCAA GGGGCAGAACAACCACCATCACTGGAGCTTTCAGCAAGACCCATTGTGTCACTGACTGTCAGAGGGATGA GGCCGGCCTACAGTGTGACCAGAATGGTCAGTACCAAGCCAACCAGAAGGACATGGACAGTGGAGAAGTT TTCTGCGTGGATAGCGAGGGGCAGAGACTACAGTGGTTACAGACAGAGGCTGGACTCTCAGAATCCCAGT GTCTGATGATGCGGAAATTTGAGAAAGCTCCAGAATCCAAGGTTATCTTTGATGCCAGTTCTCCTGTGAT AGTCAAGTCCAGAGTCCCCAGTGCCAACTCCCCACTGGTGCAGTGCTTAGCAGATTGTGCAGATGATGAA GCCTGCAGCTTCGTCACAGTGTCCTCAATGTCATCAGAGGTTTCATGTGATCTTTATTCTTGGACAAGAG ACAACTTTGCCTGTGTGACTTCTGATCAGGAAGAAGATGCTGTGGACAGCTTGAAGGAAACCAGCTTTGG AAGCCTTAGGTGCCAGGTGAAAGTGAGGAATAGTGGCAAGGATTCTCTGGCTGTGTATGTGAAGAAAGGC CATGAATTTACTGCATCTGGTCAGAAGAGCTTCGAACCAACAGGTTTTCAAAATGTGCTCTCGGGGTTGT ATAGCTCTGTGGTGTTTTCAGCCTTGGGAACCAATCTGACAGATACACACCTCTTCTGTCTGCTTGCCTG TGACCAGGATTCCTGCTGCGATGGCTTCATCGTCACACAGGTCAAAGAAGGGCCTACCATCTGTGGACTT CTCAGTGCTCCTGACATTCTGGTTTGCCACATCAATGACTGGAGGGATGCCTCTGATACTCAGGCCAATG GTACATGTGCTGGGGTGACGTATGACCAAGGGAGCCGCCAGATGACCATGAGTCTGGGAGGCCAAGAATT CTTGCAGGGTCTGACGCTCTTGGAGGGAACTCAAGACAGCTTTATCAGCTTCCAGCAGGTCTACCTCTGG AAAGATTCTGACATAGGGTCTCGGCCTGAATCTATGGGATGTGGAAGGGGAATGGTGCCCAAGTCAGAAG CTCCAGAAGGAGCAGATATGGCAACAGAACTTTTCTCCCCTGTGGACATTACACAGGTCATTGTCAATAC AAGCCACTCATTGCCCAGCCAGCAGTACTGGCTCTCCACACACCTGTTTTCAGCAGAGCAAGCAAACCTG TGGTGCCTTTCTCGGTGTGCCCAGGAGCCTGTTTTCTGTCAACTTGCAGACATAATGGAAAGCTCATCTT TGTATTTCACATGCTCCCTATACCCAGAAGCTCAGGTCTGTGACAATGATGTGGAATCTAATGCCAAGAA CTGCAGTCAGATTCTGCCCCGCCAACCAACAGCCCTCTTCCAGAGGAAAGTTGTGCTGAATGACCGAGTG AAGAACTTTTATACTCGCCTGCCATTCCAAAAGCTCTCAGGGATTTCCATCAGAGACAGAATCCCCATGT CTGAAAAACTCATTTCCAATGGGTTCTTTGAGTGTGAACGCCTCTGTGACAGAGACCCATGCTGCACTGG CTTTGGTTTTCTAAATGTCTCCCAGATGCAAGGAGGAGAGATGACCTGCTTGACTCTGAACAGCATGGGG ATTCAAACATGCAGTGAGGAGAATGGAGCAACCTGGCGTATTTTGGACTGTGGCTCTGAAGACACTGAAG TCCACACCTATCCCTTTGGATGGTACCAAAAGCCTGCTGTGTGGAGTGATGCTCCCAGCTTCTGCCCATC TGCTGCCCTGCAATCACTCACTGAGGAGAAGGTTGCTTTGGACTCCTGGCAGACTCTGGCCCTATCTTCA GTGATTATAGATCCATCCATCAAGCACTTTGATGTTGCCCACATCAGCATTTCAGCCACCAGGAACTTCT CCCTGGCCCAAGACTTCTGCTTACAGGAATGTTCCCGTCATCAGGACTGCCTTGTCACTACCCTGCAGAT CCAGCAAGGGGTTGTGAGGTGTGTGTTCTACCCTGATATACAGAGCTGCGAACACAGTCTACGGAGCAAG ACCTGCTGGCTTCTGCTCCATGAAGAGGCTGCCTACATCTACCGGAAATCTGGAGCCCCTTTGCACCAAT CTGATGGAATATCCACACCTTCTGTACACATTGATTCCTTTGGCCAGCTTCAGGGTGGGTCCCAGGTCGT CAAGGTGGGCACTGCCTGGAAACAAGTATATCAATTCCTTGGAGTTCCATATGCTGCCCCACCCTTGGCA GAAAACCGTTTCCAGGCACCAGAGGTCTTGAATTGGACAGGCTCCTGGGATGCCACCAAGCTAAGGTCCA GCTGCTGGCAGCCAGGTACCCGTACCCCAACACCTCCTCAAATCAGTGAAGACTGCTTATATCTCAACGT GTTTGTCCCTGAGAACCTGGTCTCCAATGCATCAGTTCTGGTGTTCTTCCACAATACTGTGGAGATGGAA GGCAGTGGAGGTCAGCTGAACATTGATGGCTCCATTCTGGCTGCAGTTGGCAACCTCATCGTAGTCACTG CTAACTACAGACTGGGGGTCTTTGGCTTCTTGAGCTCAGGCTCTGATGAAGTAGCTGGCAACTGGGGGCT ACTGGACCAAGTGGCAGCTCTAACATGGGTGCAGACCCACATTGGAGCATTTGGTGGGGACCCTCAACGT GTGACACTGGCAGCAGACCGTGGTGGGGCTGATGTGGCCAGCATACACCTTCTCATCACCAGACCTACCA GACTCCAGCTCTTCAGGAAGGCTCTGCTTATGGGAGGCTCAGCATTGTCACCGGCTGCAATTATCAGCCC AGATAGGGCCCAACAGCAAGCTGCAGCCTTGGCAAAGGAGGTTGGATGCCCTAACTCATCCGTCCAGGAA GTGGTCTCCTGCTTCCGCCAGAAGCCTGCCAACATTCTCAATGAAGCTCAGACCAAGCTCCTGGCTGTGA GTGGCCCTTTCCACTACTGGGGTCCTGTGGTCGATGGCCAGTACCTACGTGAACTTCCTTCCAGACGGTT GAAGAGGCCTCTGCCAGTGAAGGTGGATCTGCTCATAGGAGGTTCTCAGGACGATGGGCTTATCAACAGG GCAAAGGCTGTGAAGCAATTTGAAGAAAGCCAAGGCCGAACCAACAGTAAAACAGCCTTTTACCAGGCAC TGCAGAATTCACTAGGCGGTGAGGACTCAGACGCCCGCATCCTTGCTGCTGCCATATGGTATTACTCCCT GGAGCACTCCACAGATGACTATGCCTCCTTCTCTCGGGCACTGGAGAATGCCACCCGGGACTACTTTATC ATCTGTCCCATTGTCAACATGGCCAGCCTCTGGGCAAGGAGAACCCGTGGAAATGTCTTCATGTACCATG TCCCCGAAAGCTATGGCCATGGCAGCCTAGAATTGCTGGCAGATGTTCAGTATGCTTTTGGACTGCCCTT CTACTCTGCCTACCAGGGCTACTTCTCAATGGAGGAGCAGAGCCTCTCTCTGAAAGTCATGCAGTATTTC TCCAACTTCATCAGATCAGGAAATCCTAACTACCCACATGAGTTCTCACAGAAAGCAGCTGAATTTGCCA CACCCTGGCCAGACTTTGTTCCTGGAGCTGGAGGAGAGAGCTACAAGGAGCTCAGTGCCCAGCTTCCCAA TCGGCAGGGCCTCAAAAAAGCTGACTGTTCCTTCTGGTCCAAGTACATCCAGACTCTGAAGGATGCAGAT GGAGCCAAGGATGGACAGTTAACCAAGAGTGGAGAGGAGGACTTGGAAGTGGGGCCTGGGTCAGAAGAAG ACTTCTCAGGCTCACTGGAGCCTGTCCCCAAGAGCTACAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030988
Insert Size	8307 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030988.2, NP_112250.2
RefSeq Size	8461 bp
RefSeq ORF	8307 bp
Locus ID	24826
Gene Summary	precursor to thyroid hormone; required for thyroid hormone generation [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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