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Gck (NM_012565) Rat Untagged Clone
CAT＃: RN213355
Gck (untagged ORF) - Rat glucokinase (Gck), (10 ug)
CNY 6,080.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLK; GLUKA; RNGK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213355 representing NM_012565
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGGATACTACAAGGTGTGGAGCCCAGTTGTTGACTCTGGTCGAGCAGATCCTGGCAGAGTTCC AGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGTGGCCTGAGGCT GGAGACCCACGAGGAGGCCAGTGTAAAGATGTTACCCACCTACGTGCGTTCCACCCCAGAAGGCTCAGAA GTCGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGAGTGATGCTGGTCAAAGTGGGAGAGG GGGAGGCAGGGCAGTGGAGCGTGAAGACAAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACGGG CACTGCCGAGATGCTCTTTGACTACATCTCTGAATGCATCTCTGACTTCCTTGACAAGCATCAGATGAAG CACAAGAAACTGCCCCTGGGCTTCACCTTCTCCTTCCCTGTGAGGCACGAAGACCTAGACAAGGGCATCC TCCTCAATTGGACCAAGGGCTTCAAGGCCTCTGGAGCAGAAGGGAACAACATCGTAGGACTTCTCCGAGA TGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGGCAATGGTGAACGACACAGTGGCCACAATG ATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGGGCACTGGCTGCAATGCCTGCT ACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGATGAGGGACGCATGTGCGTCAACACGGAGTG GGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTACTGGAGTATGACCGGATGGTGGATGAAAGC TCAGCGAACCCCGGTCAGCAGCTGTACGAGAAGATCATCGGTGGGAAGTATATGGGCGAGCTGGTACGAC TTGTGCTGCTTAAGCTGGTGGACGAGAACCTTCTGTTCCACGGAGAGGCCTCGGAGCAGCTGCGCACGCG TGGTGCTTTTGAGACCCGTTTCGTGTCACAAGTGGAGAGCGACTCCGGGGACCGAAAGCAGATCCACAAC ATCCTAAGCACTCTGGGGCTTCGACCCTCTGTCACCGACTGCGACATTGTGCGCCGTGCCTGTGAAAGCG TGTCCACTCGCGCCGCCCATATGTGCTCCGCAGGACTAGCTGGGGTCATAAATCGCATGCGCGAAAGCCG CAGTGAGGACGTGATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCGAGCTTCAAG GAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTGCGAAATCACCTTCATCGAATCAGAGGAGG GCAGCGGCAGGGGAGCCGCGCTGGTCTCTGCGGTGGCCTGCAAGAAGGCTTGCATGCTGGCCCAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_012565 |
| Insert Size | 1398 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012565.2, NP_036697.1 |
| RefSeq Size | 2343 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 24385 |
| UniProt ID | P17712 |
| Gene Summary | catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate coding exon and uses an alternate splice junction at the 3' end of another coding exon compared to variant 1. The resulting isoform (2) has the same N-and C-termini but differs in an internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213355 | Gck (Myc-DDK-tagged ORF) - Rat glucokinase (Gck), (10 ug) |
CNY 6,080.00 |
|
| RR213355L3 | Lenti ORF clone of Gck (Myc-DDK-tagged ORF) - Rat glucokinase (Gck), (10 ug) |
CNY 7,890.00 |
|
| RR213355L4 | Lenti ORF clone of Gck (mGFP-tagged ORF) - Rat glucokinase (Gck), (10 ug) |
CNY 8,650.00 |
