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Cdc73 (NM_001024769) Rat Untagged Clone
CAT＃: RN213220
Cdc73 (untagged ORF) - Rat cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Cdc73), (10 ug)
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Hrpt2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213220 representing NM_001024769
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGTGCTCAGCGTCCTGCGACAGTACAACATCCAGAAGAAGGAGATTGTGGTCAAGGGGGATG AAGTGATCTTCGGGGAGTTCTCCTGGCCCAAGAATGTGAAGACCAACTATGTAGTTTGGGGGACCGGAAA GGAAGGCCAACCCAGAGAATACTACACATTGGATTCTATCCTATTTCTCCTTAATAATGTGCACCTTTCG CATCCTGTTTATGTCCGACGTGCAGCTACTGAAAATATTCCTGTGGTTAGAAGACCTGACAGAAAGGATC TCCTTGGATATCTCAATGGGGAAGCATCAACATCAGCAAGCATAGACAGGAGTGCCCCCCTGGAAATAGG TCTTCAGCGGTCTACTCAAGTCAAAAGAGCTGCAGATGAAGTTTTAGCAGAAGCAAAGAAACCACGAATT GAGGATGAAGAGTGTGTGCGCCTTGATAAAGAGAGATTGGCAGCCCGTTTGGAAGGCCATAAAGAAGGAA TCGTGCAGACTGAACAGATTAGGTCTTTATCTGAAGCCATGTTAGTGGAAAAAATTGCTGCAATCAAAGC CAAAATTATGGCTAAGAAAAGATCTACTATCAAGACCGATCTAGATGATGATATAACTGCCCTTAAGCAG AGGAGTTTTGTGGATGCTGAGGTAGATGTGACCAGAGATATTGTCAGCAGAGAGAGAGTATGGCGGACAA GAACAACTATCTTACAGAGCACAGGAAAGAATTTTTCAAAGAATATTTTCGCAATTCTTCAATCAGTAAA AGCCAGAGAAGAAGGGCGTGCCCCCGAACAACGGCCAGCTCCAAATGCAGCCCCTGTGGATCCTACATTG CGTACAAAACAGCCTATTCCAGCTGCCTACAACAGATATGACCAAGAAAGATTCAAAGGCAAAGAAGAAA CGGAAGGTTTTAAGATCGATACTATGGGTACCTACCATGGCATGACGCTGAAATCTGTCACGGAAGGTGC ATCTGCCCGTAAGACTCAAACTCCTGCAGCCCAGCCTGTACCTAGACCAGTGTCTCAAGCAAGACCTCCC CCAAATCAGAAGAAAGGGTCTCGAACACCCATCATTATTATTCCTGCAGCTACCACTTCTTTAATAACCA TGCTTAATGCAAAAGACCTTCTACAGGATCTGAAATTTGTCCCATCAGATGAAAAGAAGAAACAGGGATG TCAACGAGAAAATGAAACACTAATACAGAGAAGAAAAGACCAGATGCAACCTGGGGGCACAGCAATTAGT GTCACAGTCCCTTACAGAGTAGTAGACCAGCCTCTGAAGCTTATGCCTCAAGACTGGGATCGAGTCGTAG CTGTTTTTGTACAAGGTCCTGCTTGGCAGTTCAAAGGTTGGCCATGGCTTCTACCTGATGGATCACCAGT TGACATATTTGCAAAAATTAAAGCCTTCCATCTCAAGTATGATGAAGTTCGTCTAGATCCAAATGTTCAA AAATGGGATGTAACAGTATTAGAACTCAGCTATCATAAACGTCATTTGGATAGACCAGTTTTCTTACGTT TCTGGGAAACATTGGATAGATACATGGTAAAGCACAAATCCCACTTGAGATTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024769 |
| Insert Size | 1596 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024769.1, NP_001019940.1 |
| RefSeq Size | 2101 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 304832 |
| UniProt ID | Q4V8C8 |
| Gene Summary | Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression. Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the cleavage and polyadenylation specificity factor (CPSF) complex and the cleavage stimulation factor (CSTF) complex, and with Wnt signaling. Involved in polyadenylation of mRNA precursors (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213220 | Cdc73 (Myc-DDK-tagged ORF) - Rat cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Cdc73), (10 ug) |
CNY 6,840.00 |
|
| RR213220L3 | Lenti ORF clone of Cdc73 (Myc-DDK-tagged ORF) - Rat cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Cdc73), (10 ug) |
CNY 8,740.00 |
|
| RR213220L4 | Lenti ORF clone of Cdc73 (mGFP-tagged ORF) - Rat cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Cdc73), (10 ug) |
CNY 9,600.00 |
