F5 (NM_001047878) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ac2-120
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212654 representing NM_001047878 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAGCTCAGAAGGTCAAGTCCAAGAGAAGCAGGCGGAGATCCGGAGGAGTGACAAAAATCATGTCG GGGCCTTTCTATATGTTCCTCTATATGTCTTTCATGATCTTGAATATAAACAATTTCAAACCTATCCTTC CTTAGGACTTCTCGGACCAACTTTATATGCTGAAGTTGGCGACACCATCAAGGTTCACTTTAGAAACAAA GCAGACAAACCTCTAAGCATCCACCCTCAAGGAATTAGATACAGCAAATTTTCAGAAGCTCAGCTGTTTG CGGAGGAGGAAATGGACTTTGCAGCACCATTCATCCTCCTTCGATGTTTATTTGACCCTCCCTCACTCAG TCAGCAAGCATTGGTTCTTCTTCCAAAACGGAAGACTGGTTTCTACGTCACCCCTCTACTCCTGGAGGGA GTGCCCGGTGTTCATAGGTTGTCAATGGGTCTTTACACAATGAAGCGAGGCATCTTCACCCTGGGACAGA CTGGCTTCATGTGCCCAAGGAGTAGGCTGCATGCAGGAGCTGGATGTGGAGACCGCAGGGGTGGGGAAGA GCCAGAAAGAGCTAGCGCATCACCCTTTCTCCACACTGGCGAGTGGTGGAAAACTCTGGATGTCTTTGTG GCGGTCACTTCTCCAGGGGCTTCTTACCTGGACCACACATCCCCGGCTGAGAGGAAGGACGATGCTGTGG CTGCTGGAGAGGAATACACCTATGAATGGTTCATCGGTGAGGACAGCGGGCCCACACCTGATGACCCACC ATGCCTCACCCACATCTACTACTCCTATGAAAACCTGACCCAGGATTTCAACTCAGGCCTGATTGGGCCT CTGCTCATCTGCAAGGAAGGCACCCTGACTGAGGATGGGACTCAGAAGATGTTTGACAAGCAACACGTGC TTCTGTTTGCTGTATTTGATGAAAGCAAGAGCTGGAGCCAGTCACCAGCCCTCATGTACACAGTCAACGG CTTTGTGAATAGGACAATGCCAGATGTAACAGTCTGTGCCTACGATCATGTCAGCTGGCATCTGATTGGG ATGAGCTCGGGACCAGAATTGTTTTCTATTCACTTCAACGGCCAAGTCCTAGAGCAGAACCAACATAAAG TGTCCACCGTGACTCTGCTGCAGACTGTAGGCATCTTTATCCGACCAATAGCTTTAAATACTGTGCAAGG TTACATAGCATCCCGTGGTGAGGATCTCCTTGTCCCTTGGGCAACCAGAGCTGCTGGGATGCAGGCTTAT ATTGACATTAAAAACTGCCCAAAGAAAACCAGGAGCTCCAAGACGCTCACCCGAGAGCAGCGGCGGCACA TGAAGAGGTGGGAGTACTTCATCGCCGCAGAGGAAGTCATTTGGAACTACGCACCTGTGATACCCGCAAA CATGGACAAGCATTCTTATCCACTAAGTCCCATGGGAATGTGGCCTGATGATAGTAACAGGAACAATGGC AGTAGAATGCTAGCTACTGTTAACCCTTGTAGTAGGTGTTTGCTAAGGCTGTCACATCAGTTCTACAAGC TAGGTGCTATTCTTGTGTTCTTGTATCTAAGGGAAGGTGCCAGAAAATCGGAAGCTCTAGTAGTTCGCCA TATGCCATTTCTAAGGGTAGCAGACATGGAGCAGGAGGTCGTGTTTGCTATGTTTGATGAGAACAAGAGC TGGTACATCGAGGACAACATCAACAAGTTTTGTGAGAATCCCGATGAGGTGAAGCGTGATGACCCCAAGT TTTATGAATCAAACATCATGAACACTATCAATGGGTACGTGCCTGAGAGCATATCCACCCTAGGATTCTG TTTTGATGATGCCGTACAGTGGCACTTCTGCAGTGTGGGAACTCATGATGACATTCTGACCGTCCACTTC ACTGGGCACTCATTCATCTATGGGAGGAGGCACGAGGACACCTTGACCCTGTTCCCCATGAGTGGTGAAT CTGTGACTGTCGTGATGGATAATATTGGAACTTGGATGTTGACCACCATGAGTTCCAATCCAAGACGCAG AAACCTAAGATTGAGATTCAGGGATGTTAAGTGTAACCGGAATGATGATGACGATGAAGACTCATATGAG ATTTATCAACCTCTTGAACCTACATCCATGACAACTCGGAAAATTCATGATTCTGTGGAAAATGACTTTG GCATAGAAAACGAAGATGATGATTACCAGTACGAACTGGCGTCAACACTAGGAATTAGGTCATTCAGAAA GTCATTGTTGAATCCGAAGGAAGACGAGTTCAATCTCACTGCTCTCGCTCTAGAAAACAGCTCTGAATTC ATATCTCTAAGCACAGACGGAGTTGTTGACTCAAAATCCTCAAGAAACCTTAGTAAGATCACCAATAATA ACCTCAACGACTCTCAAAGAACACTTTCTGGCTCAGGAGCCACCATAGCTGGTATCCTACTTGGAAACCT TACTGGCTTAGGTAAGAACTCCGTCCTCAACCCTTCAACAGAATATCATTCCAGCTCATATTATGAAAAT GATATGGAAGACCCACAGTCGAACATCACAGTGGTATACCTACTTCCTCTTGGTGCAAAAGGATCAGGGA GTCGAGAACAGACTAAACCTAAAACAATCAAGACAGGAAGACCCCACAGGATGAAGCACAGGTTCTCCTG GATGAAAGCTCCAGCCGGAAAAACTGGGAGGCATTCAAATCCAAAGAATACTTCTTCCAGAATGAAGTCT GAGGAGGACATTCCTAGTGATTTGTTACTCTTAAAACAAAAGGTCGCATCCAAACTTTTGAACAGACAGT GGCATATGGCTTCTGAAAAGGGTAGTTATGAAATAATACCAGCAAATGGTGAAAACACAGATATTGATAA GCTGACAAACAGTCCTCAAAATCAGAATATCTCAACGCCTTGGGGAGCTAGCACCTCTCGCATAAACACG ACAGGGAAGCCAAGTAACCTCCCAACATTTTCTAGATTTAGGCATAAATCTCCACATGTAAGACAGGAGG AGGAAAGCGGTGACTTTAAGAAAAGACAGTTGTTCATCAGGACACGGAAGAAGAAGAAGAACAGGAAAAG ACTACTCAATCATTCATTACTACTCCACAAATCCAATGAAACAGCTCTTTCCACAGATCTGAATCAGACC TCCCCTCCAGTGAGTACTGACAGGTCACTTCCTGACTATAATCAGAACCCTTCAAATGATACTGAGCAGA TGAGCTCTTCTTTAGACCTTTTTCAGTCAGTGCCACCAGAGGAACACTCTCCAACATTTCCTACCCAAGA TCCTAATCAAACACACTCTACCACAGATCCTAGCTACAGATCCTCTCCTCCAGAGCCCAGTCAAGGGATC GATTATGACCTAAGCCATGAATTTTATTCTGATGACATTAGTCAAACATCTTTCTTTCCAGACCAAAGTC AAAAGTCACCTCTCACTTCAGATGATGGCCAAGCAATCCCTTCCTCAGACTTAAATCTCTTTACCATCTC TCCAGAATTGGATCAAACAATTATTTACCCAGACCTGGATCAGTTGTTCCTTTCTCCAGATGACATCCAG AAGACCTCCTCCCAAGACCTGGGTCAGGTGACCCTTTCTCCAGATGAAAACCAAGAGACCTCCTCCCAAG ACCTGGGTCAGGTGACCCTTTCTCCAAATGAAAACCAAGAGACCTCCTCCCAAGACCTGGGTCAGGTGAC CCTTTCTCCAGATGACATCCAGGAGACCTCCTCCCAAGACCTGGGTCAGGTGACCCTTTCTCCAGATGAA AACCAAGAGACCTCCTCCCCAGACCTGGGTCAGGTGCCCCTTACCCCAGATGACAACCAGAAGACTTCCC CAGACCTAGGTCAGGTGCTCCTTTCTCTAGATGACAAGCAGAAGACTTACTTCCTAGACCCAGGTCAGGT ACCCATCTCCTCAGACCAAAGCTGGGAGACCTCCTCCACTGACCTTAGCCTACTGACTCTCTCTCCGAAA TTTGGTCAGACAGTCATTTCCCCAGACTTGGATAAGATGCCCCTCTCTTCAGACAACAGTCAAGTTACCC TTTCCCCAGACCTCAGCCTCTTGACCCTCTCACCAGATTTTAATGAGATAATACTATCCCCAGACATTGA TCAGGTGACCCTCTCTCCAGACCTCATCCAGACAAGCCCTGCTCTTAATCACAGGCACAAAACATCCTCT GCAGACCCTGGTCAAGCATCCTACCCCCCAGATTCTGGTCAGTCTTCACCTCTTCCAGAACTGAATCAGA CTCTTCCTCATCCAGACCTCATTCACATGCAGCCTCCTTTACTATCTCCCACACCTAATGACACTTCTTT GTCAAAGACATTTAACCCCCTTGTTGTAGTAGGTCTCAGTAGAGTTGATGGAGACGATGTTGAGATGATT CCAAGTGAGGAGCTAGAGAGCATTGACGAAGATTATCCTGAGGATGACTATGTAACCTACAATGACCCCT ACAAAACAGACACCAGGGCAAATGTCAACTCCTCCAGAAACCCTGACACTATTGCAGCATGGTATCTCCG TAGCTTCGGTGGGAACAAAAAATTCTACTATATTGCTGCTGAAGAAATATCCTGGGATTATTCAAAATTT GCACCAAGTGAAATGGACAATGAAGAAACAGACAACACTCCAAAGGACACCACATACAAGAAAGTTGTTT TCAGAAAATACCTTGATAGTACTTTCACAAGCCGTGATCCTCAGGGGGAATATGAGGAGCACCTTGGCAT TCTTGGCCCTGTAATACGTGCTGAAGTGGATGATGTGATCCAAGTTCGATTTAAAAATTTAGCATCCAGA CCATATTCTCTTCATGCCCATGGACTTTCCTATGAAAAGTCCTCAGAGGGAAAGAATTACGAAGATGACT CTCCTAAATGGTTTCAGGAAGATGATGCTGTCCAGCCCAATAGCAGTTACACATATGTATGGCATGCCAC TGAGCGCGCGGGACCAGAGAACCCCGGTTCTGCCTGCCGGGCTTGGGTCTACTATTCTGCAGTGGATGTG GAGCGAGACATCCACTCAGGCTTGATCGGCCCCCTTCTGATCTGCCGGAAAGGAACACTTGACAGGGCGA GCAACCTGCCTCTGGACATGAGAGAGTTTGTCTTGCTCTTCATGGTCTTTGATGAGAAGAAGAGCTGGTA CTATGAAAAGTCCAAAGGGTCATGGAGAATTGAATCTCCAGAAGCGAAAAATTCGCACGAGTTTTACGCA ATTAATGGGATGATTTACAACCTGCCCGGCCTGAGAATGTATGAGCAAGAGTGGGTGAGGCTGTACCTGC TGAACATGGGCGGCCCCCAAGATATTCACGTGGTTCACTTCCATGGCCAGATCCTGCTGGATAATAGGAC CAAACAGCACCACTTAGGGGTCTGGCCCCTCTTGCCTGAGTGCAAGATGCCAATGGGTCTAAGTACTGGT GCCATATCTGACTCACAGATCAAGGCTTCAGAATATCTGAGCTGCCTTGGAGGACATGCTAGAGGACTGA CAGACCCTGTCTGGTACTCCATGAGCCCAGACAAGATTCCTAGATCATTCCTCATTCCCAGGCTCCTCCC CAAAGAAAAATATTCAAATGCAGAGAGCCTGTCTGTCATCATTGTGCAAACCACCTTGAGTCTGTTTCTC TTGGTACTTTATTCTTTTGCTGGCAATTCAGATGCCTCTACGATAAAAGAGAATCGATTTGACCCACCTA TTGTGGCTAGATACATTAGGATACATCCCACGAAATCCTATAACAGACCCACCCTTCGGTTGGAGCTGTT GGGCTGTGAGGTGAACGGATGCTCCACACCACTGGGCCTGGAAGATGGAAGGATACAAAACAAGCAAATT ACTGCATCTTCATTTAAAAAGTCATGGTGGGGAAGCTACTGGGAGCCTTCCCTTGCCCGCCTGAATGCCC AGGGCCGAGTGAATGCCTGGCAAGCCAAGGCAAACAACAACAAGCAGTGGTTACAAATTGATCTGCTCAA AATCAAGAAGGTAACGGCCATCGTAACTCAGGGTTGCAAGTCTCTGTCCTCTGAGATGTATGTGAAGAGC TACAGCATCCTGTACAGTGACCAGGGTGTCTCCTGGAAACCCTACCGGCAGAAATCCTCCATGGTGGACA AGATTTTTGAAGGGAATAGCAATACCAAAGGGCATATGAAGAACTTTTTCAACCCACCTATTATTTCCAG ATTTATCCGAATCATTCCTAAAACATGGAACCAAAGTATTGCACTTCGCCTGGAACTCTTCGGCTGTGAC ATTTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001047878
Insert Size	6309 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001047878.1, NP_001041343.1
RefSeq Size	6315 bp
RefSeq ORF	6309 bp
Locus ID	304929
Gene Summary	may play a role in blood coagulation [RGD, Feb 2006]

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