Phf3 (NM_001108791) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212491 representing NM_001108791 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATAGTTGATACATTTAATCATTTAATTCCTACTGAACACTTAGATGATGCCCTATTTCTAGGAT CCAACCTGGAGAATGAAGTCTGTGAGGATTTTAGTACAAGTCAAAATGTCTTAGAGGACTCGCTGAAGAA CATGCTCAGCGATAAGGATCCTATGCTAGGATCTGCAAGTAACCAGTTCTGTTTGCCTGTTTTGGATAGC AATGATCCCAATTTCCAGATGCCTTGTACAACAGTTGTTGGTCTTGACGATATTATGGATGAAGGAGTTG TTAAAGAAAGTGGTAATGATACCATTGATGAAGAAGAATTGATTTTACCTAACAGGAGTTTGAGGGACAG AGTAGAAGACAATTCAGTAAGATCACCAAGAAAATCACCTCGTTTAATGGCACAAGAACAAGTAAGAAGT TTGCGACAAAGCACTATTGCCAAGCGTTCAAATGCAGCACCTCTAAGCACAAAAAAGCCATCTGGGAAGA CTTTCTCTACCTCTAAAGCAGGGGTGAAACCACCAGAAAGATGTCAGGGTAAAGAAGAAGTTTATGCATC ACTGAAATCTGAGCACCCTAAGGAGAGCAGAAGGAATAGCCGACATGCTGAACACATGGACATGGTGCTG GAAGTCTCAGCATCTTCAGCTGATTCTCCAGTGCCATCTTGTGTCGGAATGAAGGAAGCAGCTGAGCTCC ATCCCAGGCATGCGTGTAATAACCAGGGCGAAGTGAGTGCGCCATCCCCGGACTTAAGTTGTTCGCCTCC CTCAGAGGCTAGTGCTAGTGCTGGTGTGGGAGGAAAGAGAACCGAAGCTCTGATGGAGTGTAAGACTAGC AGTAGCCCATTATTTAAGGTTTCAGTTAAAGAGGATGAGCAAAGTGACCTTGTTTCCGGTGAACTGGATG ACACCATTGAAGGAGTGAATGCAGTGGGAAGACTTGAGCAAGAGTCAGAGGAGATAAAGTTCTCCTGCGA AGGTGACCGGATGAGCACGGAACCTGAATCTTCTGATGTATCCAGTGACTCTGCTTGTACCGATAAAAAT AAGACAGAAAAGAATGAAGGGGCGCAGTGTCATTTGGAATTGAAGAATACTGTGGATATTGTTGATAAAC CTGAGAACTCTCCTCCAAGCAATCAAATGGAAATGCTGGACTATGGTGAGGACGTGGGGACTAATGATGC TCAGTTACAGAGCACAGAGTTTACTAAGTCACATTTGGAGGAGGTTGACACTTGTGCTTTTGAACCAGAA ACTAGTACTTTTGAAAATACTATTTGTGATGTTCCTGACCAAAATTCAAAGCAATTAAATATTACTCAAA GTATTAAAATGGAAACATCAAATCTTCAGGATGATAGAAGTGGTTTAGGGCCCAAAAACATGAAATCCAA ACACACAAAATCTGTAACTCATTCTAAACCAAGCATGACCATAGAGACACCAAGAAAAACTGTGGCAGTA AAGCCTGAAGTAGGTCACAGCAAAACTAAATCTAATGCCAAGACTATGAAGCGAAATGCTGATGAACCAG AGCCTCAGCCTGATTGTCAGAGGCCAGTCAAAGTGAGAAAGAAACAAGTTGATAAGGACTTGAAGAGTCA GAGTTGTAATTCTGGAGTTAAATCTGTGAAAGGCCAAGTCCATTCTGTATTGAAAAAAATACCCCAGGAT CAAACTGCAATGCAAATTTCCAAACCTTTAACTCATTCTGTTAATGATAAACTTCCTGGTCATTCTGGTC ACTCCTTAGAGAAGCCTCCTCATCCTGGACACACTGGACACTTAGTGCATTCCAATCAGAAGCAGAGTCA TAAGCCTCAGCCACAGGCGGGAGCGGTGAGGGTCACTAGCCATGTGAGGGATGAGCATGAGCATCCAGGC ACTGAGCACCCTAAGGAGGAGGACAAACTGAAGCCAAAAAAACTCGATAGGAATCTACAGCCTCGCCAAA GGAGAAGCAGCAGAAGTTTTTCTCTGGATGAGCCTCCACTGTTTATCCCAGACAACATAGCTACTGTAAA AAAAGAAGGGTCAGATCAGACCACCTCTGTTGAAAACAAATATATGTGGACTCCCAGCAAGCAATGTGGG TTTTGCAAAAAACCACATGGCAACAGGTTTATGGTTGGCTGTGGGAGATGTGATGACTGGTTCCATGGGG ACTGTGTTGGACTAAGTCTTTCTCAAGCGCAGCAAATGGGAGAGGAAGACAAAGAGTATGTTTGCATAAG ATGCTGTGCTGAAGAAGATAAAAAGACGGACATACTAGATACAGAGACTTTGGAAACCCAGGCTTCCCTC GAAGCCCACAGTGAAGATAAAAGGGTGGAGTGTGGAAAGCTGACATCGTCGTCATCGAAGCACTCGGTCA CCGAGGATAAACACAGACACATGGACGACCCTGGCAGGCACAAGGTCAAAATTGTAAAACGGGACTCTGG TGAGGGAAAAACTTCCTCTGATAGTAGAGATAATGAAATTAAAAAATGGCAGCTAGCCCCTCTTCGCAAG TTGGGCCAACCACTTTTACCTCGAAGGTCATCAGAAGAAAAAAGCGAAAAAATAGCAAAAGACTCTGCAG CTCTCACCTGCGCAGGAGAGAAAGCAGCGAGATCAGGTACTCATGAGAAGCAAGAGACAAAGAAGAAGAA AGTTGAAAAGGGTGGACCTAATGTGCACCCTCCTGCCGCTGCTGCCATCAAGCCTTCTGCAGATCAGATC AGACAGAGTGTCCGGCACTCCCTTAAGGATATTCTTATGAAAAGACTTACGGACTCAAACTTGAAGATCC CAGAGGAAAAGTCAGCAAAGGTTGCCACAAAAATTGAAAAGGAACTTTTCTCTTTTTTTCGGGACACAGA TGCCAAGTATAAGAATAAATACAGAAGTTTGATGTTCAATCTAAAAGATCCTAAGAACAATATATTATTT AAGAAAGTTCTAAAAGGAGAAGTAACCCCTGATCATCTTATAAGAATGAGTCCAGAAGAACTAGCGTCCA AAGAGTTAGCCGCGTGGAGACGGAGGGAAAACAGACACACCATAGAGATGATCGAGAAGGAACAGAGAGA AGTGGAAAGAAGACCAATCACGAAAATAACTCACAAAGGTGAGATAGAAATCGAGAGTGACGCCCCGATG AAAGAGCAGGAAGCAGCCATGGAGATCCAGGAACCTTCTTCAGCCAATAAGTCCTCGGAGAAGCCAGAAG CATCCGAGAAACAAAAAGAAGAGCTTGACTCCACATCCAAAGACACCACCAGTCAACATAGACAGCATCT TTTTGACCTGAACTGCAAAATATGCATAGGTCGAATGGCACCACCTGTAGATGATCTTTCTCCCAAAACC GTGAAGGTTGTTGTGGGCGGCGCCCGTAAACACTCCGACAACGAGGCGGAAAGTATAGCGGATGCGTTGT CTTCAACGACAAATATTCTGGCCTCTGAATTCTTTGAGGAGGAGAAACAAGAATCTCCCAAATCAACATT CTCTCCCACTCCACGTCCAGAGATGCCTGGAACTGTGGAGGTTGAGTCAACCTTCCTGGCTCGATTGAAC TTCATCTGGAAAGGTTTTATCAATATGCCATCTGTGGCGAAGTTCGTTACCAAAGCCTACCCAGTGTCTG GATCCCCCGAGTACTTGACAGAGGACCTACCAGATAGCATTCAAGTAGGTGGCAGGATATCGCCTCAGAC TGTTTGGGATTATGTAGAAAAAATAAAAGCATCAGGCACCAAGGAAATCTGTGTGGTTCGTTTCACACCA GTAACTGAAGAAGATCAGATTTCTTACACTTTGCTGTTTGCGTACTTCAGTAGCAGAAAGCGCTATGGTG TAGCCGCTAACAACATGAAGCAGGTTAAAGATATGTACCTTATTCCATTGGGCGCTGCAGATAAAATTCC ACACCCTCTCGTGCCTTTTGATGGACCTGGGCTTGAGCTGCACAGACCTAATCTGTTGTTGGGTCTGATT ATTCGTCAGAAGCTGAAGCGGCAGCACAGCGCTAGTGCGGGCCCCAGTCACACAGCCGAGACTCCCGAGA GTGCCCCAATATCCTTGCCACCTGACAAAAAAGGTAAAATGGAGTCATCCACAGAGGAAGCAGCAGAGGA AGAGAGTGACTTTTTCAATTCCTTTACAACTGTGTTACACAAGCAGAGAAACAAGCCCCCTCAGCCTCCT CAGGAGGACTTGCCAACTGCAGCTGAACCTCTCATGGAAGTCACCAAGCAGGAGCCACCCAAACCCTTAA GGTTCCTTCCTGGGGTCCTAATTGGCTGGGAAAATCAACCTTCTACTCTGGAGTTGGCAAATAAACCTCT TCCTGTGGATGACATACTCCAAAGCCTGTTGGGCACCACTGGCCAGGTTTATGAGCAGGCTCAGCCAGTT GTAGAGCAAAGCACTCTTAAAGAAATTCCTTTTATAAATGATCAAACTACCCCAAAAGTAGAGAAAATAG ATAAAGTGGAAGTGACTGAAGGTGAAGCCAAGGAGATAAAAGTTAAAGCAGAAAATGTTTCAGAGTCTAC AGGTAAAAACTCAGGAGTAGAAGAAACTTCACTGGTAGGCTCCTCTTCCATTTCTCCAGGGCCTTTGGCA AGTCTTAGTCTGAGAGGGAAACCACCAGATGTCTCTACAGAAGCATTCTTAACAAATTTATCAATTCCTT CAAAACAAGAGGAAACTGTGGAAAACAAAGAGAGAACATTAAAAAGACCACTGCTCCAAGATCAGGAGAA CAGTTCGCAAGATAATAGGACTTGTAGTAACTCTCCTTGCCGGCCTAGCACCGGAAAGGGAAGCGTAGAT GGAAATGTGAGCTGCAGTGAGAATCTTGTTGCTAACACAAGATCCCCACAGTTTATCAACCTGAAAAGGG ATCCTCGGCAAGCAGCAGGACGGAGCCAGCAGACAGTTTCAGACAGCAAGGATGGAGAGAGCTGCAGAAA CGGAGAAAGGCATTCTGTGTGTGCTCCATCATACAACAGAGAGCCCGTAGAACCCGTCAGTGGAGAGGGA AAGCCCTCTTCTCTGGAGAAGAGCTCGTGTGTAGAGCAGAGTGATGATTCAGAGGCTGTGCCAAACTCAT CCTCGGTAGAAAACTCAAATTCTTCACAAGCAGAACAAGCCAATCCTTTACAAGAGGATATTTTAACACA AAATATTGAAACCATCCACCCATTTAGAAGAGGATCAGCTACATCATCATCTCATTTTGAAGGTGGAAAC ACATGCCAGTCAGAATTTCCTTCTAAAAGCATCAACTTTACTCCCAGGAGTACCAGCCCCCGAGCGAGTA CAAACTTTTCACCAATGAGGCCACAGCAGCCCAACCTCCAGCACCTCAAGTCTAGTCCTCCTGGATTCCC ATTTCCAGGACCCCAGAATTTCCCTCCCCAAAGTGTGTTTGGATTCCCACCACATTTGCCACCTCCGTTA CTTCCGCCTCCAGGCTTTGGCTTTCCTCAGAATCCCCCCATGGCTCCTTGGCCACCTGTTCACGTCCCGG GCCAACCACAGCGCATGATGGGGCCCCTTTCACAAGCATCAAGGTACATGGGACCACAAAATTTTTATCA GGTAAAAGATATTCGGAGACCAGAAAGGCGACATAGTGACCCATGGGGTAGGCAAGACCAACAGCAACCA GATAGGCCATTTAATAGGGGTAAAGGGGACCGTCAGAGATTTTACAGCGATTCGCACCACTTGAAAAGAG ACCGTCATGACAAGGAATGGGAGCAAGAATCTGAAAGGCACAGGCACAGAGACAGAAGCCAAGAAAAGGA CAGAGACAGGAAAAGCAAAGAGGAAGCACATAAAGATAAGGAGAGACCACGGTTATCACACAGCGATCGA GCAGCAGATGGCAAAGCCAACAGAGAGAGTAAGAGTGCAGACAAGAAACCGGACAGGCCTAAAGGTGAAG ACCATGAAAAGGAGAAAGAGAGAGAGAAGAGCAAGCACAGAGAGGGGGAGAAGGACAGAGAGCGGTACCA CAAAGACAGGGACCACACTGACAGGGCGAAGAGCAAAAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108791
Insert Size	6063 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108791.1, NP_001102261.1
RefSeq Size	7863 bp
RefSeq ORF	6063 bp
Locus ID	363210

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