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Rrp8 (NM_001008346) Rat Untagged Clone
CAT＃: RN212043
Rrp8 (untagged ORF) - Rat similar to RIKEN cDNA 1500003O22 (RGD1308302), (10 ug)
CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1308302 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN212043 representing NM_001008346
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGAGGAGCCCGAATGGGTCGAGGCGGCCCCAGCCATCGTTGGCCTCCGGCCCGTGACAACACAGG TTCAGGCGGCGACTGCCCCGCCAGTCAAGGGCCGTAAGCGCCGCCATCTCTTGGCTACATTACGAGCCCT GGAAGCAGCATCTCTCTCCCAGCAATGCCCCAGCCTACCTGGCAGTGACTCTGAGGAAGAGGAGGAGGTA GGAAGGAAGAAGAGACACTTCCAAAGGTCCTCACTTGCCAATGTCTCAAAGGAAGTAGGGAAGAAAAGAA AAGGGAAGTGTCAAAAACAGGCACCATTTATCAGTGACTCTGAGGGGAAAGAAGTGGAAAGAACATGCCA CAGACAAGCTCCTCCTCTTGGCGGGATCTCTGCTGGAGAAGAAAAAGGAAAGAGGAAATGCCAGGAATAT TCTTATTTACACCCAACCCAGTCCCTGAACAGTGTTGACCAAACAGTTCACAATTCTAGGACAAGTACTG CTACACTTGACCCACCCAAGTCAAGCCGTGAGTCTGCATCACCTAACTCCTCACACACCCTGAGCCGCAA GCAGTGGCGGAACCGGCAGAAAAATAAGCGGAGACACAAGAACAAATTTCGGCCACTTGAGACACAGGAC CAGGTGCCTCTCAAGGCTTCCATAGAGGAGACTGAGGTGCCTCCTGCTCCAAAGTCAGACAGTCAAGAGA CTAGAGCTGGAGCCCTGCGAGCACGCATGACACAACGCCTGGATGGGGCCCGATTTCGCTACCTTAATGA ACAGCTGTACTCAGGGCCCAGCAGTGCTGCCCAATGCCTATTCCAGGAAGACCCTGAGGCTTTTCTCCTT TATCACCGTGGCTTTCAGAGACAAGTAAAGAAGTGGCCACTGCACCCCGTGGACCGCATTGCCAAAGATC TCCGTCAGAAGCCTGCATCCTTAGTGGTAGCTGACTTTGGCTGTGGAGATTGCCGCCTAGCTTCAAGTGT TCGGAACCCCGTGCACTGTTTTGATTTGGCTGCTCTGGACCCCAGGGTCACTGTGTGTGACATGGCCCAG GTACCTCTGGAGGATGAGTCTGTGGATGTGGCTGTGTTTTGCCTTTCACTAATGGGAACCAACATCAGGG ACTTCCTTGAGGAGGCGAATCGAGTGCTAAAGCCAGGGGGTCTTCTCAAAGTAGCGGAAGTCAGCAGCCG CTTTGAGGATATTCGGACCTTTTTGGGGGCTGTGACCAAACTTGGCTTTAAGGTCATCTACAAGGACCTG ACCAACAGCCACTTCTTCTTGTTTGACTTTGAAAAGACTGGACCTCCTCGAGTAGGACCCAAAGCCCAAC TCTCAGGCCTTAAACTTCAGCCCTGTCTCTACAAGCACAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001008346 |
| Insert Size | 1374 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008346.1, NP_001008347.1 |
| RefSeq Size | 2095 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 308911 |
| UniProt ID | Q5U4F0 |
| Gene Summary | Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR212043 | Rrp8 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 1500003O22 (RGD1308302), (10 ug) |
CNY 5,990.00 |
|
| RR212043L3 | Lenti ORF clone of Rrp8 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 1500003O22 (RGD1308302), (10 ug) |
CNY 7,790.00 |
|
| RR212043L4 | Lenti ORF clone of Rrp8 (mGFP-tagged ORF) - Rat similar to RIKEN cDNA 1500003O22 (RGD1308302), (10 ug) |
CNY 8,550.00 |
