Insr (NM_017071) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212026 representing NM_017071 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCGGGAGAGGATGTGAGACGACGGCGGTGCCGTTGCTGATGGCCGTGGCCGCGTTGCTGGTGG GCACAGCCGGCCACCTGTACCCTGGAGAGGTGTGCCCGGGAATGGACATCCGGAACAACCTGACCAGGTT GCATGAGCTGGAGAACTGCTCGGTCATTGAGGGCCATCTGCAGATCCTCTTGATGTTCAAGACCAGACCC GAAGATTTCCGAGACCTCAGTTTCCCCAAACTCATCATGATCACAGACTACCTGCTTCTTTTCCGTGTCT ATGGCCTAGAAAGTCTAAAAGACCTCTTCCCAAACCTCACAGTCATCCGAGGCTCCCGTCTCTTCTTCAA CTATGCCCTGGTCATCTTCGAGATGGTCCACCTGAAGGAGCTGGGTCTTTACAACCTCATGAACATCACC CGGGGCTCTGTCCGCATCGAGAAGAATAATGAACTCTGCTACCTGGCCACTATCGACTGGTCCCGCATCC TGGATTCCGTGGAGGACAACTACATTGTACTGAACAAAGATGACAATGAGGAATGTGGGGACGTCTGTCC AGGCACAGCCAAGGGCAAGACCAACTGCCCTGCCACCGTCATCAATGGGCAGTTTGTGGAACGGTGCTGG ACACACAGTCATTGTCAGAAAGTTTGCCCAACCATCTGTAAGTCACATGGCTGCACAGCTGAAGGCCTGT GCTGTCACAAGGAGTGCCTGGGCAACTGTTCAGAACCCGATGACCCTACCAAGTGTGTGGCCTGTCGAAA CTTCTACCTGGATGGTCAGTGTGTGGAGACCTGCCCACCACCCTACTATCACTTCCAGGATTGGCGCTGT GTAAACTTCAGCTTCTGCCAAGACCTTCACTACAAATGCAGGAACTCTCGGAAGCCTGGCTGTCACCAAT ACGTCATTCACAACAACAAGTGCATCCCCGAGTGCCCGTCTGGCTATACCATGAATTCCAGCAACTTGAT GTGCACCCCGTGTCTGGGACCCTGTCCTAAGGTCTGCCAAATCCTCGAAGGCGAGAAGACCATTGATTCC GTGACATCTGCCCAGGAGCTCCGAGGCTGCACTGTGATCAATGGCAGCCTGATTATCAACATCCGAGGGG GCAACAACCTGGCAGCTGAACTAGAGGCTAACCTTGGCCTCATTGAAGAAATCTCAGGGTTCCTAAAGAT CCGTCGCTCCTATGCTCTGGTGTCACTTTCTTTCTTCAGGAAGCTACATCTGATTCGAGGAGAGACCTTG GAAATTGGGAACTATTCTTTCTACGCCTTGGACAACCAGAACCTGAGGCAGCTGTGGGACTGGAACAAGC ACAACCTCACGATCACTCAGGGCAAGCTCTTCTTCCACTACAACCCCAAACTCTGCCTGTCTGAGATCCA CAAGATGGAGGAAGTGTCCGGAACCAAGGGCCGCCAGGAGAGGAACGACATTGCCCTGAAGACCAACGGG GACCAGGCATCCTGTGAAAATGAATTACTTAAATTCTCTTTCATCCGGACGTCTTTTGACAAGATCTTGC TGAGGTGGGAGCCCTACTGGCCCCCCGACTTCCGAGATCTCCTGGGATTCATGCTGTTCTACAAAGAGGC CCCTTATCAGAACGTGACAGAGTTTGACGGGCAGGATGCGTGTGGCTCCAACAGCTGGACTGTGGTGGAT ATTGACCCGCCCCAGAGGTCCAACGACCCTAAGTCTCAGACCCCAAGCCACCCTGGGTGGCTGATGCGGG GCCTCAAACCCTGGACCCAGTATGCCATCTTTGTCAAGACCTTGGTTACCTTCTCTGATGAACGCCGGAC CTATGGAGCCAAAAGTGATATCATCTATGTGCAAACAGATGCCACCAATCCTTCCGTTCCCCTGGATCCC ATATCAGTTTCCAACTCCTCATCTCAGATTATCTTAAAGTGGAAGCCCCCCTCGGACCCCAATGGCAACA TCACACACTACCTGGTCTACTGGGAGAGGCAGGCGGAGGACAGCGAGCTGTTCGAGTTGGATTATTGTCT CAAAGGGCTGAAGCTCCCCTCACGCACCTGGTCCCCACCTTTTGAGTCTGACGATTCTCAAAAGCACAAT CAGAGTGAGTATGACGACTCGGCCAGCGAGTGCTGCTCATGTCCTAAGACTGACTCTCAGATCCTGAAGG AGCTGGAGGAGTCTTCATTCAGGAAGACCTTCGAGGATTACCTGCACAACGTGGTTTTTGTTCCCAGAAA AACCTCTTCAGGCAATGGTGCTGAGGACACTAGGCCATCCCGAAAGCGAAGATCCCTTGAAGAGGTGGGC AATGTGACAGCCACTACACCCACACTTCCAGATTTTCCCAACATCTCCTCCACCATCGCGCCCACAAGCC ACGAAGAGCACAGACCATTTGAGAAAGTAGTAAACAAGGAGTCACTTGTCATCTCTGGCCTGAGACACTT CACTGGGTACCGCATTGAGCTGCAGGCATGCAATCAGGACTCCCCAGAAGAGAGGTGCAGCGTGGCTGCC TACGTCAGTGCCCGGACCATGCCTGAAGCTAAGGCAGATGACATCGTTGGCCCTGTGACCCATGAAATCT TTGAGAACAATGTTGTACACTTAATGTGGCAAGAGCCAAAGGAACCTAATGGTCTGATTGTGCTATATGA AGTGAGCTATCGGCGATATGGTGATGAGGAGCTGCACCTCTGTGTCTCCCGGAAGCATTTTGCCCTGGAG CGGGGCTGCAGGCTTCGAGGGCTCTCTCCAGGAAACTACAGTGTTCGAGTCCGGGCTACCTCTCTGGCAG GAAATGGCTCCTGGACAGAACCCACCTATTTTTATGTGACTGATTATTTAGATGTCCCATCAAATATTGC CAAAATTATCATCGGGCCCCTCATCTTCGTCTTCCTCTTCAGTGTCGTGATCGGAAGTATTTATCTATTC TTGAGGAAGAGGCAGCCAGATGGGCCAATGGGACCACTGTACGCTTCTTCAAACCCAGAGTACCTCAGTG CCAGTGATGTCTTTCCATCTTCCGTATACGTTCCGGATGAGTGGGAGGTACCTCGAGAGAAGATCACCCT CCTCCGAGAGCTGGGGCAGGGATCCTTCGGTATGGTGTACGAAGGCAATGCCAAGGATATCATCAAGGGT GAGGTAGAGACCCGTGTTGCGGTGAAGACGGTCAATGAGTCAGCCAGTCTTCGAGAACGGATCGAGTTCC TCAATGAGGCATCAGTCATGAAGGGCTTCACCTGTCATCACGTGGTCCGCCTTCTTGGGGTGGTGTCCAA AGGCCAGCCCACATTGGTAGTGATGGAACTGATGGCTCATGGAGACCTGAAAAGTCACCTCCGTTCTCTG CGGCCCGATGCTGAGAACAACCCAGGCCGTCCTCCCCCTACCTTGCAAGAAATGATTCAGATGACAGCAG AAATTGCCGATGGCATGGCATACTTGAACGCCAAGAAGTTTGTGCACCGGGACCTGGCAGCTCGGAACTG CATGGTTGCCCATGATTTTACTGTCAAAATCGGAGACTTTGGAATGACGAGAGACATCTACGAGACAGAT TACTATCGGAAAGGGGGCAAGGGGTTGCTGCCCGTGAGGTGGATGTCACCCGAGTCCCTGAAGGACGGAG TCTTCACTGCTTCTTCCGACATGTGGTCCTTTGGGGTGGTCCTTTGGGAAATCACCAGCCTGGCTGAGCA ACCTTACCAAGGCCTGTCTAATGAACAGGTGTTGAAATTTGTCATGGATGGAGGCTATCTGGATCCCCCT GATAACTGTCCAGAGAGACTCACTGACCTGATGCGCATGTGCTGGCAGTTCAACCCCAAGATGAGGCCGA CCTTCCTGGAAATCGTCAACCTGCTCAAGGACGACCTCCACCCCAGCTTTCCGGAAGTTTCCTTCTTCTA CAGCGAGGAGAACAAGGCTCCCGAGAGTGAAGAGCTGGAGATGGAGTTCGAGGACATGGAGAATGTCCCC TTGGATCGTTCCTCTCACTGTCAGAGAGAAGAGGCTGGATGCCGGGAGGGAGGGTCCTCTCTGAGCATCA AACGGACCTATGATGAACACATCCCCTACACCCACATGAACGGGGGCAAGAAGAATGGGCGGGTCCTCAC CCTGCCGAGGTCGAACCCTTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017071
Insert Size	4155 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017071.2, NP_058767.2
RefSeq Size	5399 bp
RefSeq ORF	4155 bp
Locus ID	24954
Gene Summary	receptor for insulin; involved in intracellular signalling; mutations in gene are associated with type 2 diabetes mellitus [RGD, Feb 2006]

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