Scn8a (NM_019266) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211948 representing NM_019266 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGCGGCTGCTCGCACCACCAGGCCCTGATAGTTTCAAGCCTTTCACCCCTGAGTCGCTGGCAA ACATCGAGAGGCGTATTGCCGAGAGCAAGCTCAAGAAACCACCAAAGGCGGATGGCAGCCACCGGGAGGA CGATGAAGACAGCAAGCCCAAGCCAAACAGTGACCTGGAGGCTGGGAAGAGTTTGCCTTTCATCTACGGG GACATCCCGCAAGGCCTGGTTGCGGTTCCCCTGGAGGACTTTGACCCTTACTATTTGACGCAGAAAACCT TTGTAGTATTAAACAGAGGGAAAACTCTCTTCAGATTTAGTGCCACACCTGCCTTGTACATTTTAAGCCC TTTTAACCTGATAAGAAGAATAGCTATTAAAATTTTGATACACTCAGTTTTCAGCATGATCATCATGTGC ACCATCCTGACCAACTGTGTGTTCATGACCTTTAGTAACCCTCCAGAATGGTCCAAGAATGTGGAGTACA CATTCACAGGGATTTACACATTTGAATCACTAGTGAAAATCATCGCAAGAGGTTTCTGCATAGACGGCTT CACCTTCTTGCGAGACCCGTGGAACTGGTTAGACTTCAGTGTCATCATGATGGCATATGTGACAGAGTTT GTGGACCTGGGCAATGTCTCAGCGCTGAGAACATTCAGGGTTCTCCGAGCTTTGAAAACTATCTCTGTAA TTCCAGGCCTGAAGACAATCGTGGGCGCCCTAATCCAGTCCGTGAAGAAGCTGTCGGACGTGATGATCCT GACAGTGTTCTGCCTGAGTGTTTTCGCCCTGATTGGCCTGCAGCTCTTCATGGGGAACCTTCGAAACAAG TGTGTCGTGTGGCCCATAAACTTCAACGAGAGCTACCTGGAGAACGGCACCAGAGGCTTTGACTGGGAGG AATATATCAACAATAAAACAAACTTTTACATGGTTCCTGGCATGCTAGAACCCTTGCTCTGCGGGAACAG TTCTGATGCTGGGCAATGCCCAGAGGGATTCCAGTGCATGAAAGCAGGAAGGAACCCCAACTACGGTTAC ACCAGCTTTGACACCTTCAGCTGGGCCTTCTTGGCATTATTCCGCCTTATGACCCAGGACTATTGGGAGA ACTTATACCAGCTGACCTTACGAGCCGCTGGGAAAACGTACATGATCTTCTTTGTCTTGGTCATCTTCGT GGGTTCTTTCTATCTGGTGAACTTGATCTTGGCTGTGGTGGCCATGGCTTATGAGGAACAGAACCAGGCA ACACTGGAGGAGGCAGAGCAAAAAGAGGCCGAGTTCAAGGCAATGCTGGAGCAACTCAAGAAGCAGCAGG AGGAGGCACAGGCTGCTGCAATGGCCACCTCAGCGGGCACTGTCTCGGAAGACGCCATTGAAGAAGAAGG GGAAGATGGGGTAGGCTCTCCGAGGAGCTCTTCTGAACTGTCTAAACTCAGTTCCAAGAGCGCGAAGGAG CGGCGGAACCGACGGAAGAAGAGGAAGCAGAAGGAGCTCTCTGAAGGCGAGGAGAAAGGGGACCCGGAGA AGGTGTTTAAGTCAGAGTCGGAAGACGGTATGAGAAGGAAGGCCTTCCGGCTGCCAGACAACAGGATAGG GAGGAAGTTTTCCATCATGAATCAGTCGCTGCTCAGCATTCCAGGCTCGCCCTTCCTCTCCCGACATAAC AGCAAAAGCAGCATCTTCAGCTTCCGGGGACCCGGTCGGTTCCGGGACCCCGGCTCTGAGAATGAGTTCG CAGACGATGAACACAGCACCGTGGAGGAGAGCGAGGGCCGGCGTGACTCGCTCTTCATCCCGATCCGCGC CCGCGAGCGCCGCAGCAGCTACAGTGGCTACAGCGGCTACAGCCAGTGCAGCCGCTCGTCGCGCATCTTC CCCAGCCTGCGGCGCAGCGTGAAGCGCAACAGCACGGTGGACTGCAACGGCGTAGTGTCACTCATCGGGC CCGGCTCACACATCGGGCGGCTCCTGCCTGAGGCAACGACTGAGGTGGAAATTAAGAAGAAAGGCCCTGG ATCTCTTTTAGTTTCTATGGACCAACTCGCCTCCTACGGACGGAAGGACAGAATCAACAGCATAATGAGC GTGGTCACAAACACGCTAGTGGAAGAGCTGGAAGAGTCTCAGAGAAAGTGCCCACCGTGCTGGTATAAGT TTGCCAACACTTTCCTCATCTGGGAGTGTCACCCCTACTGGATAAAACTGAAGGAGATCGTGAACTTAAT CGTCATGGACCCTTTTGTAGACTTAGCCATCACCATCTGCATCGTTCTGAATACGCTATTTATGGCAATG GAGCACCATCCCATGACACCACAGTTCGAACACGTCTTGGCCGTAGGAAATCTGGTGTTCACCGGGATCT TCACGGCGGAAATGTTTCTGAAGCTCATAGCCATGGACCCCTACTATTATTTCCAAGAAGGCTGGAACAT TTTTGACGGATTTATTGTCTCCCTCAGTTTAATGGAGCTGAGTCTCGCAGATGTGGAGGGGCTCTCAGTG CTGCGGTCTTTCCGACTGCTCCGAGTCTTCAAGCTGGCCAAGTCCTGGCCCACCCTGAACATGCTGATCA AGATCATCGGGAACTCCGTGGGTGCCCTGGGCAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTT CGCCGTGGTGGGGATGCAGCTGTTTGGAAAGAGTTACAAGGAGTGCGTCTGTAAGATCAACCAGGAGTGC AAGCTCCCGCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTCATCGTCTTCCGAGTGCTGTGTGGGG AGTGGATCGAGACCATGTGGGACTGCATGGAGGTGGCCGGCCAGGCCATGTGCCTCATTGTCTTCATGAT GGTTATGGTCATTGGCAACCTGGTGGTGCTGAATCTATTCCTGGCCTTGCTTCTGAGCTCCTTCAGCGCA GACAACCTGGCGGCCACAGACGACGACGGGGAAATGAACAACCTGCAGATCTCAGTGATCCGGATCAAGA AGGGCGTGGCCTGGACCAAAGTGAAGGTGCACGCCTTCATGCAGGCTCACTTCAAGCAGCGGGAGGCGGA TGAAGTGAAACCCCTCGACGAGCTGTATGAGAAGAAGGCCAACTGCATCGCCAACCACACGGGCGTGGAT ATCCACCGGAACGGCGACTTCCAGAAGAACGGGAACGGAACCACCAGCGGCATCGGCAGCAGCGTGGAGA AGTACATCATCGACGAGGACCACATGTCCTTCATTAACAACCCAAACCTGACCGTCCGGGTGCCCATTGC TGTGGGCGAGTCTGACTTCGAGAACCTCAACACAGAGGATGTTAGCAGCGAATCAGACCCTGAAGGCAGC AAAGATAAACTGGACGATACCAGCTCCTCAGAAGGAAGTACCATCGACATCAAGCCTGAGGTGGAAGAAG TTCCCGTGGAGCAACCTGAGGAATACTTGGATCCGGACGCCTGCTTTACAGAGGGTTGCGTCCAGCGGTT CAAGTGCTGCCAGGTCAACATCGAGGAAGGACTAGGCAAGTCGTGGTGGATCTTGCGGAAAACCTGCTTC CTCATTGTGGAGCACAATTGGTTTGAGACCTTCATCATCTTCATGATTCTGCTCAGCAGTGGCGCCCTGG CCTTTGAGGACATCTACATTGAGCAGAGGAAGACCATCCGCACCATCCTGGAGTATGCGGACAAGGTCTT CACCTACATCTTCATCCTGGAGATGTTGCTCAAGTGGACAGCCTACGGCTTCGTCAAGTTCTTCACCAAT GCCTGGTGCTGGTTGGACTTCCTCATTGTGGCTGTCTCTTTAGTCAGCCTTATAGCTAATGCCCTGGGCT ACTCGGAACTAGGTGCCATAAAGTCCCTTAGGACCCTAAGAGCTTTGAGACCCTTAAGAGCCTTATCACG ATTTGAAGGGATGAGGGTGGTGGTGAATGCCTTGGTGGGCGCCATCCCCTCCATCATGAATGTGCTGCTG GTGTGTCTCATCTTCTGGCTGATTTTCAGCATCATGGGAGTTAACCTGTTTGCGGGGAAATACCACTACT GCTTTAATGAGACTTCTGAAATCCGGTTCGAAATCGATATTGTCAACAATAAAACGGACTGTGAGAAGCT CATGGAGGGCAACAGCACGGAGATCCGATGGAAGAATGTCAAGATCAACTTTGACAATGTCGGAGCAGGG TACCTGGCCCTTCTTCAAGTGGCAACCTTCAAAGGCTGGATGGACATCATGTATGCGGCTGTAGATTCCC GAAAGCCAGACGAGCAGCCTGACTACGAGGGCAACATCTACATGTACATCTACTTCGTCATCTTCATCAT CTTCGGCTCCTTCTTCACCCTCAACCTGTTCATCGGTGTCATCATCGACAACTTCAACCAGCAGAAGAAA AAGTTTGGAGGTCAGGACATCTTCATGACAGAGGAACAGAAGAAGTACTACAATGCCATGAAAAAGCTGG GCTCCAAGAAGCCACAGAAGCCCATCCCCCGACCCTTGAACAAAATCCAAGGGATTGTCTTTGATTTCGT CACTCAACAAGCCTTTGACATTGTGATCATGATGCTCATCTGCCTTAACATGGTGACAATGATGGTGGAG ACAGACACTCAGAGCAAGCAGATGGAGAACATTCTTTACTGGATTAATCTGGTCTTTGTCATCTTCTTCA CCTGCGAGTGTGTGCTCAAAATGTTTGCCTTGAGACACTACTATTTCACCATTGGCTGGAACATCTTTGA CTTTGTGGTGGTCATCCTCTCCATTGTGGGAATGTTCCTGGCTGATATCATTGAGAAGTACTTCGTCTCC CCAACCCTATTCCGAGTTATCCGATTGGCCCGTATTGGGCGCATCTTGCGTCTGATCAAGGGCGCCAAAG GGATCCGCACCCTGCTCTTTGCCTTAATGATGTCGCTGCCCGCCCTGTTCAACATCGGCCTCCTGCTCTT CCTCGTCATGTTCATCTTCTCCATTTTTGGCATGTCCAACTTCGCATACGTGAAGCACGAGGCCGGCATT GACGACATGTTCAACTTCGAGACATTTGGCAACAGCATGATCTGTTTGTTCCAGATCACAACGTCTGCTG GCTGGGATGGCCTGCTGCTGCCAATCCTGAACCGCCCCCCTGACTGCAGCTTGGACAAAGAGCACCCAGG GAGTGGCTTCAAAGGGGACTGTGGGAACCCCTCGGTGGGCATCTTCTTCTTTGTGAGCTACATCATCATC TCCTTCCTGATTGTGGTGAACATGTACATCGCCATCATCCTGGAGAACTTCAGCGTGGCCACCGAGGAGA GCGCCGACCCTCTGAGTGAGGATGACTTCGAGACTTTCTATGAGATCTGGGAGAAGTTTGACCCAGACGC CACCCAGTTCATCGAGTACTGTAAGCTGGCAGACTTTGCCGACGCCCTGGAGCACCCGCTCCGAGTACCC AAGCCCAACACCATCGAGCTCATCGCCATGGACCTGCCCATGGTGAGCGGAGATCGCATCCACTGCTTGG ACATCCTTTTCGCCTTCACCAAGCGAGTCCTGGGAGACAGTGGGGAGTTGGACATCCTGCGGCAGCAGAT GGAGGAGCGGTTCGTGGCATCCAATCCTTCCAAAGTGTCTTACGAGCCTATCACAACCACTCTGCGGCGC AAGCAGGAGGAGGTGTCTGCAGTGGTCCTGCAGCGTGCCTACAGGGGACACTTGGCTAGGCGGGGCTTCA TCTGCAGAAAGATGGCCTCCAACAAGCTGGAGAATGGAGGCACACACAGAGACAAGAAGGAGAGCACCCC GTCCACAGCCTCCCTCCCCTCTTACGACAGCGTCACAAAGCCAGACAAGGAGAAGCAGCAGCGTGCGGAG GAGGGCAGAAGGGAAAGAGCCAAGAGGCAAAAAGAGGTCAGGGAGTCCAAGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019266
Insert Size	5937 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019266.2, NP_062139.2
RefSeq Size	6556 bp
RefSeq ORF	5937 bp
Locus ID	29710
UniProt ID	O88420
Gene Summary	tetrodotoxin-sensitive sodium channel alpha subunit [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.