Nrcam (NM_013150) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211835 representing NM_013150 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGAAGAAGCCCTTGTCTGCAGGCAGAGCGCCCCTGTTTCTCTTCCTGTGCCAGATGATCAGCG CTCTGGATGTTCCTCTTGATCCAAAGCTCCTTGATGACTTGGTACAGCCTCCAACTATCACTCAACAGTC ACCAAAAGACTACATCATTGACCCACGGGAGAATATTGTAATCCAATGTGAGGCCAAAGGGAAACCTCCT CCAAGCTTTTCCTGGACTCGTAACGGAACACATTTTGACATAGACAAAGACCCTCTGGTCACTATGAAGC CTGGCTCAGGAACCCTTGTCATCAACATCATGAGTGAAGGAAAGGCGGAGACCTATGAAGGGGTTTACCA GTGCACTGCAAGGAATGAGCGCGGAGCTGCTGTCTCCAATAACATTGTTGTCCGCCCCTCTAGGTCACCC TTGTGGACCAAGGAAAGACTTGAACCAATAATCCTCCGAAGTGGTCAGTCACTAGTACTACCATGTAGGC CTCCAATTGGATTACCACCGGCCATAATATTTTGGATGGATAACTCCTTTCAAAGACTGCCACAGAGTGA GCGGGTTTCCCAAGGACTGAATGGAGACCTTTACTTCTCCAATGTCCTCCCAGAGGACACCCGTGAGGAC TACATCTGCTATGCCAGATTTAATCACACTCAAACAATTCAACAGAAACAACCTATTTCTCTGAAGGTGA TTTCAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCTAAATC TAGTAAAGAGAGGCCACCAACATTTCTAACTCCAGAGGGCAATGAAAGTCACAAGGAAGAATTAAGAGGA AACGTGCTTTCCCTGGAGTGCATTGCAGAAGGCCTACCTACTCCAGTTATTTACTGGATCAAGGAAGATG GAACGCTTCCTGTCAACCGGACGTTTTATCGGAACTTTAAGAAAACCTTGCAGATCATTCATGTCTCTGA AGCAGACTCTGGAAATTATCAGTGCATAGCAAAAAACGCATTGGGAGCCGTCCATCATACCATTTCTGTC ACAGTTAAAGCGGCTCCCTACTGGATTGTTGCACCTCACAACCTCGTGCTTTCCCCAGGGGAGAATGGGA CCCTCATCTGCAGAGCTAACGGCAACCCAAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCAGTAGA AATTGCTCTCGATGACCCCAGCCGAAAAATCGATGGTGATACCATTATGTTTTCAAATGTTCAAGAAAGC TCAAGTGCGGTTTATCAGTGCAATGCCTCTAACAAATATGGATATTTACTAGCAAATGCATTTGTAAATG TGCTCGCTGAACCACCTCGGATTCTTACCTCAGCAAACACACTGTACCAGGTCATTGCAAACCGGCCTGC TTTGCTGGACTGTGCCTTCTTTGGGTCTCCTATGCCTACCATTGAGTGGTTTAAAGGAACTAAAGGAAGC GCTCTTCACGAAGACATTTATGTTTTACATGATAACGGAACGCTAGAAATTCCTGTGGCCCAAAAGGATA GTACAGGGACATATACTTGTGTCGCAAGGAATAAATTAGGGATGGCAAAGAATGAAGTTCACTTGGAAAT CAAAGATCCAACCAGGTTCATTAAACAACCTGAATATGCAGTCGTTCAGAGGGGGAGCAAGGTTTCCTTT GAATGCAAAGTGAAACACGACCACACCTTAATCCCCACCATTCTGTGGCTGAAGGACAATGGAGAGCTGC CCAATGATGAAAGGTTCTCTGTCGACAAGGACCATCTGGTGGTCTCTGACGTAAAGGATGAAGATGGTGG GACCTACACATGTGCAGCCAACACAACGCTGGACAGTGTTTCAGCCAGCGCCGTGCTCAGGGTGGTCGCT CCTACTCCAACTCCAGCCCCCATTTACGATGTCCCGAATCCTCCCTTTGATTTAGAATTGACCAATCAAC TTGACAAAAGTGTTCAGCTGACATGGACCCCAGGCGATGACAACAATAGTCCCATTACAAAATTCATCAT CGAGTATGAAGATGCAATGCATGAGGCAGGGCTGTGGCGCCACCAGGCGGAAGTTTCTGGAACGCAGACC ACAGCCCAGCTGAAGCTGTCTCCCTATGTGAACTACTCCTTCCGAGTCATGGCGGAGAACAGCATCGGGA GGAGTGTGCCAAGCGAGGCATCGGAGCAGTATCTTACAAAGGCCGCGGAACCAGACCAGAATCCCACGGC TGTGGAAGGACTAGGGACAGAGCCGGACAACTTGGTGATCACATGGAAGCCCCTGAATGGTTTTCAATCG AATGGGCCCGGCCTCCAGTACAAAGTGAGCTGGCGTCAGAAAGATGGTGACGATGAATGGACGTCTGTGG TTGTGGCCAATGTATCCAAATACATTGTTTCTGGCACACCAACTTTTGTCCCATACCTGATAAAAGTTCA AGCCCTGAATGATGTGGGCTTTGCTCCAGAGCCAGCTGCAGTCATGGGACATTCCGGAGAAGACCTTCCA ATGGTGGCTCCTGGAAATGTGCGTGTCAGTGTGGTGAACAGCACCTTGGCAGAGGCACACTGGGACCCTG TACCTCCCAAGAGTGTCCGAGGACACTTACAAGGCTACCGGATTTACTACTGGAAGGCTCAGAGCTCATC TAAAAGAAACAGACGCCACATTGAGAAGAAGATCCTCACCTTCCAGGGCAGCAAGACTCACGGCATGCTG CCAGGGCTGCAACCTTACAGTCACTATGTTCTCAATGTCCGAGTGGTCAATGGGAAAGGGGAGGGCCCAG CCAGCGCCGACAGAGGCTTCCATACTCCAGAGGGAGTCCCTAGTGCTCCCTCATCTTTGAAAATTGTTAA TCCTACACTGGACTCTCTCACTTTGGAATGGGACCCTCCAAGCCACCCAAATGGTATCCTGACTGAATAC ATCTTAAAATATCAGCCAATTAATAGCACACACGAACTAGGCCCTCTGGTAGATTTAAAAATTCCTGCCA ACAAGACACGCTGGACTTTGAAAAATTTAAATTTCAGCACTCGGTACAAGTTCTACTTCTATGCACAAAC ATCAGTGGGATCGGGCAGTCAGATCACAGAGGAAGCAATAACAACTGTGGACGAAGCTGGTATTCTTCCA CCTGATGTAGGTGCAGGCAAAGTCCGTGCAGTAAGTCCCAGGATCGGCAATGTCACTGCTGCAGCTGCTG AGACGTATGCCAATATCAGTTGGGAATATGAGGGACCAGAGCATGTGAAGTTTTATGTTGAATATGGTGT AGCAGGCAGCAAAGAAGAATGGAGGAAAGAGATTGTAAATGGTTCTCGGAGCTTCTTTGGGTTAAAGGGT CTAATGCCAGGAACAGCATACAAAGTCCGAGTTGGTGCTGAGGGGGACTCTGGTTTTGTGAGTTCAGAGG ATGTGTTTGAGACAGGACCAGCAATGGCAAGTCGGCAAGTGGATATTGCGACCCAAGGCTGGTTCATAGG TCTAATGTGTGCTGTTGCCCTCCTCATCTTAATTTTGCTAATCGTTTGCTTCATCAGAAGAAACAAAGGT GGTAAATATCCAGTTAAAGAAAAGGAGGATGCTCACGCAGACCCTGAAATCCAGCCCATGAAGGAGGATG ACGGAACGTTTGGAGAATATAGTGATGCAGAAGATCACAAGCCTTTGAAAAAAGGAAGTCGAACACCTTC AGACAGGACTGTGAAAAAAGAAGATAGTGATGACAGCCTGGTTGACTATGGCGAGGGGGTGAATGGCCAA TTCAATGAGGATGGCTCCTTTATCGGACAATACAGTGGTAAGAAAGAGAAAGAACCAGCAGAGGGAAATG AAAGCTCAGAGGCCCCTTCTCCTGTCAACGCAATGAACTCCTTTGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013150
Insert Size	3900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013150.1, NP_037282.1
RefSeq Size	7556 bp
RefSeq ORF	3900 bp
Locus ID	497815
Gene Summary	a neuronal cell adhesion molecule localized at the node of Ranvier of myelinated axons [RGD, Feb 2006]

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