Dcx (NM_053379) Rat Untagged Clone

CAT#: RN211526

Dcx (untagged ORF) - Rat doublecortin (Dcx), (10 ug)



  "NM_053379" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211526 representing NM_053379
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACTTGATTTTGGACATTTTGACGAACGAGACAAAGCATCTAGAAATATGAGAGGGTCACGGATGA
ATGGACTTCCAAGTCCCACTCATAGTGCTCACTGTAGCTTCTACAGAACCAGAACCTTGCAGGCATTAAG
TAATGAGAAGAAGGCCAAGAAGGTACGTTTCTACCGCAATGGGGACCGTTACTTCAAGGGGATTGTGTAC
GCTGTTTCTTCTGACCGTTTTCGTAGTTTTGATGCATTGCTGGCTGACCTGACTCGATCCTTGTCCGACA
ACATCAACCTGCCTCAGGGAGTGCGCTACATTTACACTATTGATGGATCCAGAAAGATTGGAAGCATGGA
TGAACTGGAAGAAGGGGAAAGCTATGTCTGTTCCTCAGACAACTTCTTTAAAAAGGTTGAGTATACCAAG
AATGTCAATCCCAACTGGTCGGTCAATGTAAAGACATCAGCCAACATGAAAGCGCCCCAGTCCTTGGCTA
GCAGCAACAGTGCTCAAGCCAGAGAGAACAAGGACTTCGTGCGCCCCAAACTTGTGACCATCATTCGCAG
TGGGGTGAAGCCTCGGAAGGCGGTGCGTGTGCTTCTCAACAAGAAAACAGCCCACTCTTTCGAGCAGGTC
CTCACTGACATCACAGAAGCGATCAAACTGGAAACCGGAGTTGTCAAAAAACTCTATACCCTTGATGGAA
AGCAGGTCACCTGTCTCCATGATTTCTTTGGTGATGATGATGTGTTCATTGCTTGTGGCCCTGAAAAGTT
CCGCTATGCTCAAGATGACTTCTCCTTGGATGAGAATGAATGCAGAGTCATGAAAGGGAATCCATCTGCC
ACAGCTGGCCCAAAAGCTTCCCCAACACCTCAAAAGACATCTGCTAAAAGCCCAGGCCCAATGCGCCGCA
GCAAGTCTCCAGCTGACTCAGGTAACGACCAAGACGCAAATGGAACTTCTAGCAGTCAGCTCTCAACACC
TAAGTCGAAGCAGTCTCCTATCTCTACACCCACAAGCCCTGGAAGTCTGCGGAAGCACAAGGACTTGTAC
CTGCCACTGTCATTGGATGACTCTGATTCACTTGGCGATTCCATGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053379
Insert Size 1098 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053379.3, NP_445831.3
RefSeq Size 8827 bp
RefSeq ORF 1098 bp
Locus ID 84394
Gene Summary This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. Studies in knockout mice lacking this gene suggest that this gene has a cortical role in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. This gene is essential for neuronal migration, differentiation, and plasticity, is required for hippocampal development and also plays a role in dendrites development.[provided by RefSeq, Sep 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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