Ppm1a (NM_017038) Rat Untagged Clone

CAT#: RN211341

Ppm1a (untagged ORF) - Rat protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), (10 ug)



  "NM_017038" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Pp2c1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211341 representing NM_017038
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCATTTTTAGACAAGCCAAAGATGGAAAAGCATAATGCCCAGGGCCAGGGGAACGGGTTGCGAT
ACGGCCTAAGCAGCATGCAAGGTTGGCGAGTTGAAATGGAGGATGCACATACGGCTGTGATCGGTTTGCC
AAGTGGACTTGAGACATGGTCATTCTTTGCTGTATATGATGGGCATGCTGGTTCTCAGGTTGCCAAATAC
TGCTGTGAGCACTTGTTAGATCACATCACCAATAACCAGGATTTTAAAGGCTCTGCAGGAGCACCCTCTG
TGGAAAACGTAAAGAACGGAATCAGAACAGGTTTTCTGGAGATTGATGAACACATGAGAGTTATGTCAGA
GAAGAAACACGGTGCAGATAGAAGTGGGTCAACAGCTGTGGGTGTCTTAATTTCTCCCCAACATACTTAT
TTCATTAACTGTGGGGACTCAAGAGGTTTACTTTGTAGAAATAGAAAAGTTCACTTCTTCACTCAAGACC
ATAAACCAAGTAATCCTCTGGAAAAAGAACGAATTCAGAATGCAGGGGGCTCTGTGATGATTCAGCGTGT
CAATGGCTCTCTGGCTGTATCAAGAGCTCTTGGGGATTTTGATTACAAATGTGTCCATGGAAAAGGTCCC
ACAGAGCAGCTTGTCTCGCCAGAGCCTGAAGTCCATGATATTGAAAGGTCTGAAGAAGATGACCAGTTCA
TCATTCTTGCATGTGATGGCATCTGGGACGTCATGGGCAACGAAGAGCTCTGTGACTTTGTGAGATCCAG
ACTTGAAGTCACTGATGACCTTGAGAAAGTTTGCAATGAAGTAGTCGACACCTGTTTGTATAAGGGAAGT
CGAGACAACATGAGTGTGATTTTGATCTGCTTTCCAAACGCGCCTAAAGTCTCAGCAGAGGCGGTGAAGA
AGGAGGCAGAGCTGGACAAGTACCTGGAGAACAGAGTAGAAGAAATCATAAAGAAGCAGGGGGAAGGCGT
CCCTGACTTAGTCCATGTGATGCGCACGTTAGCCAGTGAGAACATCCCCAGCCTCCCACCAGGGGGTGAA
CTGGCAAGCAAGCGGAATGTAATTGAAGCCGTTTACAATAGACTGAACCCTTACAAAAACGACGACACTG
ATTCTGCGTCAACTGATGATATGTGGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_017038
Insert Size 1149 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017038.1, NP_058734.1
RefSeq Size 1602 bp
RefSeq ORF 1149 bp
Locus ID 24666
UniProt ID P20650
Gene Summary Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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