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Abhd12 (NM_001024314) Rat Untagged Clone
CAT＃: RN211309
Abhd12 (untagged ORF) - Rat abhydrolase domain containing 12 (Abhd12), (10 ug)
CN¥ 5,488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN211309 representing NM_001024314
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAGCGGACCGAGCCCGTCACCTTGGAGCATGAGCGCTGCGCCGCCTCAGGCTCGTCTTCCTCCG GCTCGGCCGCCGCGGCGCTGGACGCCGACTGCAGCTTGAAGCAGAACCTGCGTCTGGCGGGCAAGGGGAC GGCAGAGCCGCACAGCGCATCCGACGCGGGCATGAAGCGGGCTCTGGGCAGACGGAAGAGCCTGTGGTTC CGACTAAGGAAGATACTTCTCTGTGTTTTGGGGTTCTACATTGCCATTCCATTTCTTGTCAAACTGTGTC CTGGGATACAGGCCAAACTGATATTCTTAAATTTCGTGAGGGTTCCCTATTTCATTGACTTAAAAAAGCC ACAGGATCAAGGTTTGAATCACACCTGCAATTACTACCTCCAGCCCGAGGATGATGTCACTATTGGAGTC TGGCACACCATTCCCTCTGTCTGGTGGAAGAATGCCCAAGGGAAGGACCAGATGTGGTATGAGGATGCTC TGGCTTCTAACCACCCCATCATCCTGTACCTGCATGGGAATGCAGGCACCAGAGGAGGTGACCACCGTGT GGAGCTGTACAAGGTGCTGAGTTCCCTTGGTTACCACGTGGTCACCTTCGACTACAGAGGTTGGGGTGAC TCAGTAGGAACACCATCAGAGCGAGGCATGACATATGATGCACTCCATGTTTTTGACTGGATCAAAGCAA GAAGTGGTGATAATCCTGTGTATATCTGGGGCCATTCGCTGGGCACTGGAGTGGCAACAAATCTGGTCCG GCGCCTTTGTGAGCGAGAGACGCCACCAGATGCCCTTATATTGGAGTCTCCGTTCACAAATATTCGTGAA GAAGCAAAGAGTCATCCATTTTCAGTGATATACAGATACTTCCCAGGCTTTGACTGGTTCTTCCTCGACC CCATTACAAGCAGTGGAATTAAATTTGCAAATGACGAAAACATGAAGCACATCTCCTGTCCCCTGCTCAT CTTGCACGCTGAGGATGACCCAGTTGTACCCTTTCATCTCGGCAGGAAGCTATACAATATTGCTGCGCCA TCCCGAAGTTTCCGAGACTTCAAAGTCCAGTTCATCCCCTTTCACTCAGACCTTGGCTACAGACATAAGT ACATCTACAAGAGCCCAGAGCTTCCACGGATACTGAGGGAATTCCTAGGGAAGTCGGAACCAGAGCGCCA GCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024314 |
| Insert Size | 1197 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024314.1, NP_001019485.1 |
| RefSeq Size | 1982 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 499913 |
| UniProt ID | Q6AYT7 |
| Gene Summary | Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes (By similarity). Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system (By similarity). Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR211309 | Abhd12 (Myc-DDK-tagged ORF) - Rat abhydrolase domain containing 12 (Abhd12), (10 ug) |
CN¥ 3,990.00 |
|
| RR211309L3 | Lenti ORF clone of Abhd12 (Myc-DDK-tagged ORF) - Rat abhydrolase domain containing 12 (Abhd12), (10 ug) |
CN¥ 6,080.00 |
|
| RR211309L4 | Lenti ORF clone of Abhd12 (mGFP-tagged ORF) - Rat abhydrolase domain containing 12 (Abhd12), (10 ug) |
CN¥ 6,650.00 |
