Fbn2 (NM_031826) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211125 representing NM_031826 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGAAGACGGAGGCTGTGTCTCCGGCCCTACTTCGTGTGGCTGGGTTGCGTGGCGCTCTGGGCGC AGGGCACAGATGGCCAGCCCCAGCCTCCTCCACCAAAGACGCTCCGGCCCCAGCCGCCCCCGCAACAGGT CCGGCCCGCAGGAGCGTCCGAAGGTGGCTTCGCGGGGCCTGAGTACAGGGACGAGGGCGCGTTAGCCGCG AGCCGGGTCCGCAGGCGAGGACAGCAGGAAATACTCCGAGGGCCCAACGTGTGCGGTTCTAGATTCCACT CCTATTGCTGCCCGGGATGGAAGACGCTCCCAGGAGGAAATCAGTGCATTGTCCCCATTTGCAGGAACAG CTGTGGAGATGGATTCTGTTCCCGCCCCAACATGTGTACCTGTTCCAGCGGACAAATATCCCCGACCTGT GGAGGCAAATCAATTCAGCAGTGTAGTGTGAGGTGCATGAACGGTGGGACCTGTGCGGATGACCACTGTC AGTGCCAGAAGGGATACATCGGGACCTACTGTGGACAACCTGTCTGTGAGACTGGATGCCAGAATGGTGG GCGTTGCATCGGCCCTAACCGCTGTGCTTGTGTTTATGGATTCACCGGGCCACAGTGCGAAAGAGATTAC AGGACAGGCCCATGTTTCACTCAAGTCAATAATCAGATGTGCCAGGGGCAGCTGACAGGCATCGTCTGTA CGAAGACGCTGTGCTGTGCCACCATTGGGCGAGCCTGGGGCCATCCTTGTGAGATGTGCCCAGCCCAGCC TCAGCCCTGCCGCCGGGGCTTCATCCCCAACATCCGCACTGGAGCATGTCAGGATGTTGACGAATGCCAG GCGATTCCAGGATTGTGCCAGGGCGGAAACTGTATCAACACAGTGGGCTCTTTTGAATGCAGATGCCCTG CTGGCCACAAGCAGAGTGAAACCACACAGAAATGTGAAGACATCGACGAGTGCAGCGTGGTTCCTGGAAT CTGTGAGACAGGCGACTGTTCCAACACCGTGGGAAGCTATTTTTGTCTCTGTCCACGCGGCTTCGTTACC TCAACAGATGGCTCCCGGTGCATTGATCAGAGAACTGGCACATGCTTCTCCGGCCTGGTGAATGGCCGCT GTGCTCAAGAACTCCCAGGCAGAATGGCAAAAGCTCAGTGCTGTTGCGAGCCCGGCCGTTGCTGGGGCAT CGGGACCATTCCTGAGGCCTGTCCTGTCAGAGGTTCTGAAGAGTACCGTAGACTGTGCCTGGATGGTCTT CCAATGGGCGGTATTCCGGGGAGTTCAGTTTCTAGACCTGGAGGGTCTGGGAGCAACAGCAATGGTTATG GCCCAGGAGGAACAGGCTTCCTGCCCATCCCTGGAGGCAATGGCTTTTCTCCTGGTGTTGGAGGAGCTGG AGTAGGAGCTGGAGGGCAGGGACCCATCATCACGGGACTAACAATTCTCAACCAGACAATAGACATCTGT AAGCACCATGCCAATCTGTGTTTGAACGGACGTTGCATACCTACTGTTTCCAGCTACAGATGCGAATGTA ACATGGGCTACAAGCAGGATGCTAATGGAGATTGTATAGATGTTGATGAATGTACATCAAACCCCTGCAG TCATGGAGATTGTGTTAACACACCTGGATCCTATTACTGTAAATGTCACGCTGGATTCCAGAGGACTCCT ACGAAGCAAGCATGTATCGACATTGATGAGTGCATCCAGAATGGGGTTCTGTGTAAAAATGGCCGATGCG TGAACACAGATGGCAGCTTCCAGTGCATTTGCAATGCTGGCTTTGAGTTAACCACAGATGGAGAAAACTG TGTTGGCCACGATGAATGTACAACGACCAACATGTGCTTGAACGGGATGTGTATCAATGAAGATGGCAGC TTCAAGTGCGTGTGCAAGCCAGGCTTTGTCCTGGCTCCAAATGGCCGCTGCTGCACAGACGTGGACGAGT GCCAGACCCCGGGCATCTGCATGAATGGGCACTGCATCAACAACGAAGGGTCCTTCCGCTGTGACTGTCC TCCCGGCCTGGCTGTGGGTGTGGATGGACGTGTATGTGTTGACACGCATATGCGTAGCACCTGCTATGGG GAAATCAAGAAAGGAGTGTGTGTGCGGCCATTCCCGGGTGCAGTGACCAAGTATGAATGCTGCTGTGCTA ATCCAGACTATGGTTTTGGAGAGCCCTGCCAGCCTTGTCCTGCTAAAAATTCAGCGGAGTTCCATGGTCT TTGTAGCGGTGGCGTAGGCATCACTGTGGATGGAAGAGATATCAATGAGTGTGCTTTGGATCCTGACATA TGTGCCAACGGGATCTGTGAAAACTTGCGAGGCAGCTACCGGTGTAACTGCAACAGTGGCTATGAGCCAG ACGCCTCAGGAAGAAATTGCATTGACATTGATGAGTGCTTGGTAAACAGGCTGCTTTGTGACAATGGCCT ATGCAGAAATACGCCTGGCAGTTACAGCTGCACGTGTCCCCCTGGCTATGTGTTCCGGACAGAGACAGAG ACCTGCGAAGATGTCAATGAGTGTGAAAGCAACCCGTGTGTCAATGGAGCCTGCAGGAACAACCTGGGCT CTTTCCACTGCGAGTGCTCGCCTGGGAGCAAGCTCAGCTCCACGGGACTGATCTGCATCGGCAGCCTGAA AGGGACCTGCTGGCTCAACATCCAGGACAACCGCTGCGAAGTGAACATTAACGGAGCCACCCTGAAATCT GAATGCTGCGCCACGCTTGGAACTGCCTGGGGGAGCCCCTGTGAGCGCTGCGAGCTAGATGCAGCTTGCC CGAGAGGGTTCGCCAGGATCAAAGGTGTGACCTGCGAAGACGTTAATGAGTGCGAGGTGTTTCCTGGCGT CTGTCCAAATGGACGCTGTGTCAACAACAAAGGCTCTTTCCACTGTGAATGCCCTGAAGGCCTTACTCTG GATGGCACAGGCCGGGTGTGTCTGGATGTCCGCATGGAGCACTGCTTCCTGAAGTGGGATGAGGATGAGT GCGTCCACCCCGTCCCCGGGAAGTTCCGCATGGACGCCTGCTGCTGTGCGGTCGGGGCGGCTTGGGGCAC AGAGTGTGAAGAGTGTCCAAAACCTGGCACCAAGGAATATGAGACCCTGTGTCCCCGGGGCCCCGGCTTT GCTAACAGAGGGGACATCCTCACTGGGCGTCCATTTTACAAAGATATCAACGAGTGCAAGGCCTTGCCTG GGATGTGCACCTATGGGAAGTGTAGAAACACAATCGGAAGCTTCAAGTGTCGTTGCAACAGTGGCTTTGC CCTGGACATGGAGGAGAGGAACTGTACTGACATTGATGAGTGCAGGATCTCCCCTGACCTCTGTGGCAAT GGCATCTGTGTCAACACGCCAGGCAGCTTTGAGTGCGAGTGCTTTGAGGGCTACGAGAGCGGCTTCATGA TGATGAAGAATTGCATGGACATTGACGAATGTGAACGTAACCCTCTCCTTTGTAGGGGTGGCACCTGTGT GAACACTGAGGGAAGCTTTCAGTGTGACTGCCCACTGGGACACGAGCTGTCGCCGTCACGCGAGGACTGC ATAGATATTAACGAGTGCTCCCTGAGCGACAATCTCTGCAGAAATGGCAAATGTGTGAACATGATCGGAA CCTACCAGTGTTCTTGCAATCCTGGATACCAGGCCACGCCAGATCGCCAGGGCTGTTCAGATATCGATGA GTGTATGATAATGAACGGGGGCTGCGACACCCAGTGCACCAACTCAGAAGGAAGCTACGAATGCAGCTGC AGCGAAGGGTACGCACTGATGCCAGACGGACGGTCGTGCGCAGATATTGACGAGTGTGAAAACAACCCCG ACATCTGTGACGGCGGCCAGTGTACCAACATTCCGGGGGAGTATCGCTGCCTCTGCTACGATGGCTTCAT GGCGTCGATGGACATGAAGACATGCATCGATGTGAACGAGTGTGACCTGAATCCCAACATCTGCATGTTT GGGGAGTGTGAGAACACAAAGGGCTCCTTCATTTGCCACTGCCAGCTGGGCTACTCAGTGAAGAAGGGGG CCACGGGATGCACAGACGTGGACGAGTGTGAAATTGGCGCCCACAACTGTGACATGCACGCCTCGTGCCT GAATGTCCCTGGGAGCTTCAAATGCAGCTGCAGAGAAGGCTGGGTGGGAAATGGCATCAAATGCATTGAT CTGGATGAATGCGCGAATGGAACCCACCAGTGCAGCATAAACGCCCAGTGTGTCAACACCCCAGGCTCGT ACCGATGTGCCTGCTCGGAGGGTTTCACGGGAGACGGCTTTACTTGCTCAGACGTTGATGAGTGCGCGGA AAACATAAACCTTTGTGAGAATGGCCAGTGCCTGAACGTCCCGGGAGCTTACCGCTGCGAATGTGAGATG GGCTTCACCCCAGCCTCAGACAGCAGGTCCTGCCAAGATATTGACGAATGCTCCTTCCAAAATATCTGCG TCTTCGGGACATGCAACAACTTGCCCGGGATGTTTCACTGCATCTGTGACGATGGTTACGGACTGGACAG AACAGGAGGACACTGCACAGATATTGACGAGTGCGCAGATCCCATAAACTGTGTCAATGGCCTATGCGTG AACACACCTGGTCGCTATGAGTGTAACTGCCCGCCTGACTTTCAGCTGAACGCCACTGGTGTGGGCTGCG TTGACAACCGAGTGGGCAACTGTTACCTGAAGTTTGGTCCTCGAGGAGATGGGAGTCTGTCCTGCAAGAC AGAGGTCGGGGTGGGTGTCAGTTGCTCCTCCTGCTGCTGCTCCCTGGGGAAGGCCTGGGGAAACCCCTGT GAGACGTGCCCCCCTGTAAACAGCACTGAGTACTATAGCCTGTGTCCTGGTGGGGAAGGCTTTAGGCCGA ACCAGATCACGATCATCTTAGAAGACATTGACGAATGTCAGGAGTTACCAGGGCTCTGCCAGGGTGGAAA CTGTATCAACACTTTCGGGAGCTTCCAGTGTGAGTGTCCACAGGGTTACTACCTCAGTGAAGAAACCCGC ATCTGTGAAGATATTGATGAGTGCTTCGCCCATCCCGGCGTGTGCGGACCTGGAACCTGCTACAACACTC TGGGGAATTACACTTGCATTTGCCCGCCCGAGTACATGCAAGTCAACGGAGGACACAACTGCATGGACAT GAGGAAAAGCTTTTGCTACAGAAGCTATAACGGGACCACTTGTGAAAACGAGTTGCCTTTTAATGTGACA AAGAGGATGTGCTGCTGCACGTACAACGTCGGCAAGGCCTGGAACAAACCTTGTGAGCCATGCCCTACGC CGGGGACAGCTGACTTTAAAACTATATGTGGAAATATTCCCGGATTCACCTTTGACATTCACACAGGAAA AGCTGTTGACATTGACGAGTGTAAAGAGATTCCGGGCATCTGTGCAAACGGTGTGTGCATTAACCAGATC GGCAGTTTCCGCTGTGAATGCCCCACAGGCTTCAGTTACAACGACCTGCTCTTGGTCTGTGAAGACATCG ATGAGTGCAGCAATGGTGACAACCTGTGCCAACGAAACGCGGACTGCATCAACAGCCCAGGCAGCTACCG CTGCGAGTGTGCGGCGGGCTTCAAGCTCTCCCCCAACGGGGCCTGTGTAGATCGCAATGAGTGTTTGGAG ATTCCTAATGTTTGCAGTCATGGGCTGTGTGTTGATTTGCAAGGAAGTTACCAGTGCATCTGCAACAACG GCTTCAAGGCGTCTCAAGACCAGACCATGTGCATGGATGTTGATGAGTGTGAGAGGCACCCATGTGGAAA CGGGACTTGTAAGAACACTGTCGGGTCCTACAACTGTCTGTGCTACCCTGGCTTCGAGCTCACCCACAAC AACGACTGCCTAGACATAGACGAGTGCAGCTCGTTCTTTGGCCAGGTGTGCAGAAACGGGCGGTGCTTCA ATGAAATCGGCTCCTTCAAATGTCTGTGCAACGAGGGCTACGAACTTACTCCGGATGGCAAGAACTGTAT CGATACTAATGAGTGTGTCGCACTTCCTGGCTCCTGCTCCCCTGGGACCTGTCAGAATTTGGAGGGATCC TTCAGGTGCATCTGCCCCCCTGGATATGAAGTGAAAAGCGAAAATTGCATTGATATAAATGAATGTGACG AGGACCCCAACATTTGCCTCTTTAGTTCCTGCACCAACACCCCAGGGGGCTTCCAATGCATCTGTCCCCC TGGCTTTGTCTTGTCTGACAACGGACGGAGATGCTTCGATACCCGCCAGAGCTTCTGCTTCACCAACTTT GAAAACGGAAAGTGTTCTGTACCCAAAGCCTTCAACACCACCAAAGCAAAATGCTGTTGCAGCAAGATGC CAGGCGAGGGCTGGGGGGACCCGTGTGAGCTGTGCCCCAAAGACGATGAAGTTGCCTTCCAGGATCTGTG TCCATATGGCCATGGGACAGTCCCTAGTCTTCATGACACACGTGAAGATGTCAATGAGTGCCTTGAGAGC CCAGGCATTTGCTCAAACGGTCAGTGCATTAACACTGACGGATCCTTCCGATGTGAGTGTCCGATGGGCT ACAATCTGGATTACAGTGGAGTCCGGTGTGTGGACACTGATGAGTGTTCGATCGGTAATCCCTGTGGGAA CGGGACCTGCGCCAACGTGATCGGGAGTTTCGAATGCAATTGCAATGAGGGCTTTGAGCCGGGGCCCATG ATGAACTGTGAAGACATCAACGAGTGTGCCCAGAACCCGCTGTTCTGCGCTTTCCGCTGCATGAACACCT TTGGATCCTATGAATGCACATGCCCAGTTGGCTACGCCCTCAGGGAAGACCAAAAGATGTGTAAAGATCT GGATGAATGCGCTGAGGGGCTTCACGACTGTGAGTCCAGAGGCATGATGTGTAAGAATCTGATCGGCACC TTCATGTGCATCTGCCCCCCTGGCATGGCCCGCAGGCCAGACGGAGAAGGCTGCGTAGACGAAAACGAAT GCAGGACCAAGCCCGGCATCTGTGAGAATGGACGCTGCGTTAACATCATTGGAAGCTACAGGTGTGAGTG CAACGAAGGATTCCAGTCAAGCTCCTCGGGCACCGAATGCCTTGACAACCGACAGGGCCTGTGCTTCGCA GAGGTGCTGCAGACAATGTGTCAGATGGCCTCCAGTAGCCGTAACCTCGTCACAAAGTCAGAATGCTGTT GTGATGGCGGGCGAGGCTGGGGCCACCAGTGTGAGCTCTGCCCACTTCCTGGAACTGCCCAGTACAAAAA GATCTGCCCCCATGGCCCAGGATATGCCACTGACGGAAGAGATATTGACGAATGTAAGGTGATGCCAAGC CTCTGTACTAATGGCCTGTGTGTCAACACCATGGGCTCCTTCCGGTGCTTCTGCAAGGTTGGCTACACCA CAGACATCAGCGGGACAGCTTGTGTAGACCTGGATGAATGTTCCCAGTCTCCAAAGCCATGCAACTTCAT CTGCAAGAACACCGAGGGCAGTTACCAGTGCTCCTGCCCACGGGGTTACGTCCTGCAGGAAGACGGCAAG ACGTGCAAAGACCTCGATGAATGTCAAACCAAACAGCACAACTGCCAGTTCCTCTGTGTCAACACCCTGG GGGGGTTCACCTGTAAATGTCCACCCGGTTTCACCCAGCATCACACGGCTTGCATTGATAACAATGAGTG TGGGTCCCAGCCTTCCCTCTGTGGAGCAAAGGGGATCTGTCAGAACACTCCTGGGAGCTTCAGCTGCGAA TGCCAAAGAGGCTTCTCCCTGGATGCCTCTGGGCTGAACTGCGAAGATGTTGACGAATGTGACGGAAACC ACAGGTGCCAACATGGCTGCCAGAACATCCTGGGGGGTTACCGGTGCGGCTGCCCGCAGGGATATGTCCA GCACTACCAGTGGAACCAGTGTGTTGATGAGAATGAATGTTCCAACCCCGGTGCCTGTGGCTCGGCCTCT TGCTATAACACCCTGGGGAGTTACAAGTGCGCCTGCCCCTCGCGGTTCTCCTTCGACCAGTTCTCCAGTG CCTGTCACGACGTGAACGAGTGCTCCTCCTCCAAGAACCCGTGCAGCTACGGCTGCTCCAACACGGAAGG AGGCTACCTCTGTGGCTGCCCACCAGGCTACTTCAGAGTGGGACAGGGCCACTGTGTCTCGGGAATGGGC TTCAACAAAGGACAGTACCTGTCAGTGGATTCGGAGGCAGAGGACGACGAAAACGCTCTGTCCCCAGAAG CATGCTACGAGTGCAAAATCAACGGCTACGCCAAGAAAGATGGCCGGCGGAAGAGGAGCGCGTCAGAGCC TGAGCCCGCCTCAGCAGAGGAGCAAATCAGCCTAGAAAGCGTTGCCATGGACAGCCCAGTCAACATGAAG TTCAACCTCTCCGGCCTCGGCTCCAAGGAGCACATCCTAGAACTGGTCCCCGCCATTGAGCCCCTCAACA ACCACATCCGTTACGTCATCTCCCAAGGGAATGACGATGGTGTCTTCCGAATCCATCAAAGGAATGGGCT CAGCTACCTGCACACAGCCAAGAAGAAGCTCACTCCCGGCACATACACACTGGAAATCACCAGCATCCCT CTTTATGGGAAGAAGGAGCTAAGGAAATTGGAGGAACGCAATGAGGGCAGCTACCTCCTGGGGGTGCTGG GGGAGGCTCTCAGAATGAGGTTGCAGATTCAGCTGTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031826
Insert Size	8721 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031826.1, NP_114014.1
RefSeq Size	8756 bp
RefSeq ORF	8721 bp
Locus ID	689008
Gene Summary	extracellular matrix protein which serves as a ligand for alphavbeta3 integrin [RGD, Apr 2007]

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