Gpx6 (NM_147165) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Synonyms	OBPII; Ry2d1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210731 representing NM_147165 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCAGCAGTTCTGGGGTCCCTGTCTTTTCTCATTGTTTATGGCTGTATTGGCTCAGGAGACACTGG ATCCTCAAAAATCAAAGGTGGATTGCAACAAAGGGGTGGCTGGCACCGTCTATGAGTACGGAGCCAACAC CTTAGATGGTGGGGAGTATGTCCAGTTCCAGCAGTATGCAGGAAAGCACATTCTCTTTGTCAACGTGGCG TCCTTCTGTGGCCTGACAGCTACGTACCCTGAACTGAACACATTGCAGGAGGAGCTGAGACCATTCAATG TCAGTGTTTTGGGCTTTCCATGCAACCAGTTTGGAAAACAAGAACCTGGAAAGAACTCAGAGATCCTCCT TGGACTCAAATATGTTCGCCCAGGCGGTGGCTTTGTTCCCAATTTCCAGCTCTTTGAGAAGGGGGATGTG AACGGAGACAATGAACAAAAGGTTTTTTCTTTCCTAAAGAGCTCCTGCCCTCCCACCTCTGAACTTCTTG GCTCTCCAGAACATCTCTTCTGGGATCCCATGAAGGTCCATGACATTCGCTGGAACTTTGAGAAGTTCCT GGTGGGACCGGATGGAGCCCCTGTCATGCGCTGGTTCCACCAGACTCCTGTCAGAGTTGTCCAGTCAGAC ATCATGGAGTACCTAAACCAAACCCGTACCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_147165
Insert Size	666 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_147165.1, NP_671694.1
RefSeq Size	1151 bp
RefSeq ORF	666 bp
Locus ID	259233
UniProt ID	Q64625
Gene Summary	This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidases catalyze the reduction of a variety of hydroperoxides using glutathione as a specific electron donor substrate, and thereby protect cells against oxidative damage. Expression of this gene is restricted to embryos and adult olfactory epithelium. The mouse and rat orthologs contain a cysteine (Cys) residue at the active site, unlike the human counterpart, which is a selenoprotein, containing selenocysteine (Sec) instead. [provided by RefSeq, Jul 2017]

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