Hck (NM_013185) Rat Untagged Clone
CAT#: RN210580
Hck (untagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug)
CNY 6,750.00
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Hctk; p56Hck; p59Hck |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN210580 representing NM_013185
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGGGGGTCGTTCGAGCTGCGAGGATCCGGGCTGCCCGCGAGGCGAAGGGCGGGTGCCCAGGATGGGAT GTGTGAAGTCCAGGTTCCTCCGAGAAGGAAGCAAGGCCTCAAAAATAGAGCCAAATGCCAACCAGAAAGG CCCTGTGTATGTGCCGGATCCCACGTCCCCTAAGAAGCTGGGACCGAACAGCATCAACAGCCTGCCCCCG GGGGTCGTGGAGGGCTCTGAGGACACCATTGTGGTCGCACTGTACGACTATGAGGCCATTCACCGTGAAG ACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGTCTGGGGAGTGGTGGAAGGCCCGTTC CCTGGCTACCAAGAAAGAAGGCTATATCCCAAGCAATTATGTAGCTCGAGTTAACTCTTTGGAGACTGAG GAGTGGTTCTTCAAGGGTATCAGCCGGAAGGATGCAGAGCGCCACCTGCTGGCTCCCGGGAACATGCTGG GCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCACTTTCTGTTCGAGACTTTGACCC CCAGCACGGAGACACGGTGAAGCATTATAAAATCCGGACACTGGACAGTGGAGGCTTCTACATCTCTCCG AGGAGCACCTTCAGCAGCCTGCAGGAACTTGTCGTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGA AGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCCTGGGAGATTCCTCG AGAATCCCTCCAGATGGAGAAGAAACTGGGAGCCGGGCAGTTTGGAGAAGTGTGGATGGCCACCTACAAC AAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCTGTGGAGGCCTTCCTGGCAGAGG CCAACCTGATGAAGACGTTACAGCATGATAAACTGGTGAAGCTGCACGCCGTGGTCTCTCAGGAGCCCAT CTTTATTGTCACCGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAGAAGGCAGCAAG CAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATTTCAGAGGGCATGGCTTTCATTGAGCAGAGGA ACTACATCCACCGAGACCTCCGGGCTGCCAACATCTTGGTTTCTGCATCACTGGTGTGTAAGATCGCTGA CTTTGGACTGGCACGGATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGTTCCCCATCAAG TGGACAGCTCCTGAAGCCATCAACTTTGGCTCCTTCACCATCAAGTCAGATGTCTGGTCCTTTGGTATCC TGCTGATGGAAATCGTCACCTACGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAGGTGATTCGAGC ACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGCCCAGAGGAGCTCTACAGTATCATGATCCGC TGCTGGAAGAACCGTCCAGAGGAACGGCCCACTTTCGAATACATCCAGAGCGTGCTGGATGACTTCTACA CGGCCACTGAGAGCCAGTATCAGCAGCAACCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_013185 |
Insert Size | 1575 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013185.3, NP_037317.2 |
RefSeq Size | 2081 bp |
RefSeq ORF | 1575 bp |
Locus ID | 25734 |
UniProt ID | P50545 |
Gene Summary | The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types.The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms in human and mouse. Based on strong sequence conservation amongst these species, this may also be true for rat. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR210580 | Hck (Myc-DDK-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug) |
CNY 6,750.00 |
|
RR210580L3 | Lenti ORF clone of Hck (Myc-DDK-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug) |
CNY 8,650.00 |
|
RR210580L4 | Lenti ORF clone of Hck (mGFP-tagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug) |
CNY 9,500.00 |