Hck (NM_013185) Rat Untagged Clone

CAT#: RN210580

Hck (untagged ORF) - Rat hemopoietic cell kinase (Hck), (10 ug)



  "NM_013185" in other vectors (3)

CNY 6,750.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Hctk; p56Hck; p59Hck
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210580 representing NM_013185
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

CTGGGGGGTCGTTCGAGCTGCGAGGATCCGGGCTGCCCGCGAGGCGAAGGGCGGGTGCCCAGGATGGGAT
GTGTGAAGTCCAGGTTCCTCCGAGAAGGAAGCAAGGCCTCAAAAATAGAGCCAAATGCCAACCAGAAAGG
CCCTGTGTATGTGCCGGATCCCACGTCCCCTAAGAAGCTGGGACCGAACAGCATCAACAGCCTGCCCCCG
GGGGTCGTGGAGGGCTCTGAGGACACCATTGTGGTCGCACTGTACGACTATGAGGCCATTCACCGTGAAG
ACCTCAGCTTCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGTCTGGGGAGTGGTGGAAGGCCCGTTC
CCTGGCTACCAAGAAAGAAGGCTATATCCCAAGCAATTATGTAGCTCGAGTTAACTCTTTGGAGACTGAG
GAGTGGTTCTTCAAGGGTATCAGCCGGAAGGATGCAGAGCGCCACCTGCTGGCTCCCGGGAACATGCTGG
GCTCCTTCATGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCACTTTCTGTTCGAGACTTTGACCC
CCAGCACGGAGACACGGTGAAGCATTATAAAATCCGGACACTGGACAGTGGAGGCTTCTACATCTCTCCG
AGGAGCACCTTCAGCAGCCTGCAGGAACTTGTCGTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGA
AGCTGTCAGTGCCCTGTGTGTCTCCCAAACCCCAGAAGCCATGGGAGAAAGATGCCTGGGAGATTCCTCG
AGAATCCCTCCAGATGGAGAAGAAACTGGGAGCCGGGCAGTTTGGAGAAGTGTGGATGGCCACCTACAAC
AAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCTGTGGAGGCCTTCCTGGCAGAGG
CCAACCTGATGAAGACGTTACAGCATGATAAACTGGTGAAGCTGCACGCCGTGGTCTCTCAGGAGCCCAT
CTTTATTGTCACCGAGTTCATGGCCAAAGGAAGCCTGCTGGACTTTCTCAAGAGTGAAGAAGGCAGCAAG
CAGCCACTGCCAAAACTCATTGACTTCTCAGCCCAGATTTCAGAGGGCATGGCTTTCATTGAGCAGAGGA
ACTACATCCACCGAGACCTCCGGGCTGCCAACATCTTGGTTTCTGCATCACTGGTGTGTAAGATCGCTGA
CTTTGGACTGGCACGGATCATCGAGGACAATGAGTACACAGCTCGGGAAGGAGCCAAGTTCCCCATCAAG
TGGACAGCTCCTGAAGCCATCAACTTTGGCTCCTTCACCATCAAGTCAGATGTCTGGTCCTTTGGTATCC
TGCTGATGGAAATCGTCACCTACGGCCGGATCCCTTACCCAGGTATGTCAAACCCAGAGGTGATTCGAGC
ACTAGAGCATGGGTACCGTATGCCTCGACCAGATAACTGCCCAGAGGAGCTCTACAGTATCATGATCCGC
TGCTGGAAGAACCGTCCAGAGGAACGGCCCACTTTCGAATACATCCAGAGCGTGCTGGATGACTTCTACA
CGGCCACTGAGAGCCAGTATCAGCAGCAACCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_013185
Insert Size 1575 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013185.3, NP_037317.2
RefSeq Size 2081 bp
RefSeq ORF 1575 bp
Locus ID 25734
UniProt ID P50545
Gene Summary The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types.The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms in human and mouse. Based on strong sequence conservation amongst these species, this may also be true for rat. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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