Col16a1 (NM_001015033) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MGC109452
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209804 representing NM_001015033 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTATCCTGGGCTCCCGGCCTATGGGTGCTTGGGCTCTGGGCCACCTTCAGCCATGGAACAAATA TAGGTGAGCGCTGCCCAACTTCACAGGAAGAAGGACTCAAGCTGGAACACAGCAGTGACCCGTCAACCAA CATGACTGGCTTCAACCTAATCCGCAGGCTGAACCTTATGAAGACATCCGCCATCAAAAAGATCCGCAAC CCCAAGGGGCCTCTCATCTTGCGACTGGGGGCTGCCCCACTGACCCAAGCAACTCGACGAGTGTTCCCTC GGGGCCTCCCCGAGGAGTTTGCCCTGGTCCTGACGGTCCTTCTGAAGAAACACACCTTCCGGAATACATG GTACTTATTCCAAGTGACTGACGCAAATGGGTACCCACAGGTCTCCTTGGAAGTCAACAGCCAGGAGCGG ACTCTAGAGCTCCGGGCACAGGGCCAAGATGGTGACTTTGTGTCCTGCATCTTCCCAGTGCCTCAGCTCT TCGATCTGCGTTGGCACAAGCTGATGCTGAGCGTGGCAAGCCGTGTGGCCTCTGTGCATGTGGACTGCGT CTCGGCTTCCTCCCAGCCTCTGGGGCCCCGGCAGCCCATCCGGCCTGGGGGACATGTGTTTCTGGGCTTG GACGCTGAGCAGGGCAAGCCGGTTTCATTTGACCTTCAGCAGGCGCACATCTACTGTGACCCGGAGCTGG TGCTGGAGGAAGGCTGCTGTGAGATCTTACCGGGAGGGTGTCCCCCAGAGACCTCCAAGTCACGCCGGGA CACCCAGAGTAATGAGCTCATTGAGATCAATCCTCAGACCGAGGGCAAGGTCTACACCCGCTGCTTCTGC TTGGAAGAACCTCATAACAGCAAGGTGGACGCACAGCTGATGGGAAGAAGCAGCCAGAAGGCAGAAAGGG GGACAAAGGCCCACCATGAGACTGGAGCCAGCGAGTGTCCGCCCTGTGCCCGCAGCGCCCGGGAGAGCAA TGTCACACTTGGTCCCCCTGGCCTCAAGGGAGGGAAAGGTGACCAAGGCCTGACTGGACCATCAGGCCCC AAGGGAGAGAAGGGAGCACGGGGCAATGACTGTGTTCGAATCTCCCCAGATGGCCCACTTCAGTGTGCAG AAGGTCCGAAAGGAGAGAAGGGAGAATCAGGAGACTTGGGACCCTCAGGACTCCCGGGCCCAACAGGCCA GAAGGGCCAGAAAGGGGAGAAGGGCGATGGAGGCCTCAAAGGACTGCCGGGAAAGCCGGGGCGAGATGGT CGGCCAGGAGAGATCTGTGTCATTGGGCCCAAAGGGCAGAAAGGAGACCCTGGCTTTGTTGGGCCTGAGG GATTGGCAGGAGAGCCTGGGCCCCCTGGCCTACCCGGACCACCTGGGATAGGACTGCCTGGGACTCCTGG AGATCCAGGTGGCCCACCAGGCCCTAAAGGAGCAAAGGGCAGCTCTGGAATACCAGGAAAGGAAGGCCCA GGAGGGAAGCCGGGGAAGCCAGGAGTACCCGGGACTAAGGGAGAAAAGGGTGACCCCTGTGAAGTGTGTC CAACGCTGCCCGAAGGGTTCCAGAACTTTGTGGGGCTCCCTGGAAAGCCAGGGCCTAAAGGAGAACCAGG TGATCCTGCTCCAGCCAGGGGAGATCCAGGCATCCAAGGAATGAAAGGAGAGAAGGGGGAACCCTGCTCA TCCTGCAGTTCAGGTGTTGGGGCCCAGCATCTTGGGCCCTCCACTGGGGTCAATGAAGACATGGGCTCCC CTGTCCATGGTTTACCTGGCCTTCCGGGCAAACCTGGAATTCCCGGGCCAAGAGGCATGAAAGGAGAGAA GGGTAGTTTTGGCGATGCTGGGCCGGCTGGAGTTGCGGGACCACCAGGACCAGTGGGACCGGCTGGTATC AAAGGGGCAAAGGGGGAGCCGTGTGAGCCCTGTGCATCTTTGTCCAAGCTCCAAGATGGGGACACTCAAG TGGTTCACCTGCCCGGCCCAGCTGGAGAGAAGGGAGAGCCTGGGCCCCCGGGCTCCGGGCTGCCTGGGAA GCAGGGTAGAGCTGGAGAGCGTGGACTCAAGGGGCAGAAGGGGGATGCTGGGAATCCAGGAGACCCTGGA ACGCCAGGCATCACAGGGCAGCCTGGAATGTCTGGAGAGCCTGGGGTTCGGGGTCCTGCAGGACCAAAAG GAGAAAAGGGCGATGGATGCACTGCCTGCCCTAGCCTGCAGGGGGCGTTGACAGATGTATCGGGACTGCC TGGGAAACCAGGTCCCAAAGGAGAGCCAGGTCCTGAAGGTGTGGGTCGGCCCGGTAAACCAGGCCAGCCT GGTCTACCAGGAGTTCAAGGGCCTCCAGGACTGAAGGGCACTCAGGGAGAGCCTGGACCTCCAGGCACCG GTGCTGAGGGACCCCAGGGGGAGCCTGGAACCCCAGGTTTGCCTGGCATTCAGGGGCCCCCCGGACCTCG GGGACCACCTGGCTCAACCGGCGAACACGGTGCCCAGGGACCTCCAGGGCCCAAAGGAGCCACAGGACCC ATGGGACCTCCTGGTGCTAGTATCTCTGGGCCTCCGGGCCATGAGGGGCTGAGAGGACAGACTGGACTTC AGGAGCATCACCGAGTACAGTGCCCGGGTAGCAGGGGCCAAAAGGGATCACGAGGAGAGAAAGGTGAACC GGGAGAGTGTTCCTGTCCTTCTCGAGGAGAGCCCATCTTCTCCGGCATGCCGGGTGCTCCGGGACTGTGG ATGGGCAGCTCCTCGCAGCCGGGCCCGCAGGGTCCCCCAGGTGTCCCCGGACCACCAGGTCCCCCCGGAA TGCCTGGATTGCAGGGAGTGCCTGGACACAACGGTTTGCCAGGACAGCCTGGGCTCACTGCCGAGCTGGG ATCCTTACCCATCGAGCAGCATCTCCTCAAGAGCATCTGTGGTGACTGTGCCCAGGGCCAGGCAGCCCAT TCAGCCTCCCTTCTGGTGAAAGGAGAAAAGGGGGACCAGGGTGTCCCTGGTGTGCCGGGTTTCAACGACT GTGCCAGGTGCTTCATAGAGAGGGAGCGCCCGAGAGCTGAGGAGGCCAGGGGAGACAACAGTGAGGGAGA ACCTGGCTGTCCTGGGAGCCCTGGCCTGCCTGGTCCTCCAGGGATGCCTGGCCAAAGAGGAGAAGAGGGT CCACCTGGCATGAGGGGCTCTCCTGGTCTTCCAGGCCCTGTTGGCCCCCCAGGTTTTCCTGGTGCTGTTG GCTCCCCCGGATTGCCTGGTCTTCAAGGGGAGCGAGGCCCCACAGGCCTGACTGGAGACAAGGGAGAACC GGGTCCCCCAGGACAGCCTGGCTACCCGGGTGCCATGGGTCCCCCAGGACTGCCTGGCATCAAGGGAGAA AGAGGATATGCTGGCCCTGCGGGAGAGAAGGGGGAGTCGGGCCCACCAGGATCTGAAGGCCTTCCAGGTC CCCAAGGTCCAGCGGGTCCCCGAGGAGAGCGAGGTCCCCAAGGGAGCTCGGGAGAGAAGGGTGATCAGGG ATTTCAAGGCCAGCCAGGTTTCCCTGGCCCACCGGGTCCCCCGGGATTCCCAGGCAAAGCTGGAGCTCCT GGCCCACCTGGCCCCCAAGCAGAGAAGGGCAGTGAAGGGATGCGAGGTCCCTCAGGCTTGCCTGGTTCCC CTGGACCACCAGGCCCTCCTGGGATTCAGGGCCCTGCTGGACTGGATGGACTGGATGGGAAAGACGGCAA ACCGGGCCTGAGGGGGGACCCAGGTCCCGCTGGCCCTCCTGGACTCATGGGACCTCCAGGTTTCAAAGGG AAAACAGGGCATCCTGGTCTCCCTGGACCTAAGGGTGACTGTGGAAAACCAGGTCCTCCTGGCAGCAGTG GCCGGCCAGGTGCAGAGGGTGAGCCCGGTGCCATGGGACCCCAGGGACGACCAGGACCTCCAGGGCATCT TGGGCCACAGGGGCCTCCAGGCCAGCCAGGTCCACCTGGGCTCTCCACCATGGGCCTGAAAGGAGATCGG GGATCCCCTGGAGAAAGGGGCTTGGCCGGCCTCCCAGGCCAGCCTGGCACACCCGGACACCCCGGCCCTC CGGGTGAACCTGGTTCGGATGGTGCAGCTGGCAAAGAGGGACCTCCAGGAAAGCAGGGACTCTATGGACC CCCCGGCCCAAAGGGTGACCCAGGACCTGCAGGACAGAAAGGCCAAGCAGGAGAAAAGGGAAGATCTGGC ATGCCTGGGGGCCCTGGCAAGAGTGGCTCCATGGGACCGATTGGACCACCGGGTCCTGCGGGAGAGAGAG GCCACCCTGGGTCCCCAGGGCCTGCTGGGAACCCTGGATTGCCTGGTTTGCCAGGCTCTATGGGAGACAT GGTGAATTATGATGATATCAAGAGGTTTATCAGACAAGAGATCATTAAACTGTTTGATGAGAGGATGGCT TACTACACCTCTAGGATGCAGTTCCCCATGGAGGTGGCAGCAGCTCCAGGCCGGCCAGGGCCTCCAGGGA AGGATGGCGCCCCAGGGCGGCCAGGTGCTCCAGGGTCACCTGGGCTCCCTGGTCAGATAGGCCGAGAAGG ACGCCAGGGTTTGCCAGGAATGAGAGGATTGCCTGGTACCAAAGGTGATAAGGGAGACATTGGTGTTGGC ATTGCAGGAGAAAATGGTCTCCCTGGCCCTCCAGGTCCTCAAGGGCCTCCAGGTTATGGCAAGATGGGTG CAACCGGGCCAATGGGCCAGCAAGGCATTCCTGGGATCCCTGGCCCCCCGGGGCCCATGGGCCAGCCAGG GAAGGCAGGCCACTGCAACCCCTCAGACTGTTTTGGGGCCATGCCAATGGAGCAGCAGTACCCGCCCATA AAAAGCATGAAGGGGCCTTTTGGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001015033
Insert Size	4857 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001015033.2, NP_001015033.2
RefSeq Size	5241 bp
RefSeq ORF	4857 bp
Locus ID	366474

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