Plxna3 (NM_001107581) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Plxn4; SEX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209762 representing NM_001107581 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACACTGTCTGCCTTCTCCCATTGCTATTCTTCACCATAGGAGGGTGCCTGGGTAGCAGCAGGCCGT TTCGTACCTTTGTGGTGACAGATACCACACTGACTCACCTGGCTGTGCACCGAGTGACTGGGGAGGTGTT TGTAGGTGCCGTGAACCGAGTTTTCAAGCTGGCTTCAAATCTAACTGAGCTACGGGCCCATGTCACAGGG CCCATTGAGGACAATGCTCGCTGCTACCCACCCCCTAGCATGCGTGTGTGCTCCCACCGCCTGGTGCCTG TGGACAATGTGAACAAGCTGCTCCTCATAGACTATGCAGCCCGTCGCTTGGTAGCTTGTGGCAGCATCTG GCAGGGCATTTGCCAGTTCCTGCGTCTGGATGACCTCTTCAAGTTGGGTGAGCCCCACCATCGCAAGGAA CACTATCTGTCAGGGGCCCAGGAGCCTGATTCCATGGCTGGCGTCATTGTTGAACAGGGCCAGGGGCCTA GCAAGCTGTTTGTGGGCACCGCTGTTGATGGCAAGTCTGAGTACTTTCCCACCTTGAGTTCCCGTAAGCT CATCGACGATGAGGACAGTGGAGATATGTTCAGTCTGGTGTACCAGGATGAGTTTGTCTCTTCTCAGATC AAGATCCCTTCAGACACACTGTCCTTGTACCCTGCCTTTGACATCTACTACATATATGGCTTTGTCAGTG CCTCCTTCGTGTACTTCTTAACATTGCAGCTGGATACCCAGCAGACACTGCTGGATACAGCAGGCGAGAA ATTCTTCACATCTAAGATTGTGCGCATGTGTGCCGGGGACTCAGAGTTCTACTCTTACGTAGAGTTCCCT ATTGGCTGCTCCTGGCGTGGTGTGGAGTACCGCTTGGTGCAGAGTGCCCATCTGGCCAAGCCAGGCCTGC TACTGGCCCAGGCTCTGGGTGTGCCAGCCGACGAGGATGTGCTCTTCACCATCTTCTCTCAAGGCCAGAA GAATCGAGCCAACCCACCTCGGCAGACCATCCTTTGCCTCTTCACCCTCAGCAGCATTAATGCCCACATT CGGCGCCGAATCCAATCGTGCTACCGTGGGGAGGGCACGCTGGCCTTGCCCTGGCTGCTGAATAAGGAGC TGCCCTGCATCAACACCCCCATGCAGATCAATGGAAACTTCTGTGGGTTAGTGTTGAATCAACCATTGGG TGGCCTACACGTGATTGAGGGGCTACCCTTGCTGGCTGACAGCACTGATGGCATGGCCAGTGTGGCTGCC TACACCTACCACCAGCACTCTGTGGTTTTCATCGGTACACGCAGCGGTAATTTGAAGAAGGTGCGAGTTG ATGGCTCTCAGGATGCCCAACTATATGAAACGGTCTCTGTGGTGCAGGGCACCCCCATACTTCGAGACCT GCTCTTCAGCCCTGACCACCGACACATCTACCTCCTGAGTGAGAAGCAGGTGAGCCAACTCCCGGTGGAG ACCTGTGAGCAGTATCTGAGCTGTGCTGCATGCCTGGGCTCAGGGGACCCACACTGTGGTTGGTGTGTGC TACAGCACAGGTGCTGCCGTGAAGGGGCCTGTCCAGGTGCCTCTGCCCCACACGGCTTTGCAGAAGAGCT GAACAAATGTATACAAGTGCGGGTTCGGCCTAATAATGTATCAGTGACATCCTCTGGGGTGCAGCTGACA GTAGCCATGCGCAATGTGCCAGACCTCAGTTTGGGTGTGAGCTGTTCCTTTGAGGAAGTGACTGAAAGTG AGGCTATTCTGCTGCCCTCTGGAGAGCTACGATGCCCTTCACCATCTCTTCAGGAGCTCCAGACACTTAC CAGAGGGCATGGGGCAACTCATACTGTGCGGCTTCAGCTGCTCTCCATGGAGACTGGTGTGAGGTTTGCT GGGGTTGACTTTGTCTTCTACAACTGCAGTGCCCTCCAGTCGTGTATGTCCTGTGTTGGCAGCCCTTACC CCTGCCACTGGTGTAAGTACCGTCATGTGTGCACCAGCCACCCACACGAGTGTTCCTTCCAGGAGGGCAG GGTCCACAGCCCTGAGGGCTGCCCAGAGATCCTGCCTCGAGGGGACCTCTTGATTCCTGTGGGTGTCATG CAGCCTCTAACTCTGCGGGCTAAGAACCTGCCACAGCCTCAGTCAGGGCAGAAGAATTATGAATGTGTGG TCCGTGTACAGGGGCGGCAACATCGGGTACCTGCAGTGCGCTTTAATAGCAGCAGCGTGCAGTGCCAGAA TGCCTCGTACTTCTATGAAGGTGATGAGTTTGGTGACACTGAACTGGACTTCTCTGTGGTCTGGGATGGA GATTTCCCCATTGATAAGCCTCCTAGCTTCCGAGCCCTTCTTTACAAGTGCTGGGCTCAGCGGCCTAGCT GTGGCCTCTGCCTCAAGGCTGATCCCCGATTCAACTGTGGCTGGTGCATCTCGGAGCACAGGTGCCAGCT TCGGGTTCACTGCCCAGCTCCCAAAAGTAACTGGATGCACCCAAGCCAGAAGGGGGCCCGATGCAGCCAT CCCCGAATCACCCAGATTCACCCACTAACAGGACCCAAGGAGGGGGGCACCCGGGTCACCATTGTGGGTG AGAACCTGGGCCTCACTTCCCGTGAGGTTGGCCTTCGAGTAGCTGGTGTACGTTGTAACTCCATTCCCAC CGAATATGTCAGTGCTGAAAGGATCGTATGTGAGATGGAGGAATCGTTGGTGCCCAGCCCACCACCTGGG CCTGCTGAGCTCTGTGTAGGTGATTGTTCTGCTGACTTCCGCACACAGTCCCAGCAACTCTACAGCTTTG TGACCCCAACACTTGACCGTGTAAGTCCTACTCGGGGCCCAGCTTCTGGGGGAACTCGGCTCACCATCTC TGGAACTTCTCTGGATGCTGGTAGCAGGGTCACAGTTATTATAAGAGATGGAGAGTGCCAGTTTGTGAGG AGAGATGCTGAGGCAATCGTGTGTATCTCACCTATCTCCACTCTGGGCCCCAGTCAGGCCCCTATCATCC TTGCCATCGACCATGCCAACATCTCCAGCACTGGAGTCATCTATACCTACACTCAGGACCCTACTGTCAC ACACCTTGAGCCCACCTGGAGCATCATCAATGGAAGCACCTCCATCACTGTGAGTGGAACCCATCTGCTG ACAGTGCAAGAACCCCGTGTACGAGCCAAGTACCGTGGTATTGAGACCACTAATACATGCCAGGTGATCA ACGACACTGCAATGCTATGTAAGGCCCCTGGCATCTTCCTTGGGCATCCTCAGCCTCGGGCCCAAGGCGA GCACCCTGATGAGTTTGGCTTCTTGCTGGACCACGTGCAGGCAGCCCGCTCCCTCAACCGTTCTTCCTTC ACCTACTACCCTGATCCCAGCTTTGAACCACTTGGGCCCTCTGGTGTGCTAGATGTCAAACCTGGTTCGC ATGTTGTATTGAAGGGCAAGAACCTGATCCCTGCTGCAGCTGGCAGCTCCCGCCTCAACTACACAGTGTT GATTGGAGGACAACCATGTGCACTAACTGTCTCAGATACTCAACTTCTGTGTGATTCCCCTAGCCAGACA GGCCGGCAACCTGTTATGGTGCTGGTGGGTGGCCTGGAGTTCTGGTTGGGCACCCTGCACATCACTGCTG ATCGGGCACTGACCTTACCGGCTATGGTGGGGCTGGCAGCAGGAGGAGGGCTCTTGCTGCTGGCCATCAC TGTTGTACTGGTTGCCTACAAGCGAAAGACTCAGGATGCAGACCGCACACTTAAGCGACTTCAGCTACAG ATGGACAACCTGGAGTCTCGTGTGGCCTTGGAGTGCAAGGAAGCTTTTGCTGAGTTACAGACAGATATCA ACGAACTGACAAACCACATGGATGGTGTGCAGATCCCCTTCTTGGACTACCGGACCTATGCTGTGCGCGT ACTTTTCCCTGGCATTGAGGCTCACCCAGTGCTCAAGGAGCTGGATACTCCCCCCAATGTGGAGAAAGCC CTGCGCCTATTTGGACAGTTGCTGCACAGCCGTGCCTTCCTGCTCACCTTCATCCACACTTTGGAGGCCC AGAGCAGTTTCTCCATGCGTGACCGTGGTACTGTGGCCTCACTCACCATGGTGGCCCTGCAGAGTCGGCT TGACTATGCCACTGGGCTGCTCAAGCAACTGCTGGCTGACCTCATAGAGAAAAACCTTGAGAGCAAGAAC CACCCAAAGCTGCTATTGCGCAGGACAGAGTCAGTGGCTGAGAAGATGCTCACCAACTGGTTCACATTCC TGCTGCATAAGTTCCTGAAGGAGTGCGCCGGGGAGCCACTCTTCCTGCTGTACTGCGCCATCAAGCAGCA GATGGAGAAAGGTCCCATCGATGCCATAACAGGCGAGGCCCGCTACTCCTTAAGTGAGGACAAGCTCATC CGTCAGCAGATCGACTATAAGACCCTGACTCTGCATTGTGTGTGCCCGGAGAGTGAGGGCAGTGCCCAGG TCCCGGTGAAGGTTCTTAACTGTGATAGCATCACCCAGGCCAAAGACAAGCTGTTGGATACCGTGTACAA GGGTATTCCATACTCTCAGCGCCCCAAAGCTGAGGACATGGACTTGGAATGGCGCCAGGGCCGTATGGCC CGAATCATCCTCCAAGATGAGGATATCACTACAAAGATTGAGTGTGATTGGAAGAGGATCAACTCACTGG CTCACTACCAGGTGACTGATGGTTCCTTAGTAGCACTGGTGCCCAAACAAGTGTCTGCCTATAACATGGC CAACTCATTCACCTTCACCCGTTCACTTAGTCGCTATGAGAGTTTGCTCCGTGCTGCCAGCAGCCCAGAT AGCCTCCGTTCCCGAGCACCTATGCTCACACCTGACCAGGAGGCGGGCACCAAGCTGTGGCATCTGGTGA AGAACCACGACCATGCTGATCACCGAGAGGGAGACCGTGGCAGCAAGATGGTCTCAGAAATATATCTGAC AAGGCTGCTGGCCACCAAGGGCACGTTGCAGAAGTTTGTGGATGACTTGTTTGAAACTGTGTTCAGTACA GCCCACCGGGGCTCAGCCTTACCCTTGGCCATCAAGTACATGTTTGACTTCCTGGATGAACAGGCTGACC AGCGCCAGATCAGTGACCCTGATGTGCGCCACACCTGGAAAAGCAACTGCTTGCCTCTGCGTTTCTGGGT GAATGTGATCAAGAACCCACAATTCGTGTTTGACATCCACAAGAATAGCATCACAGATGCCTGCCTCTCT GTGGTAGCCCAGACCTTCATGGACTCCTGTTCCACATCAGAGCACCGCCTGGGCAAGGACTCACCTTCCA ACAAGCTGCTCTATGCCAAGGATATTCCCAATTACAAGAGCTGGGTGGAGAGGTACTACCGAGATATTGC AAAGATGGCATCCATCAGTGACCAGGACATGGACGCCTACTTAGTGGAGCAGTCCCGCCTCCATGCCAAT GACTTCAATGTCCTAAGTGCACTCAGTGAGCTCTACTTCTATGTCACCAAGTACCGTCAGGAGATCCTCA CCTCGCTGGACCGAGATGCCTCTTGTCGGAAGCATAAGCTTCGACAGAAGCTGGAGCAGATCATCACCTT GGTGTCCAGCAGCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107581
Insert Size	5619 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107581.2, NP_001101051.2
RefSeq Size	7376 bp
RefSeq ORF	5619 bp
Locus ID	309280
UniProt ID	D3ZPX4
Gene Summary	Coreceptor for SEMA3A and SEMA3F. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Regulates the migration of sympathetic neurons, but not of neural crest precursors. Required for normal dendrite spine morphology in pyramidal neurons. May play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm (By similarity).[UniProtKB/Swiss-Prot Function]

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