United States
Germany
Japan
United Kingdom
China
Cyp4x1 (NM_145675) Rat Untagged Clone
CAT＃: RN209639
Cyp4x1 (untagged ORF) - Rat cytochrome P450 4X1 (Cyp4x1), (10 ug)
CNY 6,560.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN209639 representing NM_145675
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCTCCTGGCTGGAGAATCGCTGGGCGCGGCCCTTACACCTGGCTTTGGTGTTCTGCCTGGCGC TGGTGCTGATGCAGGCCGTGAAGCTCTACCTGAGAAGACAGAGGCTGCTGCGCGACCTACGCCCCTTCCC GGGTCCTACCGCCCACTGGCTCCTGGGACACCAGAAGTTTCTTCAGGAGGATAATATGGAGAAGCTTGAC GAGATTGTCAAAGAGTACCCTTGCGCCTTCCCCTGCTGGGTAGGACCCTTCCAAGCATTTTTCTACATCT ATGACCCAGACTATGCAAAGATTTTTCTGAGCAGAACAGACCCAAAGACCCAGTACCTGCACCAGCTCAT GACTCCCTTTCTTGGAAGAGGACTCCTGAATTTAGATGGGCCCAGGTGGTTCCAACACCGCTGCCTCCTG ACTCCTGCGTTCCATCAGGACATCCTAAAACCATGTGTGGACATGATGGCCCACTCTGTGAACATGATGC TGGATAAATGGGAGAAGACGTGGACCACGCAGGAAACAACCATCGAGGTTTTTGAACACATTAACTTGAT GACCCTGGACATAATAATGAAATGTGCTTTTGGCCAGGAGACCAACTGCCAGATAAACGGCACCTATGAG TCTTATGTGAAGGCAACATTTGAACTTGGTGAAATCATATCTTCTCGCTTGTACAATTTCTGGCATCATC ATGACATAATTTTCAAACTCAGTCCTAAGGGCCACTGCTTCCAGGAGTTAGGCAAAGTGATACATCAATG CACAGAAAAGATTATCCAGGACAGAAAGAAAACCCTCAAGGATCAAGTAAATCAGGATGACACTCAAACA TCTCAAAATTTTCTGGATATTGTTCTTTCTGCCCAGGCTGGAGATGAAAAGGCCTTCTCAGATGCTGACC TTCGGTCTGAGGTGAACACCTTCATGTGGGCAGGACACGACGCCTCTGCAGCTAGCATCTCCTGGCTCCT TTACTGCCTGGCTCTGAACCCTGAGCATCAAGACAGATGCCGGACAGAGATCAGGAGCATTCTGGGAGAT GGGTCTTCCATCACCTGGGAACAGCTGGATGAGATACCTTACACTACAATGTGCATCAAGGAGACACTCC GCTTGATTCCTCCCATCCCATCCATCTCCAGGGAACTCAGCAAACCCCTTACCCTCCCAGATGGACACTC GCTGCCTGCAGGAATGACTGTGGTCCTTAGTATTTGGGGTCTCCACCACAATCCTGCTGTCTGGAAAGAC CCAAAGGTCTTTGACCCTTTGAGATTCACTAAGGAGAATTCTGAACAGAGACACCCCTGCGCCTTCCTAC CATTCTCCAGTGGTCCAAGGAACTGCATCGGGCAGCAGTTTGCCATGCTGGAGTTAAAGGTGGCCATTGC CTTGACTCTGCTCCGCTTCCGAGTAGCTGCAGACCTCACCAGGCCTCCTGCCTTCTCCAGCCACACTGTG CTCAGACCCAAGCATGGGATCTATTTGCACCTGAAGAAACTCCCTGAATGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145675 |
| Insert Size | 1524 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145675.1, NP_663708.1 |
| RefSeq Size | 1524 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 246767 |
| UniProt ID | Q8K4D6 |
| Gene Summary | A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR209639 | Cyp4x1 (Myc-DDK-tagged ORF) - Rat cytochrome P450 4X1 (Cyp4x1), (10 ug) |
CNY 6,560.00 |
|
| RR209639L3 | Lenti ORF clone of Cyp4x1 (Myc-DDK-tagged ORF) - Rat cytochrome P450 4X1 (Cyp4x1), (10 ug) |
CNY 8,460.00 |
|
| RR209639L4 | Lenti ORF clone of Cyp4x1 (mGFP-tagged ORF) - Rat cytochrome P450 4X1 (Cyp4x1), (10 ug) |
CNY 9,220.00 |
