Adcy5 (NM_022600) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209467 representing NM_022600 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGCTCCAAAAGCGTGAGCCCCCCGGGCTACGCTGCACAGACAGCAGCGTCGCCCGCGCCCCGGG GAGGCCCGGAGCATCGCGCCGCGTGGGGAGAAGCCGATTCCCGCGCCAATGGCTACCCCCACGCCCCCGG GGGATCGACCCGCGGCTCCACCAAGCGATCTGGGGGAGCGGTGACCCCACAACAGCAGCAGCGCCTGGCT AGCCGTTGGCGCGGTGGCGACGACGATGAAGACCCTCCACTAAGCGGTGACGACCCTCTGGTCGGAGGCT TCGGCTTCAGCTTCCGTTCTAAGTCGGCCTGGCAGGAGCGCGGCGGCGACGACGGCGGTCGCGGCAGCAG GCGGCAGCGGCGGGGCGCGGCTGGAGGGGGCAGCACCCGGGCGCCCCCTGCGGGCGGCAGTGGCAGTTCG GCGGCGGCCGCAGCAGCCGCAGGTGGCACGGAGGTGCGCCCCCGCTCGGTGGAGGTGGGCCTGGAGGAGC GTCGAGGAAAAGGCCGAGCGGCCGAGGAGCTGGAGCCCGGAACTGGCACCGTCGAGGATGGAGACGGGTC CGAGGATGGAGGCAGTTCCGTGGCGTCAGGCTCTGGGACCGGCACGGTGCTGTCGTTGGGCGCCTGCTGC CTGGCCTTGCTGCAGATATTCCGCTCTAAGAAGTTCCCGTCGGACAAACTGGAGCGTCTGTACCAGCGCT ATTTCTTCCGCCTGAACCAGAGCAGCCTCACCATGCTCATGGCCGTGCTGGTGCTCGTGTGCCTGGTCAT GCTCGCTTTCCACGCGGCACGGCCCCCGCTCCAGGTAGTCTACCTGGCCGTGTTGGCAGCTGCTGTGGGC GTGATCCTTATCATGGCTGTGCTCTGCAACCGTGCAGCCTTCCACCAGGACCACATGGGCCTGGCCTGCT ATGCGCTCATTGCAGTGGTGCTGGCCGTCCAGGTAGTGGGCCTGTTGCTGCCACAGCCACGCAGCGCCTC CGAGGGCATCTGGTGGACCGTGTTCTTCATCTATACCATCTACACCCTGCTGCCTGTGCGCATGAGGGCT GCGGTGCTCAGCGGGGTGCTTCTGTCGGCTCTCCACTTGGCCATCTCTCTGCACACCAACGCCCAGGACC AGTTTCTGCTGAAACAGCTTGTCTCCAACGTCCTCATCTTCTCTTGCACCAACATCGTGGGTGTGTGCAC TCACTACCCAGCCGAGGTCTCCCAGAGACAAGCCTTCCAGGAGACCCGGGAGTGCATCCAAGCTCGGCTC CACTCACAGCGGGAGAACCAGCAACAGGAGCGTCTCCTGCTGTCTGTCCTTCCCCGTCATGTTGCCATGG AGATGAAAGCAGACATCAACGCCAAACAGGAGGATATGATGTTCCACAAGATTTACATCCAGAAACATGA CAATGTGAGCATCCTGTTTGCTGACATCGAAGGCTTCACTAGCCTGGCATCCCAGTGTACTGCCCAAGAA CTGGTCATGACCCTCAACGAGCTCTTCGCCCGCTTTGACAAGTTGGCTGCGGAGAATCACTGCTTACGGA TTAAGATCCTCGGGGATTGTTACTACTGTGTCTCGGGGCTGCCTGAAGCCAGAGCTGACCACGCCCACTG CTGCGTAGAGATGGGAATGGACATGATCGAGGCCATCTCGTCGGTCCGGGAGGTGACAGGGGTGAACGTG AACATGCGTGTGGGAATTCACAGCGGGAGAGTACACTGCGGTGTCCTTGGCCTCAGAAAGTGGCAATTCG ACGTGTGGTCTAACGATGTCACACTGGCCAACCACATGGAAGCTGGCGGCAAGGCAGGCCGCATCCACAT CACCAAGGCCACACTCAACTACCTGAACGGGGACTATGAGGTGGAGCCAGGCTGTGGTGGTGAGCGCAAT GCCTACCTCAAGGAGCACAGCATCGAGACCTTCCTCATCCTGCGCTGTACCCAGAAGCGGAAAGAAGAGA AGGCCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATTGGACACAATCCGCCTCACTGGGGAGC TGAGCGCCCCTTCTACAACCACTTGGGTGGCAACCAGGTGTCCAAGGAAATGAAGAGGATGGGCTTTGAG GACCCCAAGGACAAGAATGCCCAGGAAAGTGCCAACCCTGAGGATGAAGTGGACGAGTTTCTGGGTCGAG CCATCGATGCCAGGAGTATTGACAGACTGCGATCCGAACACGTCCGAAAGTTCCTCTTGACCTTTAGGGA GCCCGACTTAGAGAAGAAGTACTCCAAGCAGGTGGATGACCGATTTGGTGCCTATGTGGCCTGTGCCTCG CTTGTTTTCCTCTTCATCTGCTTTGTCCAGATCACCATTGTGCCCCACTCCCTGTTCATGCTGAGCTTCT ACCTGTCGTGTTTCCTGCTACTGGCCTTGGTGGTGTTTATATCTGTGATCTACGCCTGTGTGAAGCTCTT CCCTACTCCCCTGCAGACACTCTCCAGGAAGATAGTGCGATCCAAGAAGAACAGCACCCTGGTCGGGGTG TTCACCATCACCCTGGTGTTCCTCTCGGCTTTTGTCAACATGTTCATGTGCAACTCTAAGAACCTGGTGG GTTGCCTGGCAGAGGAGCACAACATCACGGTGAACCAGGTGAACGCATGTCATGTGATGGAGTCGGCCTT CAACTACAGCCTGGGCGACGAGCAGGGCTTCTGTGGCAGCCCCCAGTCCAACTGCAACTTCCCAGAGTAC TTCACCTACAGCGTGCTGCTCAGCCTGCTGGCCTGCTCCGTGTTCCTGCAGATCAGCTGCATCGGGAAGC TGGTGCTCATGCTGGCCATCGAGCTCATCTACGTGCTCATCGTAGAGGTGCCCGGTGTCACACTCTTTGA CAACGCTGACCTTCTGGTCACCGCCAATGCCATAGACTTCAGCAACAACGGGACCTCCCAGTGCCCTGAG CATGCGACCAAGGTGGCGCTGAAGGTGGTGACGCCCATCATCATCTCTGTCTTCGTGCTGGCTCTGTATC TGCATGCTCAGCAGGTGGAATCCACTGCTCGCCTTGACTTCCTCTGGAAACTGCAGGCCACAGAAGAGAA GGAGGAGATGGAGGAGCTGCAGGCCTACAACCGGCGCTTGCTGCACAACATTCTGCCCAAGGACGTGGCT GCCCACTTCCTGGCCCGGGAGCGGCGCAATGATGAACTGTACTACCAATCCTGCGAGTGCGTGGCTGTCA TGTTTGCCTCCATCGCCAACTTCTCCGAATTCTACGTGGAGCTAGAGGCCAACAATGAGGGCGTTGAATG CCTACGGCTGCTCAATGAGATCATCGCAGACTTTGATGAGATCATCAGTGAGGATCGGTTCAGGCAGCTG GAAAAGATCAAGACCATAGGTAGCACCTACATGGCTGCCTCTGGCCTCAACGACTCCACCTATGACAAGG CAGGCAAGACCCACATCAAGGCTCTTGCAGACTTCGCCATGAAGCTGATGGACCAAATGAAGTACATCAA TGAGCACTCCTTCAACAACTTCCAGATGAAGATCGGGCTTAACATTGGACCTGTAGTGGCTGGGGTCATT GGGGCTCGCAAGCCTCAGTATGACATCTGGGGCAATACAGTAAATGTGGCCAGCCGTATGGACAGCACTG GGGTGCCTGACCGCATCCAGGTTACTACAGATATGTACCAGGTGCTGGCCGCCAACACATACCAGCTGGA GTGCCGGGGTGTGGTCAAGGTCAAGGGCAAGGGTGAGATGATGACCTACTTCCTCAATGGAGGGCCTCCC CTCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022600
Insert Size	3789 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022600.1, NP_072122.1
RefSeq Size	4847 bp
RefSeq ORF	3789 bp
Locus ID	64532
UniProt ID	Q04400
Gene Summary	enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate [RGD, Feb 2006]

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