Adcy5 (NM_022600) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209467 representing NM_022600 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGCTCCAAAAGCGTGAGCCCCCCGGGCTACGCTGCACAGACAGCAGCGTCGCCCGCGCCCCGGG GAGGCCCGGAGCATCGCGCCGCGTGGGGAGAAGCCGATTCCCGCGCCAATGGCTACCCCCACGCCCCCGG GGGATCGACCCGCGGCTCCACCAAGCGATCTGGGGGAGCGGTGACCCCACAACAGCAGCAGCGCCTGGCT AGCCGTTGGCGCGGTGGCGACGACGATGAAGACCCTCCACTAAGCGGTGACGACCCTCTGGTCGGAGGCT TCGGCTTCAGCTTCCGTTCTAAGTCGGCCTGGCAGGAGCGCGGCGGCGACGACGGCGGTCGCGGCAGCAG GCGGCAGCGGCGGGGCGCGGCTGGAGGGGGCAGCACCCGGGCGCCCCCTGCGGGCGGCAGTGGCAGTTCG GCGGCGGCCGCAGCAGCCGCAGGTGGCACGGAGGTGCGCCCCCGCTCGGTGGAGGTGGGCCTGGAGGAGC GTCGAGGAAAAGGCCGAGCGGCCGAGGAGCTGGAGCCCGGAACTGGCACCGTCGAGGATGGAGACGGGTC CGAGGATGGAGGCAGTTCCGTGGCGTCAGGCTCTGGGACCGGCACGGTGCTGTCGTTGGGCGCCTGCTGC CTGGCCTTGCTGCAGATATTCCGCTCTAAGAAGTTCCCGTCGGACAAACTGGAGCGTCTGTACCAGCGCT ATTTCTTCCGCCTGAACCAGAGCAGCCTCACCATGCTCATGGCCGTGCTGGTGCTCGTGTGCCTGGTCAT GCTCGCTTTCCACGCGGCACGGCCCCCGCTCCAGGTAGTCTACCTGGCCGTGTTGGCAGCTGCTGTGGGC GTGATCCTTATCATGGCTGTGCTCTGCAACCGTGCAGCCTTCCACCAGGACCACATGGGCCTGGCCTGCT ATGCGCTCATTGCAGTGGTGCTGGCCGTCCAGGTAGTGGGCCTGTTGCTGCCACAGCCACGCAGCGCCTC CGAGGGCATCTGGTGGACCGTGTTCTTCATCTATACCATCTACACCCTGCTGCCTGTGCGCATGAGGGCT GCGGTGCTCAGCGGGGTGCTTCTGTCGGCTCTCCACTTGGCCATCTCTCTGCACACCAACGCCCAGGACC AGTTTCTGCTGAAACAGCTTGTCTCCAACGTCCTCATCTTCTCTTGCACCAACATCGTGGGTGTGTGCAC TCACTACCCAGCCGAGGTCTCCCAGAGACAAGCCTTCCAGGAGACCCGGGAGTGCATCCAAGCTCGGCTC CACTCACAGCGGGAGAACCAGCAACAGGAGCGTCTCCTGCTGTCTGTCCTTCCCCGTCATGTTGCCATGG AGATGAAAGCAGACATCAACGCCAAACAGGAGGATATGATGTTCCACAAGATTTACATCCAGAAACATGA CAATGTGAGCATCCTGTTTGCTGACATCGAAGGCTTCACTAGCCTGGCATCCCAGTGTACTGCCCAAGAA CTGGTCATGACCCTCAACGAGCTCTTCGCCCGCTTTGACAAGTTGGCTGCGGAGAATCACTGCTTACGGA TTAAGATCCTCGGGGATTGTTACTACTGTGTCTCGGGGCTGCCTGAAGCCAGAGCTGACCACGCCCACTG CTGCGTAGAGATGGGAATGGACATGATCGAGGCCATCTCGTCGGTCCGGGAGGTGACAGGGGTGAACGTG AACATGCGTGTGGGAATTCACAGCGGGAGAGTACACTGCGGTGTCCTTGGCCTCAGAAAGTGGCAATTCG ACGTGTGGTCTAACGATGTCACACTGGCCAACCACATGGAAGCTGGCGGCAAGGCAGGCCGCATCCACAT CACCAAGGCCACACTCAACTACCTGAACGGGGACTATGAGGTGGAGCCAGGCTGTGGTGGTGAGCGCAAT GCCTACCTCAAGGAGCACAGCATCGAGACCTTCCTCATCCTGCGCTGTACCCAGAAGCGGAAAGAAGAGA AGGCCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATTGGACACAATCCGCCTCACTGGGGAGC TGAGCGCCCCTTCTACAACCACTTGGGTGGCAACCAGGTGTCCAAGGAAATGAAGAGGATGGGCTTTGAG GACCCCAAGGACAAGAATGCCCAGGAAAGTGCCAACCCTGAGGATGAAGTGGACGAGTTTCTGGGTCGAG CCATCGATGCCAGGAGTATTGACAGACTGCGATCCGAACACGTCCGAAAGTTCCTCTTGACCTTTAGGGA GCCCGACTTAGAGAAGAAGTACTCCAAGCAGGTGGATGACCGATTTGGTGCCTATGTGGCCTGTGCCTCG CTTGTTTTCCTCTTCATCTGCTTTGTCCAGATCACCATTGTGCCCCACTCCCTGTTCATGCTGAGCTTCT ACCTGTCGTGTTTCCTGCTACTGGCCTTGGTGGTGTTTATATCTGTGATCTACGCCTGTGTGAAGCTCTT CCCTACTCCCCTGCAGACACTCTCCAGGAAGATAGTGCGATCCAAGAAGAACAGCACCCTGGTCGGGGTG TTCACCATCACCCTGGTGTTCCTCTCGGCTTTTGTCAACATGTTCATGTGCAACTCTAAGAACCTGGTGG GTTGCCTGGCAGAGGAGCACAACATCACGGTGAACCAGGTGAACGCATGTCATGTGATGGAGTCGGCCTT CAACTACAGCCTGGGCGACGAGCAGGGCTTCTGTGGCAGCCCCCAGTCCAACTGCAACTTCCCAGAGTAC TTCACCTACAGCGTGCTGCTCAGCCTGCTGGCCTGCTCCGTGTTCCTGCAGATCAGCTGCATCGGGAAGC TGGTGCTCATGCTGGCCATCGAGCTCATCTACGTGCTCATCGTAGAGGTGCCCGGTGTCACACTCTTTGA CAACGCTGACCTTCTGGTCACCGCCAATGCCATAGACTTCAGCAACAACGGGACCTCCCAGTGCCCTGAG CATGCGACCAAGGTGGCGCTGAAGGTGGTGACGCCCATCATCATCTCTGTCTTCGTGCTGGCTCTGTATC TGCATGCTCAGCAGGTGGAATCCACTGCTCGCCTTGACTTCCTCTGGAAACTGCAGGCCACAGAAGAGAA GGAGGAGATGGAGGAGCTGCAGGCCTACAACCGGCGCTTGCTGCACAACATTCTGCCCAAGGACGTGGCT GCCCACTTCCTGGCCCGGGAGCGGCGCAATGATGAACTGTACTACCAATCCTGCGAGTGCGTGGCTGTCA TGTTTGCCTCCATCGCCAACTTCTCCGAATTCTACGTGGAGCTAGAGGCCAACAATGAGGGCGTTGAATG CCTACGGCTGCTCAATGAGATCATCGCAGACTTTGATGAGATCATCAGTGAGGATCGGTTCAGGCAGCTG GAAAAGATCAAGACCATAGGTAGCACCTACATGGCTGCCTCTGGCCTCAACGACTCCACCTATGACAAGG CAGGCAAGACCCACATCAAGGCTCTTGCAGACTTCGCCATGAAGCTGATGGACCAAATGAAGTACATCAA TGAGCACTCCTTCAACAACTTCCAGATGAAGATCGGGCTTAACATTGGACCTGTAGTGGCTGGGGTCATT GGGGCTCGCAAGCCTCAGTATGACATCTGGGGCAATACAGTAAATGTGGCCAGCCGTATGGACAGCACTG GGGTGCCTGACCGCATCCAGGTTACTACAGATATGTACCAGGTGCTGGCCGCCAACACATACCAGCTGGA GTGCCGGGGTGTGGTCAAGGTCAAGGGCAAGGGTGAGATGATGACCTACTTCCTCAATGGAGGGCCTCCC CTCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209467 representing NM_022600 Red=Cloning site Green=Tags(s) MSGSKSVSPPGYAAQTAASPAPRGGPEHRAAWGEADSRANGYPHAPGGSTRGSTKRSGGAVTPQQQQRLA SRWRGGDDDEDPPLSGDDPLVGGFGFSFRSKSAWQERGGDDGGRGSRRQRRGAAGGGSTRAPPAGGSGSS AAAAAAAGGTEVRPRSVEVGLEERRGKGRAAEELEPGTGTVEDGDGSEDGGSSVASGSGTGTVLSLGACC LALLQIFRSKKFPSDKLERLYQRYFFRLNQSSLTMLMAVLVLVCLVMLAFHAARPPLQVVYLAVLAAAVG VILIMAVLCNRAAFHQDHMGLACYALIAVVLAVQVVGLLLPQPRSASEGIWWTVFFIYTIYTLLPVRMRA AVLSGVLLSALHLAISLHTNAQDQFLLKQLVSNVLIFSCTNIVGVCTHYPAEVSQRQAFQETRECIQARL HSQRENQQQERLLLSVLPRHVAMEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQE LVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISSVREVTGVNV NMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLNYLNGDYEVEPGCGGERN AYLKEHSIETFLILRCTQKRKEEKAMIAKMNRQRTNSIGHNPPHWGAERPFYNHLGGNQVSKEMKRMGFE DPKDKNAQESANPEDEVDEFLGRAIDARSIDRLRSEHVRKFLLTFREPDLEKKYSKQVDDRFGAYVACAS LVFLFICFVQITIVPHSLFMLSFYLSCFLLLALVVFISVIYACVKLFPTPLQTLSRKIVRSKKNSTLVGV FTITLVFLSAFVNMFMCNSKNLVGCLAEEHNITVNQVNACHVMESAFNYSLGDEQGFCGSPQSNCNFPEY FTYSVLLSLLACSVFLQISCIGKLVLMLAIELIYVLIVEVPGVTLFDNADLLVTANAIDFSNNGTSQCPE HATKVALKVVTPIIISVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVA AHFLARERRNDELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIISEDRFRQL EKIKTIGSTYMAASGLNDSTYDKAGKTHIKALADFAMKLMDQMKYINEHSFNNFQMKIGLNIGPVVAGVI GARKPQYDIWGNTVNVASRMDSTGVPDRIQVTTDMYQVLAANTYQLECRGVVKVKGKGEMMTYFLNGGPP LS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022600
ORF Size	3786 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022600.1, NP_072122.1
RefSeq Size	4847 bp
RefSeq ORF	3789 bp
Locus ID	64532
UniProt ID	Q04400
MW	139.2 kDa
Gene Summary	enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate [RGD, Feb 2006]

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