Pkdrej (NM_001134866) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209458 representing NM_001134866 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCCGGGCCCCGCGCTGCTGCTCCTGGGCCTGGGCCTGGGTCTGGGCAGCCAGCCGCCACCCCCCG GCCCTCGCGGGCTGCCGGGCGTGCTCAGGGGCGCCCCGGGGCTGGGGCAGGGCGCAGAGAGCAGCGTCCG GGGCGGTGGCACCGGTGGCCTGTCCCCGCGCTCAGTCCCGCGCCGCGCCAGCCCTACGCTGCCCCGTAGG TGTCCCTCGGGCGCAGCCGCGCGCGTCCTCCTGAAAGTCAATTCCTTCGACCCCGCAGCCGCCAAGGCTA CGGTGTCCTGCCAGACGGCCAACTGTATCATACAGCCAGTGAAGATTATCAACAGGAAGGACCAGAACGC TCCCCTCATCCTGAGCAGAGACCAGGAAGTCACCCTCAATGTCACTATACGTTGGCACTGTCCTGATGCC TTGGTCGTTCGCAAGACCTGGCTGTACTTTTCGGTGGCCTCTGTGAACGACACTCCCGACTGGAACCGAC CTGTGCAATTGCCACATGTCCAAGTGACTAAGGTTTCCTTCCTCCACATCCCCAAATATGCCCTGCCTTA CGGGGTGTATGTGTTTAACTTCACGCTGTCCCTCGTCAGGTGGGACCCCGTCTTGCCCACGGTGACAGAC TCGGATATCATCTACATTTGGGTTAAAAAACGCCCCCTGAAGGCTGTCCTTCCTGGAGCCTCCAGAGTAA CTGTGAGATTCTCGGATGCACTGATCCTCAATGGAAGCATGTCCTACGACCCAGAGGCAGACATTCCCAC AGAGGGACTCATCTTTTTTTGGTACTGTACCACAAATCCAAGATACTATAGTGACAACTACATGTTAGTG ACCAACCGGGATGTCTGTCACCCAAAGCAGGATTCACTGGACTGGCCTTGGGCAGTGGGCCCTGTACTAA CCATTCCAGCAGAGACTCTTAGGGGCAATGGTGTGTATTACTTCAGAATGGTGGTGCAGAAGCAAAATAG GACTGCATTTTCTGATAAAACTGTACACGTGCTCCAAGGACTTCAGCCCAAAGCCCACATTTCATGCATT GAAAACTGTGGCCACACTTTAGCCGTATCAGATAGATTCTCTCTGTTTCTGAACTGCTCAAGTTGTGGCA GTCGGGATTTCTACAGCTGGTCCATTTTGTCTCGGATGGGTAAGGAAGTGATGTTTGACTGGGCAGGACA AGCTGTCACCAGGAGGGATGGGGCTTACTTGTCCATGAAAGCTTTCGCCTTTAGGGATTTTTTGGAAAAC AGGTTTTGGGTTTCTCTGATTCTGAAATCTTGGAGTGGTGTGATCATGACCTTGAGACACCCTGTGGTCA TTAACCAGGGCCCTCAAGTTGGCAAATGCAACATTGATCCTGCTAGTGGGATTTCCATGGTTACTAAATT CGCTGTGGCATGTAGTGGTTTTAAGGATAAGAACCTTCCCCTTACATATAAAGTAATAGTCTCTGAGCTG GACAGCATTGGCGACATCAGTTCTGTAGATGAGAACACACTGGGGACCATTATGTATTTGGGGACACAGC CTACAACACCAGCCTTCTTTCTCCCCGTTGGTATGGCGACAAATCACTACAATTTGAGGCTGTATGCTCA GGTCTATGACTCCTTAGGGACTTTCTCTCAAGTGACCTTGCAAGCCACCGTCAAGGCACCTACCGTCAGG GACTCCTCGAAGAACGTTGTACAACAGCTGCTCAACGTCACGCAACTGAACTCATCTTTGTCCACTTTGC TTCAGAAGCAGGACTGGCTACCGGCAGGTTACTTAGTCTATGTGGTAACTTCAGTCTTCAATAACATAAA ATGGGAGCTCGAACTCCAGGGTGATAGAACCCGCGTCCTGGAGTACCTTGTCAACCAGTCCTTCATGCTT GCTATGAGAACAATGGACGAAATTTGTCAGTCAGTGATGGTGATTACCAAATTAACCCAGAAAGCCTTGG ACTTAAGTCAACCGACTTCAGAGGCCACCACCTTATTGCTAAGGCAAGCAAACCAAGCCCTGCAAGCCTA CCAGCAGGAAAACAAGCACCTTCACTCCCAGCAGATTGAAATTCTGTGCACTGGCATCCTAACCAGCCTG TCTAACCTTCTTAAGCTGGCTAAGACCTACCACACGTCAGAAACTCTTTTGTTTATAATGCAATCATTAT CAGACATGATACTGGCTAACATGGTGCCAGGAAGCAAAACTGTTCTGAGGACCAGCAACTTTAACATGTA TGTGGAGAAAGTTGAAAACTGGAACGTTCATCAGGCCTTCAGAAATGAGTCGCGCTGTTCAAACTGCCTC CGTGCGACACTGAACACAAGCACGGTTCCTGGGCTACCTGCAAAGGCTCCGGTTTCTGTGATGTTTTGCA AGTTCGTAGACGATCCCTTTCCTTGGCTGACTTACCCGGAAAACATCTCTACAGATGTGGTTGGGTTCAG AATGACGGGAGCTGCAGACAACGGTAACGCCATAGAGATTGCACCTGACACAGCAGAAGTGTACCTGGTC AGAAAAGATCTGACCTCTTCCAGTTTTAACCTTACAGTGGGACCAGGCATCAAAAGTGCCGGGTTCTCCA AGATGACAACAGGTGGAATTAGCTTTGAGGTGGACGGCAGGGGGTTTGGGGAGGTACTCATCCACATTGT CACAAACGTGACAGTGTTGTTCGAGGCCTTGGTCTATGAAGGCATTCAGCTCATCCCCACCAATCTAATG GCCATTTTTCTTGTTCCTCATGACATCCCTCCCATTGCCAAGTGGAGTGGCCTGTTTGACCCAAACTGTT CAGTCAAGGAGGCCCGCGTAGTTTGTCTGTCATCAACCCTGCTTCGAACCATAGCTCAGCGCAGACATTC ATTCAAGTACAACATATCGGTGGTCTTGCAGGCCTCTCGCTTTGTCTTTAAGCCCACTAACAAGCTAGTG AGGATTGCTCTCTTTATGGTTCACTGTTTGGACATGTACGGGATCCAGAGTGACTGGGGTCAAAGTACCT GCGTGCTGGGTGAGAGGACGACGTGGAAAACCATACACTGTATCTGTAGGAAGGCAAGGCGGAGCCGGCG GCAGCTGACCTACAGTTATATGCACATTCACTTTGTGACTGCTAAGGTCATTGTGGTCCCTAACCCTGTG GATCTGAGGCTACAGGTCATTAGTAATGTTACCCAGAACCCTGTGACCCTCCTGGCTGTGCTTTGCATAA TGATCGTGTATGTTATTCTGGCTTCCTGGGCCCTGCACAGGGATGTAATAGATCAATACCTTCATGACAA TGTGGTAATACTGACGGATAATGATCCCTTTGATACCATATGCTACCTGGTAACCATCTTTACTGGAAGT CGCTGGGGGTCTGGAACCAAGGCCAATGTTTTTATCCAGCTTATGGGAACGGAGGGTACCAGTGACGCCC ACTGTCTAACCCATCCATATTTTCAAACCCTATACCGGGGAAGTAGGAACACTTTCCTCCTAACGACAAA AAATGACCTGGGGGACATCCATTCCATCCACGTGTGGCATGACAATTCGGGCAAAGCACCCAGCTGGTAC CTAAGTCGAGTCAAAGTGGAAAATCTCTTCAGCAAGCGCATTTGGCTGTTTGTGTGCCGGAGGTGGCTCT CGGTGGACACCACTCTGGAGGAAACATTTCCAGTCACCCACCCGGATGAGTCTCTGAAAAAGACAGACTT CTTCATGATTGATCTGAACGATAAGCTTGGGAAAAACCACATGTGGTTCTCCATTTTCACGGACATCGTC CCCAAACCGTTCAACAGGCTCCAGAGGCTGTCCTGCTGCCTGGCCATGTTGCTAAGCTCTCTCGTTTGTA ATATCATGTTTTTTAACCTGAATATAACGAAGCAACCAGAGTCCAGACAGGGGCATATCATCAGGTCAAT GGTGATAGGAGTCGAAAGTGCCTTAATCACATTCCCTGTGCAGTTATTGATAACTTTTTTCTTCACCTAT TCCCAGAAGAAGCCTAAAGTGGATACAGACAAAGTGGCACCACAGAAGCACCCCCTGATGTCAGAAGAAG GTCTGTCCTGGAAGGATCGGTTAAAAAAATGGCATGAGTATGAAATGAAGACTCTCACTAGTAAAGCTGC CAACCCCGCCTCTGCTCCCGAGGAAAAGGATGCAAACAAGGCCTCTAAAACCTCACAGGCCCCAGACACC CAGACGTCCAATAAGAACTTCAGTAACAATAACCTAGAAGACAGCAAAGATGTCCCTCCGAAAGCTTCCT CTACCCAACTGCACGCTACTCGGTCTGTTAAAAAGACCCAGATTATCCTGCCCAGGCGGTGTGTTTATAT AGCCTGGTTTTTGGTGTTTGCCACGTCTGGTGTATCGTCATTCTTTATTGTATTTTACGGCCTGACTTAC GGCTATGACAAGTCAGTAGAGTGGCTCTTTGCGTCGTTCTGTTCTTTCTGTCAGTCCGTGTTCCTTGTCC AACCGTGTCATATTCTACTGAGGTCAGGCACCAGGATGCACAGGCCCAAGTACTGTAAGAACCTCTCGTG GTCCAGCAAGTACCATTACACAGAAATCAGGCTGCAGGAGCTGAAGATGACCCCAGAAGAGACGGAGCAG CTCCACGAGGACATGGCCTACATCCGAAGCTTGAGCATATACCAGCCCATCACCGAGGACAAAATCCAAA TATTCAGGCGGAAGAACAGGATCAGGAAGAGGTCCTTCTTGTTCCTGAGCTACCTTGTGACCCACTTCAT ATTCCTGACCCTTCTCCTGTTGCTCATTTTCCCCCTGCGTCACAACGATAGCTTCTACTACAACCAGTTT GTTCGCCACCGGTTCTCCATGGATCTTGCCACGGTGGTGAAGCTGGGCGATATCTATGCGTGGCTCAACG GCGTGTTCCTACCTTTGTTACACAATGACAAAAGCCCAACATTCCTTCCGGACAGCTCATCTAAAATCCT TGGCCTTCCACTCATGAGACAAGTGAGGGCCAAACCCAGCAACAAGACATGTCTGTTGGCCAAGAAGTTT GCACAGAGCAGAATTGCAGGGGAACTCCATTGTCATCCACAGTATGGCATTGATCCAGAAGACACAAGAC ACTATCCTGGTGTGTGGAATAAGGCTGGCAAGAGGTCTATAGACAAAACCAGCAATGGCTTTACTTATAA GCCTCCGGGGAGGAAATGGGTGTATTATTCCTATGGCGTATTAAACACCTATGGATCTGGAGGGTATGAG TTCTATTTCTTTCCAGAACAGCAGATGTTTAATTCCACACTGAGGCTCAAGGAACTCGAGGGAAAGAATT GGCTTGATGAGCTGACATGGGCCGTGATTGTGGAGTTGACTACTCTGAATCCAGACACCAGTCTGATGTG TAGCATTTCGGTTGTATTCGAAGTCTCTCCCTTAGGGGTTGTCAACTCCAGCCTCTCCGTGTACTCCTTC CCGCTGGCTGATTTCAACAGCCGCTCTTCGGCAGAAATCTACCTGTATGTAGCCATTCTTATTTTCTTCT GTGCCTATGTCGTGGATGAGGGTTACATCATCATGCAAGAGAGAGCTTCCTACATAAGAAGCATGTACAA CCTGCTCAACTTCTCTCTTAAGTGCATGTTTGCTCTCCTGATAGTGCTTTTCTTCCAGAAGCACCTCCTG GCCACCAAACTGGTCCGGCTTTACAACACTGACCCCGAAGCCTTCATTCCCTTCCATGCTGTCTCTCGGG TTGATCACTTCATGAGGATCATCCTGGCTTTCCTGTTGTTTCTGACCATATTGAAGACACTTAGGTATTC CAGATTCTTCTATAACGTGCGCCTGGCTCAGAAGACCATCCAGGCTGCCTTTCCTGGCATCTGCCACACG GCTTTGGTGGTGTCTATATATTCCTTCATGTACGTAGCTTTTGGGTACCTGGTGTTCGGTCAGCATGAGT GGAATTACAGCAACATGATCCATGCCACCCAGACCATCTTCTCCTACTGCGTGTCAGCCTTTCAGAACAC AGAGTTTTCTGGCAACAAGATCCTCGGGGTCCTGTTCCTGTCATCTTTCATGTTGGTGATGATTTGTATC TTCATCAACTTGTTTCAGGTAGTGATTCTGTCTGCCTATGACGAGATGAAGCAGCCTGTGTATGAGGAGC CGTCTGACGAGGCCGAGGCTGTAACCTATCTGTGCGACAGTCTAAAGTCTGGGTTTGACTTTCTCACCTC CGGACCTCAAGACAAAGACCAATCTGACTTCTTTGAAGACATGCTGTATGGGCAGCCGGAGAAGAATACT CGTCGGTTCTTGGGGCTGAAGGCCAGAAATATCAACGGGAAGAAAATGATTTATCTGGTTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134866
Insert Size	6366 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134866.1, NP_001128338.1
RefSeq Size	6375 bp
RefSeq ORF	6366 bp
Locus ID	300124

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