Spag9 (NM_001108290) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209443 representing NM_001108290 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGAGGACGGTGTGGTATACCAGGAGGAGCCCGGAGGCTCCGGGGCCGTGATGTCAGAGCGGG TGTCCGGCCTGGCCGGCTCTATCTACCGCGAGTTCGAGCGGCTCATCGGGCGCTACGACGAGGAGGTGGT CAAGGAGCTGATGCCACTGGTGGTGGCCGTGCTGGAGAACCTGGACTCGGTGTTCGCGCAGGACCAGGAG CACCAGGTGGAGCTGGAGCTGTTGCGGGACGACAACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGG CGCTGCGCAAGCACGCCGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAGAAAAAGGATTT GCAGACACGAGTGGAGTCTCTAGAATCTCAAACAAGACAACTCGAACTCAAAGCAAAGAACTATGCTGAC CAGATCAGCAGACTTGAAGAGAGAGAAGCAGAACTGAAGAAGGAGTATAATGCACTGCACCAGAGACACA CGGAGATGATCCATAATTACATGGAACACTTGGAAAGAACCAAACTTCATCAGCTCTCAGGGAGTGAGCA GCTAGAGGCCACAGCTCACAGTAGAATTAGAAAAGAGCGTCCTATCTCATTAGGGATTTTCCCTCTACCT GCTGGAGACGGGTTGCTCACACCTGACACTCAGAAGGGAGGCGAGACCCCAGGATCAGAGCAATGGAAAT TTCAAGAATTAAGTCAACCACGGTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAGGGTGG ATCTAAAGCTACCACTCCAGCTTCAACAGCAAATTCGGATGTGTCAGCAATTCCTCCTGATACTCCGTCA AAGGAAGATAATGAAGGACTTGTAAAAGGCACAGATACACCAAATAAGGCAGAGATAAGCAAACACATAG AAGTACAAGTTGCTCAAGAGACTAGGAATGTGTCTACAGATTCTGCCGAAAATGAAGAGAAATCAGAAGT TCAGGCAATCATTGAATCCACTCCCGAACTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGC ACACCCACCAAAGGCATAGAGAACAAAGCTTTCGATCGAAATACAGAGTCTCTCTTTGAAGAACTGTCTT CAGCTGGCTCAGGCCTTATTGGAGATGTGGATGAAGGAGCAGATTTACTAGGAATGGGCCGTGAAGTGGA GAATCTCATACTAGAGAACACGCAGCTGCTGGAAACCAAAAATGCTTTGAATGTAGTGAAGAATGACTTA ATAGCAAAAGTGGATGAGCTGACCTGTGAGAAGGATGTGCTTCAGGGAGAGCTGGAAGCTGTGAAACAAG CCAAGCTGAAGCTAGAGGAAAAGAACAGAGAACTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGA AGATGCAAGGCAAAAAGCAAAAGATGATGATGATAGCGACATTCCTACCGCCCAGAGGAAGCGGTTTACC AGAGTGGAAATGGCCAGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAG CTGTGCGATGGACAGAGATGATCCGGGCCTCCCGAGAAAATCCAGCTATGCAAGAAAAAAAAAGATCAAG CATCTGGCAATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAGCAAAGAAGCCCGAACCCCCTGTT AATCTGAAGTACAATGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTC AGCTCCCTGGTGACAAGTCCAAGGCGTTCGATTTCCTTAGTGAGGAAACTGAGGCTAGCTTAGCCTCCCG CAGAGAGCAAAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCATGTTCAGAAGGAAGATGGCCGAGTGCAA GCTTTCGGTTGGAGTCTGCCTCAGAAGTACAAGCAGGTAGCCAATGGTCAAGGGGAAAATAAGATGAAAA ATTTACCTGTGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGT AGGAGTCAACTTATCCGGCGGCAAGACAAGAGACGGCGGGTCTGTGGTTGGAGCAAGTGTGTTTTACAAG GACGTTTCTGCTCTGGAATCTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGATAAGTTGG ATCAAGAACTCAAGGAACAGCAGAAAGAATTAAAAAATCAAGAAGAATTGTCCAGTCAAGTCTGGATCTG TACCAGCACCCATTCAACTACAAAGGTTATCATCATTGATGCTGTTCAACCTGGCAACATCCTAGATAGT TTCACTGTTTGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGGGCTCGAGAGACAGACTACCCTG CAGGAGAAGAGCTTTCTGACTCTGGTCAGGTAGACAGAGCGTCTCTGTGTGGGAGCATGACCAGTAACAG CTCTGCAGAGATGGACAGCTTGCTGGGAGGCATCACAGTGGTCGGGTGCTCCACAGAAGGAGTGACGGGA GCTGCTGCTTCCCCAAGTACCAATGGTGCTTCTCCGGTGATTGAAAAGCCACCAGAGGTGGAGGCTGAAA ACAGTGAGATTGATGAAAACATTCCAACAGCAGAGGAAGCCACTGAAGCCACAGAGGGCAATGCAGGATC CACTGAAGACGCTGTGGACATCTCCCAGCCTGGCGTGTACACAGAACATGTGTTTACAGATCCTCTGGGA GTTCAGATCCCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAA TATCAGTATTGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAAC CATGTGGCTTGGAGCTCAAAATGGCTGTTTGTATGTCCACTCGTCTGTAGCCCAGTGGAGGAAATGTCTC CATTCCATTAAGCTCAAAGACTCAATCCTCAGTATTGTACACGTGAAAGGAATTGTGCTGGTGGCCCTGG CTGATGGCACCCTTGCAATCTTCCACAGAGGACTTGATGGACAGTGGGACCTGTCAAACTATCACCTCTT AGACCTCGGACGCCCTCATCACTCCATAAGATGCATGACCGTGGTGCATGACAAAGTCTGGTGTGGCTAT AGGAACAAAATCTATGTGGTTCAGCCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGA AGGAGAGCCAAGTACGGCAGCTTGCGTGGGTGGGTGATGGCGTATGGGTTTCCATCCGCTTGGATTCCAC GCTCCGCCTCTATCATGCACATACATACCAACATCTCCAGGATGTGGACATTGAACCTTATGTAAGCAAA ATGTTAGGTACTGGAAAACTGGGTTTCTCTTTTGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTT TGTGGGTGGGGACTGGAAACGGTGTCATTATCTCCATCCCATTGACAGAAACTGTAATCCTCCACCAGGG ACGTTTACTGGGGCTGAGGGCAAATAAAACCTCAGGGACTCCAGGTAATCGTCCTGGAAGTGTAATCCGT GTATATGGTGATGAAAACAGTGACAAAGTGACTCCAGGGACATTTATACCCTACTGTTCGATGGCACATG CGCAGCTTTGTTTCCATGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAGTCCCCGGTCAGGTCGTTAG CCCACAGAGTAGCAGTAGTGGAGCAGATCTAACAGCTGACAAAGCAGGCTCGTCTGCACAGGAGCCCAGT AGCCAGACGCCCTTGAAGTCTATGCTTGTCATCAGTGGAGGAGAAGGCTACATTGACTTCCGAATGGGTG ATGAAGGTGGAGAATCTGAACTTCTTGGAGAGGATCTTCCACTGGAACCTTCAGTCACCAAAGCAGAAAG GAGTCACCTGATAGTGTGGCAGGTGATGTGTGGCAATGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108290
Insert Size	3963 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108290.2, NP_001101760.2
RefSeq Size	7313 bp
RefSeq ORF	3963 bp
Locus ID	360600

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