Scn4a (NM_013178) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	microI; Nav1.4; NCHVS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209327 representing NM_013178 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCATCTCTGCCCAACCTGGTCCCCCCGGGTCCCCACTGCCTGCGCCCCTTCACCCCAGAGT CCCTGGCAGCCATAGAGCAGCGGGCGGTGGAGGAGGAGGCCCGGCTGCAGCGGAACAAGCAGATGGAGAT TGAGGAGCCTGAGCGGAAGCCACGCAGTGACCTGGAAGCTGGCAAGAACCTCCCACTCATCTATGGGGAC CCCCCACCCGAAGTCATTGGCATCCCCCTGGAGGACCTGGATCCTTACTACAGTGACAAGAAGACCTTCA TTGTGCTCAACAAAGGAAAGGCCATCTTCCGATTCTCTGCCACGCCTGCCCTCTACCTGCTGAGCCCCTT CAGCATCGTCAGGAGGGTGGCTATCAAGGTGCTCATTCACGCGCTGTTCAGCATGTTTATCATGATCACC ATCCTGACCAACTGTGTGTTCATGACCATGAGCAATCCGCCTTCTTGGTCCAAACACGTGGAGTACACCT TCACGGGGATCTATACCTTTGAGTCCCTCATTAAGATGCTGGCCCGAGGCTTTTGCATTGATGACTTCAC ATTCCTCCGAGACCCCTGGAACTGGCTGGACTTCAGTGTCATCACAATGGCGTATGTGACAGAGTTTGTG GACTTGGGCAACATCTCAGCCCTGAGGACCTTCCGTGTGCTGCGGGCCCTGAAGACCATCACGGTTATCC CAGGGCTGAAGACAATTGTGGGAGCCCTGATCCAGTCTGTGAAAAAGCTGTCGGATGTGATGATCCTCAC TGTCTTCTGCCTGAGTGTCTTTGCCCTGGTGGGGCTGCAGCTTTTCATGGGAAACCTGCGTCAGAAGTGC GTGCGTTGGCCCCCGCCCATGAATGACACCAACACCACGTGGTATGGCAATGACACTTGGTACAGCAATG ACACTTGGTACGGCAATGACACTTGGTACATCAATGACACTTGGAACAGCCAGGAGAGCTGGGCCGGCAA CTCTACCTTTGACTGGGAGGCCTACATCAATGACGAAGGGAACTTCTATTTCTTGGAGGGCTCCAATGAT GCTCTGCTCTGTGGGAATAGCAGTGATGCTGGGCACTGCCCTGAGGGCTACGAATGCATAAAGGCTGGGC GGAACCCCAACTATGGCTACACCAGCTATGACACCTTCAGCTGGGCTTTCCTGGCTCTCTTCCGGCTCAT GACGCAGGACTACTGGGAGAACCTTTTCCAGCTGACCCTACGAGCTGCTGGCAAGACCTACATGATCTTC TTCGTGGTCATCATCTTCCTGGGCTCCTTCTACCTCATCAATCTGATCCTGGCCGTGGTGGCCATGGCGT ACGCTGAGCAGAATGAGGCTACCCTGGCCGAAGACCAGGAGAAAGAGGAGGAGTTCCAACAGATGCTTGA GAAATACAAAAAACATCAGGAGGAACTGGAAAAGGCTAAGGCTGCCCAGGCTCTGGAAAGTGGAGAGGAG GCAGATGGGGACCCAACCCACAACAAAGACTGCAATGGGAGCCTGGATGCATCCGGGGAGAAGGGGCCCC CAAGGCCAAGCTGCAGCGCAGACAGTGCCATCTCAGATGCTATGGAGGAGCTGGAAGAGGCCCATCAGAA GTGCCCACCGTGGTGGTACAAGTGTGCACACAAAGTCCTCATCTGGAACTGCTGTGCCCCGTGGGTGAAG TTCAAACATATAATCTACCTGATCGTCATGGACCCCTTTGTGGACCTGGGTATCACCATCTGCATTGTGC TCAACACCCTCTTCATGGCCATGGAGCACTACCCCATGACCGAGCACTTTGACAACGTGCTCTCCGTGGG CAACTTGGTCTTCACAGGCATCTTCACTGCGGAGATGGTGTTGAAGCTGATTGCCATGGACCCCTACGAG TATTTCCAACAGGGCTGGAACATCTTTGACAGTTTCATCGTCACCCTCAGCCTGGTGGAGCTGGGCCTGG CCAACGTACAGGGGCTGTCAGTGCTCCGTTCCTTCCGCCTGCTGCGTGTCTTCAAGCTGGCCAAGTCATG GCCAACACTCAACATGCTCATCAAAATCATTGGCAACTCAGTGGGCGCGCTGGGCAACCTGACCCTGGTG CTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTGTTCGGCAAGAGCTATAAGGAGTGTG TGTGCAAGATCGCCTCAGACTGCAACCTGCCTCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTCAT CGTCTTCCGCATCCTCTGCGGGGAATGGATCGAGACCATGTGGGACTGCATGGAGGTGGCCGGCCAGGCC ATGTGCCTCACCGTCTTCCTCATGGTCATGGTCATTGGCAACCTGGTGGTCCTGAATCTGTTCCTGGCTC TCCTGCTGAGTTCCTTCAGTGCTGACAGCCTGGCGGCCTCGGATGAGGATGGCGAGATGAACAACCTACA GATTGCCATCGGGCGTATCAAGTGGGGCATCGGTTTTGCCAAAACCTTCCTCCTGGGGCTGTTGCGTGGC AAGATCCTGAGCCCCAAGGAAATAATACTCAGCCTCGGTGAGCCCGGGGGTGCCGGGGAAAATGCCGAGG AGAGTACTCCCGAGGATGAGAAGAAGGAGCCGCCACCGGAAGATAAGGAGCTGAAAGACAATCATATCCT GAACCACGTGGGCCTGACCGATGGCCCCCGCTCCAGCATCGAGCTGGACCACCTTAACTTCATCAACAAC CCCTACCTCACCATCCAGGTGCCCATTGCCTCCGAGGAGTCTGACCTGGAGATGCCCACAGAGGAGGAGA CAGACGCCTTCTCGGAGCCTGAGGATATCAAGAAGCCCCTACAGCCCCTCTACGACGGGAACTCCTCCGT CTGCAGCACAGCTGACTACAAGCCCCCTGAAGAGGACCCCGAGGAGCAGGCTGAGGAGAACCCCGAGGGG GAGCAGCCTGAGGAATGCTTCACGGAAGCCTGTGTGAAGCGCTGCCCCTGCCTCTATGTGGACATCTCCC AGGGCCGTGGGAAAATGTGGTGGACACTGCGCAGGGCCTGTTTCAAGATTGTTGAGCACAACTGGTTTGA GACCTTCATTGTCTTCATGATCTTGCTCAGCAGTGGAGCCCTGGCCTTCGAGGACATCTACATTGAACAG CGACGAGTCATCCGCACCATCCTGGAATACGCCGACAAGGTCTTCACCTACATCTTCATCCTGGAGATGT TGCTAAAGTGGGTCGCCTACGGCTTCAAGGTGTATTTCACCAATGCCTGGTGCTGGCTCGACTTCCTCAT CGTGGACGTCTCCATCATCAGCCTGGTGGCAAACTGGCTGGGCTACTCTGAGCTGGGGCCCATCAAATCC CTGCGCACACTCCGGGCCCTGCGACCTCTGAGGGCACTGTCTCGATTTGAGGGTATGAGGGTGGTGGTGA ACGCCCTCCTGGGAGCTATCCCCTCCATCATGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTT CAGCATCATGGGGGTCAACTTGTTTGCTGGAAAGTTCTACTACTGCGTCAACACCACCACCTCTGAGAGA TTCGACATCTCGGTGGTCAACAACAAGTCCGAGTCTGAGAGCCTCATGTATACAGGCCAGGTCCGCTGGA TGAATGTCAAGGTCAACTACGACAACGTGGGTCTGGGCTATCTCTCCCTCCTTCAGGTGGCCACATTCAA GGGTTGGATGGATATCATGTATGCAGCTGTGGACTCCCGGGAGAAAGAGGAGCAGCCACACTATGAGGTG AACCTCTACATGTACCTCTACTTCGTCATCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTCTTCA TCGGTGTCATCATCGACAACTTCAACCAACAGAAGAAGAAGTTTGGAGGGAAAGACATCTTCATGACAGA GGAACAGAAGAAATACTACAATGCGATGAAGAAGCTTGGCTCCAAGAAGCCCCAGAAGCCAATTCCCCGG CCTCAGAACAAGATCCAGGGCATGGTGTACGACTTCGTGACGAAGCAGGTGTTTGACATCTCCATCATGA TCCTCATCTGCCTCAACATGGTTACTATGATGGTGGAGACAGATGACCAGAGCCAGCTCAAGGTGGACAT CCTGTACAACATCAACATGGTCTTCATTATCATCTTCACAGGGGAGTGTGTGCTGAAGATGTTTGCCCTA CGCCATTACTACTTCACCATTGGCTGGAACATCTTTGACTTTGTGGTTGTCATCTTGTCCATCGTGGGCC TTGCGCTCTCTGACTTGATACAGAAATACTTTGTGTCACCCACGCTGTTCCGTGTGATCCGCTTGGCTCG GATCGGGCGTGTCCTGCGTCTGATCCGCGGGGCCAAGGGCATCCGGACGCTGCTGTTCGCCCTGATGATG TCCCTGCCCGCCCTCTTCAACATCGGCCTCCTTCTCTTTCTGGTCATGTTCATCTACTCGATCTTCGGCA TGTCTAACTTCGCCTACGTCAAGAAAGAGTCAGGCATCGACGACATGTTTAACTTTGAGACCTTTGGCAA CAGTATCATCTGCCTCTTCGAGATCACCACGTCAGCCGGCTGGGACGGGCTTCTGAACCCCATCCTCAAC AGTGGGCCCCCAGACTGTGACCCGACGTTGGAGAACCCGGGTACCAATGTCAGGGGGGACTGCGGCAACC CTTCCATCGGCATCTGTTTCTTCTGCAGCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACAT TGCTATCATCCTGGAGAATTTCAACGTGGCCACCGAAGAGAGCAGCGAGCCCCTCAGTGAGGATGACTTC GAGATGTTCTATGAGACCTGGGAGAAGTTCGACCCGGACGCCACTCAGTTCATCGACTACAGCCGCCTCT CGGACTTTGTGGACACCCTGCAGGAGCCACTGAAAATCGCCAAACCCAATAAGATCAAGCTTATCACGTT AGACCTGCCCATGGTGCCCGGCGACAAGATCCACTGCTTGGACATCCTCTTTGCCCTGACCAAAGAGGTA CTGGGTGACTCTGGGGAGATGGACGCCCTCAAGCAGACCATGGAGGAGAAGTTTATGGCAGCTAACCCTT CCAAGGTCTCCTATGAGCCCATCACCACCACCCTCAAGAGGAAGCAGGAGGAGGTGTGTGCTATCAAAAT CCAGAGGGCCTACCGCCGCCACCTGCTGCAGCGCTCCGTGAAGCAGGCCTCTTACATGTACCGTCACAGC CAGGACGGCAATGACGACGGGGCCCCCGAGAAGGAGGGATTGCTTGCCAACACCATGAACAAGATGTATG GCCATGAGAAAGAGGGTGATGGTGTGCAGAGCCAGGGGGAGGAAGAGAAGGCCTCTACAGAGGATGCTGG ACCCACCGTGGAGCCCGAACCCACCAGCAGCTCAGATACTGCCCTGACTCCTTCTCCTCCACCACTACCC CCCTCATCATCACCGCCACAAGGGCAGACAGTTCGCCCAGGGGTCAAAGAGTCTCTTGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013178
Insert Size	5523 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013178.1, NP_037310.1
RefSeq Size	6957 bp
RefSeq ORF	5523 bp
Locus ID	25722
UniProt ID	P15390
Gene Summary	voltage-gated Na+ channel subunit [RGD, Feb 2006]

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