Afdn (NM_013217) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Af6; Mllt4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209209 representing NM_013217 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGGCGGCCGCGACGAAGAGCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACC GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTT TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGCACAGCAACCACTCAAGAT GTCATTGAAACACTGGCAGAGAAGTTCCGCCCTGATATGCGCATGCTCTCCTCTCCCAAGTACTCCCTCT ATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAAACCTCTAGTTGTACAGTTGAATTG GAACAAAGATGATCGGGAGGGTAGATTCGTCCTTAAGAACGAGAATGACGCCATTCCTGCCAAGAAGGCT CAGAGTAATGGACCTGAGAAGCAGGAGAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTGTCAAAGA AAGAAAAGAAGGAAAAAAAGAAGAGAGAGAAGGAGGCTTTGAGACAAGCCTCTGATAAGGAAGAAAGGCC TTCTCAAGGGGATGACAGTGAGAATTCTCGTCTGGCTGCTGAAGTTTACAAAGACATGCCTGAAACCAGC TTTACTCGAACGATTTCTAATCCTGAAGTTGTTATGAAACGGCGGCGGCAGCAAAAACTGGAGAAGAGAA TGCAAGAGTTTCGGAGCTCAGATGGGCGGCCCGACTCGGGTGGAACACTGAGAATCTATGCAGACAGTTT AAAACCAAATATTCCATACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCTGTAGCAGAG TCCTTAGAGAAGTATGGTCTGGAAAAAGAGAACCCCAAGGACTACTGCATTGCTCGGGTTATGCTTCCTC CTGGAGCCCAGCATTCTGATGAGAGAGGTGCTAAGGAAATAATTCTTGATGACGATGAATGCCCTTTACA AATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGACTAC ATTCCAAAGAAAATGAAGAAACATGTTGAAGGCAAGCCATTGAAGGGGAAAGATAGAGCTGACGGGTCTG GCTACGGCTCTGCTCTTCCTCCTGAGAAGCTGCCCTACTTAGTAGAGCTCAGCCCAGGGAGAAGGAATCA CTTTGCCTACTACAGCTATCACACTTACGAAGATGGCTCTGACTCCAGAGACAAGCCAAAGTTATACCGC CTTCAGTTAAGTGTGACTGAAGTTGGGACAGAGAAGTTCGATGACAATTCTATTCAGCTGTTTGGTCCTG GAATTCAGCCCCATCACTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGA TGCAGAGACCTATGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTACAGAGTGGCATGAGACTGCAG TTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTCCAAGAGATCTGTGG ATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCCGTTCAAGAGACAACCTTTGAATTGGG AGGAGATATCCACAGTGGGACAGCATTGCCGGCAAGCAGGAGCACTACTAGATTGGACAGTGACAGGGTG TCCTCTGCCTCTAGCACAGCTGAGCGAGGGATGGTGAAGCCAATGATCCGACTGGACCAGGAGCAGGACT ACCGGCGAAGAGAAAGCAGGACTCAGGATGCTGCTGGGCCTGAACTGATGCTGCCTGCCAGCATTGAATT CCGGGAAAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGCTCTACAGTTCATTTT AAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCAGCACAGGCCCGACA TCAGTCCTACAGAGCGGACACATAAGGCCATTGCTGTTGTGAACAAGATGGTGAGCATGATGGAAGGGGT CATCCAGGAAGTAGACCAGGTTGATCAGAAACAGAAGAACATTGCAGGAGCACTTGCTTTCTGGATGGCA AATGCATCTGAGCTTCTCAACTTCATCAAGCAGGACCGAGACCTTAGTCGGATTACTCTGGATGCCCAGG ATGTTCTAGCACACTTGGTTCAGATGGCATTTAAGTACTTGGTTCACTGCCTTCAGTCAGAACTTAATAA CTACATGCCAGCCTTTCTGGATGATCCCGAGGAGAACAGTCTGCAGAGACCAAAAATAGATGATGTGCTG CATACGCTCACAGGAGCCATGTCCTTGTTGCGGCGCTGCAGAGTCAATGCCGCCTTGACCATCCAGCTGT TCTCTCAGCTTTTCCACTTCATCAATATGTGGCTATTCAACAGACTGGTGACTGACCCAGATTCAGGCCT GTGTTCCCACTACTGGGGCGCAATTATCCGCCAGCAGCTGGGGCATATTGAAGCTTGGGCAGAGAAGCAG GGACTAGAGTTGGCAGCTGACTGTCACTTGAGCAGGATCGTGCAGGCTACTACGTTGCTTACAATGGATA AGTATGTACCTGATGACATTCCAAATATAAACAGCACATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGC CCTCTTACAGAATTACCACTGTGCACCTGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTCGTG GCTGTTGCTGAGAACACAGCTGATGAGCTTGCCCGCAGTGATGGCAGAGATGTGCAGTTAGAGGAGGACC CTGATCTACAACTGCCCTTTCTTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCCAAA TGGTTTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGCAGGTTGGTTCCTCACACACGTTCT CCAGGTACCTGGACAATATATTTTGAAGGAGCAGATTATGAAAGTCACCTCATGCGTGAGAACACAGAAC TGACCCAGCCTCTGAGGAAGGAGCCTGAGGTAATCACTGTGACCCTGAAGAAGCAGAATGGAATGGGCCT CAGCATTGTTGCAGCAAAGGGTGCTGGCCAGGATAAACTGGGAATCTACGTTAAGTCCGTTGTCAAAGGA GGTGCTGCAGATGTGGATGGACGCCTAGCTGCTGGTGACCAACTCCTTAGTGTGGATGGACGAAGTCTGG TTGGACTTTCTCAGGAAAGGGCAGCAGAACTCATGACAAGAACCAGTTCCGTGGTAACACTGGAAGTTGC AAAGCAAGGCGCTATCTATCACGGACTGGCCACTCTCCTTAACCAGCCATCACCCATGATGCAGAGAATT TCAGATCGTCGTGGCTCAGGTAAACCCAGACCAAAGAGCGAAGGTTTTGAGCTCTATAATAATTCAGCTC AAAACGGCTCCCCAGAGAGTCCTCAGATGCCTTGGACAGAGTACAGTGAGCCAAAGAAGCTGCCTGGTGA CGACAGGCTGATGAAAAACAGAGCTGACCACCGTTCCAGCCCCAATGTGGCAAATCAGCCCCCTAGTCCT GGAGGGAAGAGTCCATATACCTCTGGAACAGCAGCTAAAATAACATCTGTCTCTACTGGAAACCTCTGCA CTGAGGAGCAGACCCCTCCACCTAGACCTGAAGCCTATCCTATCCCTACTCAGACATATACCAGAGAGTA TTTTACCTTTCCAGCTTCAAAGTCGCAGGATCGGATGGCTCCTGTTCAAAACCAGTGGCCAAATTATGAG GAAAAACCACACATGCACACAGAAAGTGATCATGCCAGTATTGCAATCCAGCGTGTTACCCGTTCCCAGG AAGAGCTTCGGGAAGAGAAGGTTTACCAACTTGAGCGTCATCGAGTAGAGTCAGGCATGGATCGCAAGTG TGACAGTGATATGTGGATCAATCAGAGCTCCTCAGTGGAATCTAGCACGTCTAGCCAGGAGCACCTGAAC CATTCCTCCAAGTCAGTTACCCCTGCTTCCACTCTGACCAAGAGTGGTCCTGGTCGTTGGAAAACCCCAG CAGCTGTGCTCCCCACCCCTGTGGCTGTCTCCCAACCCATTCGAACAGACCTCCCTCCACCACCACCTCC ACCACCTGCCCACTACACCAGTGATTTTGATGGTATTTCAATGGATTTGCCCCTCCCACCTCCTCCTGCC AACCAAGCAGCTCCCCAGTCTGCTCAGGTGGCTGCTGCAGAGAGGAAAAAGAGAGAGGAGCATCAGAGGT GGTATGAAAAAGAGAAGGCCCGCCTGGAGGAGGAACGAGAGAGAAAGCGCAGGGAGCAAGAGAGGAAGTT GGGGCAGATGCGCACTCAGTCCCTGAACCCTGCTTCCTTCTCTCCTTTGGCCACACAGGCCAAGCCAGAA AAGCCTTCCACACTGCAGAGGCCCCAAGAAACGGTCATTCGGGAGCTGCAGCCCCAGCAGCAGCCCCGTA CTATTGAGCGCAGGGATCTGCAGTACATAACCATCAGTAAAGAGGAGCTGTCCTCTGGGGATAGTCTGTC TCCAGACCCATGGAAACGAGATGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCACATTGTAGACATG CTAAGCAAGGAGATCCATGAGCTGCAAAACAAGGGGGACCGTACTGCAGAGGAGAGTGACCGCCTGCGGA AGCTTATGCTGGAGTGGCAGTTCCAGAAGAGACTACAGGAGTCCAAGCAGAAGGATGAGGATGATGATGA AGAGGAAGATGATGATGTTGACACCATGCTGATCATGCAGCGACTGGAGGCGGAGCGGAGAGCCAGGTTG CAGGATGAAGAGCGCAGGCGCCAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGTTGAAGACAGAGTAA GACAAGAGGAAGATGGACGCCATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCGACA GGAAGAAGGGTATTACAGCCGCCTAGAAGCTGAGAGGCGCAGACAGCATGAAGAGGCAGCACGCAGGTTG CTGGAGCCCGAAGAACCCGGGCTGAGCCGACCTCCACTTCCACAGGACTACGAGCCCCCATCCCAGTCCT CAGCACCCAGTGCCCCTCCTCCCCCACCTCAGCGAAATGCCTCCTACCTCAAAACACAGGTCCTCTCCCC AGACTCGCTGTTCACTGCCAAGTTTGTTGCGTATGATGATGATGATGAGGAGGAGAACTACGTCCCAGCA GGACCAAACTCTTATTCGGGATCTGCTGGCACAACTGCTGGAACTTATGATGCTCCTCGGGACACAAGAG AAAAACTCAGTAGAAGCCAAGATGCAGACTTACCTGGCAGTTCTGGAGCCCCTGAAAACCTGACATTTAG AGAGCGTCAGCGCCTCTTTTCACAAGGTCAAGATGTGTCAGACAAAGTGAAAGCTTCTCGTAAATTAACA GAGCTTGAGAATGAACTCAACACCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013217
Insert Size	5490 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013217.2, NP_037349.1
RefSeq Size	7656 bp
RefSeq ORF	5490 bp
Locus ID	26955
UniProt ID	O35889
Gene Summary	may play a role in linking the actin cytoskeleton to the plasma membrane [RGD, Feb 2006]

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