Map3k6 (NM_001107909) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209109 representing NM_001107909 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCCGTGTCCCGGGGCCGGGGTTCTAGAACGGGCGGGCAGCTGCTGGCAGGACCCTCTGGCGG AGGCGCTGAGCCGGGGCCGCTCGTCTCCTGCGGTCGCCGGTCGGGGCTGCGCGCGGAGCCGGCCTCTCAG TGTGGTCTACGTGTTGACCCGCGAGCCTGGACCCGGGGTGGAGCCCGGGTCGGGAACCGAGGCAGAGCCG CTGCCGCTGCGATGCCTGCGCGAGGCCTGTGCTCAGCTCCAGAGCACGCGGCCACCCCCGCAACTGCGCA GCCTGCCCTTTGCGACGCTGGCGCTAGGCGACACCGCTGCGCTAGACTCCTTCTACAACGCGGACGTAGT GGTGCTGGAGGTGAGCAACTCCCTGGCACAGCCTTCCTTGTTCTACCACCTCGGCGTGCGTGAGAGCTTC AGCATGACCAACAATGTGCTCCTCTGTTCCCAGGCAGAGCTCCCTGACCTGCAGGCCCTTCGTGAGGATG TTTTCCAGAAGAACTCGGATTGCGTTGGCAGCTACACACTGATTCCTTATGTTGTGACAGCCACTGGCCG GGTCTTATGTGGCGATGCAGGCCTCCTGAGGGGCATAGCCGATGGGCTAGTACAGGCTGGGGTGGGCACT GAGGCCCTCCTCACGCCCCTGGTGGGCCGGCTTGTCCGTCTCTTGGAGGCCACACCCACAGACTCTTGCG GCTACTTCCGGGAGACCATTCGTCAAGATATCCGGAAGGCACGGGAGCGGTTCAGCGGGCAGCAGCTGAG GCAGGAGCTGGCTCGCCTGCAGCGGAGGCTGGACAGCGTAGAGCTGTTGAGCCCCGACATCATCATGAAT CTGCTTCTGTCATACCGCGACGTCCAGGACTACTCCGCCATCATTGAGCTGGTGGAGACGCTGCAGGCTT TGCCCACCTGTGATGTGGCCGAACAGCACAATGTCTGCTTCCACTACACGTTTGCCCTCAACAGGAGGAA CAGGCCTGGGGACCGTGAGAAGGCCCTGGCTGTCCTTCTGCCATTGGTGAAGCATGAAGGCCCCGTGGCT CCAGACCTGTACTGCATGTGTGGTCGTATCTACAAGGACATGTTCTTCAGCTCCAGCTTCCAAAGTACTG GGCACCTGGAGCAGGCCTATCACTGGTACCGCAAGGCATTTGATGTGGAGCCCAGCCTACACTCGGGCAT CAATGCAGCTGTGCTGCTCATTGCAGCTGGGAAACACTTTGACGACTCTGAGGAGCTCCAGCTGATAGGC ATGAAGCTGGGCTGCCTGTTAGCCCGCAAAGGCTGTGTGGAGAAGATGCAGTATTACTGGGACGTGGGGT TCTACCTGGGAGCCCAGATTCTTGCCAACGACCCCATCCAGGTGGTGCTGGCTGCAGAACAACTATACAA GCTCAATGCCCCCATATGGTACTTGGTGTCAGTGATGGAGACCTTCTTACTGTACCAGCATTTCAGACCC ACACCAGAGCCCTCGGGAGGACCCGTGCTGCGAGCTCACTTTTGGCTCCATTTCTTGCTACAATCCTGCC AGCCTTTCAAGGCGACCCCTCCTCAGGAGGACCAGTGCCTGGTACTGGTGCTGGAGATAAACAAGGTTCT GCTGCCTGCCAGACTTGAGATTCAGGGAGCCGACCCCATGAGCGCAGTGACCCTAAGCTTGCTGGAGCCA GAGACTCAGGATGATCCTTCCAGCTGGACCTTCCCAGTCACCTCCATCTGTGGGATCAGCGCCTCCAAGC TGGACCAGCGCTGCTGCTTCCTGTACGCACTTCCTCCGGCCCAGGACGTCCAGCTGTGCTTTCCCAGCGT AGAGCGCTGCCGGTGGTTCTGTAGCCAAATTCAGGTCTTGGTGATGAATCCAGATTCCTCGGCGCCCACT GAGGAGGCAGAAGGCACAAGGGAGGTGCTGGAGTTTGATTATGAATATTCGGAGACGGGTGAGCGGCTGG TGCTGGGCAAAGGCACGTACGGGGTGGTGTATGCTGGCCGCGACAGGCACACGAGGGTACGAATCGCCAT CAAAGAGATCCCAGAGAGAGATAGCAGATTCTCTCAGCCTCTGCACGAGGAGATCGCTCTCCACAAACGC CTTCGCCACAAGAATATAGTGCGCTATTTGGGCTCAGCCAGCCAGGGCGGCTACCTCAAGATCTTCATGG AGGAAGTGCCTGGAGGCAGCCTGTCCTCCTTGCTTAGGTCAGTGTGGGGACCCCTAAAGGACAACGAGAG TACTATTAGCTTCTACACGCGTCAGATCCTGCAAGGACTTAGCTACCTGCATGAGAACCGCATTGTTCAC CGAGACATCAAGGGGGACAATGTACTGATCAACACCTTCAGTGGGCTGCTCAAGATTTCTGACTTTGGCA CCTCCAAGCGGCTGGCAGGCATCACACCCTGCACGGAGACTTTCACAGGGACCCTGCAGTATATGGCCCC AGAAATCATTGACCAGGGCCCACGAGGATATGGAAAGGCGGCTGACATCTGGTCTCTGGGCTGCACTGTG ATTGAGATGGCCACAGGTTGGCCGCCCTTTCACGAACTAGGGAGCCCGCAGGCTGCCATGTTTCAGGTGG GCATGTACAAGGTACATCCACCAGTGCCAAGTTCCCTGTCAGCTGAGGCCCAAGCCTTCCTCCTCCGAAC TTTTGAGCCAGATCCCCGCCTCCGAGCCAGTGCCCAAGAGCTGTTGGGAGACCCCTTCCTGCAACCAGGG AAGAGGAGCCGCAGCCCTGGCTCTCCTCGGCACACTCCCCGGCCCTCAGGCACCCCTTCCAGTACTTCCA GTCCTTCAGCTGACTCAGCCACACAGTCTCAGACATTCCCAAGGCCCCAGGCACCCTCTCAGCACCCACC CAGCCCTCCGAAGCGCTGCCTTAGTTATGGGGACACCAGTCAGCTCCGTGTGCCCGAGGAATCCGCTGCC GAGGAACCCGCGTCCCCAGAAGAGAGTTCAGGCCTGAGCCTGCTGCACCAGGAGAGCAAGCGCCGAGCCA TGCTGGCTGCAGTGCTGGAACAGGAGGTGCCCATACTAGCAGAGAATCTCCTGGATCAGGAACAGGATTC TCGACTCAGCAGGAATCATGTGGAACTTCTGCTTCGGTGCCTCGGGGCACAAATCCATACTCCTAACCGC CGGCAGCTGGCCCAGGAGCTGCGGGCCCTGCAAGCTCAACTGCGGGCCCAGAGCCTGAGCCCTGCGCTTT TGAAGGGGCCGCTCTTCGCCTTTCCAGACGCGGTGAAGCAGATCCTCCGCAGACGCCAGATCCGCCCACA CTGGATGTTCGTGTTGGACTCGCTGCTCAGCCGGGCAGTCCGGGCGGCGCTGGCCGTACTGGACGCGGAG GCAGAGAAGAAAGCGGTCTCACCGAAGTCAGAGGAGTGGAGTCAAGGGGAGTCCCAGCAGAAACCGCTGG AGAGCCAGCCGCCGCTGCAGAGTCAGCTCCCACCAGAGCAGGAACCTCCATCCCTGATGATACAGCTGGG TCTCTTGCGAGCTGAGACTGACAGGCTTCGGGACATTCTGGCTGAGAAGGAACGTGAGTGCCAGGCCCTC GTGCAACAGGCCCTGCATCGGGTGCACGCAGAGACCAGGACGTATGCCCTGGCTTCAGAGACCCCGGCCA CTCTCCCAAAGGACCAGAAACTGGTGCAGTGGCTACAGGACCTGAGTGTAGATCCAACCACTATCCAAAC GCTCCTGAGCCATAGCTTTACCCTTCAAACCCTGCTCACCTGTGCCACTCAGGATGACCTCGTCTACACC AGAATCAGGGGAGGGATGGTATGCAGAATTTGGAGAGCCATCTTGGCACAGCGAGCAGGAGCCACGTCGG TCACCCCTGGACCCCGGGATGCTGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001107909
Insert Size	3879 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107909.1, NP_001101379.1
RefSeq Size	4339 bp
RefSeq ORF	3879 bp
Locus ID	313022

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