MAST1 (NM_181089) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sast
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209101 representing NM_181089 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACTCTCTCTGGACCGCGCTTTCCAATTTCTCGATGCCCTCCTTCCCCGGCGGCAGCATGTTTC GCCGCACCAAGAGCTGCCGTACCAGTAACCGGAAAAGCCTCATCCTGACAAGCACTTCTCCGACGCTACC GCGACCCCACTCCCCGCTGCCGGGTCACTTAGGTAGCAGTCCCCTGGACAGCCCCCGCAACTTCTCTCCC AACACCCCTGCCCATTTCTCGTTTGCTTCTTCCCGAAGGGCGGATGGACGCCGGTGGTCCCTTGCTTCCC TTCCTTCCTCTGGATATGGCACCAACACCCCAAGCTCAACCGTCTCATCCTCCTGCTCCTCCCAGGAGCG CCTGCATCAACTACCCTACCAGCCCACCGTGGACGAGTTGCACTTTCTGTCCAAACACTTCGGCAGCACC GAGAGCATCACCGACGAAGATGGTGGCCGCCGCTCCCCCGCCGTGCGTCCTCGCTCTCGGAGCCTCAGCC CCGGACGCTCCCCCTCCTCCTACGACAACGAAATCGTGATGATGAACCATGTGTACAAGGAGCGGTTCCC GAAGGCCACCGCGCAGATGGAAGAGAAGCTGCGGGACTTCGCCCGCGCCTACGAACCTGACAGCGTGCTG CCGCTGGCCGACGGCGTGCTCAGCTTCATCCACCACCAGATCATTGAACTGGCCCGAGACTGCCTTACCA AGTCCCGCGATGGGCTCATCACCACGGTCTATTTCTATGAACTGCAGGAAAACCTAGAGAAGCTGCTGCA GGACGCCTACGAACGCTCGGAGAGCCTGGAGGTGGCCTTCGTCACTCAGCTGGTGAAGAAACTGCTCATC ATCATCTCTCGCCCTGCAAGGCTTCTGGAGTGCCTGGAATTCAACCCAGAAGAATTCTACCACCTGCTGG AGGCAGCGGAAGGCCACGCCAAGGAGGGCCACCTGGTCAAAACTGACATCCCCCGATACATCATCCGGCA GCTGGGCCTCACTCGGGACCCCTTTCCAGATGTGGTGCGCCTGGAGGAGCAGGACAGCGGGGGCTCCAAC ACGCCTGAACAAGATGACACGTCTGAGGGTCGCAGCAGCACCTCCAAGGCCAAGAAACCTCCGGGTGAAA GTGACTTTGACACTATCAAGCTCATAAGCAATGGTGCCTACGGGGCTGTCTACCTGGTGCGGCACCGGGA CACACGGCAGCGCTTTGCCATGAAGAAGATCAACAAACAGAACTTGATCCTTCGCAACCAGATCCAGCAG GCGTTCGTGGAGCGTGACATCCTCACGTTTGCGGAGAACCCGTTTGTGGTCGGCATGTTCTGCTCCTTTG AGACCCGGCGCCATCTTTGCATGGTCATGGAGTATGTGGAAGGTGGTGACTGTGCCACTCTCCTGAAGAA CATTGGGGCACTGCCTGTGGAGATGGCTCGAATGTATTTTGCAGAAACGGTTCTAGCTCTTGAATACTTA CACAATTATGGCATTGTGCACCGTGACCTCAAGCCAGACAACCTCCTCATCACTTCCATGGGCCACATCA AACTCACGGATTTTGGCCTCTCCAAGATGGGACTCATGAGCCTCACCACCAACTTATATGAAGGTCACAT TGAGAAAGACGCCCGGGAGTTCCTGGACAAACAGGTGTGTGGGACCCCGGAGTACATCGCACCCGAGGTC ATCCTGCGCCAGGGCTACGGCAAGCCGGTGGACTGGTGGGCTATGGGCATCATTCTCTACGAGTTCCTGG TGGGCTGTGTGCCTTTCTTCGGGGATACCCCAGAAGAGCTCTTTGGACAGGTCATCAGTGACGACATATT GTGGCCTGAAGGGGATGAGGCCCTGCCCACAGACGCACAGCTTCTAATATCTAGCCTCTTGCAGACCAAC CCCCTGGTCCGGCTTGGGGCAGGCGGTGCTTTTGAAGTAAAGCAGCATAGCTTCTTCCGAGACCTAGACT GGACGGGGCTACTGAGGCAGAAGGCAGAGTTCATCCCCCACCTAGAGTCAGAAGATGACACCAGCTACTT TGACACCCGCTCCGACAGGTATCACCATGTGAACTCTTATGATGAGGACGACACCACTGAGGAGGAGCCC GTGGAGATCCGCCAATTCTCCTCCTGCTCGCCGCGCTTCAGCAAGGTGTACAGCAGTATGGAGCAGCTGT CTCAGCACGAGCCCAAGACTCCCGTCTCAGCATCTGGGGCTAGCAAGCGGGACCCAAATGCTAAGGGCCC GGAGGAGAAGGTGGCCGGAAAGAGGGAGGGACTGGGTGGCCTGACTCTGCGTGAGAAGACCTGGAGAGGG GGCTCTCCAGAGATTAAACGCTTCTCAGCATCTGAAGCCAGTTTTCTGGAGGGAGAGGCCAGTCCCCCGC TGGGAGCGCGCAGACGGTTCTCAGCGCTACTGGAGCCCAGCCGCTTCACTGCCCCTCAGGAGGATGAAGA TGAGGCCCGACTCCGCAGACCTCCTCGGCCCAGCTCTGATCCCCCTAGCTCACTGGACACACGCGTCCCC AAAGAAGCAGCACAAGGAGAGGGCACATCCACCCCGGGAGAACCCGAGGCCACTGAGCGCTCACATCCAG GTGACTTCTGCCCACCCTCCAAGGATGGAGACCCGTCAGGTCCTAGGGCCACCAATGATCTGGTTTTGCG AAGGGCGAGGCACCAGCAGCTGTCTGGGGATCTCTCGGTGGAGAAGCGACCGTCTCGAACCGGGGGCAAA GTTATCAAGTCGGCGTCAGCTACAGCCTTGTCGGTCATGATTCCTGCAGTGGACCCACACGGAGGCTCAC CCCTGGCTAGTCCCATGTCCCCTCGATCTCTCTCCTCCAATCCATCCTCTCGGGACTCTTCGCCTAGCCG AGACTACTCACCAGCTGTCAGTGGACTCCGCTCCCCCATCACCATCCAACGCTCCGGCAAGAAATATGGC TTCACACTACGAGCCATCCGTGTCTACATGGGCGACTCAGATGTCTACAGTGTCCACCACATTGTTTGGC ACGTGGAGGAAGGAGGTCCAGCCCAAGAGGCAGGGCTCTGTGCTGGAGACCTCATCACTCACGTGAACGG TGAGCCTGTGCACGGTATGGTGCACCCTGAGGTGGTGGAGCTGATTCTAAAGAGTGGCAACAAAGTAGCT GTGACCACCACACCCTTTGAAAACACTTCCATCCGGATTGGGCCGGCCAGGCGCAGCAGCTACAAAGCCA AAATGGCCCGCAGGAACAAGAGGCCTTCAGCTAAAGATGGCCAAGAGAGCAAGAAGCGGAGCTCCCTCTT CCGGAAGATCACAAAACAGTCAAACCTGTTGCACACTAGCCGATCGCTGTCCTCTCTGAACCGGTCTTTG TCGTCCAGCGACAGTCTTCCTGGTTCGCCCACGCACGGACTACCCGCACGCTCGCCCACGCATAGCTATC GCTCCACACCTGACTCCGCCTACCTAGGTGCCTCGTCACAGAGTAGCTCTCCAGCTTCCAGCACACCTAA TTCCCCCGCCTCATCCGCCTCGCACCACATCAGGCCCAGCACTCTGCACGGGCTGTCACCGAAACTACAC CGCCAGTATCGTTCAGCGCGCTGTAAATCGGCTGGCAACATCCCGCTGTCACCTCTGGCGCACACTCCGT CCCCGACGCAGGCGTCGCCGCCGCCGCTGCCAGGCCACACGGTGGGCAGCTCCCATACCACGCAGAGCTT CCCTGCCAAACTGCACTCATCGCCGCCTATCGTGCGTCCGCGCCCCAAAAGTGCAGAGCCACCACGCTCG CCGTTGCTCAAGCGCGTGCAGTCAGCGGAAAAGCTAGGGGCTTCGCTGGGAGCAGACAAGAAGGGAGCAT TGCGCAAACACAGTCTGGAAGTGGGTCACCCTGATTTCCGAAAGGACTTCCACGGTGAGCTGGCGCTGCA TAGCCTCGCTGAGTCCGATGGTGAGACGCCTCCTATTGAGGGTCCTGGGGCGACCCGGCAGGTCGCCATG AGACGCCTGGGCCGCCAGGAATCTCCCCTAAGCTTGGGCGCGGACCCGTTGCTACCCGATGGTGTCCAGA GACCTATGGCGTCAGGCAAGGAGGATTCCGCGGGAGGTACAGAGGCTTGTACTCCACCTCGCGCGACTAC CCCTGGGAGCCGGACCCTGGAGCGTGACTTGGGCTGCACAAGGCACCAGAGCGTGCAGACTGAGGATGGC CCAGGTGGGGTGGCCAGAGCCCTGGCCAAGGCAGCGCTAAGCCCGGTGCAGGAACATGAGACAGGCCGGC GCAGTAGCTCCGGTGAGGCAGGAACACCCCCGGTGCCCATTGTTGTAGAGCCTGCCCGGCCAGGGGTCAA GACCCAGACACCCCAGCCTCTGGGCACAGACTCCAAGGGATTGAAGGAACCTGTAGCCCAGATCCCTCTG GTGCCTGATGCCCCAAGGGTCCGGGAGCGCTGGGTGCTAGAGGAGGTGGAAGAGCGGACCACGTTAAGCG GACCTCGCTCTAAGCCCGCCTCCCCCAAACTCTCTCCTGACCCCCAGACTCCAACCGTAGCCCCAACAAA GAATGTACCCCGCAGTGCAGCTCCTCCAGTCCCACCTGCTTCCCTAATGGTCCCAGGTACGAAGCCCGAA GCAGGGTCGAACTCCCGGTGTCCTGCTGAAGGTGTGGCTTCAGCAGGCCTAACCAAAACGGGGGCACCCA GTCCTGCATCTTTAGGTCCATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_181089
Insert Size	4713 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_181089.1, NP_851603.1
RefSeq Size	4713 bp
RefSeq ORF	4713 bp
Locus ID	353118
UniProt ID	Q810W7
Gene Summary	interacts with syntrophin on the cell surface [RGD, Feb 2006]

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