Usp42 (NM_001105909) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208800 representing NM_001105909 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATAGTTGACAAAACTGAACCTTCAGACCCATCAACCTGTCAGAACCAGCCTGGCAGTTCTGAGG CGGTCTCACCTGAAGACATGGACACAGGCTCTGCCAGCTGGGGTGCCGTGTCTTCCATAAGTGATGTCTC AAATCATACACTTTCATTGGGGCCGGTGCCTGGTGCTGTAGTTTACAATAACTCGTCTGTACCTGAGAAA TCAAAACCATCACCACCAAAGGATCAAGTCCTAGGTGATGGCATTGCTCCTCCTCAAAAGGTCCTATTTC CATCTGAAAAGATTTGTCTTAAGTGGCAACAAAGTCATCGAGTTGGCGCTGGGCTGCAGAATTTGGGCAA TACCTGTTTTGCCAATGCTGCGTTGCAATGTCTGACTTACACACCACCCCTCGCCAACTACATGCTATCC CATGAACACTCCAAGACATGCCATGCAGAAGGATTCTGTATGATGTGCACAATGCAGACGCACATTACCC AGGCACTTAGCAACCCTGGGGATGTTATCAAGCCCATGTTCGTCATCAATGAAATGCGGCGTATAGCTAG ACATTTCCGTTTTGGTAACCAAGAAGATGCCCATGAATTTCTTCAGTACACGGTCGATGCCATGCAGAAA GCATGTTTAAATGGCAGCAATAAATTAGACAGACATACCCAGGCCACCACCCTGGTCTGCCAGATATTTG GAGGCTACCTAAGATCCCGAGTCAAGTGTTTAAATTGCAAGGGTGTTTCAGACACCTTTGATCCATACCT GGACATAACACTGGAGATTAAGGCTGCACAGAGCGTTACCAAGGCGCTGGAACAGTTCGTGAAGCCAGAA CAACTGGATGGAGAAAACTCCTACAAATGTAGCAAGTGCAAAAAGATGGTTCCAGCTTCAAAGAGATTCA CCATCCATAGGTCCTCTAATGTTCTTACCATTTCACTGAAACGGTTTGCCAACTTTACCGGTGGAAAAAT TGCTAAGGACGTGAAATATCCCGAGTACCTTGATATCCGGCCCTATATGTCTCAGCCCAACGGAGAGCCC ATCATTTATGTTTTGTATGCTGTGCTGGTGCACACCGGTTTTAACTGTCATGCTGGCCACTACTTTTGCT ACATCAAGGCTAGCAATGGCCTCTGGTATCAGATGAATGACTCTATCGTGTCCACCAGTGATATTAGATC GGTGCTTAACCAGCAGGCTTACGTGCTCTTTTATATCAGGTCCCATGATGTGAAAAATGGAGGGGAGACC ACTCATCCTGCCCATAGCCCTGGCCAGTCCTCCCCCCGGCCAGGGATCAGTCAGCGTGTAGTCAACAGCA AGCAGGCGGCGCCAGGATTCATCGGGCCGCAGCTGCCTCCCCATGTGATGAAGAACACACCACACTTGAA TGGCACCACGCCGGTGAAAGACACACCAAGCAGTTCTGTTTCAAGCCCTAACGGAAACACCAGCGTCAAT AGGGCCAGTCCTGCTACGGCTTCGACGTCTGTGCAGAACTGGTCTGTTAACAGGCCCTCAGTTATTCCGG AACACCCTAAGAAACAAAAAATTACTATCAGTATTCACAACAAGTTGCCTGTTCGTCAAGGTCAGGCACA GCTGAATAACAACCTCCATGGCCCTTGTCTGGAGGCCCCTAGCAAGGCGGCCCCCTCCTCCACCATCACT AACCCTTCTGCAATACAGTCTACCTCGAATGCACCCACGACATCAGTTTCCCCAAGTGAGTCCTGTCCCA AGCCCATGGTGAATGGCAAGGCTAAAGTGGGCTCCAGTGTGCTCGTCCCCTATGGGGCTGAGTCCTCAGA GGAGTCTGATGAGGAGTCCAAGGGCCTGGCCAAGGAGAACGGTGTGGACATGATGACCAGCACTCAGTCC AGTAGACCAGAAACCGCAGATGAGGACGGTGAGGCTTCCCCTCATGAGCTTCAAGAGTCCGTCATGCTAA ATGGTGCTAATAGCGCAGACAGTGACTCGAGAGAGAGCAGCCTGGCATTTGACAGTGCCAGCTGCCAGGT CCAGCCCGATCTACACACAGAAAACCTCTTTAACAAACTCAATGGTCTTCCTGGAAAGGTGACGCCCGCT CCATTGCAGTCTGTTCCTGAAGACAAAATCCTTGAGACTTTCAAGCTTACCAACCAGGCAAAAGGTCCAG CGGTTGAAGAGAGTTGGACTACAACAGGGGGAAGCTCTCCAAGGGCCTCTGTTTCACAGCCGGAGCCCAT CACCGATGAGACCAGTCCCCTTGAGATACCGGAGGCAGTCACCAACGGGAGCATGAAGACCCCTTCCACC ACGTCACCTCTGGAGCCCACCATCAGCTGTACCAAAGAAGACTCTTCCGTCGTCGTCGTCTCAGCAGAAC CTATGGAGGTTTTGCCTTCCGTCCCTGCTCTCTGTAACAGCACCGGTACTACCTTGGGGGATGCCCCAGT GCCCGAATCGTGTGACCCTGGAGACTTGACTGCCAACCCGAGCCAACCATCGCAAGCGGTGAAGGGTGAT CCAGCTGAGAACTCTGAGAACTCCACCTTGGCTGAAGTGGTGGAGAGGCTGAGCCCTGCTCCCTCTGTGC TCACAGGGGACGGGTGTGAGCAGAAACTCTTCCTTTACCTCAGTACAGAGGGGTCCGAGGAGGCAGAAGA CTCCTCCAGGAGCTCGGCAGTCTCTGCTGACACGGTGCCCCCTCAGCCTGACAGGACCACCAGCTCCTGT GCAGGGGCTGCCGAGCAGGCTGCCGGGGATAGAGGCGATGGAGGCAGTGTGGGACCCAAAGCTCAGGAGC CCTCCCCGGCCAAGGAAAAGATGAACAGCCTTCGGAAAGTGGATCGAGGACACTATCGGAGCCGGAGAGA CCGGTCCTCCAGTGGGGAGCACGTGAGGGACAGCAGGGCCCGACCAGAGGACCATCACCATAAGAAGCGG CGCTGCTACAGCCGAGAGCGGCCCAAGCAGGAGCGCCACCCTGCTAACACATACTGCAATGGGGGCCAGC ACCTGGGCCACGGCGACAGAGCCAGCCCCGAGCGCCGCTCCCTGAGCAGGTACAGCCACCATCACTCACG GGTTAGGAGTGGCCTGGAGCAGGACTGGAGCCGGTACCACCACTCGGAAAATGAGCCTGCCTGGGCCAGG GAGAGGTTCTACCCGGACAAGATGCGCTGGGACAAGTGTAGGTATTACCACGACAGGTACACTACGACCC TGTACACGGCCCGGGAGGCAGCCCGCGAGTGGCGGCCTCTACATGGTCGTGAGCATGAACGCCTTGTCCA GTCTGGACGGCCACACAAGGACAGCTACTGGGGCCGCAAGGGCTGGGACCTACAATCCCGGGGGAAGGAA CGGCCCCACTTCCATAGCCCACGAGAGGCCCCTGGCCTTGCTGTACCCCTCGAGAGGCATCTCCAAGAGA AGGCTGCGCTGAGTGTGCAGGACAGCAGCCACAGTCTCCCTGAGCGCTTTCATGAACACGAAAGTGTCAA GTTGAGGAAAAGGAGGTATGAGACTCTAGACAATAATGACGACCATCCAGAAAAGAAAGTCCACAAAAGC CTGGAGAAGGACGCGCTAGAAGAGCCAAGGGCAAAGAAGCACAAAAAATCTAAAAAGAAAAAGAAGTCCA AAGATAAACACCGGGCTCGGGATAGCAGGCACCAGCAAGAATCCGACTTTTCAGGAGCATACTCTGATGC TGACCTCCATAGACACCGGAAGAAAAAGAAGAAAAAGAAAAGGCATTCAAGGAAGTCGGAGGACTTTATA AAAGATGTTGAGATGCATTTACCGAAGCTCTCGAGCTATGAGGCCGTTGGCCATTTCCGGAGAACAGAGG GCACCTTTCTACTGGCTGATGGTCTGCCTGTGGAAGACAGCGGCCCTTTCCGGGAGAAAACGAAGCATTT AAGGATGGAAAGCCGGCCTGACAGATGTCATCTGTCAGAGTATGGCCAGGGTAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001105909
Insert Size	3978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105909.1, NP_001099379.1
RefSeq Size	5749 bp
RefSeq ORF	3978 bp
Locus ID	288482
UniProt ID	D3ZU96
Gene Summary	Deubiquitinating enzyme which may play an important role during spermatogenesis.[UniProtKB/Swiss-Prot Function]

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