Usp42 (NM_001105909) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208800 representing NM_001105909 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATAGTTGACAAAACTGAACCTTCAGACCCATCAACCTGTCAGAACCAGCCTGGCAGTTCTGAGG CGGTCTCACCTGAAGACATGGACACAGGCTCTGCCAGCTGGGGTGCCGTGTCTTCCATAAGTGATGTCTC AAATCATACACTTTCATTGGGGCCGGTGCCTGGTGCTGTAGTTTACAATAACTCGTCTGTACCTGAGAAA TCAAAACCATCACCACCAAAGGATCAAGTCCTAGGTGATGGCATTGCTCCTCCTCAAAAGGTCCTATTTC CATCTGAAAAGATTTGTCTTAAGTGGCAACAAAGTCATCGAGTTGGCGCTGGGCTGCAGAATTTGGGCAA TACCTGTTTTGCCAATGCTGCGTTGCAATGTCTGACTTACACACCACCCCTCGCCAACTACATGCTATCC CATGAACACTCCAAGACATGCCATGCAGAAGGATTCTGTATGATGTGCACAATGCAGACGCACATTACCC AGGCACTTAGCAACCCTGGGGATGTTATCAAGCCCATGTTCGTCATCAATGAAATGCGGCGTATAGCTAG ACATTTCCGTTTTGGTAACCAAGAAGATGCCCATGAATTTCTTCAGTACACGGTCGATGCCATGCAGAAA GCATGTTTAAATGGCAGCAATAAATTAGACAGACATACCCAGGCCACCACCCTGGTCTGCCAGATATTTG GAGGCTACCTAAGATCCCGAGTCAAGTGTTTAAATTGCAAGGGTGTTTCAGACACCTTTGATCCATACCT GGACATAACACTGGAGATTAAGGCTGCACAGAGCGTTACCAAGGCGCTGGAACAGTTCGTGAAGCCAGAA CAACTGGATGGAGAAAACTCCTACAAATGTAGCAAGTGCAAAAAGATGGTTCCAGCTTCAAAGAGATTCA CCATCCATAGGTCCTCTAATGTTCTTACCATTTCACTGAAACGGTTTGCCAACTTTACCGGTGGAAAAAT TGCTAAGGACGTGAAATATCCCGAGTACCTTGATATCCGGCCCTATATGTCTCAGCCCAACGGAGAGCCC ATCATTTATGTTTTGTATGCTGTGCTGGTGCACACCGGTTTTAACTGTCATGCTGGCCACTACTTTTGCT ACATCAAGGCTAGCAATGGCCTCTGGTATCAGATGAATGACTCTATCGTGTCCACCAGTGATATTAGATC GGTGCTTAACCAGCAGGCTTACGTGCTCTTTTATATCAGGTCCCATGATGTGAAAAATGGAGGGGAGACC ACTCATCCTGCCCATAGCCCTGGCCAGTCCTCCCCCCGGCCAGGGATCAGTCAGCGTGTAGTCAACAGCA AGCAGGCGGCGCCAGGATTCATCGGGCCGCAGCTGCCTCCCCATGTGATGAAGAACACACCACACTTGAA TGGCACCACGCCGGTGAAAGACACACCAAGCAGTTCTGTTTCAAGCCCTAACGGAAACACCAGCGTCAAT AGGGCCAGTCCTGCTACGGCTTCGACGTCTGTGCAGAACTGGTCTGTTAACAGGCCCTCAGTTATTCCGG AACACCCTAAGAAACAAAAAATTACTATCAGTATTCACAACAAGTTGCCTGTTCGTCAAGGTCAGGCACA GCTGAATAACAACCTCCATGGCCCTTGTCTGGAGGCCCCTAGCAAGGCGGCCCCCTCCTCCACCATCACT AACCCTTCTGCAATACAGTCTACCTCGAATGCACCCACGACATCAGTTTCCCCAAGTGAGTCCTGTCCCA AGCCCATGGTGAATGGCAAGGCTAAAGTGGGCTCCAGTGTGCTCGTCCCCTATGGGGCTGAGTCCTCAGA GGAGTCTGATGAGGAGTCCAAGGGCCTGGCCAAGGAGAACGGTGTGGACATGATGACCAGCACTCAGTCC AGTAGACCAGAAACCGCAGATGAGGACGGTGAGGCTTCCCCTCATGAGCTTCAAGAGTCCGTCATGCTAA ATGGTGCTAATAGCGCAGACAGTGACTCGAGAGAGAGCAGCCTGGCATTTGACAGTGCCAGCTGCCAGGT CCAGCCCGATCTACACACAGAAAACCTCTTTAACAAACTCAATGGTCTTCCTGGAAAGGTGACGCCCGCT CCATTGCAGTCTGTTCCTGAAGACAAAATCCTTGAGACTTTCAAGCTTACCAACCAGGCAAAAGGTCCAG CGGTTGAAGAGAGTTGGACTACAACAGGGGGAAGCTCTCCAAGGGCCTCTGTTTCACAGCCGGAGCCCAT CACCGATGAGACCAGTCCCCTTGAGATACCGGAGGCAGTCACCAACGGGAGCATGAAGACCCCTTCCACC ACGTCACCTCTGGAGCCCACCATCAGCTGTACCAAAGAAGACTCTTCCGTCGTCGTCGTCTCAGCAGAAC CTATGGAGGTTTTGCCTTCCGTCCCTGCTCTCTGTAACAGCACCGGTACTACCTTGGGGGATGCCCCAGT GCCCGAATCGTGTGACCCTGGAGACTTGACTGCCAACCCGAGCCAACCATCGCAAGCGGTGAAGGGTGAT CCAGCTGAGAACTCTGAGAACTCCACCTTGGCTGAAGTGGTGGAGAGGCTGAGCCCTGCTCCCTCTGTGC TCACAGGGGACGGGTGTGAGCAGAAACTCTTCCTTTACCTCAGTACAGAGGGGTCCGAGGAGGCAGAAGA CTCCTCCAGGAGCTCGGCAGTCTCTGCTGACACGGTGCCCCCTCAGCCTGACAGGACCACCAGCTCCTGT GCAGGGGCTGCCGAGCAGGCTGCCGGGGATAGAGGCGATGGAGGCAGTGTGGGACCCAAAGCTCAGGAGC CCTCCCCGGCCAAGGAAAAGATGAACAGCCTTCGGAAAGTGGATCGAGGACACTATCGGAGCCGGAGAGA CCGGTCCTCCAGTGGGGAGCACGTGAGGGACAGCAGGGCCCGACCAGAGGACCATCACCATAAGAAGCGG CGCTGCTACAGCCGAGAGCGGCCCAAGCAGGAGCGCCACCCTGCTAACACATACTGCAATGGGGGCCAGC ACCTGGGCCACGGCGACAGAGCCAGCCCCGAGCGCCGCTCCCTGAGCAGGTACAGCCACCATCACTCACG GGTTAGGAGTGGCCTGGAGCAGGACTGGAGCCGGTACCACCACTCGGAAAATGAGCCTGCCTGGGCCAGG GAGAGGTTCTACCCGGACAAGATGCGCTGGGACAAGTGTAGGTATTACCACGACAGGTACACTACGACCC TGTACACGGCCCGGGAGGCAGCCCGCGAGTGGCGGCCTCTACATGGTCGTGAGCATGAACGCCTTGTCCA GTCTGGACGGCCACACAAGGACAGCTACTGGGGCCGCAAGGGCTGGGACCTACAATCCCGGGGGAAGGAA CGGCCCCACTTCCATAGCCCACGAGAGGCCCCTGGCCTTGCTGTACCCCTCGAGAGGCATCTCCAAGAGA AGGCTGCGCTGAGTGTGCAGGACAGCAGCCACAGTCTCCCTGAGCGCTTTCATGAACACGAAAGTGTCAA GTTGAGGAAAAGGAGGTATGAGACTCTAGACAATAATGACGACCATCCAGAAAAGAAAGTCCACAAAAGC CTGGAGAAGGACGCGCTAGAAGAGCCAAGGGCAAAGAAGCACAAAAAATCTAAAAAGAAAAAGAAGTCCA AAGATAAACACCGGGCTCGGGATAGCAGGCACCAGCAAGAATCCGACTTTTCAGGAGCATACTCTGATGC TGACCTCCATAGACACCGGAAGAAAAAGAAGAAAAAGAAAAGGCATTCAAGGAAGTCGGAGGACTTTATA AAAGATGTTGAGATGCATTTACCGAAGCTCTCGAGCTATGAGGCCGTTGGCCATTTCCGGAGAACAGAGG GCACCTTTCTACTGGCTGATGGTCTGCCTGTGGAAGACAGCGGCCCTTTCCGGGAGAAAACGAAGCATTT AAGGATGGAAAGCCGGCCTGACAGATGTCATCTGTCAGAGTATGGCCAGGGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208800 representing NM_001105909 Red=Cloning site Green=Tags(s) MTIVDKTEPSDPSTCQNQPGSSEAVSPEDMDTGSASWGAVSSISDVSNHTLSLGPVPGAVVYNNSSVPEK SKPSPPKDQVLGDGIAPPQKVLFPSEKICLKWQQSHRVGAGLQNLGNTCFANAALQCLTYTPPLANYMLS HEHSKTCHAEGFCMMCTMQTHITQALSNPGDVIKPMFVINEMRRIARHFRFGNQEDAHEFLQYTVDAMQK ACLNGSNKLDRHTQATTLVCQIFGGYLRSRVKCLNCKGVSDTFDPYLDITLEIKAAQSVTKALEQFVKPE QLDGENSYKCSKCKKMVPASKRFTIHRSSNVLTISLKRFANFTGGKIAKDVKYPEYLDIRPYMSQPNGEP IIYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRSVLNQQAYVLFYIRSHDVKNGGET THPAHSPGQSSPRPGISQRVVNSKQAAPGFIGPQLPPHVMKNTPHLNGTTPVKDTPSSSVSSPNGNTSVN RASPATASTSVQNWSVNRPSVIPEHPKKQKITISIHNKLPVRQGQAQLNNNLHGPCLEAPSKAAPSSTIT NPSAIQSTSNAPTTSVSPSESCPKPMVNGKAKVGSSVLVPYGAESSEESDEESKGLAKENGVDMMTSTQS SRPETADEDGEASPHELQESVMLNGANSADSDSRESSLAFDSASCQVQPDLHTENLFNKLNGLPGKVTPA PLQSVPEDKILETFKLTNQAKGPAVEESWTTTGGSSPRASVSQPEPITDETSPLEIPEAVTNGSMKTPST TSPLEPTISCTKEDSSVVVVSAEPMEVLPSVPALCNSTGTTLGDAPVPESCDPGDLTANPSQPSQAVKGD PAENSENSTLAEVVERLSPAPSVLTGDGCEQKLFLYLSTEGSEEAEDSSRSSAVSADTVPPQPDRTTSSC AGAAEQAAGDRGDGGSVGPKAQEPSPAKEKMNSLRKVDRGHYRSRRDRSSSGEHVRDSRARPEDHHHKKR RCYSRERPKQERHPANTYCNGGQHLGHGDRASPERRSLSRYSHHHSRVRSGLEQDWSRYHHSENEPAWAR ERFYPDKMRWDKCRYYHDRYTTTLYTAREAAREWRPLHGREHERLVQSGRPHKDSYWGRKGWDLQSRGKE RPHFHSPREAPGLAVPLERHLQEKAALSVQDSSHSLPERFHEHESVKLRKRRYETLDNNDDHPEKKVHKS LEKDALEEPRAKKHKKSKKKKKSKDKHRARDSRHQQESDFSGAYSDADLHRHRKKKKKKKRHSRKSEDFI KDVEMHLPKLSSYEAVGHFRRTEGTFLLADGLPVEDSGPFREKTKHLRMESRPDRCHLSEYGQGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105909
ORF Size	3975 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105909.1, NP_001099379.1
RefSeq Size	5749 bp
RefSeq ORF	3978 bp
Locus ID	288482
UniProt ID	D3ZU96
MW	146.4 kDa
Gene Summary	Deubiquitinating enzyme which may play an important role during spermatogenesis.[UniProtKB/Swiss-Prot Function]

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