Arfgef2 (NM_181083) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Big2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208780 representing NM_181083 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAAAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCTGACAAGGAGG TGAAGCGGCCCCAGCACTCCCAGCTGCGTAGGGCCTGCCAGGTGGCGCTCGATGAAATTAAAGCAGAACT AGAAAAGCAGAGGCTTGGCGCTGCGGCTCCACCAAAGGCAAACTTCATTGAAGCTGACAAGTATTTCCTT CCATTCGAGCTGGCCTGCCAATCCAAGTCCCCTCGGGTGGTCAGCACGTCCCTGGACTGCTTGCAGAAAC TCATCGCATATGGGCACATCACTGGCAATGCCCCCGACAGTGGTGCTCCTGGGAAGCGGCTGATCGACAG GATTGTGGAGACGGTTTGCAATTGTTTTCAGGGCCCTCAGACAGATGAAGGCGTTCAGCTACAGATAATT AAGGCTCTTCTGACTGCTGTGACTTCCCCACACATTGAAATCCATGAGGGCACCATTCTACAGACAGTGA GGACATGCTACAATATCTATTTGGCCAGCAAAAACCTCATCAATCAAACCACCGCCAAGGCCACGCTCAC CCAAATGCTGAATGTCATCTTCACCCGCATGGAAAACCAAGTGTTACAGGAGGCCAGAGAATTGGAAAAA CCAATCCAGTCAAAACCTCAGTCCCCAGTGATCCAGGCCACAGCAGGATCTCCCAAGTTCAGTCGACTGA AGCAGAGCCAGGCCCAGAGCAAACCAACAACTCCTGAAAAGACAGAATTGCCCAATGGTGACCACGCCAG GAGTAGCCTAGGAAAAGTGAATTCAGAAAATGGAGAGGCTCACCGAGAAAGAGGCTCATCAATCTCCGGT AGAGCTGAGCCTTCTGGGGGATCTGACAATGGAGCCCAGGAGGTGGTGAAGGACATCTTAGAGGACGTGG TCACGTCTGCTGTGAAAGAAGCAGCAGAAAAGCAGGGGCTGCCGGAGCCTGACCAAGCCCCAGGTGTGCC AGAGTGCCAGGAATGCACTGTTCCTCCTGCAGTTGATGAAAACTCACAGACCAATGGCATAGCAGATGAC AGGCAGTCGCTGTCCTCGGCAGATAACCTGGAACCAGATGCTCAAGGACACCCAGTGGCTGCTAGATTCT CTCACATTCTACAGAAGGATGCCTTCCTGGTGTTTCGTTCCCTGTGTAAGCTGTCCATGAAGCCGCTTGG GGAAGGACCACCAGATCCAAAGTCCCACGAGCTTCGTTCCAAGGTGGTTTCCCTGCAGCTGCTGCTCTCT GTGCTGCAGAACGCTGGTCCAGTCTTCAGGAGCCACGAGATGTTCGTCACTGCCATTAAGCAGTACCTGT GTGTAGCCCTTTCCAAGAACGGCGTGTCCTCAGTGCCTGACGTCTTCGAACTCTCACTTGCGATTTTTCT TACTCTTCTTTCAAACTTCAAGATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAGATTTTCCTG AACATTTTAGAAACGTCCACAAGTTCTTTTGAGCACCGCTGGATGGTCATTCAGACCCTGACGAGAATTT GTGCAGATGCCCAGTGTGTTGTGGATATTTATGTCAACTACGACTGTGACTTAAATGCTGCTAACATTTT TGAGCGTCTAGTAAATGATTTATCCAAAATCGCTCAGGGAAGAAGTGGACATGAACTGGGGATGACACCT CTACAGGAGCTCAGTCTGAGGAAGAAAGGCCTGGAGTGTCTGGTGTCCATTCTCAAGTGCATGGTGGAAT GGAGCAAGGACCTGTATGTGAATCCCAACCACCAGGCCACCCTGGGTCAGGAACGGCTTCCAGATCAGGA AATGGGGGACGGAAAAGGCCTTGACATGGCAAGACGGTGCAGCGTGACATCAGTGGAGTCCACAGTGTCC TCGGGGACCCAGACAGCCATTCCAGATGACCCAGAGCAGTTTGAGGTCATCAAACAACAGAAGGAAATCA TCGAGCATGGCATTGAGCTCTTCAATAAGAAGCCCAAGCGGGGAATCCAGTTTCTCCAAGAGCAAGGCAT GCTAGGAGCAGCTGTAGAAGATATTGCCCAATTTCTGCACCAGGAAGAGCGTCTGGATTCTACCCAGGTT GGTGAGTTTCTGGGAGACAGCACAAGGTTTAACAAGGAGGTGATGTATGCCTATGTGGACCAACTGGACT TCTGTGAAAAAGAGTTCGTCTCGGCCCTGAGGACATTCCTGGAGGGTTTCCGCCTGCCTGGGGAAGCCCA GAAGATTGACCGGTTAATGGAGAAGTTTGCTGCACGATATATAGAGTGTAACCAAGGGCAAACCCTGTTT GCTAGTGCTGACACGGCGTACGTCCTGGCCTACTCCATCATTATGCTAACTACAGACCTGCATAGCCCTC AGGTAAAGAATAAAATGACAAAGGAACAGTATATTAAAATGAACCGGGGCATCAACGATAGTAAAGACCT ACCTGAGGAGTACCTGTCCAGCATCTACGAAGAGATAGAAGGCAAGAAGATTGCGATGAAGGAGACCAAG GAGCACACAATGGCAACGAAATCCACCAAGCAGAATGTAGCCAGTGAGAAGCAGAGGCGGCTGCTGTACA ATGTGGAGATGGAGCAGATGGCTAAGACAGCCAAAGCTCTGATGGAGGCCGTGAGCCACGCCAAAGCCCC GTTTACCAGCGCCACACACTTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCACTGCTGGCA GCCTATAGCATCGGCCTACAGAACTGTGACGACACGGAGGTGGCCTCCCTGTGCTTGGAAGGCATCAGGT GTGCAGTCCGCATTGCCTGCATCTTTGGAATGCAGCTGGAACGAGATGCCTATGTTCAGGCCCTTGCTCG CTTTTCCCTGCTGACAGCCAGCTCCAGCATCACAGAAATGAAGCAGAAAAACATTGACACCATAAAGACA CTCATCACTGTGGCTCACACCGATGGCAACTACCTTGGCAACTCCTGGCATGAGATCTTGAAGTGCATCA GCCAGCTGGAGCTCGCTCAGCTCATAGGAACTGGAGTGAAGACTCGCTACCTCTCTGGCTCTGGGCGGGA AAGAGAAGGGAGCCTGAAGGGCCACAGTCTGGCAGGAGAAGAATTCATGGGTCTTGGCCTCGGTAATTTG GTGAGTGGTGGTGTGGATAAAAGACAGATGGCCAGCTTCCAGGAGTCCGTTGGTGAAACCAGCTCACAGA GTGTGGTTGTTGCAGTTGACAGAATTTTTACCGGCTCTACCAGACTGGATGGAAATGCAATAGTTGACTT TGTCCGCTGGCTGTGTGCTGTGTCCATGGATGAGCTGGCTTCTCCCCACCATCCCCGCATGTTCAGCCTG CAGAAGATCGTGGAGATATCATACTACAACATGAACCGGATCCGGCTGCAGTGGTCTCGGATATGGCATG TCATTGGAGATCACTTCAATAAGGTCGGCTGTAACCCCAATGAGGATGTGGCCATCTTTGCTGTTGACTC GCTGCGGCAACTCTCCATGAAGTTTTTGGAGAAGGGAGAATTAGCCAACTTCCGATTTCAGAAGGATTTT CTGAGGCCGTTTGAGCATATTATGAAGAAAAACAGGTCCCCAACCATCCGGGACATGGTCATCCGCTGCA TTGCCCAGATGGTGAGCTCCCAGGCAGCCAACATCCGCTCAGGCTGGAAGAACATCTTTGCTGTGTTCCA CCAGGCCGCCTCTGACCACGATGGGAACATTGTGGAGCTGGCCTTCCAGACCACCGGCCACATTGTCTCA ACCATCTTCCAGCACCACTTCCCTGCGGCCATCGACTCCTTCCAGGACGCTGTGAAGTGCTTGTCTGAGT TTGCCTGCAACGCAGCTTTCCCGGACACCAGTATGGAGGCCATCCGGCTCATCCGCTTCTGTGGGAAATA CGTCTCAGAGAGGCCACGGGTACTGCAGGAGTACACAAGCGATGACATGAATGTGGCTCCTGGTGACAGG GTGTGGGTCAGAGGCTGGTTCCCCATCCTCTTTGAGCTCTCCTGCATCATTAACAGGTGCAAGTTAGATG TGCGCACAAGGGGACTGACAGTCATGTTCGAGATCATGAAGAGCTATGGCCACACCTTTGCAAAGCACTG GTGGCAGGACCTATTCAGAATTGTGTTTCGGATTTTTGACAACATGAAACTCCCTGAGCAACAGTCGGAG AAATCAGAGTGGATGACGACCACCTGCAATCACGCACTGTATGCTATTTGTGACGTATTCACCCAGTTCT ACGAGGCTTTGCATGAAGTGCTTCTCTCCGATGTGTTTGCACAGCTGCAGTGGTGCGTGAAGCAAGATAA CGAGCAGTTGGCCCGCTCGGGTACGAATTGCTTAGAGAACCTAGTGATATCCAATGGAGAGAAGTTCAGC CCTGCTGTCTGGGATGAAACCTGCAATTGCATGTTGGATATTTTCAGAACCACCATCCCACATGTTTTGC TGACGTGGAGACCTGCGGGGATGGAGGAGGAGGTGTCAGATAGACATCTGGACGTGGACCTGGACCGCCA GTCTTTAAGCAGCATAGACAGAAACGCCTCGGAGAGAGGACAGAGCCAGCTGTCTAACCCCACTGATGAC AGCTGGAAGGGAGCACCGTATGCAAATCAGAAACTGCTGGCCAGCCTTCTCATCAAGTGTGTGGTTCAGC TGGAGCTGATACAGACCATTGACAACATCGTGTTCTACCCTGCGACCAGCAAAAAGGAGGATGCAGAGCA CATGGTTGCTGCCCAGCAAGACACACTAGATGCAGATATCCACATTGAGACGGAGAATCAGGGCATGTAC AAGTTCATGTCTTCCCAGCACCTCTTCAAGCTGCTAGACTGCCTGCAGGAGTCCCATTCCTTCTCCAAGG CCTTCAACTCCAACTATGAGCAGCGGACTGTCCTCTGGCGAGCTGGCTTCAAGGGCAAGTCCAAACCAAA TCTCCTCAAACAAGAAACCAGCAGCTTGGCCTGTTGCCTGAGGATCCTGTTTAGAATGTACGTTGATGAG AACCGCAGGGATTCCTGGGGCGAAATACAGCAGCGGCTTTTAACCGTGTGCAGCGAAGCACTTGCCTATT TCATTACTGTGAACTCTGAGAGCCATCGAGAAGCCTGGACGAGCCTCCTCCTCCTCCTTCTAACCAAAAC CCTCAAGATCAACGATGAAAAGTTCAAAGCCCACGCGTCAGTGTACTACCCCTACCTGTGTGAGATGATG CAGTTTGACCTGATCCCGGAGCTCCGAGCAGTTCTGCGAAAGTTCTTCCTGCGAATAGGCCTGGTGTATA AGATATGGGTTCCAGAAGAGCCATCGCAAGTGCCAGCGGCATCGACAGCATGGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_181083
Insert Size	5376 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_181083.2, NP_851597.2
RefSeq Size	5784 bp
RefSeq ORF	5376 bp
Locus ID	296380
UniProt ID	Q7TSU1
Gene Summary	Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.[UniProtKB/Swiss-Prot Function]

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