Igf2r (NM_012756) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	IGF-IIR; Igfr2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208772 representing NM_012756 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCCGTTCTGCCGGGACCCGTGCCCTCCGGGCCGCGCGTCGCGCTCCTGCTGCCGCTCCTGCTGC TGCTGCTCGTGGCGGCCGCGGGCTCCGCGCAGGCCCAGGCCGTGGACTTGGACGCCTTGTGCAGTTACAC ATGGGAAGCTGTTGATTCCAAAAATAATGCAGTTTATAAAATCAACCCCTGTGGACATGTGGATAATCCC AGGTGTGGGCCGACAAGTGCTGTCTGTATGTGTGACCTAAAGTCAGAAAACTGCCGGTCAGTGGGTGACT CACTTTTGAGAAGTTCAGCCAAATCACTCCTGGAATTTAACACCACTACGGGCTGCCAGCCTTCAGAGCA CAGAATCCAGACTAGCATTACATTTCTGTGTGGGAAGACCCTGGGAACTCCTGAATTTGTAACTGCCACA GACTGTGTGCATTACTTCGAATGGAGGACTACTGCAGCCTGCAAGAAAGACATATTTAAAGCTGATAAGG AGGTGCCATGCTATGTATTTGATGACAAGTTACAGAAGCATGATTTGAACCCACTGATCAAGCTGAATGG CGGCTACCTGGTGGATGACTCTGACGCTGACGCATCTCTGTTCATCAATGTGTGTAGAGACATAGACTCC CTTCGGGACCCCAGCACACAGCTGAGAGTCTGCCCTGCTGGCACTGCTGCCTGTCTGCTGAAGGGGAACC AGGCATTTGATGTTGGCCGGCCCAAGGAGGGGCTGAAGCTATTGAGCAAGGACAGGCTCGTTCTGACTTA TGTTAAGGAAGAAGGAGAAAAGCCAGACTTCTGTAATGGTCACAGCCCTGCAGTGACAGTAACATTTGTG TGCCCATCTGAGCGGAGAGAGGGCACCATTCCTAAACTCACTGCCAAGTCCAACTGTCGCTATGAAGTTG AGTGGATCACTGAGTATGCCTGCCACAGAGATTACTTGGAGAGTGAAACCTGCTCCCTCAGCAGTGAGCA GCATGATATTGCTATTGACCTTAGTCCCTTGGCCCAGCATGAAGAAGGGTCCCCCTATGTAGCTGATGGA GGAGAATATAGGTTTTTTATGAATGTCTGTGGGGACACAAAGGTCTCACTCTGTAACAAAGAGGCTGCTG TTTGTCAAGAGAAAAAGGTGGATTCCACTCAAGTCAAAATAGCAGGAAGACACCAGAACCAGACACTCCG GTACTCAGATGGAGATCTCACCCTAATCTATTCTGGAGGTGATGAGTGTAGTTCCGGCTTCCAGCGGATG AGTGTCATAAACTTTGAGTGCAACAAAACCGCAGGTCAAGATGGGAGAGGAGAGCCTGTGTTCACAGGTG AGGTGGACTGCACATACTTCTTCACGTGGGACACTAAATATGCCTGTGTAAAAGAGAAGGAAGATCTCCT CTGCGGGGCCATCGACGGCAAGAAACGTTACGACCTGTCTGTGCTGGCTCGTCACTCAGAATCAGAACAG AATTGGGAAGCAGTGGATGGCAGCCAGGCTGAGTCAGAAAAGAGGAATTTCTTCATCAATGTCTGTCACC GGGTGCTGCAGGCGGGAAAGGCCAAGAACTGTCCTGAAGGTGCTGCCGTGTGTGCTGTGGATAAGAGTGG AAGTAAAAATCTAGGAAAATTTGTCTCTTCTCCTACAAAAGAGAAAGGACACATTCAGCTCTCTTACTCT GATGGCGATGACTGTGGTAATGATAAGAAAATAACAACTAACATCACATTTGTGTGCAAACCAGGTGATC TGGAAAGTGCTCCAGTGCTGAGAGCTGCAGGGCCCGATGGCTGCTCCTATGAGTTTGAGTGGCACACAGC TGCTGCCTGTGTCCTGTCGCAGACGGAGGGAGAAAACTGCACTGTCCTTGATGCTCAGGCAGGGTTTTCT TTTGATTTGTCACTCCTCACAAAGAAGAATGGTGCATATAAAGTTGAGACAGACAAGTATGACTTCTACA TAAATGTTTGTGGCCCTGTATCCGTGAACCTGTGTCAGTCAAACTCAGGAGCCTGCCAGGTAGCAAAAAG TGGTAAGTCTTGGAACTTGGGGCTGAGTAATACTAAGCTTACGTACTATGATGGGATGATCCAGCTGAGC TACAGGAATGGCACGCTGTATAACAATGAAAAGCACACACCGAGATCCACACTGATTACCTTCCTCTGTG ACCGTGATGCTGGAGTGGGCTTCCCAGAATATCAGGAAGAGGACAACTCTACCTACAACTTCCGGTGGTA CACCAGTTATGCTTGCCCGGAAGAGCCCCTGGAGTGCATGGTGACAGACCCCTCCATGATGGAGCAATAT GACCTCTCCAGTCTAGTGAAGTTTGAAGGTGGCCGTGGAGGAAACTGGTATGCCATGGAAAACTCCCGAG AACATTTCACACGGAGGAAATACTACCTCAACGTGTGCCGGCCTCTGAATCCTGTGCCAGGCTGTGATCG ATATGCATCTGCTTGCCAGATGAAATACGAAAACAATGAGGGCTCCTTGGCAGAGACTGTGGCCATCAGT AACTTGGGAGTTGCAAAGACAGGCCCTGTGGTGGAGGAGAGCGGCAGCCTCCTCTTAGAGTATGTGAACG GCTCTGCGTGCACCACCAGTGATGGCCGGCTAACCACCTACAGCACCAGGATCCATCTTGTGTGTGGTCG AGGAACTATGAATTCCCATCCCATATTCACTTTCAACTGGGAATGTGTGGTCAGTTTCTTGTGGAACACG GAGGCTGCCTGCCCCATCCAGACGATCACAGATTCAGACCAGGCTTGCTCTATAAGGGACCCCAACAGCG GATTTGTGTTTAATCTGAGCCCACTTAACTATTCTCAAGGACACATGGTCTTAGGCATTGGGAAGACTTT TGTGTTCAATATCTGTGGTACAATGCCTGCCTGTGGAACGGTTGCAGGGAAGCCAGCCCTTGGCTGTGAG GCAGAAACTAAGATCAAAGACATTAAGGATCTGAAGCCAGAAAGGCCAGTCGGGATGGAGAAGAGCCTCC AGTTGTCTGCCGAGGGGTTCCTCACTCTGACCTACAAAGGCTCTTCTCCTTCTGACAGAGGCACAGCCTT CATCATCCGTTTCATTTGCAATGGTGACATTTACCCAGGGACCCCCAAGTTCCTGCATCAGGACATTGAC TCTGCACGAGGGATCCGGAACACCTTCTTTGAGTTTGAAACTGCATTGGCTTGTATTCCTTCTGTAGTGG ATTGTCAAGTGACTGACCCAGCTGGGAACGAGTACGACCTGAGTGCCCTGAGCATGGTCAGGAAGCCGTG GACTGCTGTGGACACGTCTGTGCATGGGAAGAAGAGGCGCTTCTATCTGAGCGTGTGCACCCCACTGCCT TACATTCCTGGCTGCGATGGCATTGCAATGGGGTCCTGCATGGTGTCAGAAGACAAAAGTCAGAACTTGG GTGTGGTGCAGATCAGTCCTCAAGCTACTGGAAACGGATCTCTCAGCATCCTGTATGTGAATGGGGATAG GTGTGGAAACCAGCGCTACTCCACCAGGATAGTGTTCGAATGTGCCCAGACGTCGGGCTCACCTATGTTC CAGCTCCTGAACAACTGTGAGTATGTGTTTGTGTGGCGAACAGTGGAGGCCTGCCCTGTGGTCAGAGAGG AAGGAGACAACTGCCAGGTGAAAGACCCCAGACACGGCAATTTGTATGACCTGAAGCCCCTGGCCCTCAA TGACACCATCATCAGTGCTGGCGAGTACACCTACTACTTCCGGGTCTGTGGAAAGCTGTCCTTAGATGTG TGCTCTGCCCATGATGGGTCCAAGGCAGTCTCATCATGCCAGGAAAAGAAAGGACCACAAGGATTCCAGA AAGTGGCAGGGCTCCTTAATCAGAAGCTGACTTTCGAAAATGGACTGTTGAAAATGAACTACAGTGGGGG GGACACCTGCCATAAGGTGTATCAGCGCTCCACAACCATCTATTTCTACTGTGACCGGACGACTCAGAAG CCAGTGTTTCTGAAGGAAACTTTGGACTGCTCCTATTTGTTTGAGTGGCGAACACAGTATGCCTGCCCAC CTTTCAATGTGACAGAGTGCTCTATCCAGGATGAGGCTGGCAACTCCATTGACCTCTCCTCACTGTCAAG ATACAGTGACAACTGGGAGGCTGTGACCAGGACAGGGGCCACTGAGCATTACCTCATCAACGTATGCAAG TCTCTGTCTCCACAGGCTGGCACAGATCCATGCCCTCCAGAGGCAGCTGTTTGTCTGCTGGATGGCTCCA AGCCTGTGAACCTCGGCAGGGTGAGGGATGGTCCTCAGTGGACAGCTGGTGTGACTGTCCTGAAGTATGT GGATGGTGACTTGTGCCCCGACAAAATTCGGAAAAGGTCAACCATTATTCGCTTCACCTGCAGTGACAGC CAAGTGAACTCCAGGCCTCTGTTCATCAGTGCTGTACAGGACTGTGAGTACACATTTTCCTGGCCCACAC CTGCTGCCTGTCCTGTGAAGAGCAACATACACGATGACTGCCAAGTCACCAACCCCAGCACAGGCCACCT GTTTGACCTGAGCTCCCTAAGTGGCAAAGCTGGCATCACAGCCTCCTACAGCGAGAAGGGGATGGTCTTC ATGAGCATCTGTGAGGAGAATGTGAACTGTAGCCCTGGAGTAGGGGCCTGCTTTGGACAGACCAGGATCA GTGTGGGCCAGGCCAGCAAGAGGCTGAGCTACAAGGATCAGGTCTTGCAGCTGGTGTATGAGAATGGGTC CCCATGCCCCTCCAAGTCAGGCCTGAGGTACAAGAGTGTCATCAGCTTCGTGTGCCGGCCGGAGGCTGGA CCCACCAACAGGCCCATGCTCATCTCCTTGGACAAGCAGTCGTGTACACTCTTCTTCTCCTGGCACACGC CCCTGGCCTGTGAGCAGGCGACGGAATGCACCGTGCGGAATGGAAGCTCGATTATTGACCTGTCACCTCT CATCCACCGCACTGGTGGTTATGAAGCATACGATGAGAGTGAGGACGACACCTCTGATACCACCCCTGAC TTCTACATCAACATCTGTCAGCCACTGAATCCCATGCATGGAGTGCCCTGCCCTGCAGGCGCCTCAGTAT GCAAAGTCCCCGTGGACGGGCCCCCTATAGATATTGGGAGAGTCACAGGCCCTCCAATATTCAATCCCGT GGCCAATGAGGTCTACCTGAACTTTGAGAGCAGCACTCCTTGCTTAGCGGACAAATATATGAACTACACC TCACTAATTGCGTTTCACTGCAGGAGAGGGATCAGCATGGGAACTCCTAAGCTGATAAGGAACAATGACT GTGACTTTGTGTTTGAGTGGGAGACTCCGATTGTCTGTCCTGACGAAGTGAAGACACAGGGCTGTGCTGT AACAGATGAGCAGCTGCTCTATAGCTTCAACCTGACGAGCCTTTCCACAAGCACTTTCAAAGTCACTCGG GACGCTCACACATACAGTATAGGGGTGTGCACAACGGCAGCTGACTTAGACCAGGAAGGCTGCAAGGACG GCGGGGTCTGTCTGCTCTCTGGGAGCAAAGGAGCTTCTTTTGGGCGCCTAGCGTCGATGCAGCTGGATTA CAGGCACCAGGATGAAGCAGTCATCCTGAGTTATGTGAACGGTGATCCTTGCCCTCCAGAAACGGAAGAT GGCGAGCCCTGTGTCTTCCCCTTCATTTACAAAGGGGAGACCTATGACGAATGTGTATTAGAGGGACGAG CGAAACTGTGGTGTAGCAAAACTGCGAATTACGATCGTGACCACGAGTGGGGCTTCTGCAGACCGAGCAA CAGTCACCGGATGTCTGCGATCATATTTACTTGTGATGAGAACGAAGATATTGGGAGGCCAGAAGTGTTC AGTGAAGATCGTGGGTGTGAGGTGACATTTGAGTGGAAAACAAAAGTTGTCTGCCCTCCGAAGAAGATGG AGTGCAAGTTTGTTCAGAAGCATAAAACCTATGACTTGCGATTGCTCTCATCCCTCACGGGGTCCTGGGA TTTTGTGCATGAAGGAAACTCGTACTTTATCAATCTGTGCCAAAGAGTATATAAAGGTCCCCTGGACTGC TCTGAGAGAGCCAGCATTTGCAAGAAGAGTGCCACTGGTCAAGTCCAGGTTTTGGGGCTTGTTCATACTC AAAAACTGGAAGTCATAGATGAAACAGTCCTTGTCACTTACTCAAAAGGCCATTCTTGTGGTGGGAATAA GACTGCATCCTCAGTGATCGAGTTGACCTGTGCAAAGACTGTGGGCAGGCCTGCATTCAAGAGGTTTGAT ATTGACAGCTGCACCTACTACTTTTACTGGTACTCACGGGCTGCCTGTGCTGTGAGACCTCAGGAGGTGA ACATGGTGAATGGAACTCTTACCAACCCTGTGACTGGCAAGAGCTTCAGCCTCGGGGAAATTTATTTTAA GCTGTTCAGCGCCTCTGGGGACATGAGAAGCAATGGGGACAACTATCTGTATGAGATCCAGCTCTCCTCT ATCTCCAGCTCTTCTAACCCTGCGTGCTCCGGTGCCAACATCTGCCAGGTGAAGCCCAATGACCAGCACT TCAGCAGGAAAGTGGGCACCTCTGACATGACCAAGTACTACGTCCAAGACGGTGATCTGGATGTGGTGTT TACCTCTTCCTCCACGTGTGGCAAAGATAAGACCAAGTCTGTGTCTTCCACCATCTTCTTCCACTGTGAC CCTCTGCTGAAGGACGGGGTCCCCGAGTTCAGCCACGAGACTGCTGACTGCCAGTACCTTTTCTCCTGGT ACACTTCAGCTGTGTGTCCTCTGGGTGTGGACTTTGATGATGAGAATGCTGTGCCAGAGTATAAAGGGCT CTCAGAGCGGAGTCAGGCCGTTGGGGCAGTCCTCAGTCTCCTCCTGGTGGCACTGACTGGCTGTCTGCTG GCCTTGCTGCTTCATAAGAAGGAGAGAAGGGAAACTGTGATAAACAAGCTGACCAATTGCTGCAGAAGGA GCTCGGGGGTGTCCTATAAGTACTCAAAGGTCAGCAAGGAGGAGGAGACAGATGAGAATGAGACAGAATG GCTGATGGAAGAGATCCAGGTGCCAGCCGCCAGACTAGGCAAGGACGGGCAGGAGAACGGCCACATTACC ACCAAGACGGTGAAAGCAGAAGCTCTCACTTCCCTGCATGGGGATGACCAAGACAGTGAGGATGAGGTCC TGACCATCCCAGAGGTGAAAGTTCACACAGGCAGAGGAGCCGAGGTGGAAAGCTCACAGCCCCTGAGAAA TCCCCAGCGCAAGGTCCTGAAGGAAAGGGAGGGGGAGAGGATGGGGCTGGTCCGCGGTGAGAAGGCCAGA AGAGGGAAGTTCAGACCCGGACAGCGCAAGCCAACAACCCCTGCCAAGCTGGTGTCCTTCCATGATGACA GTGACGAGGACCTCCTACACATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012756
Insert Size	7446 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012756.2, NP_036888.2
RefSeq Size	8803 bp
RefSeq ORF	7446 bp
Locus ID	25151
Gene Summary	mediates internalization of insulin-like growth factor II; plays a role in lysosomal enzyme transport [RGD, Feb 2006]

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