Map3k4 (NM_001107456) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208609 representing NM_001107456 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACACCGAGTCAGTCTCCACATAAAGACTTGGGGAAGGGAGTGGAGGCCGTGGAGGAGTACAGCT ACAAGCAGGGGAAGAAGACGCGAGCGACTCTGAGGACAACAGAGCGAGACCATAAGAAAAATGCACAGTG CTCGTTCATGTTGGACTCAGTGGCCGGGTCTTTGCCAAAAAAATCTATTCCAGATGTGGATCTCAATAAG CCTTACCTCAGCCTCGGCTGTAGCAACGCCAAGCTGCCAGTCTCTATGCCCATGCCTATAGCCAGAACTG CGCGGCAGACTTCCCGGACGGACTGCCCAGCGGATCGGTTAAAGTTCTTTGAAACACTGCGCCTTTTGCT AAAGCTTACCTCAGTCTCGAAGAAGAAGGACAGGGAACAGAGGGGACAGGAAAACACGGCTGCTTTCTGG TTCAACCGGTCAAATGAACTGATCTGGTTAGAGCTCCAGGCCTGGCACGCAGGCCGCACCATCAATGACC AGGACCTCTTTTTATACACAGCCCGCCAAGCCATCCCAGATATTATCAATGAAATCCTCACATTCAAAGT TAATTATGGAAGCGCTGCCTTCTCTAGAAATGGAGCTGGTTTCAACGGTACCTCGCTAGAAGGTCAGTGC AGAACCCTTCATGGGACGAACAGTCTAGGCTGTTCAACCTACCACGAGCACCTCCAGCGCCAGAGGGTCT CATTTGAGCAGGTGAAACGGATAATGGAGCTGCTAGAGTACATGGAAGCACTTTATCCGTCCTTGCAGGC TCTGCAGAAGGACTACGAGAGATACGCCGCAAAGGACTTTGAGGACAGAGTGCAGGCACTCTGCCTGTGG CTAAACATCACGAAAGATTTAAATCAGAAGTTGAGGATTATGGGCACTGTTCTGGGCATCAAGAACTTAT CAGACATTGGCTGGCCAGTGTTTGAAATCCCTTCCCCTCGGCCATCCAAAGGCTACGAGCCTGAGGAGGA GGGCGAGGACACAGAGGGAGAGCTGAGGGAGCTGGAGAGTGGGACGGAGGAGAGTGATGAGGAGCGAAGT CCCAATCCGAGGGTGCCAGAGCTCAGGCTGTCTGCCGACACCATCTTGGACAGTCACTCCCAGGGCTGCG CGTCCAGGAAACTTGAGAGGCTCGAGTCCGAGGAGGACTCTGTAGGCTGGGGGATAGCAGACTGTGGTCC CGAAGCCAGTAGGCATTGTTTGACTTCTATCTATAGACCGTTCGTGGACAAAGCACTGAAGCAAATGGGG CTGAGGAAGTTAATTTTACGACTTCATAAGCTAATGAATGGCTCCTTGCAAAGGGCTCGTGTAGCTCTGG TAAAGGATGACCGTCCTGTGGAGTTCTCTGACTTTCCAGGTCCCATGTGGGGCTCGGATTATGTGCAGTT GTCGGGGACGCCTCCTTCCTCGGAGCAGAAGTGCAGTGCTGTGTCCTGGGAAGAGCTGAGGGCCATGGAC CTGCCCTCCTTTGAGCCCGCCTTCCTGGTGCTCTGCCGGGTCCTGCTGAACGTGATCCATGAGTGCCTGA AGCTGCGGCTGGAACAGAGGCCGGCCGGGGAGCCTTCCCTCTTGAGTATCAAGCAGGTGTATTTTGACTA CATGAGAAGCTGGATCCAAATGCTACAGCAGTTACCTCAGGCTTCCCATAGCTTAAAAAACCTGCTAGAA GAAGAATGGAATTTCACCAAAGAAATAACCCATTACATCCGGGGAGGAGAAGCACAGGCTGGAAAGCTTT TCTGTGACATTGCAGGGATGCTGCTGAAATCCACGGGGAGCTTTCTGGACTCTGGCCTACAGGAGAGCTG TGCTGAACTGTGGATCAGCGCCGACGACAACGGTGCTGCCGACGAGCTAAGGAGGTCTGTCATCGAGATC AGCCGAGCACTCAAGGAGCTCTTCCATGAGGCCCGGGAAAGAGCCTCCAAGGCCCTGGGCTTTGCTAAAA TGCTGAGGAAGGACCTAGAAATAGCAGCAGAGTTTGTTCTGTCTGCGTCTGCCCGAGAGCTCCTAGACGT TCTGAGAGCAAAGCAGTATGTTAAGGTACAGATTCCTGGGCTAGAGAATTTGCACGTGTTTGTCCCCGAC AGCCTCGCTGAGGAGAAGAAAATTATTTTGCAGCTACTCAATGCTGCCACAGGAAAGGACTGCTCAAAGG ATCCGGACGACGTCTTTGTGGATGCCTTCCTGCTCCTGACCAAGCATGGGGACCGAGCCCGTGACTCAGA AGATGGCTGGAGCACATGGGAAGCACGAGCTATCAAAATTGTGCCTCAGGTGGAGACCGTGGACACCCTG AGAAGCATGCAGGTGGACAACCTTCTGCTGGTTGTCATGGAGTCTGCTCACCTCGTACTTCAGAGAAAGG CCTTCCAGCAGTCCATTGAGGGGCTGATGACTGTACACCATGAGCAGACATCCAGCCAGCCAGTCATTGC CAAAGCTTTACAGCAGCTAAAGAACGATGCGCTCGAGCTGTGCAACAGAATAAGCGATGCCATCGACCGC GTGGACCACATGTTCACGCTGGAGTTGGATGCCGAGGTCGAGGAGTCCGAGTCGGCCACGCTGCAGCAGT ACTACCGAGAGGCCATGATTCAGGGCTACAACTTCGGGTTTGAGTATCATAAAGAAGTTGTTCGTTTGAT GTCTGGAGAATTCAGACAGAAGATAGGAGACAAATATATAAGCTTTGCCCAGAAGTGGATGAATTATGTG CTGACCAAATGTGAGAGCGGCAGAGGCACAAGACCCAGATGGGCCACCCAAGGTTTTGATTTCCTACAAG CCATTGAACCTGCCTTTATTTCAGCTTTACCAGAAGATGACTTCTTGAGTTTGCAAGCCCTGATGAATGA GTGCATTGGGCACGTCATAGGAAAGCCACACAGCCCTGTCACAGCTATCCATCGGAACAGCCCCCGCCCT GTGAAGGTGCCCCGATGCCACAGTGACCCTCCTAACCCTCACCTCATCATCCCAACTCCAGAGGGATTCA GGGGTTCCAGCGTCCCTGAAAATGACCGCTTGGCCTCCATAGCTGCAGAACTGCAGTTCAGGTCTCTGAG TCGGCACTCGAGCCCCACGGAAGAGCGAGACGAGCCAGCATATCCTCGGAGCGACTCAAGTGGATCAACA CGGAGAAGCTGGGAACTTCGAACACTCATCAGCCAGACCAAAGACTCGGCCTCTAAGCAGGGGCCCATAG AGGCCATCCAGAAGTCAGTCCGACTGTTTGAAGAGAGGAGGTATCGAGAGATGAGGAGGAAGAATATCAT CGGTCAAGTGTGCGATACCCCTAAGTCCTATGATAACATCATGCATGTTGGACTGAGGAAGGTGACGTTT AAGTGGCAAAGAGGAAACAAAATCGGAGAAGGGCAGTATGGAAAAGTGTACACCTGCATCAGCGTCGACA CAGGGGAGCTGATGGCCATGAAGGAGATTCGATTTCAGCCTAACGACCATAAGACTATCAAGGAAACTGC AGACGAGTTGAAAATATTTGAAGGCATCAAGCACCCCAACCTGGTCCGGTACTTTGGTGTGGAGCTTCAC AGGGAAGAGATGTACATCTTCATGGAGTACTGTGATGAAGGCACACTAGAGGAGGTGTCAAGGCTGGGCC TTCAGGAGCACGTCATCCGGTTGTATTCCAAGCAGATCACTGTCGCCATCAACGTCCTCCATGAGCATGG CATCGTTCACCGAGACATCAAAGGTGCCAATATCTTCCTTACATCGTCTGGACTAATCAAACTGGGAGAT TTTGGATGCTCAGTGAAACTTAAAAACAATGCTCAGACCATGCCTGGCGAGGTGAACAGCACCCTAGGGA CAGCAGCTTACATGGCCCCTGAAGTTATTACCCGAGCTAAAGGAGAAGGCCACGGACGTGCGGCAGACAT CTGGAGTCTGGGGTGCGTCGTCATAGAGATGGTGACTGGCAAGCGGCCTTGGCATGAGTTTGAACACAAC TTTCAGATTATGTACAAGGTGGGAATGGGACACAAGCCACCAATCCCCGAAAGGTTAAGCCCTGAAGGAA AGGACTTTCTCTCGCACTGCCTGGAAAGTGACCCGAAGATAAGGTGGACAGCCAGCCAGCTCCTTGACCA CGCTTTTGTCAAGGTTTGCACAGATGAAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107456
Insert Size	4164 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107456.1, NP_001100926.1
RefSeq Size	4908 bp
RefSeq ORF	4164 bp
Locus ID	308106

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