Cbs (NM_012522) Rat Untagged Clone
CAT#: RN208308
Cbs (untagged ORF) - Rat cystathionine beta synthase (Cbs), (10 ug)
CNY 6,120.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN208308 representing NM_012522
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCAGGGACATCCCAGTGTGAAGATGGCTCTGCAGGGTGCCCCCAGGACTTGGAGGTACAGCCAG AAAAAGGGCAACTGGAGAAGGGAGCCTCAGGGGACAAGGAAAGAGTCTGGATCTCGCCTGATACCCCAAG CAGATGTACTTGGCAGCTGGGCAGGCCCATGGCGGATTCCCCACATTACCACACAGTGCCGACAAAATCC CCGAAAATTTTGCCAGATATTCTGAGGAAAATTGGCAACACCCCTATGGTCAGAATCAACAGGATCTCCA AGAATGCAGGACTCAAGTGCGAGCTGTTGGCCAAGTGTGAGTTCTTCAACGCCGGTGGGAGTGTGAAGGA CCGCATCAGCCTCCGGATGATTGAAGACGCTGAGCGAGCCGGAACCTTGAAGCCCGGAGACACGATCATT GAGCCAACTTCTGGCAACACAGGGATCGGGCTGGCTCTGGCAGCTGCTGTGAAGGGCTATCGCTGCATTA TCGTGATGCCTGAGAAGATGAGTATGGAGAAGGTGGATGTGCTGCGAGCTCTGGGAGCTGAGATTGTGAG GACGCCCACCAACGCCAGATTCGATTCCCCCGAGTCCCACGTAGGAGTGGCATGGCGACTGAAGAACGAA ATCCCCAATTCTCACATTCTGGACCAGTACCGCAATGCCAGCAACCCCTTGGCGCACTACGATGACACCG CAGAGGAGATCCTGCAGCAGTGCGACGGGAAGGTGGACATGCTGGTGGCTTCAGCAGGCACGGGTGGCAC CATCACGGGTATCGCGAGGAAGCTGAAGGAGAAGTGCCCAGGTTGTAAAATCATCGGTGTAGATCCCGAG GGGTCCATCCTCGCGGAGCCCGAGGAGCTGAACCAGACGGAGCAAACAGCCTATGAGGTGGAAGGGATCG GCTACGACTTCATCCCCACCGTCCTGGACAGGGCGGTGGTGGATAGGTGGTTCAAGAGCAATGATGACGA TTCCTTCGCCTTCGCCCGCATGCTCATCTCCCAGGAGGGACTGCTGTGCGGTGGGAGTTCAGGCAGCGCT ATGGCCGTGGCTGTGAAGGCTGCCCAGGAGCTAAAGGAAGGACAGCGCTGTGTGGTCATCCTGCCCGACT CTGTGCGCAACTACATGTCCAAGTTCTTGAGTGACAAATGGATGCTGCAGAAAGGCTTCATGAAGGAGGA GCTCTCCGTGAAGAGACCCTGGTGGTGGCATCTGCGTGTCCAAGAGCTGAGCCTATCAGCACCGCTGACC GTGTTGCCCACTGTCACCTGTGAGCACACCATCGCCATCCTCCGGGAGAAGGGTTTTGACCAGGCACCTG TGGTCAACGAGTCTGGGGCCATCCTAGGGATGGTGACTCTCGGGAACATGTTGTCCTCCCTGCTTGCTGG GAAGGTGCGGCCATCAGACGAAGTCTGCAAAGTCCTCTACAAGCAGTTCAAGCCGATCCACCTGACCGAC ACACTGGGCATGCTCTCCCACATCCTGGAGATGGACCACTTCGCCCTGGTGGTCCATGAGCAGATCCAAT ACCGCAACAATGGCGTGTCCAGCAAGCAGCTGATGGTGTTTGGTGTTGTCACCGCCATTGACCTGCTAAA CTTCGTGGCAGCCCGTGAGCAGACCCGGAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_012522 |
Insert Size | 1644 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012522.2, NP_036654.2 |
RefSeq Size | 2539 bp |
RefSeq ORF | 1644 bp |
Locus ID | 24250 |
UniProt ID | P32232 |
Gene Summary | catalyzes condensation of serine and homocyteine to cystathione; involved in transsulfuration pathway; regulated by insulin; alternative splicing produces four mRNA species [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR208308 | Cbs (Myc-DDK-tagged ORF) - Rat cystathionine beta synthase (Cbs), (10 ug) |
CNY 7,030.00 |
|
RR208308L3 | Lenti ORF clone of Cbs (Myc-DDK-tagged ORF) - Rat cystathionine beta synthase (Cbs), (10 ug) |
CNY 8,930.00 |
|
RR208308L4 | Lenti ORF clone of Cbs (mGFP-tagged ORF) - Rat cystathionine beta synthase (Cbs), (10 ug) |
CNY 9,790.00 |